Abstract:
:In the present study, a recombinant aminopeptidase P (rTgAPP) from Toxoplasma gondii was expressed in Escherichia coli to evaluate its enzyme parameters. The rTgAPP showed strong activity against a synthetic substrate for aminopeptidase P at pH 8·0 with a K m value of 0·255 µ m and a k cat value of 35·6 s-1. The overall catalytic efficiency (k cat/K m) of the rTgAPP was 139·6 × 105 M-1 s-1. The activity of rTgAPP was enhanced by the addition of divalent cations and inhibited by bestatin. Deletion of TgAPP gene in the parasite through a CRISPR/Cas9 system resulted in inhibition of growth indicating the importance of TgAPP. Thus our findings reveal that TgAPP is an active enzyme in T. gondii and provide an insight into the function of TgAPP.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Yang M,Zheng J,Jia H,Song Mdoi
10.1017/S0031182016000986subject
Has Abstractpub_date
2016-09-01 00:00:00pages
1443-9issue
11eissn
0031-1820issn
1469-8161pii
S0031182016000986journal_volume
143pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::Avian malaria is a vector transmitted disease caused by Plasmodium and recent studies suggest that variation in its prevalence across avian hosts is correlated with a variety of ecological traits. Here we examine the relationship between prevalence and diversity of Plasmodium lineages in southeastern Amazonia and: (1)...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118201700035X
更新日期:2017-07-01 00:00:00
abstract::The effects of a parasitic infection with the nematode Nippostrongylus brasiliensis on the degradation rates of cytoplasmic tRNA, rRNA and mRNA in rats have been investigated by measuring the renal excretion rates of the modified RNA catabolites N6-threoninocarbonyladenosine, pseudouridine and 7-methylguanine. Between...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182097002102
更新日期:1998-02-01 00:00:00
abstract::The mechanism by which Giardia lamblia exerts its pathogenicity is likely to be multifactorial. A 58 kDa enterotoxin was purified and characterized from the excretory-secretory product (ESP) of the parasite (Kaur et al. 2001). In the present study an attempt has been made to elucidate the mechanism of action of the ES...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004005852
更新日期:2004-10-01 00:00:00
abstract::To test the homogeneity of 18S sequences within Trypanosoma cruzi, riboprint profiles were separated by temperature gradient gel electrophoresis (TGGE). Upon interpretation of melting curves of fragments within a riboprint profile, there appeared to be two 18S sequence types within each stock examined. Two similar typ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098002972
更新日期:1998-09-01 00:00:00
abstract::Prevention of heartworm disease caused by Dirofilaria immitis in domestic dogs and cats relies on a single drug class, the macrocyclic lactones (MLs). Recently, it has been demonstrated that ML-resistant D. immitis are circulating in the Mississippi Delta region of the USA, but the prevalence and impact of these resis...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S003118201500061X
更新日期:2015-09-01 00:00:00
abstract::We studied the component community of lung-worms of the harbour porpoise, attempting to establish the relative importance of ecological and evolutionary factors on its development. The lungs of 64 porpoises by-caught in Norwegian waters were examined for helminths. Three pseudaliid species were detected. The porpoises...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076186
更新日期:1994-04-01 00:00:00
abstract::In order to investigate the phenomenon of age-related immunity to visceral leishmaniasis, a 1 year prospective survey was carried out on 5671 people in a Leishmania infantum focus in north-west Iran. The average incidence rate of infection since 1985 was 2.8%/year with all ages equally at risk. One in 13 infections in...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099004680
更新日期:1999-09-01 00:00:00
abstract::Laboratory experiments indicated that the active life-span of the cercariae of Cryptocotyle lingua was temperature-dependent. An increase in water temperature and population age both correlated with a tendency for the organisms to become decaudate. The larvae were strongly photoresponsive to lateral light but with tim...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000073789
更新日期:1992-08-01 00:00:00
abstract::Digenean parasites infecting four Cominella whelk species (C. glandiformis, C. adspersa, C. maculosa and C. virgata), which inhabit New Zealand's intertidal zone, were analysed using molecular techniques. Mitochondrial 16S and cytochrome oxidase 1 (COI) and nuclear rDNA ITS1 sequences were used to infer phylogenetic r...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000494
更新日期:2016-08-01 00:00:00
abstract::Vertically transmitted parasites (VTPs) such as Wolbachia are expected not only to minimize the damage they inflict on their hosts, but also to protect their hosts against the damaging effects of coinfecting parasites. By modifying the fitness costs of the infection, VTPs can therefore play an important role in the ev...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017001330
更新日期:2018-05-01 00:00:00
abstract::The blackcap (Sylvia atricapilla) is a common Palearctic migratory warbler, and haemosporidian parasites are common in this species. However, genetic and phenotypic diversity of haemosporidians in warblers has been insufficiently investigated and poorly linked. We addressed this issue by combining molecular and micros...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009991235
更新日期:2010-02-01 00:00:00
abstract::There has been an enormous growth in the development of biotechnological applications, where advances in the techniques of microelectronic fabrication and the technologies of miniaturization and integration in semiconductor industries are being applied to the production of Laboratory-on-a-Chip devices. The aim of this...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182099004126
更新日期:1998-01-01 00:00:00
abstract::The dynamic interaction of cells of the immune system with other cells, antigens and secreted factors determines the nature of an immune response. The response of individual cells is governed by the sequence of intracellular signalling events triggered following the association of cell surface molecules during cell-ce...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182005008115
更新日期:2005-01-01 00:00:00
abstract::Antibodies against variable antigens expressed on the surface of Plasmodium falciparum-infected erythrocytes are believed to be important for protection against malaria. A target for these antibodies is the P. falciparum erythrocyte membrane protein 1, PfEMP1, which is encoded by around 50 var genes and undergoes clon...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099004485
更新日期:1999-07-01 00:00:00
abstract::The structure and development of microgametocytes pf Eimeria maxima from chicks killed during the 6th day of infection were examined using transmission and scanning electron microscopy. Initially, nucleoli disappear and the surface of the microgametocyte invaginates. This is followed by a condensation of the chromatin...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000041937
更新日期:1981-02-01 00:00:00
abstract::Although the rumen fluke, Calicophoron daubneyi is now very common and widespread throughout Western Europe, reports of clinical cases are still rare. This study explores the epidemiological background to a severe rumen fluke outbreak in 6-month-old heifers on a dairy farm in Ireland. Sequence analysis of the cytochro...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017002086
更新日期:2018-06-01 00:00:00
abstract::Harnessing the full utility of extensive gene sequences recently available for the economically important sea louse, Lepeophtheirus salmonis, requires the adaptation of modern molecular biology approaches to this non-model organism. Using a putative microsomal prostaglandin E synthase type-2 (PGES2) as a candidate gen...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009990357
更新日期:2009-07-01 00:00:00
abstract::Essential oils (EOs) are considered a new class of ecological products aimed at the control of insects for industrial and domestic use; however, there still is a lack of studies involving the control of fleas. Ctenocephalides felis felis, the most observed parasite in dogs and cats, is associated with several diseases...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019001641
更新日期:2020-03-01 00:00:00
abstract::Nematophagous fungi are used in the biological control of the parasitic helminths of animals and plants. The association of ovicidal and predator nematophagous fungi may present a complementary and increased action on the biological control of helminths. Joint growth compatibility and predation tests were carried out ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118201900060X
更新日期:2019-09-01 00:00:00
abstract::To compare the structures of natural and re-established populations of Ascaris in humans, universal (mass) chemotherapy was carried out at the beginning and the end of the study year using pyrantel pamoate. Worms expelled within 48 h of treatment were collected, their sex determined, and measurements made of length, w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182002001737
更新日期:2002-06-01 00:00:00
abstract::Parasites can have a wide range of pathophysiological effects on the host. This review describes those associated with some parasites of major importance in man and animals. Haemoprotozoan diseases such as trypanosomiasis and malaria are primarily associated with anaemia. Such anaemias have a complex aetiology involvi...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000085814
更新日期:1987-01-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular protozoan parasite. The objective of the present study was to examine the behaviour of Toxoplasma gondii RH Ankara strain tachyzoites in a cell culture environment. The study represents the first step in determining whether T. gondii RH Ankara strain tachyzoites, grown in...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005009078
更新日期:2006-03-01 00:00:00
abstract::Bat flies are obligate ectoparasites of bats and it has been hypothesized that they may be involved in the transmission of Bartonella species between bats. A survey was conducted to identify whether Cyclopodia greefi greefi (Diptera: Nycteribiidae) collected from Ghana and 2 islands in the Gulf of Guinea harbour Barto...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011002113
更新日期:2012-03-01 00:00:00
abstract::Humans and other hosts acquire Toxoplasma gondii infection by ingesting tissue cysts in undercooked meat, or by food or drink contaminated with oocysts. Currently, there is no vaccine to prevent clinical disease due this parasite in humans, although, various T. gondii vaccine candidates are being developed. Mice are g...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001673
更新日期:2012-01-01 00:00:00
abstract::Pasteuria penetrans is a Gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. Attachment of endospores to the cuticle of the nematode is the first stage in the infection process. Western blot analysis with monoclonal and polyclonal antibodies that recognize the 30 kDa heparin-binding ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001008411
更新日期:2001-09-01 00:00:00
abstract::Ocular, peroral, intraperitoneal, intramuscular, and subcutaneous inoculation of severe combined immunodeficient (SCID) mice with spores of the human isolate (CDC: V404) of Brachiola algerae (syn. Nosema algerae) (Phylum Microspora) revealed that the microsporidium develops in viscera of the immunodeficient mouse host...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001008228
更新日期:2001-08-01 00:00:00
abstract::Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of p...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008504
更新日期:2005-12-01 00:00:00
abstract::Stratospheric ozone depletion threatens to increase exposure to ultraviolet (UV) radiation which is known to be a factor in a number of diseases. There is little doubt that cumulative exposure to UV radiation is important in the aetiology of non-melanoma skin cancers. Evidence is also strong for a link with cutaneous ...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000086108
更新日期:1993-01-01 00:00:00
abstract::Intraerythrocytic malaria parasites ingest the cytosol of their host cell and digest it inside their acid food vacuoles. Acidified (pH 4-5.5, 37 degrees C) human red blood cell lysates were used to simulate this process, measuring the denaturation of haemoglobin (Hb) and the release of iron, in the absence or presence...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000075910
更新日期:1994-05-01 00:00:00
abstract::Several approaches were adopted for the disruption and removal of the tegumental surface from protoscoleces of the horse strain of the hydatid organism, Echinococcus granulosus. The effectiveness of each method and the purity of subsequent microthrix-enriched fractions obtained by differential centrifugation were eval...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000049064
更新日期:1985-02-01 00:00:00