Fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae of Ascaris suum.

Abstract:

:Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process of Ascaris suum larvae. This study describes moulting and development-specific protein expression profiles of A. suum lung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepared for protein analysis using two-dimensional (2D) electrophoresis. NaF exposure inhibited at least 22 proteins in AsLL3 compared with moulted larvae (i.e. AsLL4). A further comparison of AsLL4 with those of pre-cultured AsLL3 and NaF-exposed AsLL3 revealed 8 stage-specifically and 4 over-expressed proteins. Immunoblot analysis revealed an inhibition by NaF of 19 immunoreactive proteins. Enzyme assay and immunochemical data showed an inhibition of the moulting-specific inorganic pyrophosphatase activity by 41% and a decreased expression in NaF-treated larvae, indicating its significance in the moulting process. A protein spot associated with NaF inhibition was isolated and identified by peptide mass spectrometry and bioinformatics approaches to be a member of 3-hydroxyacyl-CoA dehydrogenase/short-chain dehydrogenase enzyme families. These results have implications for the identification of proteins specific to the moulting process as potential chemotherapeutic targets.

journal_name

Parasitology

journal_title

Parasitology

authors

Islam MK,Miyoshi T,Yamada M,Alim MA,Huang X,Motobu M,Tsuji N

doi

10.1017/S0031182006000576

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

497-508

issue

Pt 4

eissn

0031-1820

issn

1469-8161

pii

S0031182006000576

journal_volume

133

pub_type

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