Abstract:
:A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 10(4)-10(5) promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 10(6) cells of L. major, L. tropica, L. killicki, L. aethiopica, L. braziliensis and L. mexicana. A low degree of hybridization was found with L. donovani and L. chagasi. This probe will be of particular use in Mediterranean countries, such as Italy, in which dermotropic zymodemes of L. infantum can be difficult to isolate and grow in numbers sufficient for isoenzyme typing.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Gramiccia M,Smith DF,Angelici MC,Ready PD,Gradoni Ldoi
10.1017/s0031182000073650subject
Has Abstractpub_date
1992-08-01 00:00:00pages
29-34eissn
0031-1820issn
1469-8161journal_volume
105 ( Pt 1)pub_type
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