Abstract:
:A culture chamber fitted with a polycarbonate sieve has been used to isolate Plasmodium knowlesi merozoites as they are released from schizonts. A 3 mum pore-size sieve allows passage of normal erythrocytes and red cells containing rings and trophozoites and can be used to concentrate schizonts from a mixed cell population. A 2 mum pore-size sieve retains normal and parasitized cells and provides uncontaminated merozoites in high yield (5 x 10(10) merozoites per ml schizonts). Merozoite viability diminishes rapidly during 30 min after isolation. These preparations should prove valuable for studies of the biochemical, physiological and antigenic properties of this transient phase of the malaria parasite.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Dennis ED,Mitchell GH,Butcher GA,Cohen Sdoi
10.1017/s0031182000047235subject
Has Abstractpub_date
1975-12-01 00:00:00pages
475-81issue
3eissn
0031-1820issn
1469-8161journal_volume
71pub_type
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