DNA base composition of filarial nematodes.

Abstract:

:We have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangi and Dirofilaria imitis) and of the free-living soil nematodes Caenorhabditis elegans and have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal denaturation and direct analysis of base content by HPLC following enzymatic hydrolysis, reveal that the GC content of filarial nematodes is 26-28%. We have been unable to find methylcytosine in the DNA of B. malayi.

journal_name

Parasitology

journal_title

Parasitology

authors

Rothstein N,Stoller TJ,Rajan TV

doi

10.1017/s0031182000066750

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

75-9

eissn

0031-1820

issn

1469-8161

journal_volume

97 ( Pt 1)

pub_type

杂志文章
  • Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences.

    abstract::The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000067263

    authors: Allsopp BA,Baylis HA,Allsopp MT,Cavalier-Smith T,Bishop RP,Carrington DM,Sohanpal B,Spooner P

    更新日期:1993-08-01 00:00:00

  • Trypanosomiasis and the brain.

    abstract::Neurological involvement following trypanosome infection has been recognised for over a century. However, there are still many unanswered questions concerning the mechanisms used by the parasite to gain entry to the CNS and the pathogenesis of the resulting neuroinflammatory reaction. There is a paucity of material fr...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182009991806

    authors: Rodgers J

    更新日期:2010-12-01 00:00:00

  • Repeated infection of cats with Brugia pahangi: parasitological observations.

    abstract::Cats were repeatedly inoculated with infective larvae of Brugia pahangi. On parasitological grounds they could be divided into 5 groups. Group I--most cats (some 70%) became microfilaraemic (mf+) and retained high levels of microfilariae (mf) in their blood for over 2 years. In some Group I cats mf counts stabilized a...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063666

    authors: Denham DA,Medeiros F,Baldwin C,Kumar H,Midwinter IC,Birch DW,Smail A

    更新日期:1992-06-01 00:00:00

  • Phylogenetic structure of host spectra in Palaearctic fleas: stability versus spatial variation in widespread, generalist species.

    abstract::We investigated spatial variation in the phylogenetic structure of host spectra in fleas parasitic on small mammals. Measures of phylogenetic host specificity ((phylogenetic species clustering (PSC) and phylogenetic species variability (PSV)) varied significantly more between than within flea species, but the proporti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182013001376

    authors: Krasnov BR,Pilosof S,Shenbrot GI,Khokhlova IS,Degen AA

    更新日期:2014-02-01 00:00:00

  • Interspecific characterization of several taeniid cestodes by isoenzyme analysis using isoelectric focusing in agarose.

    abstract::Taenia cestodes were obtained from 5 different definitive host species in Kenya and 175 different samples were examined by classical morphological methods and by isoenzyme analysis using isoelectric focusing in agarose. Gels were stained for 17 different enzymes and 3 of these were used in the construction of isoenzym...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000058017

    authors: Allsopp BA,Jones A,Allsopp MT,Newton SD,Macpherson CN

    更新日期:1987-12-01 00:00:00

  • Purification of a 64 kDa antigen from Trypanosoma evansi that exhibits cross-reactivity with Trypanosoma vivax.

    abstract::Trypanosoma evansi and Trypanosoma vivax are the most extensively distributed trypanosomes responsible for diseases in livestock. Western blot and indirect immunofluorescence assays revealed a high immunological cross-reaction between these two parasites. An antigen with an apparent molecular mass of 64 kDa (p64), whi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001001214

    authors: Uzcanga G,Mendoza M,Aso PM,Bubis J

    更新日期:2002-03-01 00:00:00

  • Cytochemical studies on nuclear DNA of four eucoccidian parasites, Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei.

    abstract::Feulgen-pararosaniline (SO2) staining was performed on stages in the life-cycle of Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei. The fluorescence emission of the stained DNA in nuclei of these stages was examined and compared with absorption microscopy measurements at 560 nm ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000054305

    authors: Cornelissen AW,Overdulve JP,Van der Ploeg M

    更新日期:1984-02-01 00:00:00

  • Future trends in diagnosis using laboratory-on-a-chip technologies.

    abstract::There has been an enormous growth in the development of biotechnological applications, where advances in the techniques of microelectronic fabrication and the technologies of miniaturization and integration in semiconductor industries are being applied to the production of Laboratory-on-a-Chip devices. The aim of this...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182099004126

    authors: Talary MS,Burt JP,Pethig R

    更新日期:1998-01-01 00:00:00

  • A panel of antigens of muscle larvae of Trichinella spiralis and T. pseudospiralis as revealed by two-dimensional western blot and immunoelectron microscopy.

    abstract::This study characterized antigens of Trichinella spiralis and T. pseudospiralis muscle larvae recognized by mice infected with the worms. Two-dimensional (2-D) Western blot analysis revealed some profile of antigenic peptides including: (1) molecular weight (MW); (2) isoelectric points (pI), (3) reactivity to well-def...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099004187

    authors: Wu Z,Nagano I,Takahashi Y

    更新日期:1999-06-01 00:00:00

  • Gill monogenean communities (Platyhelminthes, Monogenea, Dactylogyridae) of butterflyfishes from tropical Indo-West Pacific Islands.

    abstract::We studied the monogenean communities of 34 species of butterflyfish from the tropical Indo-West Pacific, identifying 13 dactylogyrid species (including two species that are presently undescribed). Monogenean assemblages differed significantly between host species in terms of taxonomic structure, intensity and prevale...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001463

    authors: Reverter M,Cutmore SC,Bray R,Cribb TH,Sasal P

    更新日期:2016-10-01 00:00:00

  • A new thrombospondin-related anonymous protein homologue in Neospora caninum (NcMIC2-like1).

    abstract::Neospora caninum is an Apicomplexan protozoan that has the dog as a definitive host and cattle (among other animals) as intermediate hosts. It causes encephalopathy in dogs and abortion in cows, with significant loss in worldwide livestock. As any Apicomplexan, the parasite invades the cells using proteins contained i...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010001290

    authors: Pereira LM,Candido-Silva JA,De Vries E,Yatsuda AP

    更新日期:2011-03-01 00:00:00

  • Synergistic antimalarial activity of ketones with rufigallol and vitamin C.

    abstract::Malaria remains a major cause of human morbidity and mortality worldwide. Plasmodium falciparum, the most virulent of the 4 human Plasmodium species causing malaria, is potentially life threatening, is increasing in prevalence and is becoming even more resistant to in-use drugs. In light of the growing problem of mult...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005008267

    authors: Mahajan SS,Kamath VR,Ghatpande SS

    更新日期:2005-10-01 00:00:00

  • A novel assay for the detection of anthelmintic activity mediated by cuticular damage to nematodes: validation on Caenorhabditis elegans exposed to cysteine proteinases.

    abstract::Plant cysteine proteinases (CPs) from Carica papaya kill parasitic and free-living nematodes in vitro by hydrolysis of the worm cuticle, a mechanism that is different to all commercially available synthetic anthelmintics. We have developed a cheap and effective, rapid-throughput Caenorhabditis elegans-based assay for ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016002353

    authors: Phiri AM,DE Pomerai DI,Buttle DJ,Behnke JM

    更新日期:2017-04-01 00:00:00

  • Axenic cultivation and partial characterization of Leishmania braziliensis amastigote-like stages.

    abstract::Leishmania braziliensis strain M2903 was adapted for growth and serially maintained as amastigotes at 34 degrees C in modified UM-54 medium, with growth curves exhibiting typical log and stationary phases. In late passages, amastigote growth took place in the absence of supplementary haemin and was unaffected when the...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118209700214x

    authors: Balanco JM,Pral EM,da Silva S,Bijovsky AT,Mortara RA,Alfieri SC

    更新日期:1998-02-01 00:00:00

  • Prior residency does not always pay off--co-infections in Daphnia.

    abstract::The epidemiological and ecological processes which govern the success of multiple-species co-infections are as yet unresolved. Here we investigated prior versus late residency within hosts, meaning which parasite contacts the host first, to determine if the outcomes of intra-host competition are altered. We infected a...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010000296

    authors: Lohr JN,Yin M,Wolinska J

    更新日期:2010-09-01 00:00:00

  • Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro.

    abstract::We examined the mechanism of action and compared the anthelmintic efficacy of cysteine proteinases from papaya, pineapple, fig, kiwi fruit and Egyptian milkweed in vitro using the rodent gastrointestinal nematode Heligmosomoides polygyrus. Within a 2 h incubation period, all the cysteine proteinases, with the exceptio...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004006225

    authors: Stepek G,Buttle DJ,Duce IR,Lowe A,Behnke JM

    更新日期:2005-02-01 00:00:00

  • Characterization of an atypical antigen from Sarcoptes scabiei containing an MADF domain.

    abstract::We have cloned a cDNA encoding a novel antigen from a Sarcoptes scabiei (Acari) cDNA library by immunoscreening with sera from S. scabiei-infected dogs. The antigen is encoded by a 2,157 bp mRNA with a predicted open reading frame of 719 amino acids (molecular weight 79 kDa). Our sequence analysis identified the prese...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005008747

    authors: Ljunggren EL,Bergström K,Morrison DA,Mattsson JG

    更新日期:2006-01-01 00:00:00

  • Dipstick dot ELISA for the detection of Taenia coproantigens in humans.

    abstract::A dipstick dot ELISA for detection of Taenia-specific coproantigens was developed. The test was based on a sandwich ELISA using antibodies raised against adult Taenia solium. Antibodies were absorbed to nitrocellulose paper previously adhered to acetate plastic to form dipsticks. Once blocked with 5% skimmed milk and ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000079439

    authors: Allan JC,Mencos F,Garcia-Noval J,Sarti E,Flisser A,Wang Y,Liu D,Craig PS

    更新日期:1993-07-01 00:00:00

  • Biochemical and ultrastructural investigation of the effect of Stelazine (trifluoperazine) on Hymenolepis diminuta (Cestoda).

    abstract::The effects of the phenothiazine, Stelazine, on Hymenolepis diminuta were investigated. The cestode was incubated for 10 min at 37 degrees C with 1 mM trifluoperazine, in the presence and absence of Ca2+. Assay of brush border enzymes showed that drug treatment lowered the activities of alkaline phosphatase, Ca2+-ATP'...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118200005352x

    authors: Hipkiss JB,Skinner A,Branford White CJ

    更新日期:1987-02-01 00:00:00

  • Epidemiological investigation of a severe rumen fluke outbreak on an Irish dairy farm.

    abstract::Although the rumen fluke, Calicophoron daubneyi is now very common and widespread throughout Western Europe, reports of clinical cases are still rare. This study explores the epidemiological background to a severe rumen fluke outbreak in 6-month-old heifers on a dairy farm in Ireland. Sequence analysis of the cytochro...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017002086

    authors: O'Shaughnessy J,Garcia-Campos A,McAloon CG,Fagan S,de Waal T,McElroy M,Casey M,Good B,Mulcahy G,Fagan J,Murphy D,Zintl A

    更新日期:2018-06-01 00:00:00

  • Features of amino acid metabolism in Moniliformis moniliformis (Acanthocephala) in vitro.

    abstract::Experiments to investigate the metabolism of glycine, L-glutamic acid and L-aspartic acid by Moniliformis moniliformis were carried out by incubating adult worms aerobically for 3 h at 37 degrees C in Tyrode's solution containing either [U-14C]glycine, L-[U-14C]glutamic acid, L-[U-14C]aspartic acid or L-[4-14C]asparti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055876

    authors: Ward PF,Crompton DW

    更新日期:1987-06-01 00:00:00

  • Eimeria tenella, E. acervulina and E. maxima: studies on the development of resistance to diclazuril and other anticoccidial drugs in the chicken.

    abstract::Resistance to diclazuril was induced by 10 passages of the Houghton strains of Eimeria acervulina and E. tenella in chickens given progressively greater concentrations of the drug. This resistance was, however, not complete since the drug retained some efficacy against the drug-passaged lines. Attempts to passage the ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000058625

    authors: Chapman HD

    更新日期:1989-10-01 00:00:00

  • Immunomodulatory agents in the laboratory and clinic.

    abstract::This paper reviews naturally occurring and synthetic compounds that either enhance immune defences or lower both natural and acquired immunity. Immunomodulatory agents used both for laboratory study and clinically for the management of immunologically based diseases are considered. ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182000075326

    authors: Nüssler AK,Thomson AW

    更新日期:1992-01-01 00:00:00

  • Purification and characterization of a saccharide-binding protein from penetration glands of Diplostomum pseudospathaceum--a bifunctional molecule with cysteine protease activity.

    abstract::A beta-1,3-glucan-binding lectin from the penetration glands of Diplostomum pseudospathaceum cercariae was isolated by affinity chromatography using yeast glucan and curdlan as affinity matrices. Further purification to homogeneity was performed by cation-exchange chromatography. The protein migrated as a double band ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182003003305

    authors: Mikes L,Man P

    更新日期:2003-07-01 00:00:00

  • Attraction behaviour of three entomopathogenic nematode species towards infected and uninfected hosts.

    abstract::Entomopathogenic nematode infective juveniles are likely to encounter both uninfected and infected insects and host quality depends on the stage of the infection. We hypothesized that nematode response to infected hosts will change over the course of an infection. Here, we tested this hypothesis by focusing on the inf...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006001880

    authors: Ramos-Rodríguez O,Campbell JF,Christen JM,Shapiro-Ilan DI,Lewis EE,Ramaswamy SB

    更新日期:2007-05-01 00:00:00

  • Parastrongyloides trichosuri suggests that XX/XO sex determination is ancestral in Strongyloididae (Nematoda).

    abstract::The parasitic roundworms Strongyloides stercoralis (in man) and Strongyloides ratti (in rats) employ environmentally controlled XX/XO sex determination with a pair of X chromosomes and two pairs of autosomes. Strongyloides papillosus (in sheep) has only two pairs of chromosomes, one of which combines the genetic mater...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182013001315

    authors: Kulkarni A,Dyka A,Nemetschke L,Grant WN,Streit A

    更新日期:2013-12-01 00:00:00

  • Marine parasites as biological tags in South American Atlantic waters, current status and perspectives.

    abstract::Many marine fisheries in South American Atlantic coasts (SAAC) are threatened by overfishing and under serious risk of collapsing. The SAAC comprises a diversity of environments, possesses a complex oceanography and harbours a vast biodiversity that provide an enormous potential for using parasites as biological tags ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182013002138

    authors: Cantatore DM,Timi JT

    更新日期:2015-01-01 00:00:00

  • The virus infecting the parasitoid Leptopilina boulardi exerts a specific action on superparasitism behaviour.

    abstract::Parasites often induce behavioural changes in their host. However, it is not necessarily easy to determine whether these changes are representative of an adaptation of the parasite (parasite manipulation), an adaptive response of the host or a side-effect of infection. In a solitary parasitoid of Drosophila larvae (Le...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006009930

    authors: Varaldi J,Petit S,Boulétreau M,Fleury F

    更新日期:2006-06-01 00:00:00

  • Subtype analysis of Cryptosporidium parvum isolates from calves on farms around Belgrade, Serbia and Montenegro, using the 60 kDa glycoprotein gene sequences.

    abstract::Cryptosporidium parvum is composed of multiple subtypes, which appear to be zoonotic or anthroponotic. Therefore, in order to understand the molecular epidemiology of this species and to control the zoonotic transmission, it is essential to identify the isolates at the subtype level. In the present study, 60.2% of 103...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006001508

    authors: Misic Z,Abe N

    更新日期:2007-03-01 00:00:00

  • Individual and combined effects of cadmium and 3,3',4,4',5-pentachlorobiphenyl (PCB 126) on the humoral immune response in European eel (Anguilla anguilla) experimentally infected with larvae of Anguillicola crassus (Nematoda).

    abstract::The individual and combined effects of cadmium (Cd) and 3,3',4,4',5-pentachlorobiphenyl (PCB 126) on the antibody response of fish against metazoan parasites were tested. Eels experimentally infected with the swim bladder nematode Anguillicola crassus were exposed to sublethal concentrations of Cd and PCB 126. Cd was ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118200300475x

    authors: Sures B,Knopf K

    更新日期:2004-04-01 00:00:00