The formation of surface membrane vesicles from schistosomula of Schistosoma mansoni.

Abstract:

:Intact surface membrane vesicles were obtained and purified from the schistosomula of Schistosoma mansoni. The method of preparation of the vesicles involved treating the schistosomula in a buffered high salt medium for 1 h at 4 degrees C (Step 1), and then for 1 h at 25-37 degrees C (Step 2). Vesicles were formed from mechanically- and skin-transformed schistosomula, and their size and number depended on the temperature of Step 2. The majority of the vesicles expressed surface membrane concanavalin A receptors and parasite antigens on their outermost surfaces. When incubated with sensitized peripheral blood mononuclear cell preparations from humans exposed to schistosomal preparations, the vesicles stimulated lymphocyte transformation as effectively as soluble egg and adult worm antigens. Although both preparations contained identical proteins, small vesicles were less effective than large vesicles in stimulating lymphocyte transformation. Hence, vesicles prepared under a variety of conditions might be used to study those factors which influence the presentation of membrane-bound surface antigens to the immune system of the host.

journal_name

Parasitology

journal_title

Parasitology

authors

Kusel JR,Gazzinelli G,Colley DG,de Souza CP,Cordeiro MN

doi

10.1017/s0031182000056717

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

483-94

eissn

0031-1820

issn

1469-8161

journal_volume

89 ( Pt 3)

pub_type

杂志文章
  • Purification of an agglutinin from the haemolymph of the snail Bulinus nasutus and demonstration of related proteins in other Bulinus spp.

    abstract::The snail Bulinus nasutus 1214 possesses a potent haemagglutinin (end-point titre with human erythrocytes, 2(-18)) in its cell-free haemolymph which also binds to the miracidia (but not other larvae) of the incompatible parasite Schistosoma margrebowiei. We have purified a protein possessing this haemagglutinating pro...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000074928

    authors: Harris RA,Preston TM,Southgate VR

    更新日期:1993-02-01 00:00:00

  • Eimeria species which infect the chicken contain virus-like RNA molecules.

    abstract::There is increasing support for the presence of viruses and virus-like particles inside protozoan cells. This study describes viral-like RNA molecules that have been detected in two species of Eimeria that infect the chicken. The RNA molecule identified in E. maxima has been characterized: subcellular fractionation st...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063198

    authors: Ellis J,Revets H

    更新日期:1990-10-01 00:00:00

  • The parasitism of Schistosoma mansoni (Digenea-Trematoda) in a naturally infected population of water rats, Nectomys squamipes (Rodentia-Sigmodontinae) in Brazil.

    abstract::Schistosomiasis is a health problem in Brazil and the role of rodents in maintaining the schistosome life-cycle requires further clarification. The influence of Schistosoma mansoni on a population of Nectomys squamipes was studied by capture-recapture (1st phase, from June 1991 to November 1995) and removal (2nd phase...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005892

    authors: D'Andrea PS,Maroja LS,Gentile R,Cerqueira R,Maldonado Júnior A,Rey L

    更新日期:2000-06-01 00:00:00

  • Modelling the influence of host community composition in a sylvatic Trypanosoma cruzi system.

    abstract::Species composition of wild reservoir hosts can influence the transmission and maintenance of multi-host vector borne pathogens. The 'pace of life' hypothesis proposes that the life history strategy of reservoir hosts can influence pathogen transmission of vector borne generalist pathogens. We use empirical data to pa...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017001287

    authors: Erazo D,Cordovez J,Cabrera C,Calzada JE,Saldaña A,Gottdenker NL

    更新日期:2017-12-01 00:00:00

  • Dynamic interaction between CD4+ T cells and parasitic helminths: mathematical models of heterogeneity in outcome.

    abstract::Potential mechanisms of immunoregulation have been investigated for the capacity to generate heterogeneity in the outcome of infection with helminth parasites. We have developed a mathematical model of the interaction between T cell and parasite populations, based on the assumption that activation of a Th1 CD4+ T cell...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000074692

    authors: Schweitzer AN,Anderson RM

    更新日期:1992-12-01 00:00:00

  • Proteomic analysis of a 120 kDa protein complex in cyst fluid of Taenia solium metacestode and preliminary evaluation of its value for the serodiagnosis of neurocysticercosis.

    abstract::Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of p...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005008504

    authors: Lee EG,Bae YA,Jeong YT,Chung JY,Je EY,Kim SH,Na BK,Ju JW,Kim TS,Ma L,Cho SY,Kong Y

    更新日期:2005-12-01 00:00:00

  • Classical transmitters and their receptors in flatworms.

    abstract::The flatworm nervous system employs a wide repertoire of neuroactive substances, including small chemical messengers, the so called classical transmitters, and several types of neuropeptides. A large body of research accumulated over four decades has provided a wealth of information on the tissue localization and effe...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182005008565

    authors: Ribeiro P,El-Shehabi F,Patocka N

    更新日期:2005-01-01 00:00:00

  • Glomerular disease associated with Polysporoplasma sparis (Myxozoa) infections in cultured gilthead sea bream, Sparus aurata L. (Pisces: Teleostei).

    abstract::Polysporoplasma sparis infection was studied in gilthead sea bream from different mariculture systems of the Spanish coasts. Culture conditions influenced the infection dynamics, as the parasite appeared only in semi-intensive cultures and was not found in intensive closed systems nor in open ones. No clear seasonal p...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182098003758

    authors: Palenzuela O,Alvarez-Pellitero P,Sitjà-Bobadilla A

    更新日期:1999-03-01 00:00:00

  • Immune modulation by a high molecular weight fraction from the rat tapeworm Hymenolepis diminuta.

    abstract::The host-parasite relationship is exquisitely specific. In exploiting the host niche, a variety of helminth parasites have been shown to directly manipulate their hosts' immune responses. We assessed the ability of a whole-worm extract of Hymenolepis diminuta to modulate immune cell activation. Immune cells isolated f...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004006985

    authors: Wang A,McKay DM

    更新日期:2005-05-01 00:00:00

  • Membrane localization and demonstration of isoforms of nucleoside triphosphate hydrolase from Toxoplasma gondii.

    abstract::Toxoplasma gondii has a unique enzyme, a NTPase, which has a wide specificity toward NTP. In the present study, we produced a monoclonal antibody (IgG1, 6C6) against the enzyme which could recognize NTPase isozymes among several strains of T. gondii. Three avirulent strains of T. gondii, ME49, Beverley and Nakayama, w...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000007101

    authors: Kikuchi T,Furuta T,Kojima S

    更新日期:2001-01-01 00:00:00

  • Simple epidemiological model predicts the relationships between prevalence and abundance in ixodid ticks.

    abstract::We tested whether the prevalence of ticks can be predicted reliably from a simple epidemiological model that takes into account only mean abundance and its variance. We used data on the abundance and distribution of larvae and nymphs of 2 ixodid ticks parasitic on small mammals (Apodemus agrarius, Apodemus flavicollis...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006001296

    authors: Stanko M,Krasnov BR,Miklisova D,Morand S

    更新日期:2007-01-01 00:00:00

  • Physico-chemical determinants of helminth component community structure in whitefish (Coregonus clupeaformes) from adjacent lakes in Northern Alberta, Canada.

    abstract::Populations of hosts vary extensively in the types and numbers of parasites that the average individual contains. Understanding the factors that lead to this variation is an important goal for parasite ecologists. We characterized patterns of helminth component community structure in whitefish collected from a cluster...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005008371

    authors: Goater CP,Baldwin RE,Scrimgeour GJ

    更新日期:2005-11-01 00:00:00

  • The alteration in signal transduction parameters induced by the excretory-secretory product from Giardia lamblia.

    abstract::The mechanism by which Giardia lamblia exerts its pathogenicity is likely to be multifactorial. A 58 kDa enterotoxin was purified and characterized from the excretory-secretory product (ESP) of the parasite (Kaur et al. 2001). In the present study an attempt has been made to elucidate the mechanism of action of the ES...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004005852

    authors: Shant J,Ghosh S,Bhattacharyya S,Ganguly NK,Majumdar S

    更新日期:2004-10-01 00:00:00

  • The ultrastructure and immunogold labelling of pancreatic polypeptide-immunoreactive cells associated with the egg-forming apparatus of a monogenean parasite, Diclidophora merlangi.

    abstract::An electron microscopical examination has been made of the fine structure and disposition of pancreatic polypeptide immunoreactive cells associated with the egg-forming apparatus in Diclidophora merlangi. The cell bodies are positioned in the parenchyma surrounding the ootype and taper to axon-like processes that exte...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000064404

    authors: Halton DW,Brennan GP,Maule AG,Shaw C,Johnston CF,Fairweather I

    更新日期:1991-06-01 00:00:00

  • Epidemiological investigation of a severe rumen fluke outbreak on an Irish dairy farm.

    abstract::Although the rumen fluke, Calicophoron daubneyi is now very common and widespread throughout Western Europe, reports of clinical cases are still rare. This study explores the epidemiological background to a severe rumen fluke outbreak in 6-month-old heifers on a dairy farm in Ireland. Sequence analysis of the cytochro...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017002086

    authors: O'Shaughnessy J,Garcia-Campos A,McAloon CG,Fagan S,de Waal T,McElroy M,Casey M,Good B,Mulcahy G,Fagan J,Murphy D,Zintl A

    更新日期:2018-06-01 00:00:00

  • Improved serodiagnosis of alveolar echinococcosis of humans using an in vitro-produced Echinococcus multilocularis antigen.

    abstract::Serology is an important tool for the diagnosis of alveolar echinococcosis (AE) in humans. In order to improve serodiagnostic performance, we have developed an in vitro-produced Echinococcus mulilocularis metacestode vesicle fluid (EmVF) antigen for application in an immunoblot assay. Immunoblot analysis of EmVF revea...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006002083

    authors: Müller N,Frei E,Nuñez S,Gottstein B

    更新日期:2007-06-01 00:00:00

  • Electron microscopy of Eimeria acervulina macrogametogony and oocyst wall formation.

    abstract::Macrogametogony and the formation of the oocyst wall has been examined in Eimeria acervulina. Macrogametes develop within a parasitophorous vacuole. Within the cytoplasm can be observed wall-forming bodies of Type I (WFBI) and Type II (WFBII), a nucleus, mitochondria, canaliculi and polysaccharide granules. WFBII are ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000001074

    authors: Pittilo RM,Ball SJ

    更新日期:1984-08-01 00:00:00

  • What helminth genomes have taught us about parasite evolution.

    abstract::The genomes of more than 20 helminths have now been sequenced. Here we perform a meta-analysis of all sequenced genomes of nematodes and Platyhelminthes, and attempt to address the question of what are the defining characteristics of helminth genomes. We find that parasitic worms lack systems for surface antigenic var...

    journal_title:Parasitology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1017/S0031182014001449

    authors: Zarowiecki M,Berriman M

    更新日期:2015-02-01 00:00:00

  • Sub-cellular fractionation of Trypanosoma brucei. Isolation and characterization of plasma membranes.

    abstract::A procedure is described for the isolation of sub-cellular fractions from bloodstream forms of Trypanosoma brucei. The method leaves intact most of the nuclei, mitochondria and microbodies. All the fractions have been chemically characterized and tested for 10 enzymatic markers. About 5% of total cell protein was isol...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000000974

    authors: Rovis L,Baekkeskov S

    更新日期:1980-06-01 00:00:00

  • Integrating the landscape epidemiology and genetics of RNA viruses: rabies in domestic dogs as a model.

    abstract::Landscape epidemiology and landscape genetics combine advances in molecular techniques, spatial analyses and epidemiological models to generate a more real-world understanding of infectious disease dynamics and provide powerful new tools for the study of RNA viruses. Using dog rabies as a model we have identified how ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S003118201200090X

    authors: Brunker K,Hampson K,Horton DL,Biek R

    更新日期:2012-12-01 00:00:00

  • Detection of Neospora caninum in aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin, China.

    abstract::Neospora caninum infection is a significant cause of abortion in cattle. We investigated the tissue distribution of N. caninum in aborted bovine fetuses and dam blood samples by a nested PCR assay, and compared the nested PCR with ELISA in the diagnosis of N. caninum infection. In total, 26 aborted fetuses and 813 blo...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009990813

    authors: Yao L,Yang N,Liu Q,Wang M,Zhang W,Qian WF,Hu YF,Ding J

    更新日期:2009-09-01 00:00:00

  • Diversity, distribution and biogeographical origins of Plasmodium parasites from the New Zealand bellbird (Anthornis melanura).

    abstract::Understanding the origin of invasive parasites and ecological transmission barriers on the distribution of mosquito-borne pathogens is enriched by molecular phylogenetic approaches now that large databases are becoming available. Here we assess the biogeographical relationships among haemosporidian blood parasites and...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011001491

    authors: Baillie SM,Brunton DH

    更新日期:2011-12-01 00:00:00

  • Economic costs of protistan and metazoan parasites to global mariculture.

    abstract::Parasites have a major impact on global finfish and shellfish aquaculture, having significant effects on farm production, sustainability and economic viability. Parasite infections and impacts can, according to pathogen and context, be considered to be either unpredictable/sporadic or predictable/regular. Although bot...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182014001437

    authors: Shinn AP,Pratoomyot J,Bron JE,Paladini G,Brooker EE,Brooker AJ

    更新日期:2015-01-01 00:00:00

  • In vitro cultivation of Trypanosoma congolense bloodstream forms in the absence of feeder cell layers.

    abstract::Bloodstream forms of Trypanosoma congolense (2 clones: ILNat3.1 and IL3000, and 4 stocks: IL2079, IL2466, IL3266 and CP-81) were continuously cultivated in vitro at 34-36 degrees C in the absence of feeder cell layers, using HMI-93 medium which was modified from Iscove's modified Dulbecco's MEM (Flow Laboratories, Irv...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000062533

    authors: Hirumi H,Hirumi K

    更新日期:1991-04-01 00:00:00

  • Changes in the zymogenic cell populations of the abomasa of sheep infected with Haemonchus contortus.

    abstract::The effects of dietary urea supplementation and of a 10-week trickle infection regime, simulating chronic exposure to Haemonchus contortus, on the zymogenic population of the abomasa of Hampshire Down lambs was examined. At necropsy a variety of parameters including plasma pepsinogen concentration, the wet weights of ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182098002704

    authors: Scott I,Stear MJ,Irvine J,Dick A,Wallace DS,McKellar QA

    更新日期:1998-06-01 00:00:00

  • Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism.

    abstract::Isolates of Trypanosoma cruzi from human patients, domestic and sylvatic animals and vector insects were obtained in different areas of Argentina. Electrophoretic patterns of enzymes from extracts of 95 isolates were analysed. On the basis of zymograms providing information on 10 loci, 12 zymodemes are described accor...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000067755

    authors: de Luca D'oro GM,Gardenal CN,Perret B,Crisci JV,Montamat EE

    更新日期:1993-11-01 00:00:00

  • Molecular mechanisms involved in the transport of antibiotics into bacteria.

    abstract::Many clinically useful antibacterial drugs have intracellular target sites. Therefore, in order to reach their targets, these compounds must be able to cross bacterial outer and cytoplasmic membranes. Considerable information is available on the mechanisms by which antibiotics cross bacterial membranes and, in many ca...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182000085966

    authors: Chopra I

    更新日期:1988-01-01 00:00:00

  • Magnetic resonance imaging in dogs with neuroangiostrongyliasis (rat lungworm disease).

    abstract::The magnetic resonance imaging (MRI) appearance of the brain and spinal cord in humans with neuroangiostrongyliasis (NA) due to Angiostrongylus cantonensis infection has been well reported. Equivalent studies in animals are lacking. This case series describes clinical and MRI findings in 11 dogs with presumptively or ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020001742

    authors: Wun MK,Malik R,Yu J,Chow KE,Lau M,Podadera JM,Webster N,Lee R,Šlapeta J,Davies S

    更新日期:2020-09-21 00:00:00

  • Parasites as biological tags of fish stocks: a meta-analysis of their discriminatory power.

    abstract::The use of parasites as biological tags to discriminate among marine fish stocks has become a widely accepted method in fisheries management. Here, we first link this approach to its unstated ecological foundation, the decay in the similarity of the species composition of assemblages as a function of increasing distan...

    journal_title:Parasitology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1017/S0031182013001534

    authors: Poulin R,Kamiya T

    更新日期:2015-01-01 00:00:00

  • Immunochemical analysis of surface membrane antigens on erythrocytes infected with non-cloned SICA[+] or cloned SICA[-] Plasmodium knowlesi.

    abstract::The SICA[-] or non-agglutinable phenotype of Plasmodium knowlesi schizont-infected erythrocytes has been defined serologically but not biochemically. Similarly, non-cloned SICA[+] or agglutinable parasites have been shown serologically to express SICA or variant antigen(s) but the number and nature of such antigens ha...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000057346

    authors: Howard RJ,Barnwell JW

    更新日期:1985-10-01 00:00:00