Identification of an antigen of Onchocerca volvulus of possible diagnostic use.

Abstract:

:Adult Onchocerca volvulus worms obtained by enzyme digestion from nodules of infected Mexicans were radio-isotope labelled by the chloramine-T or Bolton-Hunter methods. No antigenic determinants were detected in extracts of worms labelled by the chloramine-T method but 3 antigens were detected in extracts of the Bolton-Hunter labelled worms. Two were present in such small amounts that it was impractical to investigate them further, but a major component of mol. wt 20 kDa was purified by gel filtration and used in a serological survey of inhabitants of villages in Southern Mexico. Using the 20 kDa antigen, which is superficially located on both sexes of O. volvulus, sera from both non-endemic and endemic regions were analysed by radio-immunoprecipitation of this antigen. In Southern Mexico, the average sensitivity of the test was 92%, and the specificity 98%. Whilst the 20 kDa antigen did not detect antibodies in the sera of Trinidadians infected with Wuncheria bancrofti or Mansonella ozzardi, this antigen detected high levels of antibodies in Indians exposed to W. bancrofti.

journal_name

Parasitology

journal_title

Parasitology

authors

Philipp M,Gómez-Priego A,Parkhouse RM,Davies MW,Clark NW,Ogilvie BM,Beltrán-Hernández F

doi

10.1017/s0031182000001323

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

295-309

eissn

0031-1820

issn

1469-8161

journal_volume

89 ( Pt 2)

pub_type

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