Abstract:
:Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet genotypically distinct. Another probe, R-2, while not distinguishing between the two forms of E. hystolytica, was able to differentiate between them and E. moshkovskii, which has morphologically identical cysts and trophozoites. A third probe, BR-1, identified strain-specific RFLPs.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Cruz-Reyes JA,Spice WM,Rehman T,Gisborne E,Ackers JPdoi
10.1017/s0031182000061679subject
Has Abstractpub_date
1992-04-01 00:00:00pages
239-46eissn
0031-1820issn
1469-8161journal_volume
104 ( Pt 2)pub_type
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