Purification of a 64 kDa antigen from Trypanosoma evansi that exhibits cross-reactivity with Trypanosoma vivax.

Abstract:

:Trypanosoma evansi and Trypanosoma vivax are the most extensively distributed trypanosomes responsible for diseases in livestock. Western blot and indirect immunofluorescence assays revealed a high immunological cross-reaction between these two parasites. An antigen with an apparent molecular mass of 64 kDa (p64), which exhibited cross-reactivity with T. vivax, was purified to homogeneity from a Venezuelan isolate of T. evansi. This antigen is glycosylated, contains a glycosyl-phosphatidylinositol anchor and appeared to be localized through the cell except in the nucleus, indicating that it could primarily be confined to the parasite surface. These results, together with its relative abundance and apparent molecular weight, suggest that p64 probably corresponds to the soluble form of a variable surface glycoprotein from T. evansi. Anti-p64 polyclonal antibodies, raised on mice, recognized a 53 kDa polypeptide band from a Venezuelan isolate of T. vivax on Western blots. Additionally, sera obtained from naturally infected animals also recognized p64, suggesting its potential use as a diagnostic reagent. Mild acid treatment only slightly decreased the immunorecognition of p64, suggesting its potential use as a diagnostic reagent. Mild acid treatment only slightly decreased the immunorecognition of p64, demonstrating that another relevant cross-reacting epitope, different than the inositol-1,2-cyclic phosphate of the cross-reacting determinant, must exist in p64. To date, p64 represents the first antigen isolated and partially characterized from T. evansi.

journal_name

Parasitology

journal_title

Parasitology

authors

Uzcanga G,Mendoza M,Aso PM,Bubis J

doi

10.1017/s0031182001001214

subject

Has Abstract

pub_date

2002-03-01 00:00:00

pages

287-99

issue

Pt 3

eissn

0031-1820

issn

1469-8161

journal_volume

124

pub_type

杂志文章
  • Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study.

    abstract::The recent development of genetic methods allows the delineation of species boundaries, especially in organisms where morphological characters are not reliable to differentiate species. However, few empirical studies have used these tools to delineate species among parasitic metazoans. Here we investigate the species ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001517

    authors: Pérez-Ponce DE León G,García-Varela M,Pinacho-Pinacho CD,Sereno-Uribe AL,Poulin R

    更新日期:2016-11-01 00:00:00

  • Avian malaria, ecological host traits and mosquito abundance in southeastern Amazonia.

    abstract::Avian malaria is a vector transmitted disease caused by Plasmodium and recent studies suggest that variation in its prevalence across avian hosts is correlated with a variety of ecological traits. Here we examine the relationship between prevalence and diversity of Plasmodium lineages in southeastern Amazonia and: (1)...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201700035X

    authors: Fecchio A,Ellis VA,Bell JA,Andretti CB,D'Horta FM,Silva AM,Tkach VV,Weckstein JD

    更新日期:2017-07-01 00:00:00

  • The effect of temperature on times to hatching of eggs of the nematode Ostertagia circumcincta.

    abstract::A delayed gamma distribution satisfactorily described the distribution of times to hatching of Ostertagia circumcincta eggs incubated in 0.1% saline at temperatures between 6 and 20 degrees C. Below 6 degree C hatching of eggs was extremely variable. The relationship between rates of development and temperature within...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000061874

    authors: Young RR,Anderson N,Overend D,Tweedie RL,Malafant KW,Preston GA

    更新日期:1980-12-01 00:00:00

  • Costs of resistance in insect-parasite and insect-parasitoid interactions.

    abstract::Most, if not all, organisms face attack by natural enemies and will be selected to evolve some form of defence. Resistance may have costs as well as its obvious benefits. These costs may be associated with actual defence or with the maintenance of the defensive machinery irrespective of whether a challenge occurs. In ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182002001750

    authors: Kraaijeveld AR,Ferrari J,Godfray HC

    更新日期:2002-01-01 00:00:00

  • Identification of a developmentally regulated Schistosoma mansoni serine protease homologous to mouse plasma kallikrein and human factor I.

    abstract::The isolation of 2 genomic clones has allowed us to further characterize a Schistosoma mansoni serine protease designated SmSP1. The deduced amino acid sequence (248aa) considered as a 'light chain' encoding the active site, presents significant homologies with mouse plasma kallikrein and human factor I light chain. T...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182098003874

    authors: Cocude C,Pierrot C,Cêtre C,Fontaine J,Godin C,Capron A,Khalife J

    更新日期:1999-04-01 00:00:00

  • Features of amino acid metabolism in Moniliformis moniliformis (Acanthocephala) in vitro.

    abstract::Experiments to investigate the metabolism of glycine, L-glutamic acid and L-aspartic acid by Moniliformis moniliformis were carried out by incubating adult worms aerobically for 3 h at 37 degrees C in Tyrode's solution containing either [U-14C]glycine, L-[U-14C]glutamic acid, L-[U-14C]aspartic acid or L-[4-14C]asparti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055876

    authors: Ward PF,Crompton DW

    更新日期:1987-06-01 00:00:00

  • Distinctive histopathology and modulation of cytokine production during oral and intraperitoneal Trypanosoma cruzi Y strain infection.

    abstract::Acute Chagas disease outbreaks are related to the consumption of food or drink contaminated by triatomine feces, thus making oral infection an important route of transmission. Both vector-borne and oral infections trigger important cardiac manifestations in the host that are related to a dysregulated immune response. ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182013002059

    authors: Kuehn CC,Oliveira LG,Miranda MA,Prado JC

    更新日期:2014-06-01 00:00:00

  • Phylogeny of snake trypanosomes inferred by SSU rDNA sequences, their possible transmission by phlebotomines, and taxonomic appraisal by molecular, cross-infection and morphological analysis.

    abstract::Blood examination by microhaematocrit and haemoculture of 459 snakes belonging to 37 species revealed 2.4% trypanosome prevalence in species of Viperidae (Crotalus durissus and Bothrops jararaca) and Colubridae (Pseudoboa nigra). Trypanosome cultures from C. durissus and P. nigra were behaviourally and morphologically...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008004253

    authors: Viola LB,Campaner M,Takata CS,Ferreira RC,Rodrigues AC,Freitas RA,Duarte MR,Grego KF,Barrett TV,Camargo EP,Teixeira MM

    更新日期:2008-04-01 00:00:00

  • Time of treatment influences the appearance of drug-resistant parasites in Plasmodium falciparum infections.

    abstract::A deterministic mathematical model which predicts the probability of developing a new drug-resistant parasite population within the human host is reported. The model incorporates the host's specific antibody response to PfEMP1, and also investigates the influence of chemotherapy on the probability of developing a viab...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001008824

    authors: Gatton ML,Hogarth W,Saul A

    更新日期:2001-12-01 00:00:00

  • Bank voles (Myodes glareolus) and house mice (Mus musculus musculus; M. m. domesticus) in Europe are each parasitized by their own distinct species of Aspiculuris (Nematoda, Oxyurida).

    abstract::The molecular phylogeny and morphology of the oxyuroid nematode genus Aspiculuris from voles and house mice has been examined. Worms collected from Myodes glareolus in Poland, Eire and the UK are identified as Aspiculuris tianjinensis, previously known only from China, while worms from Mus musculus from a range of loc...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182015000864

    authors: Behnke JM,Stewart A,Bajer A,Grzybek M,Harris PD,Lowe A,Ribas A,Smales L,Vandegrift KJ

    更新日期:2015-10-01 00:00:00

  • Density-dependent processes in the transmission of human onchocerciasis: intensity of microfilariae in the skin and their uptake by the simuliid host.

    abstract::The transmission success of Onchocerca volvulus is thought to be influenced by a variety of regulatory or density-dependent processes that act at various points in the two-host life-cycle. This paper examines one component of the life-cycle, namely, the ingestion of microfilariae by the simuliid vector, to assess the ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000078586

    authors: Basáñez MG,Boussinesq M,Prod'hon J,Frontado H,Villamizar NJ,Medley GF,Anderson RM

    更新日期:1994-01-01 00:00:00

  • The specificity of purine base and nucleoside uptake in promastigotes of Leishmania braziliensis panamensis.

    abstract::Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported a...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055256

    authors: Hansen BD,Perez-Arbelo J,Walkony JF,Hendricks LD

    更新日期:1982-10-01 00:00:00

  • Identification of a Nosema bombycis (Microsporidia) spore wall protein corresponding to spore phagocytosis.

    abstract::Life-cycle stages of the microsporidia Nosema bombycis, the pathogen causing silkworm pebrine, were separated and purified by an improved method of Percoll-gradient centrifugation. Soluble protein fractions of late sporoblasts (spore precursor cells) and mature spores were analysed by two-dimensional polyacrylamide ge...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011000801

    authors: Cai S,Lu X,Qiu H,Li M,Feng Z

    更新日期:2011-08-01 00:00:00

  • Alpha 2 giardin is an assemblage A-specific protein of human infective Giardia duodenalis.

    abstract::Of the 7 genetic assemblages of the parasite Giardia duodenalis only 2 (A and B) are known to cause infections in humans. These assemblages have been characterized in detail at the genomic level but few studies have examined differences in the proteins expressed. Employing one and two-dimensional PAGE we have identifi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008004988

    authors: Steuart RF,O'Handley R,Lipscombe RJ,Lock RA,Thompson RC

    更新日期:2008-12-01 00:00:00

  • Trypanosomiasis and the brain.

    abstract::Neurological involvement following trypanosome infection has been recognised for over a century. However, there are still many unanswered questions concerning the mechanisms used by the parasite to gain entry to the CNS and the pathogenesis of the resulting neuroinflammatory reaction. There is a paucity of material fr...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182009991806

    authors: Rodgers J

    更新日期:2010-12-01 00:00:00

  • The development of Haemonchus contortus, a nematode parasite of the ovine abomasum, in the laboratory rabbit.

    abstract::The development and growth of Haemonchus contortus was studied in the stomach of the laboratory rabbit. Less than 1% of the ensheathed infective larvae given to rabbits became established, whereas after exsheathment in vitro under physiological conditions, 20-30% of the larvae administered developed to the 4th stage. ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000047867

    authors: Mapes CJ,Gallie GJ

    更新日期:1977-06-01 00:00:00

  • Heligmosomoides polygyrus (Nematoda): susceptible and resistant strains of mice are indistinguishable following natural infection.

    abstract::BALB/c mice were characterized as more resistant to infection with Heligmosomoides polygyrus (Nematoda) than C57BL/6 mice based on lower establishment and survival during a primary infection and stronger protection induced by an immunizing regime. It was hypothesized, therefore, that C57BL/6 mice would be more heavily...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000059953

    authors: Scott ME

    更新日期:1991-12-01 00:00:00

  • Signatures of mito-nuclear discordance in Schistosoma turkestanicum indicate a complex evolutionary history of emergence in Europe.

    abstract::High levels of molecular diversity were identified in mitochondrial cytochrome c oxidase (cox1) gene sequences of Schistosoma turkestanicum from Hungary. These cox1 sequences were all specific to Hungary which contrasted with the low levels of diversity seen in the nuclear internal transcribed spacer region (ITS) sequ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017000920

    authors: Lawton SP,Bowen LI,Emery AM,Majoros G

    更新日期:2017-11-01 00:00:00

  • The virus infecting the parasitoid Leptopilina boulardi exerts a specific action on superparasitism behaviour.

    abstract::Parasites often induce behavioural changes in their host. However, it is not necessarily easy to determine whether these changes are representative of an adaptation of the parasite (parasite manipulation), an adaptive response of the host or a side-effect of infection. In a solitary parasitoid of Drosophila larvae (Le...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006009930

    authors: Varaldi J,Petit S,Boulétreau M,Fleury F

    更新日期:2006-06-01 00:00:00

  • A comparison of the surface and secretions of Trichinella pseudospiralis and T. spiralis.

    abstract::Intact, viable adults, infective and newborn larvae of Trichinella pseudospiralis were surface labelled with 125I by the chloramine T method and labelled proteins were compared with those obtained from equivalent stages of T. spiralis. Electron-microscope autoradiography determined that labelled proteins were restrict...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118200004991x

    authors: Almond NM,McLaren DJ,Parkhouse RM

    更新日期:1986-08-01 00:00:00

  • The public health importance of hookworm disease.

    abstract::The public health significance of hookworm disease is briefly reviewed. The latest evidence confirms that blood loss caused by the worms' feeding activity in the gut is a contributing factor in the development of poor iron status leading to iron-deficiency anaemia. The World Health Organization has identified adolesce...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182000006454

    authors: Crompton DW

    更新日期:2000-01-01 00:00:00

  • Infection success of different trematode genotypes in two alternative intermediate hosts: evidence for intraspecific specialization?

    abstract::The evolution of host specificity and the potential trade-off between being a generalist and a specialist are central issues in the evolutionary ecology of parasites. Different species of parasites or even different populations of the same species often show different degrees of host specificity. However, less is know...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009991107

    authors: Leung TL,Poulin R

    更新日期:2010-02-01 00:00:00

  • Establishing mono-eukaryotic Histomonas meleagridis cultures from in vivo infection contaminated with Tetratrichomonas gallinarum and Blastocystis spp.

    abstract::SUMMARY The necessity to easily establish Histomonas meleagridis cultures has been underlined extensively by many researchers in order to gain more insights in the biology of H. meleagridis. In addition the occurrence of different protozoa in the caeca of birds impedes, however, the isolation and propagation of H. mel...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182013000723

    authors: Pham AD,Mast J,DE Gussem JK,McDougald LR,Goddeeris BM

    更新日期:2013-09-01 00:00:00

  • A second peak of egg excretion in Strongyloides ratti-infected rats: its origin and biological meaning.

    abstract::In Strongyloides ratti-infected rats, 2 peaks of egg excretion were observed; a large one with maximum egg production on days 7-8 of infection and a small more inconspicuous one around day 25. The second peak, which had been ignored in most studies, was produced by adults in the caecum and the colon. The adults were l...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099004631

    authors: Kimura E,Shintoku Y,Kadosaka T,Fujiwara M,Kondo S,Itoh M

    更新日期:1999-08-01 00:00:00

  • Isolation, fractionation and partial characterization of the tegumental surface from protoscoleces of the hydatid organism, Echinococcus granulosus.

    abstract::Several approaches were adopted for the disruption and removal of the tegumental surface from protoscoleces of the horse strain of the hydatid organism, Echinococcus granulosus. The effectiveness of each method and the purity of subsequent microthrix-enriched fractions obtained by differential centrifugation were eval...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000049064

    authors: McManus DP,Barrett NJ

    更新日期:1985-02-01 00:00:00

  • Vaccines for amoebiasis: barriers and opportunities.

    abstract::Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. While improved sanitation could lead to the eradication of this disease, it is unlikely that this will occur worldwide in the foreseeable future; thus alternative mea...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S003118200600182X

    authors: Stanley SL Jr

    更新日期:2006-01-01 00:00:00

  • DNA base composition of filarial nematodes.

    abstract::We have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangi and Dirofilaria imitis) and of the free-living soil nematodes Caenorhabditis elegans and have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal dena...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000066750

    authors: Rothstein N,Stoller TJ,Rajan TV

    更新日期:1988-08-01 00:00:00

  • Screening and synthesis: high throughput technologies applied to parasitology.

    abstract::High throughput technologies continue to develop in response to the challenges set by the genome projects. This article discusses how the techniques of both high throughput screening (HTS) and synthesis can influence research in parasitology. Examples of the use of targeted and phenotype-based HTS using unbiased compo...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182004007073

    authors: Morgan RE,Westwood NJ

    更新日期:2004-01-01 00:00:00

  • Tight binding between a pool of the heterodimeric alpha/beta tubulin and a protein kinase CK2 in Trypanosoma cruzi epimastigotes.

    abstract::Tubulin is the predominant phosphoprotein in Trypanosoma cruzi epimastigotes and is phosphorylated by a protein kinase CK2. Interestingly, the presence or absence of divalent cations affected the solubilization of a pool of the parasite tubulin and the CK2 responsible for its phosphorylation. This fraction of tubulin ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005009352

    authors: De Lima AR,Medina R,Uzcanga GL,Noris Suárez K,Contreras VT,Navarro MC,Arteaga R,Bubis J

    更新日期:2006-04-01 00:00:00

  • Natural Lyme disease cycles maintained via sheep by co-feeding ticks.

    abstract::We present observational and experimental evidence that cycles of the Lyme disease spirochaete, Borrelia burgdorferi s.l., can be maintained by sheep in the virtual absence of alternative hosts. A 2-year field study in upland moorland habitats of northwest UK established that sheep feed up to 80% of larval, > 99% of n...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182097001868

    authors: Ogden NH,Nuttall PA,Randolph SE

    更新日期:1997-12-01 00:00:00