Interferon-gamma-induced apoptosis in host cells infected with Neospora caninum.

Abstract:

:Interferon-gamma (IFN-gamma) has a crucial role for host defence against parasite infection. It is not clear, however, how IFN-gamma affects the parasite-infected host cells. The effect of IFN-gamma on Neospora caninum-infected cells was investigated in murine fibroblasts and canine kidney cells in vitro. In the presence of IFN-gamma, the viability of the infected host cell was decreased and apoptotic cell death occurred, as analysed by DNA stainings with propidium iodide and a terminal deoxy-nucleotidyltransferase-mediated dUTP-biotin nick end labelling (TUNEL) and DNA fragmentation. The percentage of apoptotic cells depended on the dose of IFN-gamma. Flow cytometric analysis indicated a significant increase of FasL expression on the IFN-gamma treated cells following N. caninum infection. Moreover, IFN-gamma treatment down-regulated Bcl-2 expression in the cells cultured with N. caninum while parasite infection up-regulated Bcl-2 expression. The present study suggests that the IFN-gamma induced increases of FasL expression and down-regulated Bcl-2 expression in N. caninum-infected cells are associated with apoptosis in vitro.

journal_name

Parasitology

journal_title

Parasitology

authors

Nishikawa Y,Mishima M,Nagasawa H,Igarashi I,Fujisaki K,Otsuka H,Mikami T

doi

10.1017/s0031182001008095

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

25-31

issue

Pt 1

eissn

0031-1820

issn

1469-8161

journal_volume

123

pub_type

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