Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica.

Abstract:

:Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet genotypically distinct. Another probe, R-2, while not distinguishing between the two forms of E. hystolytica, was able to differentiate between them and E. moshkovskii, which has morphologically identical cysts and trophozoites. A third probe, BR-1, identified strain-specific RFLPs.

journal_name

Parasitology

journal_title

Parasitology

authors

Cruz-Reyes JA,Spice WM,Rehman T,Gisborne E,Ackers JP

doi

10.1017/s0031182000061679

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

239-46

eissn

0031-1820

issn

1469-8161

journal_volume

104 ( Pt 2)

pub_type

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