Abstract:
:Neospora caninum is the causative agent of bovine neosporosis. A N. caninum cytoplasmic dynein LC8 light chain (NcDYNLL) protein was characterized in this study. Cytoplasmic dyneins, including DYNLLs, belong to the microtubule minus-end-directed motor proteins and are involved in many cellular processes. Previous microarray studies revealed that NcDYNLL was downregulated in the non-pathogenic clone, Ncts-8, when compared with the wild-type NC1 isolate. The present study showed that DYNLLs from different species are highly conserved (>85% identity), and the NcDYNLL belongs to the DYNLL2 family. NcDYNLL2 and Toxoplasma gondii DYNLL2 have identical amino acid sequences, although they are slightly divergent at the genetic level (89% identity). NcDYNLL2 was cloned and expressed in Escherichia coli and purified. NcDYNLL2 was identified in soluble and insoluble fractions of tachyzoite lysate. As expected, soluble NcDYNLL2 was lower in the Ncts-8 lysate when compared with that of NC1 isolate. NcDYNLL2 release by the tachyzoites was low; however, it was increased when tachyzoites were treated with either calcium ionophore or ethanol. The data indicate that NcDYNLL2 may be actively secreted at low levels, but the secretion was upregulated by agents that also augment microneme protein secretions. Immunostaining of NcDYNLL2 in isolated and intracellular Neospora tachyzoites showed a diffuse distribution pattern. Furthermore, rNcDYNLL2 was internalized by the host immune cells and stimulated tumour necrosis factor-α) and interleukin-12 (IL-12) production by murine dendritic cells. Taken together, these results suggest that NcDYNLL2 is a secretory protein that cross-regulates host immunity.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Cao L,Fetterer R,Qu G,Zhang X,Tuo Wdoi
10.1017/S003118201800207Xsubject
Has Abstractpub_date
2018-12-18 00:00:00pages
1-8eissn
0031-1820issn
1469-8161pii
S003118201800207Xpub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::The anticoccidial effect of narasin against Eimeria flavescens, E. intestinalis, E. magna, E. perforans and E. stiedai was tested in experimentally infected rabbits. The drug was highly effective in reducing oocyst output and in preventing clinical signs. Maximum activity as measured by oocyst discharge was achieved w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000085309
更新日期:1981-10-01 00:00:00
abstract::The snail Bulinus nasutus 1214 possesses a potent haemagglutinin (end-point titre with human erythrocytes, 2(-18)) in its cell-free haemolymph which also binds to the miracidia (but not other larvae) of the incompatible parasite Schistosoma margrebowiei. We have purified a protein possessing this haemagglutinating pro...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000074928
更新日期:1993-02-01 00:00:00
abstract::Neospora caninum is an Apicomplexan protozoan that has the dog as a definitive host and cattle (among other animals) as intermediate hosts. It causes encephalopathy in dogs and abortion in cows, with significant loss in worldwide livestock. As any Apicomplexan, the parasite invades the cells using proteins contained i...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182010001290
更新日期:2011-03-01 00:00:00
abstract::Passive transfer experiments showed that 76% of the resistance induced in CBA/Ca mice by exposure to radiation-attenuated cercariae of Schistosoma mansoni could be transferred to naive recipients by administration of donor serum. The level of protection achieved depended on the volume of serum administered and immunit...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000058492
更新日期:1988-10-01 00:00:00
abstract::The in vivo activity of two cyclosporins, cyclosporin A (CsA) and a non-immunosuppressive derivative of dihydrocylosporin A (DHCsA-d) against three tapeworms, Hymenolepis microstoma, H. diminuta and Mesocestoides corti, has been assessed. CsA reversibly reduced the dry weight of H. microstoma in the mouse, briefly del...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000062211
更新日期:1989-04-01 00:00:00
abstract::SUMMARY The necessity to easily establish Histomonas meleagridis cultures has been underlined extensively by many researchers in order to gain more insights in the biology of H. meleagridis. In addition the occurrence of different protozoa in the caeca of birds impedes, however, the isolation and propagation of H. mel...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013000723
更新日期:2013-09-01 00:00:00
abstract::Endotoxaemia has been described in cases of Human African trypanosomiasis (HAT), but it is unclear if this phenomenon influences inflammatory pathology either in the periphery or central nervous system (CNS). We studied endotoxin concentrations in the plasma and cerebrospinal fluid (CSF) of Trypanosoma brucei rhodesie...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016002158
更新日期:2017-04-01 00:00:00
abstract::Polychromophilus murinus, a malaria parasite of Chiroptera is reported from Myotis daubentoni in England. The vector was suspected to be the ectoparasitic Nycteribiid fly, Nycteribia kolenatii. N. kolenatii collected from wild-caught M. daubentoni were found to have oocysts on the midgut and sporozoites in the salivar...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000080215
更新日期:1988-06-01 00:00:00
abstract::Acute Chagas disease outbreaks are related to the consumption of food or drink contaminated by triatomine feces, thus making oral infection an important route of transmission. Both vector-borne and oral infections trigger important cardiac manifestations in the host that are related to a dysregulated immune response. ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013002059
更新日期:2014-06-01 00:00:00
abstract::The World Health Organization announced in November 2014 at the fourth international meeting on 'the control of neglected zoonotic diseases - from advocacy to action', that intervention tools for eliminating Taenia solium taeniosis/cysticercosis (TSTC) are in place. The aim of this work was to elucidate theoretical ou...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000500
更新日期:2017-01-01 00:00:00
abstract::Multiple parasitic infections have become increasingly recognized as a result of improvements in laboratory diagnosis and a growing population of immunocompromised individuals. This review examines the principles of chemotherapy in groupings of multiple infections which are of particular clinical significance. ...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000017674
更新日期:2001-01-01 00:00:00
abstract::A new model for the study of granuloma formation in the liver is described. Rats received an injection of 20,000 Schistosoma mansoni eggs in the portal vein and granuloma formation was evaluated at 3, 5 and 7 weeks post-injection. Liver collagen was estimated at the same time and serum procollagen III peptide, a marke...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060339
更新日期:1991-02-01 00:00:00
abstract::To test the homogeneity of 18S sequences within Trypanosoma cruzi, riboprint profiles were separated by temperature gradient gel electrophoresis (TGGE). Upon interpretation of melting curves of fragments within a riboprint profile, there appeared to be two 18S sequence types within each stock examined. Two similar typ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098002972
更新日期:1998-09-01 00:00:00
abstract::Two cDNAs encoding cysteine proteinases were isolated from a cDNA library constructed from feeding females of Heterodera glycines. The library was screened with a cysteine proteinase gene fragment originally amplified from cDNA of H. glycines. Database searches predict that 1 cDNA (hgcp-I) encodes a cathepsin L-like p...
journal_title:Parasitology
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::By means of conventional light microscopical and counting procedures, estimations have been made of the average number and size of ovarian balls from unfertilized and fertilized female Moniliformis dubius. The observations were made on worms recovered from experimental primary infections lasting 1, 2, 3, 4, 5, 8, 11, ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000051337
更新日期:1976-08-01 00:00:00
abstract::Since 2004 there has been a remarkable increment in our knowledge of the proteins and glycans that reside at, or are released from the surfaces of schistosomes in the mammalian host. Initial characterization of the soluble proteome permits distinctions to be made between the parasite secretome and its necrotome. The p...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182012000339
更新日期:2012-08-01 00:00:00
abstract::Merocyanin 540 (Mc540) is a fluorescent compound which is thought to bind to membranes in which there are substantial amounts of lipid in the lipid-crystalline phase. It is shown here to be of value in detecting the transformation by both mechanical and skin-penetration methods of the cercaria to the schistosomulum. T...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000081671
更新日期:1988-02-01 00:00:00
abstract::Dr David Livingstone died on May 1st 1873. He was 60 years old and had spent much of the previous 30 years walking across large stretches of Southern Africa, exploring the terrain he hoped could provide new environments in which Europeans and Africans could cohabit on equal terms and bring prosperity to a part of the ...
journal_title:Parasitology
pub_type: 传,历史文章,杂志文章
doi:10.1017/S003118201600202X
更新日期:2017-10-01 00:00:00
abstract::Epidemiological rate parameters of host generalist parasites are difficult to estimate, especially in cases where variation in parasite performance can be attributed to host species. Such cases are likely common for generalist parasites of sympatric grazing mammals. In this study, we combined data from experimental ex...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015000621
更新日期:2015-09-01 00:00:00
abstract::Infection of breeding hens with Eimeria maxima induces production of Eimeria-specific IgG antibodies which are transferred to hatchlings via the egg yolk and confer a high degree of maternal immunity against homologous challenge and partial immunity to infection with another important species, Eimeria tenella. As an e...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076423
更新日期:1994-12-01 00:00:00
abstract::Many clinically useful antibacterial drugs have intracellular target sites. Therefore, in order to reach their targets, these compounds must be able to cross bacterial outer and cytoplasmic membranes. Considerable information is available on the mechanisms by which antibiotics cross bacterial membranes and, in many ca...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000085966
更新日期:1988-01-01 00:00:00
abstract::Ultrastructural investigations (SEM, TEM) combined with lectin-binding analysis, have revealed concurrent modifications in tegumentary structure and surface glycoconjugates during the establishment and differentiation of Echinococcus multilocularis metacestodes in jirds. The laminated layer, which is amorphous and ric...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200006087x
更新日期:1992-02-01 00:00:00
abstract::Trypanosoma brucei is the causative agent of African sleeping sickness in humans and one of several pathogens that cause the related veterinary disease Nagana. A complex co-evolution has occurred between these parasites and primates that led to the emergence of trypanosome-specific defences and counter-measures. The f...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182014000602
更新日期:2015-02-01 00:00:00
abstract::Digenean parasites infecting four Cominella whelk species (C. glandiformis, C. adspersa, C. maculosa and C. virgata), which inhabit New Zealand's intertidal zone, were analysed using molecular techniques. Mitochondrial 16S and cytochrome oxidase 1 (COI) and nuclear rDNA ITS1 sequences were used to infer phylogenetic r...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000494
更新日期:2016-08-01 00:00:00
abstract::A specific enzyme-linked immunosorbent assay, based on a 48 kDa metallo-proteinase isolated from the frontal glands of a porocephalid pentastomid (Porocephalus crotali) in rat intermediate hosts, is described. SDS-PAGE analysis of whole nymphal homogenate, followed by Western blotting and probing with immune rat serum...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000059849
更新日期:1991-12-01 00:00:00
abstract::The complete nucleotide sequence of the mitochondrial genome was determined for the fish tapeworm Diphyllobothrium latum. This genome is 13,608 bp in length and encodes 12 protein-coding genes (but lacks the atp8), 22 transfer RNA (tRNA) and 2 ribosomal RNA (rRNA) genes, corresponding to the gene complement found thus...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118200600206X
更新日期:2007-05-01 00:00:00
abstract::The host-parasite relationship is exquisitely specific. In exploiting the host niche, a variety of helminth parasites have been shown to directly manipulate their hosts' immune responses. We assessed the ability of a whole-worm extract of Hymenolepis diminuta to modulate immune cell activation. Immune cells isolated f...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006985
更新日期:2005-05-01 00:00:00
abstract::The host recognition and invasion process of Myxobolus cerebralis actinospores (triactinomyxon, TAM) was studied on a genetic level. A small-scale in vitro assay was developed to activate a large number of TAMs simultaneously, and to monitor the host invasion in the absence of live fish. The transcriptomes of non-acti...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008005398
更新日期:2009-04-01 00:00:00
abstract::Virulence factors from the ROP2-family have been extensively studied in Toxoplasma gondii, but in the closely related Neospora caninum only NcROP2Fam-1 has been partially characterized to date. NcROP40 is a member of this family and was found to be more abundantly expressed in virulent isolates. Both NcROP2Fam-1 and N...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015001511
更新日期:2016-01-01 00:00:00
abstract::The biodiversity of Trypanosoma cruzi is one of the main factors complicating the understanding of its molecular epidemiology. As an alternative to classical genetic methods, investigators have used DNA-mediated transformation techniques to study this diversity. Recently, transient expression data were shown to correl...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099005053
更新日期:1999-12-01 00:00:00