Temperature gradient gel electrophoresis (TGGE) analysis of riboprints from Trypanosoma cruzi.

Abstract:

:To test the homogeneity of 18S sequences within Trypanosoma cruzi, riboprint profiles were separated by temperature gradient gel electrophoresis (TGGE). Upon interpretation of melting curves of fragments within a riboprint profile, there appeared to be two 18S sequence types within each stock examined. Two similar types were also observed within outgroup taxa Trypanosoma conorhini, Trypanosoma rangeli and Leishmania braziliensis. From DNA hybridization studies, these fragments were shown to have homology to the 18S V1 region. There are therefore two 18S V1 regions, differing in sequence, present in all taxa examined. When only a single 18S sequence is used to represent each taxa for phylogenetic inference, comparisons may be between paralogous and not orthologous copies of this region, such that, inferred relationships may merely reflect a gene history. This seriously questions the current molecular phylogeny of these protozoa using 18S data.

journal_name

Parasitology

journal_title

Parasitology

authors

Stothard JR,Frame IA,Carrasco HJ,Miles MA

doi

10.1017/s0031182098002972

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

249-53

eissn

0031-1820

issn

1469-8161

journal_volume

117 ( Pt 3)

pub_type

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