Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences.

Abstract:

:The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central (variable) region of the srRNA genes of T. annulata, T. taurotragi, T. mutants and two unidentified parasites referred to as Theileria sp. (buffalo) and Theileria sp. (Marula) were obtained. An alignment of the sequences was generated from which 6 oligonucleotide probes, corresponding to species-specific regions, were designed. These probes were demonstrated to provide unequivocal identification of each of the 6 species either by direct detection of parasite srRNA or by hybridization to amplified parasite srRNA genes. The probes were not able to distinguish buffalo-derived T. parva, the causal agent of Corridor disease, from cattle-derived T. parva, the causal agent of East Coast fever.

journal_name

Parasitology

journal_title

Parasitology

authors

Allsopp BA,Baylis HA,Allsopp MT,Cavalier-Smith T,Bishop RP,Carrington DM,Sohanpal B,Spooner P

doi

10.1017/s0031182000067263

subject

Has Abstract

pub_date

1993-08-01 00:00:00

pages

157-65

eissn

0031-1820

issn

1469-8161

journal_volume

107 ( Pt 2)

pub_type

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