Immune modulation by a high molecular weight fraction from the rat tapeworm Hymenolepis diminuta.

Abstract:

:The host-parasite relationship is exquisitely specific. In exploiting the host niche, a variety of helminth parasites have been shown to directly manipulate their hosts' immune responses. We assessed the ability of a whole-worm extract of Hymenolepis diminuta to modulate immune cell activation. Immune cells isolated from human blood or rodent spleens were activated with the T cell mitogen, concanavalin A (Con A) +/- H. diminuta extract and cytokine production (i.e. IL-2, -4, -10, -12) and proliferation assessed by ELISA and [3H]thymidine incorporation 24 and 72 h post-treatment, respectively. Co-treatment with the H. diminuta extract (100 microg protein/ml) virtually abolished Con A-induced immune cell proliferation, which was not due to increased apoptosis. Boiling of the worm extract reduced its anti-proliferative effect and fractionation indicated that a > 50 kDa component was predominantly responsible for the inhibition of Con A-induced immune cell proliferation. Cytokine determinations revealed that the H. diminuta extract significantly reduced Con A-stimulated IL-2 and IL-4, but enhanced the production of IFNy, IL-12 and IL-10. The increased IL-12 was due to an LPS contaminant in the extract and a helminth-derived 'IL-12'-like peptide that bound in the ELISA and Western blots. In contrast, a H. diminuta-derived factor directly stimulated IL-10 production by murine splenocytes, and contaminating LPS synergistically enhanced the production of IL-10. Thus, H. diminuta has the potential to block stimulated T cell proliferation and, by inhibiting IL-4 and promoting IL-10 production, may bias the immune environment towards one of immunoregulation and away from IL-4 dominated T helper 2 type events.

journal_name

Parasitology

journal_title

Parasitology

authors

Wang A,McKay DM

doi

10.1017/s0031182004006985

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

575-85

issue

Pt 5

eissn

0031-1820

issn

1469-8161

journal_volume

130

pub_type

杂志文章
  • The specificity of purine base and nucleoside uptake in promastigotes of Leishmania braziliensis panamensis.

    abstract::Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported a...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055256

    authors: Hansen BD,Perez-Arbelo J,Walkony JF,Hendricks LD

    更新日期:1982-10-01 00:00:00

  • Kinetic modelling of isometamidium chloride (Samorin) uptake by Trypanosoma congolense.

    abstract::Clones of Trypanosoma congolense which express resistance to the widely used trypanocide isometamidium chloride accumulate less of the drug than clones which are sensitive to drug treatment. A mathematical model has been developed which was able to predict theoretical lines representing the uptake kinetics in trypanos...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000073728

    authors: Sutherland IA,Mounsey A,Eisler M,Holmes PH

    更新日期:1992-08-01 00:00:00

  • Down-regulation of murine susceptibility to cerebral malaria by inoculation with third-stage larvae of the filarial nematode Brugia pahangi.

    abstract::In areas where malaria is endemic, helminthic infections, caused by intestinal or filarial parasites, commonly coexist with malaria in the same individual. This study investigates the course of Plasmodium berghei malaria infection in CBA/J mice inoculated with irradiated attenuated 3rd-stage larvae (L3) of Brugia paha...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182096008566

    authors: Yan Y,Inuo G,Akao N,Tsukidate S,Fujita K

    更新日期:1997-04-01 00:00:00

  • Phylogeny of snake trypanosomes inferred by SSU rDNA sequences, their possible transmission by phlebotomines, and taxonomic appraisal by molecular, cross-infection and morphological analysis.

    abstract::Blood examination by microhaematocrit and haemoculture of 459 snakes belonging to 37 species revealed 2.4% trypanosome prevalence in species of Viperidae (Crotalus durissus and Bothrops jararaca) and Colubridae (Pseudoboa nigra). Trypanosome cultures from C. durissus and P. nigra were behaviourally and morphologically...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008004253

    authors: Viola LB,Campaner M,Takata CS,Ferreira RC,Rodrigues AC,Freitas RA,Duarte MR,Grego KF,Barrett TV,Camargo EP,Teixeira MM

    更新日期:2008-04-01 00:00:00

  • Contrasting regulation of fecundity in two abomasal nematodes of Svalbard reindeer (Rangifer tarandus platyrhynchus).

    abstract::Stability of trichostrogylid populations indicates that some form of density-dependent regulation occurs which could act through fecundity. We present evidence for intraspecific density-dependent effects in 1 of 2, dominant, abomasal nematodes species (Ostertagia gruehneri) of Svalbard reindeer (Rangifer tarandus plat...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001007818

    authors: Irvine RJ,Stien A,Dallas JF,Halvorsen,Langvatn R,Albon SD

    更新日期:2001-06-01 00:00:00

  • Temperature-related parasite infection dynamics: the case of proliferative kidney disease of brown trout.

    abstract::Climate change, in particular rising temperature, is suspected to be a major driver for the emergence of many wildlife diseases. Proliferative kidney disease of salmonids, caused by the myxozoan Tetracapsuloides bryosalmonae, was used to evaluate how temperature dependence of host-parasite interactions modulates disea...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017001482

    authors: Strepparava N,Segner H,Ros A,Hartikainen H,Schmidt-Posthaus H,Wahli T

    更新日期:2018-03-01 00:00:00

  • Nitric oxide interaction with IL-10, MIP-1alpha, MCP-1 and RANTES over the in vitro granuloma formation against different Schistosoma mansoni antigenic preparations on human schistosomiasis.

    abstract::Nitric oxide (NO) produced by cytokine-activated macrophages is reported to be cytotoxic against the helminth Schistosoma mansoni, although this is a controversial issue. Previous work in our laboratory identified a fraction of S. mansoni soluble adult worm antigenic preparation (SWAP), named PIII, able to elicit sign...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005636

    authors: Oliveira DM,Silva-Teixeira DN,Gustavson S,Oliveira SM,Goes AM

    更新日期:2000-04-01 00:00:00

  • Chagas' disease: reactivity against homologous tissues induced by different strains of Trypanosoma cruzi.

    abstract::We have previously reported that the mechanisms of neuromyopathic damage induced by Trypanosoma cruzi are mediated by T cells and are parasite-strain dependent. In the present study our aim was to determine whether the humoral response against muscle and nervous system is also parasite-strain dependent. Of the sera fr...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182097001625

    authors: Tekiel VS,Mirkin GA,Gonzalez Cappa SM

    更新日期:1997-11-01 00:00:00

  • Plagiorchis elegans: emergence, longevity and infectivity of cercariae, and host behavioural modifications during cercarial emergence.

    abstract::We investigated changes in the behaviour of Stagnicola elodes associated with the emergence of Plagiorchis elegans cercariae. Within 15 min of the reduction in light intensity, which triggered the onset of cercarial emergence, infected snails moved to the top of the water column and remained there for 2-3 h. Seventy-n...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000077775

    authors: Lowenberger CA,Rau ME

    更新日期:1994-07-01 00:00:00

  • Molecular study of Echinococcus in west-central China.

    abstract::West-central China is an important endemic focus of both alveolar and cystic echinococcosis where several species of intermediate host are commonly infected with Echinococcus granulosus and E. multilocularis . Isolates of E. granulosus were collected from humans and other animals from different geographical areas of Q...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005007973

    authors: Yang YR,Rosenzvit MC,Zhang LH,Zhang JZ,McManus DP

    更新日期:2005-10-01 00:00:00

  • Rates and intensity of re-infection with human helminths after treatment and the influence of individual, household, and environmental factors in a Brazilian community.

    abstract::This study quantifies the rate and intensity of re-infection with human hookworm and Schistosoma mansoni infection 12 months following successful treatment, and investigates the influence of socio-economic, geographical and environmental factors. A longitudinal study of 642 individuals aged over 5 years was conducted ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011001132

    authors: Cundill B,Alexander N,Bethony JM,Diemert D,Pullan RL,Brooker S

    更新日期:2011-09-01 00:00:00

  • Inhibition by anti-malarial drugs of haemoglobin denaturation and iron release in acidified red blood cell lysates--a possible mechanism of their anti-malarial effect?

    abstract::Intraerythrocytic malaria parasites ingest the cytosol of their host cell and digest it inside their acid food vacuoles. Acidified (pH 4-5.5, 37 degrees C) human red blood cell lysates were used to simulate this process, measuring the denaturation of haemoglobin (Hb) and the release of iron, in the absence or presence...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000075910

    authors: Gabay T,Krugliak M,Shalmiev G,Ginsburg H

    更新日期:1994-05-01 00:00:00

  • Parasite biodiversity and its determinants in coastal marine teleost fishes of Brazil.

    abstract::Recent studies of the forces behind the diversification of parasite assemblages have shed light on many aspects of parasite biodiversity. By using only parasite species richness as their measure of diversity, however, previous investigations have ignored the relatedness among parasite species and the taxonomic structu...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004005050

    authors: Luque JL,Mouillot D,Poulin R

    更新日期:2004-06-01 00:00:00

  • Fitness and virulence of a bacterial endoparasite in an environmentally stressed crustacean host.

    abstract::Host-parasite interactions are shaped by the co-evolutionary arms race of parasite virulence, transmission success as well as host resistance and recovery. The virulence and fitness of parasites may depend on host condition, which is mediated, for instance, by host energy constraints. Here, we investigated to what ext...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010000995

    authors: Coors A,De Meester L

    更新日期:2011-01-01 00:00:00

  • An agent-based model of exposure to human toxocariasis: a multi-country validation.

    abstract::Seroprevalence data illustrate that human exposure to Toxocara is frequent. Environmental contamination with Toxocara spp. eggs is assumed to be the best indicator of human exposure, but increased risk of exposure has also been associated with many other factors. Reported associations are inconsistent, however, and th...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182013000310

    authors: Kanobana K,Devleesschauwer B,Polman K,Speybroeck N

    更新日期:2013-07-01 00:00:00

  • gamma delta T cells do not play a major role in controlling infection in experimental cysticercosis.

    abstract::Protective immunity against larval Taenia crassiceps has been shown to rely on T cells; however, the roles of the specific subsets of T cells during infection are not known. To investigate a possible role for gamma delta T cells, this study investigated larval infection in delta-chain knock-out C57BL/6 (deltaKO) and w...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099004771

    authors: Toenjes SA,Spolski RJ,Mooney KA,Kuhn RE

    更新日期:1999-10-01 00:00:00

  • Advances and challenges in barcoding of microbes, parasites, and their vectors and reservoirs.

    abstract::DNA barcoding is now a common tool in parasitology and epidemiology, which require good methods for identification not only of parasites and pathogens but vectors and reservoirs. This special issue presents some advances and challenges in barcoding of microbes, parasites, and their vectors and reservoirs. DNA barcodin...

    journal_title:Parasitology

    pub_type: 社论

    doi:10.1017/S0031182018000884

    authors: Morand S

    更新日期:2018-04-01 00:00:00

  • Heterogeneity of metalloprotease expression in Trypanosoma cruzi.

    abstract::Expression of metalloprotease activities during metacyclogenesis of a series of strains and clones of Trypanosoma cruzi was investigated using SDS-polyacrylamide gel electrophoresis with a range of different co-polymerized proteolytic substrates. A complex pattern of metalloprotease expression, with considerable quant...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000066622

    authors: Lowndes CM,Bonaldo MC,Thomaz N,Goldenberg S

    更新日期:1996-04-01 00:00:00

  • Neospora caninum cytoplasmic dynein LC8 light chain 2 (NcDYNLL2) is differentially produced by pathogenically distinct isolates and regulates the host immune response.

    abstract::Neospora caninum is the causative agent of bovine neosporosis. A N. caninum cytoplasmic dynein LC8 light chain (NcDYNLL) protein was characterized in this study. Cytoplasmic dyneins, including DYNLLs, belong to the microtubule minus-end-directed motor proteins and are involved in many cellular processes. Previous micr...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201800207X

    authors: Cao L,Fetterer R,Qu G,Zhang X,Tuo W

    更新日期:2018-12-18 00:00:00

  • Eimeria species which infect the chicken contain virus-like RNA molecules.

    abstract::There is increasing support for the presence of viruses and virus-like particles inside protozoan cells. This study describes viral-like RNA molecules that have been detected in two species of Eimeria that infect the chicken. The RNA molecule identified in E. maxima has been characterized: subcellular fractionation st...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063198

    authors: Ellis J,Revets H

    更新日期:1990-10-01 00:00:00

  • Sub-cellular fractionation of Trypanosoma brucei. Isolation and characterization of plasma membranes.

    abstract::A procedure is described for the isolation of sub-cellular fractions from bloodstream forms of Trypanosoma brucei. The method leaves intact most of the nuclei, mitochondria and microbodies. All the fractions have been chemically characterized and tested for 10 enzymatic markers. About 5% of total cell protein was isol...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000000974

    authors: Rovis L,Baekkeskov S

    更新日期:1980-06-01 00:00:00

  • Tegumental disruption with Triton X-100 and its effects on longitudinal muscle function in male Schistosoma mansoni.

    abstract::Longitudinal muscles in adult male Schistosoma mansoni appear to remain in almost all respects functional after tegumental disruption by Triton X-100. Responses to high K+, ouabain, low temperature, praziquantel and neurotransmitters are still present. Muscle membrane potentials remain near those of control animals an...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000052422

    authors: Depenbusch JW,Thompson DP,Pax RA,Bennett JL

    更新日期:1983-08-01 00:00:00

  • Mutual dilution of infection by an introduced parasite in native and invasive stream fishes across Hawaii.

    abstract::The presence of introduced hosts can increase or decrease infections of co-introduced parasites in native species of conservation concern. In this study, we compared parasite abundance, intensity, and prevalence between native Awaous stamineus and introduced poeciliid fishes by a co-introduced nematode parasite (Camal...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001001

    authors: Gagne RB,Heins DC,McIntyre PB,Gilliam JF,Blum MJ

    更新日期:2016-10-01 00:00:00

  • High diversity of fish ectoparasitic monogeneans (Dactylogyrus) in the Iberian Peninsula: a case of adaptive radiation?

    abstract::The epicontinental fauna of the Iberian Peninsula is strongly influenced by its geographical history. As the possibilities for dispersion of organisms into and from this region were (and still are) limited, the local fauna consists almost exclusively of endemic species. Almost all Iberian freshwater fishes of the fami...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020000050

    authors: Benovics M,Desdevises Y,Šanda R,Vukić J,Scheifler M,Doadrio I,Sousa-Santos C,Šimková A

    更新日期:2020-04-01 00:00:00

  • Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism.

    abstract::Isolates of Trypanosoma cruzi from human patients, domestic and sylvatic animals and vector insects were obtained in different areas of Argentina. Electrophoretic patterns of enzymes from extracts of 95 isolates were analysed. On the basis of zymograms providing information on 10 loci, 12 zymodemes are described accor...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000067755

    authors: de Luca D'oro GM,Gardenal CN,Perret B,Crisci JV,Montamat EE

    更新日期:1993-11-01 00:00:00

  • An ELISA for the detection of pentastomid infections in the rat.

    abstract::A specific enzyme-linked immunosorbent assay, based on a 48 kDa metallo-proteinase isolated from the frontal glands of a porocephalid pentastomid (Porocephalus crotali) in rat intermediate hosts, is described. SDS-PAGE analysis of whole nymphal homogenate, followed by Western blotting and probing with immune rat serum...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000059849

    authors: Jones DA,Riley J

    更新日期:1991-12-01 00:00:00

  • Modulation of immune responses to parasitoids by polydnaviruses.

    abstract::Parasitoids are parasites that invariably kill their host. Polydnaviruses are injected by parasitoid wasps into the body cavity of their insect host and cause immunosuppression, allowing the parasitoid to develop in the absence of encapsulation. One of the targets of the polydnaviruses are the haemocytes of the host, ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182000084948

    authors: Beckage NE

    更新日期:1998-01-01 00:00:00

  • The development of Haemonchus contortus, a nematode parasite of the ovine abomasum, in the laboratory rabbit.

    abstract::The development and growth of Haemonchus contortus was studied in the stomach of the laboratory rabbit. Less than 1% of the ensheathed infective larvae given to rabbits became established, whereas after exsheathment in vitro under physiological conditions, 20-30% of the larvae administered developed to the 4th stage. ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000047867

    authors: Mapes CJ,Gallie GJ

    更新日期:1977-06-01 00:00:00

  • Dipstick dot ELISA for the detection of Taenia coproantigens in humans.

    abstract::A dipstick dot ELISA for detection of Taenia-specific coproantigens was developed. The test was based on a sandwich ELISA using antibodies raised against adult Taenia solium. Antibodies were absorbed to nitrocellulose paper previously adhered to acetate plastic to form dipsticks. Once blocked with 5% skimmed milk and ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000079439

    authors: Allan JC,Mencos F,Garcia-Noval J,Sarti E,Flisser A,Wang Y,Liu D,Craig PS

    更新日期:1993-07-01 00:00:00

  • A novel duplex ddPCR assay for the diagnosis of schistosomiasis japonica: proof of concept in an experimental mouse model.

    abstract::The current World Health Organization strategic plan targets the elimination of schistosomiasis as a public health problem by 2025 and accurate diagnostics will play a pivotal role in achieving this goal. DNA-based detection methods provide a viable alternative to some of the commonly used tests, notably microscopy an...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201700021X

    authors: Weerakoon KG,Gordon CA,Cai P,Gobert GN,Duke M,Williams GM,McManus DP

    更新日期:2017-07-01 00:00:00