The diagnosis of malaria infection using a solid-phase radioimmunoassay for the detection of malaria antigens. Application to the detection of Plasmodium berghei infection in mice.

abstract：:Genome sequencing has greatly contributed to our understanding of parasitic protozoa. This is particularly the case for Cryptosporidium species (phylum Apicomplexa) which are difficult to propagate. Because of their polymorphic nature, simple sequence repeats have been used extensively as genotypic markers to differen...

journal_title：Parasitology

authors： Widmer G

更新日期：2018-08-01 00:00:00

abstract：:Eukaryotic 5S rRNA, synthesized by RNA polymerase III (Pol III), is an essential component of the large ribosomal subunit. Most organisms contain hundreds of 5S rRNA genes organized into tandem arrays. However, the genome of the protozoan parasite Leishmania major contains only 11 copies of the 5S rRNA gene, which are...

journal_title：Parasitology

authors： Moreno-Campos R,Florencio-Martínez LE,Nepomuceno-Mejía T,Rojas-Sánchez S,Vélez-Ramírez DE,Padilla-Mejía NE,Figueroa-Angulo E,Manning-Cela R,Martínez-Calvillo S

更新日期：2016-12-01 00:00:00

abstract：:Cats were repeatedly inoculated with infective larvae of Brugia pahangi. On parasitological grounds they could be divided into 5 groups. Group I--most cats (some 70%) became microfilaraemic (mf+) and retained high levels of microfilariae (mf) in their blood for over 2 years. In some Group I cats mf counts stabilized a...

journal_title：Parasitology

authors： Denham DA,Medeiros F,Baldwin C,Kumar H,Midwinter IC,Birch DW,Smail A

更新日期：1992-06-01 00:00:00

abstract：:Electrophoresis of secretions collected from Globodera pallida revealed a smeared region between 25 and 50 kDa, and a single band of < 20 kDa. The secretions were used to raise an antiserum (LW1). Immunoblotting of parasite homogenates with LW1 differentiated G. pallida from its sibling species G. rostochiensis and re...

journal_title：Parasitology

authors： Duncan LH,Robertson L,Robertson WM,Kusel JR

更新日期：1997-10-01 00:00:00

abstract：:A key issue relating to developing multi-component anti-malarial vaccines, lies in studying Plasmodium vivax surface proteins' genetic variation. The present work was aimed at amplifying, cloning and sequencing the gene encoding P. vivax merozoite surface protein 5 (PvMSP5) in samples obtained from infected patients f...

journal_title：Parasitology

authors： Gomez A,Suarez CF,Martinez P,Saravia C,Patarroyo MA

更新日期：2006-12-01 00:00:00

abstract：:We have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangi and Dirofilaria imitis) and of the free-living soil nematodes Caenorhabditis elegans and have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal dena...

journal_title：Parasitology

authors： Rothstein N,Stoller TJ,Rajan TV

更新日期：1988-08-01 00:00:00

abstract：:The use of parasites as biological tags to discriminate among marine fish stocks has become a widely accepted method in fisheries management. Here, we first link this approach to its unstated ecological foundation, the decay in the similarity of the species composition of assemblages as a function of increasing distan...

journal_title：Parasitology

authors： Poulin R,Kamiya T

更新日期：2015-01-01 00:00:00

abstract：:Freshwater snails of the Bulinus forskalii group are one of four Bulinus species complexes responsible for the transmission of schistosomes in Africa and adjacent regions. The species status of these conchologically variable and widely distributed planorbids remains unclear, and parasite compatibility varies considera...

journal_title：Parasitology

authors： Jones CS,Rollinson D,Mimpfoundi R,Ouma J,Kariuki HC,Noble LR

更新日期：2001-01-01 00:00:00

abstract：:Intraerythrocytic malaria parasites ingest the cytosol of their host cell and digest it inside their acid food vacuoles. Acidified (pH 4-5.5, 37 degrees C) human red blood cell lysates were used to simulate this process, measuring the denaturation of haemoglobin (Hb) and the release of iron, in the absence or presence...

journal_title：Parasitology

authors： Gabay T,Krugliak M,Shalmiev G,Ginsburg H

更新日期：1994-05-01 00:00:00

abstract：:In this review, we envisage the host environment, not as a hostile one, since the schistosome thrives there, but as one in which the relationship between the two organisms consists of constant communication, through signalling mechanisms involving sense organs, surface glycocalyx, surface membrane and internal organs ...

journal_title：Parasitology

authors： Kusel JR,Al-Adhami BH,Doenhoff MJ

更新日期：2007-10-01 00:00:00

abstract：:Treatment of BALB/c or MF1 mice with cyclosporin A (CsA) around the time of infection with Schistosoma mansoni conferred almost complete protection. The migration kinetics of L-[75Se]selenomethionine-labelled infective cercariae were investigated by compressed tissue autoradiography. Similar levels of skin penetration...

journal_title：Parasitology

authors： Munro GH,Brannan LR,Chappell LH,Thomson AW,McLaren DJ

更新日期：1991-02-01 00:00:00

abstract：:We investigated the segregation of an intracellular microsporidian parasite during host cell division. A time-course experiment was carried out to examine the distribution of parasites relative to host chromosomal DNA via light and electron microscopy. Fluorescent light microscopy and EM studies showed that the parasi...

journal_title：Parasitology

authors： Terry RS,Dunn AM,Smith JE

更新日期：1999-01-01 00:00:00

abstract：:The parasitic roundworms Strongyloides stercoralis (in man) and Strongyloides ratti (in rats) employ environmentally controlled XX/XO sex determination with a pair of X chromosomes and two pairs of autosomes. Strongyloides papillosus (in sheep) has only two pairs of chromosomes, one of which combines the genetic mater...

journal_title：Parasitology

authors： Kulkarni A,Dyka A,Nemetschke L,Grant WN,Streit A

更新日期：2013-12-01 00:00:00

abstract：:As has been shown previously, immunologically intact mice with patent Schistosoma mansoni infections had a significantly lower mean platelet number than intact uninfected mice (P<0.0001). However, platelet numbers in T-cell deprived mice with patent infections were not significantly different from those in uninfected ...

journal_title：Parasitology

authors： Stanley RG,Ngaiza JR,Atieno E,Jell G,Francklow K,Jackson CL,Parry H,Doenhoff MJ

更新日期：2003-03-01 00:00:00

abstract：:Avian malaria is a vector transmitted disease caused by Plasmodium and recent studies suggest that variation in its prevalence across avian hosts is correlated with a variety of ecological traits. Here we examine the relationship between prevalence and diversity of Plasmodium lineages in southeastern Amazonia and: (1)...

journal_title：Parasitology

authors： Fecchio A,Ellis VA,Bell JA,Andretti CB,D'Horta FM,Silva AM,Tkach VV,Weckstein JD

更新日期：2017-07-01 00:00:00

abstract：:To compare the structures of natural and re-established populations of Ascaris in humans, universal (mass) chemotherapy was carried out at the beginning and the end of the study year using pyrantel pamoate. Worms expelled within 48 h of treatment were collected, their sex determined, and measurements made of length, w...

journal_title：Parasitology

authors： Peng W,Cui X,Zhou X

更新日期：2002-06-01 00:00:00

abstract：:The response of the plant parasitic nematode, Meloidogyne incognita (J2 stage) to avermectin B2a-23-one is triphasic, comprising an initial loss of locomotor activity where the juveniles remain sensitive to touch, a recovery phase and a final loss of activity where the juveniles are relatively insensitive to touch. In...

journal_title：Parasitology

authors： Wright DJ,Birtle AJ,Roberts IT

更新日期：1984-04-01 00:00:00

abstract：:A cDNA encoding a beta-galactoside-binding lectin (galectin) was identified by immunoscreening a Haemonchus contortus cDNA library with antisera from lambs vaccinated with a membrane protein complex (H-gal-GP) derived from the parasites' gut. The cDNA sequence, exhibiting a tandem repeat structure and designated Hco-g...

journal_title：Parasitology

authors： Newlands GF,Skuce PJ,Knox DP,Smith SK,Smith WD

更新日期：1999-11-01 00:00:00

abstract：:Bloodstream forms of Trypanosoma congolense (2 clones: ILNat3.1 and IL3000, and 4 stocks: IL2079, IL2466, IL3266 and CP-81) were continuously cultivated in vitro at 34-36 degrees C in the absence of feeder cell layers, using HMI-93 medium which was modified from Iscove's modified Dulbecco's MEM (Flow Laboratories, Irv...

journal_title：Parasitology

authors： Hirumi H,Hirumi K

更新日期：1991-04-01 00:00:00

abstract：:Ultrastructural investigations (SEM, TEM) combined with lectin-binding analysis, have revealed concurrent modifications in tegumentary structure and surface glycoconjugates during the establishment and differentiation of Echinococcus multilocularis metacestodes in jirds. The laminated layer, which is amorphous and ric...

journal_title：Parasitology

authors： Leducq R,Gabrion C

更新日期：1992-02-01 00:00:00

abstract：:A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of ...

journal_title：Parasitology

authors： Lockyer MJ,Cooper H,Tite J,Rowan W,Crowe JS

更新日期：1993-06-01 00:00:00

abstract：:The effect of the parasitic swimbladder nematode, Anguillicola crassus, on the physiological status of wild European eels (Anguilla anguilla) was investigated during an 18 month survey (February 1995 until September 1996), and compared with that of wild uninfected eels collected over the same time-period. Despite the ...

journal_title：Parasitology

authors： Kelly CE,Kennedy CR,Brown JA

更新日期：2000-02-01 00:00:00

abstract：:Leishmania donovani causes visceral disease (kala-azar), a major health problem throughout the tropics with 500 000 new cases every year. Leishmania differentiates from the promastigote to the amastigote form to establish infection in a mammalian host. To understand the process of differentiation, we assessed the glob...

journal_title：Parasitology

authors： Srividya G,Duncan R,Sharma P,Raju BV,Nakhasi HL,Salotra P

更新日期：2007-10-01 00:00:00

abstract：:The Anaplasma species are important globally distributed tick-transmitted bacteria of veterinary and public health importance. These pathogens, cause anaplasmosis in domestic and wild animal species including humans. Rhipicephalus, Ixodes, Dermacentor and Amblyomma genera of ticks are the important vectors of Anaplasm...

journal_title：Parasitology

authors： Atif FA

更新日期：2016-05-01 00:00:00

abstract：:A new model for the study of granuloma formation in the liver is described. Rats received an injection of 20,000 Schistosoma mansoni eggs in the portal vein and granuloma formation was evaluated at 3, 5 and 7 weeks post-injection. Liver collagen was estimated at the same time and serum procollagen III peptide, a marke...

journal_title：Parasitology

authors： Van Gool J,Van Tiel D,Doenhoff MJ,Van Vugt H

更新日期：1991-02-01 00:00:00

abstract：:Theileria annulata infects and reversibly transforms bovine leucocytes. The parasite-transformed cells are immortalized, metastatic and express a number of metalloproteinases including matrix metalloproteinase 9 which they secrete. All the metalloproteinases observed on substrate gels are inhibited by tissue inhibitor...

journal_title：Parasitology

authors： Adamson RE,Hall FR

更新日期：1996-11-01 00:00:00

abstract：:Multiple parasitic infections have become increasingly recognized as a result of improvements in laboratory diagnosis and a growing population of immunocompromised individuals. This review examines the principles of chemotherapy in groupings of multiple infections which are of particular clinical significance. ...

journal_title：Parasitology

authors： Chiodini PL

更新日期：2001-01-01 00:00:00

abstract：:Biochemical characterization of 137 Leishmania braziliensis isolates from South and Central America, and from selected endemic foci in Bolivia, Brazil and Colombia, performed by isoenzymatic electrophoresis using 10 enzymatic systems, showed a high enzymatic polymorphism (44 zymodemes obtained) based on the variation ...

journal_title：Parasitology

authors： Chouicha N,Lanotte G,Pratlong F,Cuba Cuba CA,Velez ID,Dedet JP

更新日期：1997-10-01 00:00:00

abstract：:Susceptibility to oxidative stress is a well-established feature of the malarial parasite. Pharmacologists have taken advantage of this property to design highly effective pro-oxidant antimalarial drugs. Less well appreciated is the fact that nutritional manipulation of host oxidative stress status by dietary means ca...

journal_title：Parasitology

authors： Levander OA,Ager AL Jr

更新日期：1993-01-01 00:00:00

abstract：:While purine transport has been widely studied in protozoa, almost nothing is known about their capacity to salvage pyrimidines. Here, we report a Leishmania major transporter with high affinity for uracil (Km=0.32+/-0.07 microM) which we designated LmU1. This transporter displayed a high degree of specificity, as it ...

journal_title：Parasitology

authors： Papageorgiou IG,Yakob L,Al Salabi MI,Diallinas G,Soteriadou KP,De Koning HP

更新日期：2005-03-01 00:00:00