A kinetoplast DNA probe diagnostic for Leishmania infantum.

Abstract:

:A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 10(4)-10(5) promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 10(6) cells of L. major, L. tropica, L. killicki, L. aethiopica, L. braziliensis and L. mexicana. A low degree of hybridization was found with L. donovani and L. chagasi. This probe will be of particular use in Mediterranean countries, such as Italy, in which dermotropic zymodemes of L. infantum can be difficult to isolate and grow in numbers sufficient for isoenzyme typing.

journal_name

Parasitology

journal_title

Parasitology

authors

Gramiccia M,Smith DF,Angelici MC,Ready PD,Gradoni L

doi

10.1017/s0031182000073650

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

29-34

eissn

0031-1820

issn

1469-8161

journal_volume

105 ( Pt 1)

pub_type

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