In vitro isolation of Plasmodium knowlesi merozoites using polycarbonate sieves.

Abstract:

:A culture chamber fitted with a polycarbonate sieve has been used to isolate Plasmodium knowlesi merozoites as they are released from schizonts. A 3 mum pore-size sieve allows passage of normal erythrocytes and red cells containing rings and trophozoites and can be used to concentrate schizonts from a mixed cell population. A 2 mum pore-size sieve retains normal and parasitized cells and provides uncontaminated merozoites in high yield (5 x 10(10) merozoites per ml schizonts). Merozoite viability diminishes rapidly during 30 min after isolation. These preparations should prove valuable for studies of the biochemical, physiological and antigenic properties of this transient phase of the malaria parasite.

journal_name

Parasitology

journal_title

Parasitology

authors

Dennis ED,Mitchell GH,Butcher GA,Cohen S

doi

10.1017/s0031182000047235

subject

Has Abstract

pub_date

1975-12-01 00:00:00

pages

475-81

issue

3

eissn

0031-1820

issn

1469-8161

journal_volume

71

pub_type

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