A novel assay for the detection of anthelmintic activity mediated by cuticular damage to nematodes: validation on Caenorhabditis elegans exposed to cysteine proteinases.


:Plant cysteine proteinases (CPs) from Carica papaya kill parasitic and free-living nematodes in vitro by hydrolysis of the worm cuticle, a mechanism that is different to all commercially available synthetic anthelmintics. We have developed a cheap and effective, rapid-throughput Caenorhabditis elegans-based assay for screening plant CP extracts for anthelmintic activity targeting cuticular integrity. The assay exploits colorimetric methodology for assessment of cuticular damage, and is based on the ability of viable cells to incorporate and bind Neutral red dye within lysosomes and to release the dye when damaged. Living worms are pre-stained with the dye, exposed to CPs and then leakage of the dye through the damaged cuticle is quantified by spectrophotometry. In contrast to motility assays and semi-subjective interpretation of microscopical images, this colorimetric assay is independent of observer bias. Our assay was applied to a series of C. elegans bus mutant strains with leaky cuticles and to cystatin knockout mutants. At ambient temperature and over 0.5-24 h, both bus mutants and the cystatin knockouts were highly susceptible to CPs, whereas wild-type Bristol N2 worms were essentially unstained by Neutral red and unaffected by CPs, providing validation for the utility of this assay.






Phiri AM,DE Pomerai DI,Buttle DJ,Behnke JM




Has Abstract


2017-04-01 00:00:00














  • Physiological cost induced by the maternally-transmitted endosymbiont Wolbachia in the Drosophila parasitoid Leptopilina heterotoma.

    abstract::Endosymbiotic bacteria of the genus Wolbachia infect a number of invertebrate species in which they induce various alterations in host reproduction, mainly cytoplasmic incompatibility (CI). In contrast to most other maternally transmitted parasites, manipulation of host reproduction makes the spread of Wolbachia possi...


    pub_type: 杂志文章


    authors: Fleury F,Vavre F,Ris N,Fouillet P,Boulétreau M

    更新日期:2000-11-01 00:00:00

  • Proteomic analysis of a 120 kDa protein complex in cyst fluid of Taenia solium metacestode and preliminary evaluation of its value for the serodiagnosis of neurocysticercosis.

    abstract::Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of p...


    pub_type: 杂志文章


    authors: Lee EG,Bae YA,Jeong YT,Chung JY,Je EY,Kim SH,Na BK,Ju JW,Kim TS,Ma L,Cho SY,Kong Y

    更新日期:2005-12-01 00:00:00

  • Alpha 2 giardin is an assemblage A-specific protein of human infective Giardia duodenalis.

    abstract::Of the 7 genetic assemblages of the parasite Giardia duodenalis only 2 (A and B) are known to cause infections in humans. These assemblages have been characterized in detail at the genomic level but few studies have examined differences in the proteins expressed. Employing one and two-dimensional PAGE we have identifi...


    pub_type: 杂志文章


    authors: Steuart RF,O'Handley R,Lipscombe RJ,Lock RA,Thompson RC

    更新日期:2008-12-01 00:00:00

  • Cytogenetic and sequence comparison of adult Phyllodistomum (Digenea: Gorgoderidae) from the three-spined stickleback with larvae from two bivalves.

    abstract::Due to the low informative value of available morphological characters, cytogenetic and molecular methods, based on rDNA sequencing, were used to characterize adult and larval stages of Phyllodistomum spp. Species studied have 18 chromosomes with comparable absolute and relative lengths. Conventional Giemsa staining a...


    pub_type: 杂志文章


    authors: Petkeviciŭte R,Stunzenas V,Staneviciŭte G

    更新日期:2004-12-01 00:00:00

  • Rates and intensity of re-infection with human helminths after treatment and the influence of individual, household, and environmental factors in a Brazilian community.

    abstract::This study quantifies the rate and intensity of re-infection with human hookworm and Schistosoma mansoni infection 12 months following successful treatment, and investigates the influence of socio-economic, geographical and environmental factors. A longitudinal study of 642 individuals aged over 5 years was conducted ...


    pub_type: 杂志文章


    authors: Cundill B,Alexander N,Bethony JM,Diemert D,Pullan RL,Brooker S

    更新日期:2011-09-01 00:00:00

  • Diversity and distribution of Blastocystis sp. subtypes in non-human primates.

    abstract::Blastocystis SSU-rDNA sequence data from 317 captive and free-living non-human primates (NHPs) representing 30 genera of apes, Old and New World (OW and NW) monkeys and prosimians were analysed to investigate subtype (ST) and allele distribution among hosts. Excluding 20 mixed ST infections, 27% of the sequences belon...


    pub_type: 杂志文章


    authors: Alfellani MA,Jacob AS,Perea NO,Krecek RC,Taner-Mulla D,Verweij JJ,Levecke B,Tannich E,Clark CG,Stensvold CR

    更新日期:2013-07-01 00:00:00

  • Relative host body condition and food availability influence epidemic dynamics: a Poecilia reticulata-Gyrodactylus turnbulli host-parasite model.

    abstract::Understanding disease transmission is important to species management and human health. Host body condition, nutrition and disease susceptibility interact in a complex manner, and while the individual effects of these variables are well known, our understanding of how they interact and translate to population dynamics...


    pub_type: 杂志文章


    authors: Tadiri CP,Dargent F,Scott ME

    更新日期:2013-03-01 00:00:00

  • Segregation of a microsporidian parasite during host cell mitosis.

    abstract::We investigated the segregation of an intracellular microsporidian parasite during host cell division. A time-course experiment was carried out to examine the distribution of parasites relative to host chromosomal DNA via light and electron microscopy. Fluorescent light microscopy and EM studies showed that the parasi...


    pub_type: 杂志文章


    authors: Terry RS,Dunn AM,Smith JE

    更新日期:1999-01-01 00:00:00

  • Population dynamics in echinococcosis and cysticercosis: regulation of Taenia hydatigena and T. ovis in lambs through passively transferred immunity.

    abstract::A comparison has been made of the interactions between passively transferred and actively acquired immunity in regulating populations of Taenia hydatigena and T. ovis. When ewes were grazed prior to parturition under a high infection pressure, immunity was transferred to their offspring for up to 8 weeks. A qualitativ...


    pub_type: 杂志文章


    authors: Gemmell MA,Lawson JR,Roberts MG,Griffin JF

    更新日期:1990-08-01 00:00:00

  • Axenic cultivation and partial characterization of Leishmania braziliensis amastigote-like stages.

    abstract::Leishmania braziliensis strain M2903 was adapted for growth and serially maintained as amastigotes at 34 degrees C in modified UM-54 medium, with growth curves exhibiting typical log and stationary phases. In late passages, amastigote growth took place in the absence of supplementary haemin and was unaffected when the...


    pub_type: 杂志文章


    authors: Balanco JM,Pral EM,da Silva S,Bijovsky AT,Mortara RA,Alfieri SC

    更新日期:1998-02-01 00:00:00

  • Purification and characterization of a saccharide-binding protein from penetration glands of Diplostomum pseudospathaceum--a bifunctional molecule with cysteine protease activity.

    abstract::A beta-1,3-glucan-binding lectin from the penetration glands of Diplostomum pseudospathaceum cercariae was isolated by affinity chromatography using yeast glucan and curdlan as affinity matrices. Further purification to homogeneity was performed by cation-exchange chromatography. The protein migrated as a double band ...


    pub_type: 杂志文章


    authors: Mikes L,Man P

    更新日期:2003-07-01 00:00:00

  • Proteomic analysis of Entamoeba histolytica.

    abstract::In this study, the proteome of axenically grown Entamoeba histolytica parasites was explored by two-dimensional gel electrophoresis (2-DE), employing a practical and effective procedure for the solubilization of E. histolytica proteins. Approximately 900 protein species in the pH range between 4 and 7 were detected by...


    pub_type: 杂志文章


    authors: Tolstrup J,Krause E,Tannich E,Bruchhaus I

    更新日期:2007-02-01 00:00:00

  • Asparaginyl endopeptidase activity in adult Schistosoma mansoni.

    abstract::Sequence comparisons have recently shown that the Schistosoma mansoni protein Sm32 is similar to asparaginyl endoproteinases, a novel family of cysteine proteinases, of which the legumains from legumes are the best characterized. By synthesizing and employing fluorogenic peptide substrates for the specific detection o...


    pub_type: 杂志文章


    authors: Dalton JP,Hola-Jamriska L,Brindley PJ

    更新日期:1995-12-01 00:00:00

  • The formation of surface membrane vesicles from schistosomula of Schistosoma mansoni.

    abstract::Intact surface membrane vesicles were obtained and purified from the schistosomula of Schistosoma mansoni. The method of preparation of the vesicles involved treating the schistosomula in a buffered high salt medium for 1 h at 4 degrees C (Step 1), and then for 1 h at 25-37 degrees C (Step 2). Vesicles were formed fro...


    pub_type: 杂志文章


    authors: Kusel JR,Gazzinelli G,Colley DG,de Souza CP,Cordeiro MN

    更新日期:1984-12-01 00:00:00

  • Proteophosphoglycan is differentially expressed in sodium stibogluconate-sensitive and resistant Indian clinical isolates of Leishmania donovani.

    abstract::Leishmania produce several types of mucin-like glycoproteins called proteophosphoglycans (PPGs) some of which are secreted while others are found on the surface of promastigotes and amastigotes. These proteins are thought to be important in the transmission, invasion and subsequent intracellular survival of parasites....


    pub_type: 杂志文章


    authors: Samant M,Sahasrabuddhe AA,Singh N,Gupta SK,Sundar S,Dube A

    更新日期:2007-08-01 00:00:00

  • Infection success of different trematode genotypes in two alternative intermediate hosts: evidence for intraspecific specialization?

    abstract::The evolution of host specificity and the potential trade-off between being a generalist and a specialist are central issues in the evolutionary ecology of parasites. Different species of parasites or even different populations of the same species often show different degrees of host specificity. However, less is know...


    pub_type: 杂志文章


    authors: Leung TL,Poulin R

    更新日期:2010-02-01 00:00:00

  • Diverse single-amino-acid repeat profiles in the genus Cryptosporidium.

    abstract::Genome sequencing has greatly contributed to our understanding of parasitic protozoa. This is particularly the case for Cryptosporidium species (phylum Apicomplexa) which are difficult to propagate. Because of their polymorphic nature, simple sequence repeats have been used extensively as genotypic markers to differen...


    pub_type: 杂志文章


    authors: Widmer G

    更新日期:2018-08-01 00:00:00

  • Triggering and modulation of the host-parasite interplay by Echinococcus multilocularis: a review.

    abstract::As more facts emerge regarding the ways in which E. multilocularis-derived molecules trigger the host immune response and modulate the host-parasite interplay, it becomes possible to envisage how the parasite can survive and proliferate in its intermediate host, while in other hosts it dies out. Through effects on cel...


    pub_type: 杂志文章,评审


    authors: Mejri N,Hemphill A,Gottstein B

    更新日期:2010-03-01 00:00:00

  • The amount of acetylcholinesterase on the parasite surface reflects the differential sensitivity of schistosome species to metrifonate.

    abstract::Acetylcholinesterase (AChE) is present in all stages of the life-cycle of schistosomes and is located in muscle and on the surface of the parasite. Metrifonate is a drug that inhibits AChE. We compared the AChEs from three schistosome species (Schistosoma mansoni, Schistosoma haematobium and Schistosoma bovis) that ha...


    pub_type: 杂志文章


    authors: Camacho M,Tarrab-Hazdai R,Espinoza B,Arnon R,Agnew A

    更新日期:1994-02-01 00:00:00

  • Experimental test of host specificity in a behaviour-modifying trematode.

    abstract::Host behavioural modification by parasites is a common and well-documented phenomenon. However, knowledge on the complexity and specificity of the underlying mechanisms is limited, and host specificity among manipulating parasites has rarely been experimentally verified. We tested the hypothesis that the ability to in...


    pub_type: 杂志文章


    authors: Hernandez RN,Fredensborg BL

    更新日期:2015-11-01 00:00:00

  • Immunolocalization and characterization of the low molecular weight antigen (4-5 kDa) of Toxoplasma gondii that elicits an early IgM response upon primary infection.

    abstract::A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonst...


    pub_type: 杂志文章


    authors: Tomavo S,Couvreur G,Leriche MA,Sadak A,Achbarou A,Fortier B,Dubremetz JF

    更新日期:1994-02-01 00:00:00

  • Density-dependent processes in the transmission of human onchocerciasis: intensity of microfilariae in the skin and their uptake by the simuliid host.

    abstract::The transmission success of Onchocerca volvulus is thought to be influenced by a variety of regulatory or density-dependent processes that act at various points in the two-host life-cycle. This paper examines one component of the life-cycle, namely, the ingestion of microfilariae by the simuliid vector, to assess the ...


    pub_type: 杂志文章


    authors: Basáñez MG,Boussinesq M,Prod'hon J,Frontado H,Villamizar NJ,Medley GF,Anderson RM

    更新日期:1994-01-01 00:00:00

  • Host associations and turnover of haemosporidian parasites in manakins (Aves: Pipridae).

    abstract::Parasites of the genera Plasmodium and Haemoproteus (Apicomplexa: Haemosporida) are a diverse group of pathogens that infect birds nearly worldwide. Despite their ubiquity, the ecological and evolutionary factors that shape the diversity and distribution of these protozoan parasites among avian communities and geograp...


    pub_type: 杂志文章


    authors: Fecchio A,Svensson-Coelho M,Bell J,Ellis VA,Medeiros MC,Trisos CH,Blake JG,Loiselle BA,Tobias JA,Fanti R,Coffey ED,DE Faria IP,Pinho JB,Felix G,Braga EM,Anciães M,Tkach V,Bates J,Witt C,Weckstein JD,Ricklefs RE,

    更新日期:2017-06-01 00:00:00

  • An investigation of fructose utilization in Acanthocheilonema viteae.

    abstract::The capacity of Acanthocheilonema viteae to metabolize fructose was investigated in vitro. In common with other filarial species A. viteae oxidized fructose to lactate but its rate of consumption was only 40% of the glucose-containing control value. Fructose was not incorporated into glycogen. Release of 14CO2 from [U...


    pub_type: 杂志文章


    authors: Hayes DJ,Carter NS

    更新日期:1990-12-01 00:00:00

  • Molecular characterization of Thelastomatoidea (Nematoda: Oxyurida) from cockroaches in Australia.

    abstract::A molecular approach was used to genetically characterize 5 species (Aoruroides queenslandensis, Blattophila sphaerolaima, Cordonicola gibsoni, Desmicola ornata and Leidynemella fusiformis) belonging to the superfamily Thelastomatoidea (Nematoda: Oxyurida), a group of pinworms that parasitizes terrestrial arthropods. ...


    pub_type: 杂志文章


    authors: Jex AR,Hu M,Rose HA,Schneider M,Cribb TH,Gasser RB

    更新日期:2006-07-01 00:00:00

  • Anthelmintic activity of plants against gastrointestinal nematodes of goats: a review.

    abstract::The gastrointestinal nematodes (GIN) stand out as an important cause of disease in small ruminant, especially on goat farm. Widespread resistance to synthetic anthelminthics has stimulated the research for alternative strategies of parasite control, including the use of medicinal plants. The present work summarizes th...


    pub_type: 杂志文章


    authors: Oliveira Santos F,Ponce Morais Cerqueira A,Branco A,José Moreira Batatinha M,Borges Botura M

    更新日期:2019-09-01 00:00:00

  • Environmental sodium regulates cutaneous sugar transport in a digenean fluke.

    abstract::Glucose uptake was examined in adult specimens of Proterometra macrostoma (Trematoda: Digenea) recovered from the stomach (endoparasites) and gills (ectoparasites) of longear sunfish, Lepomis megalotis. The endoparasitic forms transported glucose directly through the external body surface by Na+-independent, facilitat...


    pub_type: 杂志文章


    authors: Uglem GL

    更新日期:1987-02-01 00:00:00

  • Synergistic antimalarial activity of ketones with rufigallol and vitamin C.

    abstract::Malaria remains a major cause of human morbidity and mortality worldwide. Plasmodium falciparum, the most virulent of the 4 human Plasmodium species causing malaria, is potentially life threatening, is increasing in prevalence and is becoming even more resistant to in-use drugs. In light of the growing problem of mult...


    pub_type: 杂志文章


    authors: Mahajan SS,Kamath VR,Ghatpande SS

    更新日期:2005-10-01 00:00:00

  • Isolation and characterization of an avian isolate of Encephalitozoon hellem.

    abstract::Members of the phylum Microspora are a group of unusual, obligate intracellular eukaryotic parasites that infect a wide range of hosts. However, there are a limited number of microsporidial infections reported in avian hosts, and no parasite species has been defined as an avian pathogen. A microsporidian organism was ...


    pub_type: 杂志文章


    authors: Snowden KF,Logan K,Phalen DN

    更新日期:2000-07-01 00:00:00

  • Amino acid catabolism in the nematodes Heligmosomoides polygyrus and Panagrellus redivivus. 1. Removal of the amino group.

    abstract::The major transaminase in Heligmosomoides polygyrus, Panagrellus redivivus and rat liver was the 2-oxoglutarate-glutamate system, with relatively few amino acids acting as donors for the pyruvate-alanine and oxaloacetate-aspartate systems. The relative effectiveness of the different amino acid donors in the three tran...


    pub_type: 杂志文章


    authors: Grantham BD,Barrett J

    更新日期:1986-12-01 00:00:00