Abstract:
:The amitochondriate parasitic protist Trichomonas vaginalis can utilize either glucose or maltose as carbon and energy source. The mechanisms of maltose utilization were explored with uptake experiments using radio-isotope labelled maltose in combination with the silicone-oil centrifugation technique and enzymatic assays measuring maltose hydrolysis. The uptake of maltose label became saturated after 2-3 h. The uptake of maltose as a function of the external maltose concentration was linear at low concentrations with no further increase at higher levels, kinetics characteristic of reactions obeying Michaelis-Menten kinetics preceded by a diffusion-limited step. Increased viscosity of the medium resulted in decreased maltose uptake, indicating an extracellular location of the diffusion-limited step. Most of the cellular alpha-glucosidase activity of T. vaginalis was detected on the cell surface, suggesting that maltose is hydrolysed to glucose outside the cell. Glucose interfered more with maltose uptake, and maltose less with glucose uptake, than would be expected if 1 mol of maltose were the equivalent of 2 mol of glucose. This pattern of interaction indicated that the interference occurs before the common metabolic pathway and even before the transport step, supporting the idea of extracellular maltose hydrolysis. We conclude that maltose is hydrolysed to glucose in the boundary layer of the cell, a process akin to membrane digestion in vertebrate enterocytes and on the teguments of helminths. The glucose formed is then transported by the glucose carrier of the organism.
journal_name
Parasitologyjournal_title
Parasitologyauthors
ter Kuile BH,Müller Mdoi
10.1017/s0031182000081026subject
Has Abstractpub_date
1995-01-01 00:00:00pages
37-44eissn
0031-1820issn
1469-8161journal_volume
110 ( Pt 1)pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::Guinea worm disease, dracunculiasis or dracontiasis, is an ancient disease with records going back over 4500 years, but until the beginning of the 20th century, little was known about its life cycle, particularly how humans became infected. In 1905, Robert Thomas Leiper was sent by the British colonial authorities to ...
journal_title:Parasitology
pub_type: 传,历史文章,杂志文章,评审
doi:10.1017/S0031182017000683
更新日期:2017-10-01 00:00:00
abstract::Neurocysticercosis (NCC) is the most common parasitic disease of the central nervous system (CNS) caused by the larval form of the tapeworm Taenia solium. NCC has a long asymptomatic period with little or no inflammation, and the sequential progression to symptomatic NCC depends upon the intense inflammation associate...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182009991971
更新日期:2010-03-01 00:00:00
abstract::Parasites have a major impact on global finfish and shellfish aquaculture, having significant effects on farm production, sustainability and economic viability. Parasite infections and impacts can, according to pathogen and context, be considered to be either unpredictable/sporadic or predictable/regular. Although bot...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182014001437
更新日期:2015-01-01 00:00:00
abstract::The presence of introduced hosts can increase or decrease infections of co-introduced parasites in native species of conservation concern. In this study, we compared parasite abundance, intensity, and prevalence between native Awaous stamineus and introduced poeciliid fishes by a co-introduced nematode parasite (Camal...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001001
更新日期:2016-10-01 00:00:00
abstract::Eggs of Neoheterocotyle rhinobatidis, Troglocephalus rhinobatidis and Merizocotyle icopae (Monogenea: Monocotylidae) from the shovelnose ray Rhinobatos typus (Rhinobatidae) have a distinct hatching pattern linked to light periodicity. Larvae of these 3 monogenean species emerge only during daylight when exposed to nat...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118209900637x
更新日期:2000-09-01 00:00:00
abstract::The accumulation of the trypanocide isometamidium chloride (Samorin, RMB Animal Health Ltd., UK) by a range of clones of Trypanosoma congolense with varying sensitivity to the drug, was measured by methods based on the fluorescence of isometamidium. Fluorescence microscopy and flow cytometry showed a reduction in drug...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000059527
更新日期:1991-10-01 00:00:00
abstract::Nematodes represent one of the most abundant and species-rich groups of animals on the planet, with parasitic species causing chronic, debilitating infections in both livestock and humans worldwide. The prevalence and success of the nematodes is a direct consequence of the exceptionally protective properties of their ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182010001113
更新日期:2011-02-01 00:00:00
abstract::In recent years, through a national programme for schistosomiasis control, this infection has been eliminated from Iran. The aim of this study was to report the process of significant decrease of urogenital schistosomiasis in southwestern Iran. During national programme surveillance for urogenital schistosomiasis cont...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000883
更新日期:2016-09-01 00:00:00
abstract::Chagas infection is a major endemic disease affecting Latin American countries. The persistence of Trypanosoma cruzi generates a chronic inflammatory reactivity that induces an immune response directed to the host's tissues. The effectiveness of the treatment in the chronic phase is still unsatisfactory due, amongst o...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013000103
更新日期:2013-06-01 00:00:00
abstract::Treatment of BALB/c or MF1 mice with cyclosporin A (CsA) around the time of infection with Schistosoma mansoni conferred almost complete protection. The migration kinetics of L-[75Se]selenomethionine-labelled infective cercariae were investigated by compressed tissue autoradiography. Similar levels of skin penetration...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060340
更新日期:1991-02-01 00:00:00
abstract::The incidence of fever among infants in the village of Idete in the Morogoro region of Tanzania was analyzed in relation to densities of Plasmodium falciparum parasites in the peripheral blood. Parasite densities in both fever cases and in asymptomatic infants, were compared and a Bayesian non-parametric mixture decom...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099005211
更新日期:2000-01-01 00:00:00
abstract::While purine transport has been widely studied in protozoa, almost nothing is known about their capacity to salvage pyrimidines. Here, we report a Leishmania major transporter with high affinity for uracil (Km=0.32+/-0.07 microM) which we designated LmU1. This transporter displayed a high degree of specificity, as it ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006626
更新日期:2005-03-01 00:00:00
abstract::Merocyanin 540 (Mc540) is a fluorescent compound which is thought to bind to membranes in which there are substantial amounts of lipid in the lipid-crystalline phase. It is shown here to be of value in detecting the transformation by both mechanical and skin-penetration methods of the cercaria to the schistosomulum. T...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000081671
更新日期:1988-02-01 00:00:00
abstract::The blackcap (Sylvia atricapilla) is a common Palearctic migratory warbler, and haemosporidian parasites are common in this species. However, genetic and phenotypic diversity of haemosporidians in warblers has been insufficiently investigated and poorly linked. We addressed this issue by combining molecular and micros...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009991235
更新日期:2010-02-01 00:00:00
abstract::The current trend of antimony (Sb) unresponsiveness in the Indian subcontinent is a major impediment to effective chemotherapy of visceral leishmaniasis (VL). Although contributory mechanisms studied in laboratory-raised Sb-R parasites include an up-regulation of drug efflux pumps and increased thiols, their role in c...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182007003150
更新日期:2007-11-01 00:00:00
abstract::Cystic echinococcosis (CE) is a high prevalence zoonosis among the rural population of Tacuarembó (Uruguay). The correlation between serological data and the incidence of risk factors was studied in a survey carried out in 1998 among rural communities where 480 individuals were examined by means of abdominal sonograph...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006717
更新日期:2005-04-01 00:00:00
abstract::This study assessed the role of historical processes on the geographic isolation, molecular evolution, and morphological diversification of host-parasite populations from the southern Brazilian coast. Adult specimens of Scleromystax barbatus and Scleromystax macropterus were collected from the sub-basin of the Nhundia...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019000556
更新日期:2019-09-01 00:00:00
abstract::We present a new phylogenetic analysis of 15 primate Plasmodium species based on 18S rDNA sequences including new sequences of Plasmodium coatneyi, P. fieldi, P. gonderi, P. hylobati and P. simium. The results are discussed in the context of the parasite host species and their geographical distribution. Contrary to ot...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006146
更新日期:2004-12-01 00:00:00
abstract::Aspects of vitellogenesis, known to be controlled by juvenile hormone, are adversely affected by Hymenolepis diminuta infection of Tenebrio molitor, in spite of circulating titres of the hormone remaining unchanged. It has therefore been proposed that juvenile hormone binding is disrupted at the tissue site level. Juv...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000065288
更新日期:1995-06-01 00:00:00
abstract::The magnetic resonance imaging (MRI) appearance of the brain and spinal cord in humans with neuroangiostrongyliasis (NA) due to Angiostrongylus cantonensis infection has been well reported. Equivalent studies in animals are lacking. This case series describes clinical and MRI findings in 11 dogs with presumptively or ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182020001742
更新日期:2020-09-21 00:00:00
abstract::We examined the mechanism of action and compared the anthelmintic efficacy of cysteine proteinases from papaya, pineapple, fig, kiwi fruit and Egyptian milkweed in vitro using the rodent gastrointestinal nematode Heligmosomoides polygyrus. Within a 2 h incubation period, all the cysteine proteinases, with the exceptio...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006225
更新日期:2005-02-01 00:00:00
abstract::Solid experimental evidence indicates that EBA-175 is used as a ligand by the Plasmodium falciparum merozoite to bind to human RBC, via different binding processing fragments. Using synthetic peptides and specific receptor-ligand interaction methodology, we have identified 6 high-activity binding sequences from the EB...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118209900551x
更新日期:2000-03-01 00:00:00
abstract::The pre-feeding and feeding periods of larvae, nymphs and adults of Rhipicephalus appendiculatus on rabbits (Oryctolagus cuniculus) were investigated. Larvae and nymphs required at least 8-9 days after hatching and moulting respectively before they could attach and start feeding, while adults required at least 6-9 day...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000043195
更新日期:1976-02-01 00:00:00
abstract::The abundance of Profilicollis botulus was monitored in eider ducks for 3 years. No significant difference in P. botulus abundance was found between eiders taken alive and those collected dead from the shore line, or between adult males and adult females. Adult male eiders had a seasonal pattern of infection. Incubati...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000062806
更新日期:1985-12-01 00:00:00
abstract::Trichinella spiralis infections may lead to the loss of behavioural dominance among male mice. Reversals of dominance order first appear at the time when newborn larvae are released into the circulation of the host. The duration of dominance reversals bears no relationship to the number of muscle larvae harboured. ...
journal_title:Parasitology
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::This study describes morphological changes during capsule formation of Trichinella spiralis with emphasis on nuclei within the capsule. The nuclei of the pre-existing muscle cell may become hypertrophied or degrade soon after the entrance of newborn larvae. Cytoplasm associated with these hypertrophied nuclei was baso...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099006198
更新日期:2000-08-01 00:00:00
abstract::A survey was carried out to determine values for the prevalence and intensity of infection of intestinal helminths in children living in Cocle Province, Republic of Panama. The data set consisted of the results of microscopic examinations (modified Kato Katz technique) of stool samples collected in November 1987 from ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000058741
更新日期:1989-10-01 00:00:00
abstract::The multicopy mini-exon-derived RNA (med RNA) locus of Leishmania donovani was enzymatically amplified by the polymerase chain reaction (PCR). The major 180 bp PCR product contained conserved med RNA gene sequences flanking the variable intergenic spacer from the med RNA gene tandem repeat. The oligonucleotide primers...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000068086
更新日期:1993-12-01 00:00:00
abstract::The effects of a parasitic infection with the nematode Nippostrongylus brasiliensis on the degradation rates of cytoplasmic tRNA, rRNA and mRNA in rats have been investigated by measuring the renal excretion rates of the modified RNA catabolites N6-threoninocarbonyladenosine, pseudouridine and 7-methylguanine. Between...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182097002102
更新日期:1998-02-01 00:00:00
abstract::A cDNA clone codifying ribosomal protein L25 was isolated from a Leishmania braziliensis cDNA gene library. The alignment of the amino acid sequence deduced from this gene with other proteins revealed that this protein is related to the L23/25 rihosomal protein family. This is so because this protein shows, in its C-t...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182002001804
更新日期:2002-07-01 00:00:00