Abstract:
:Interferon-gamma (IFN-gamma) has a crucial role for host defence against parasite infection. It is not clear, however, how IFN-gamma affects the parasite-infected host cells. The effect of IFN-gamma on Neospora caninum-infected cells was investigated in murine fibroblasts and canine kidney cells in vitro. In the presence of IFN-gamma, the viability of the infected host cell was decreased and apoptotic cell death occurred, as analysed by DNA stainings with propidium iodide and a terminal deoxy-nucleotidyltransferase-mediated dUTP-biotin nick end labelling (TUNEL) and DNA fragmentation. The percentage of apoptotic cells depended on the dose of IFN-gamma. Flow cytometric analysis indicated a significant increase of FasL expression on the IFN-gamma treated cells following N. caninum infection. Moreover, IFN-gamma treatment down-regulated Bcl-2 expression in the cells cultured with N. caninum while parasite infection up-regulated Bcl-2 expression. The present study suggests that the IFN-gamma induced increases of FasL expression and down-regulated Bcl-2 expression in N. caninum-infected cells are associated with apoptosis in vitro.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Nishikawa Y,Mishima M,Nagasawa H,Igarashi I,Fujisaki K,Otsuka H,Mikami Tdoi
10.1017/s0031182001008095subject
Has Abstractpub_date
2001-07-01 00:00:00pages
25-31issue
Pt 1eissn
0031-1820issn
1469-8161journal_volume
123pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::The rapid elimination of the intestinal phase of Trichinella spiralis in NIH mice is associated with progressive inflammation of the intestinal tract. The non-specific effects of this inflammation were studied in mice concurrently infected with an unrelated parasite, Hymenolepis diminuta, which does not stimulate a vi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000048344
更新日期:1977-08-01 00:00:00
abstract::Thirty-one stocks of Trypanosoma cruzi, the agent of Chagas disease, representative of the genetic variability of the 2 principal lineages, that subdivide T. cruzi, were selected on the basis of previous multilocus enzyme electrophoresis analysis using 21 loci. Analyses were performed with lower numbers of loci to exp...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182003003573
更新日期:2003-09-01 00:00:00
abstract::DNA barcoding is now a common tool in parasitology and epidemiology, which require good methods for identification not only of parasites and pathogens but vectors and reservoirs. This special issue presents some advances and challenges in barcoding of microbes, parasites, and their vectors and reservoirs. DNA barcodin...
journal_title:Parasitology
pub_type: 社论
doi:10.1017/S0031182018000884
更新日期:2018-04-01 00:00:00
abstract::Myxozoans have been successfully used as tags for fish stock identification around the world. However, few studies using myxozoan tags have been carried out in the Southern Atlantic, a region with complex oceanography that constitutes a potentially suitable scenario for testing the utility of myxozoans as indicators. ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000226
更新日期:2016-05-01 00:00:00
abstract::Cutaneous Leishmaniasis (CL) is considered a neglected tropical disease which in Pakistan can now be considered as a growing public health problem. The exact figures on the magnitude of the disease are lacking both at the national and regional level and only a few health centres are available for diagnosis of CL. The ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182020002413
更新日期:2020-12-23 00:00:00
abstract::Infectious agents have considerable potential to regulate or constrain the population growth of vertebrate hosts in natural habitats. A broad theoretical framework provides many insights into how the biology of the parasite and the demography of the host interact to determine this impact. It may manifest itself as a s...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s003118200007579x
更新日期:1995-01-01 00:00:00
abstract::We describe a laboratory model using Eimeria falciformis and E. pragensis to investigate some of the interactions in double-species infections of Eimeria. Mice were given trickle infections by oral inoculation of 100 sporulated oocysts of one species at 3 or 4 day intervals throughout the experiments and, once immunit...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098003308
更新日期:1998-11-01 00:00:00
abstract::Ocular, peroral, intraperitoneal, intramuscular, and subcutaneous inoculation of severe combined immunodeficient (SCID) mice with spores of the human isolate (CDC: V404) of Brachiola algerae (syn. Nosema algerae) (Phylum Microspora) revealed that the microsporidium develops in viscera of the immunodeficient mouse host...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001008228
更新日期:2001-08-01 00:00:00
abstract::Multiple infections, those by more than one parasite in the same erythrocyte, may be adaptive for the malarial parasite as a means to ensure fertility. Alternatively they may simply be the consequence of a non-adaptive process forcing several parasites to compete for resources in one host cell. Avian hosts infected wi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008711
更新日期:2006-01-01 00:00:00
abstract::Intact, viable adults, infective and newborn larvae of Trichinella pseudospiralis were surface labelled with 125I by the chloramine T method and labelled proteins were compared with those obtained from equivalent stages of T. spiralis. Electron-microscope autoradiography determined that labelled proteins were restrict...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200004991x
更新日期:1986-08-01 00:00:00
abstract::Seroprevalence data illustrate that human exposure to Toxocara is frequent. Environmental contamination with Toxocara spp. eggs is assumed to be the best indicator of human exposure, but increased risk of exposure has also been associated with many other factors. Reported associations are inconsistent, however, and th...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013000310
更新日期:2013-07-01 00:00:00
abstract::A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 10(4)-10(5) promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 10(6) cells of...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000073650
更新日期:1992-08-01 00:00:00
abstract::Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process of Ascaris suum larvae. This study describes moulting and development-specific protein expression profiles of A. suum lung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepa...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006000576
更新日期:2006-10-01 00:00:00
abstract::In an interconnected world, the international pet trade on wild animals is becoming increasingly important. As a consequence, non-native parasite species are introduced, which affect the health of wildlife and contribute to the loss of biodiversity. Because the investigation of parasite diversity within vulnerable hos...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016002183
更新日期:2017-04-01 00:00:00
abstract::In order to investigate the phenomenon of age-related immunity to visceral leishmaniasis, a 1 year prospective survey was carried out on 5671 people in a Leishmania infantum focus in north-west Iran. The average incidence rate of infection since 1985 was 2.8%/year with all ages equally at risk. One in 13 infections in...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099004680
更新日期:1999-09-01 00:00:00
abstract::Infection of breeding hens with Eimeria maxima induces production of Eimeria-specific IgG antibodies which are transferred to hatchlings via the egg yolk and confer a high degree of maternal immunity against homologous challenge and partial immunity to infection with another important species, Eimeria tenella. As an e...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076423
更新日期:1994-12-01 00:00:00
abstract::Virulence factors from the ROP2-family have been extensively studied in Toxoplasma gondii, but in the closely related Neospora caninum only NcROP2Fam-1 has been partially characterized to date. NcROP40 is a member of this family and was found to be more abundantly expressed in virulent isolates. Both NcROP2Fam-1 and N...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015001511
更新日期:2016-01-01 00:00:00
abstract::IgA and IgE activity against Teladorsagia circumcincta was investigated in a flock of Texel lambs following natural, mixed nematode infection among lambs. The distribution of IgA activity was similar to a gamma distribution whereas IgE activity was different. Box-Cox analysis demonstrated that X0.25 was a suitable tra...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182010000028
更新日期:2010-07-01 00:00:00
abstract::Protective immunity against larval Taenia crassiceps has been shown to rely on T cells; however, the roles of the specific subsets of T cells during infection are not known. To investigate a possible role for gamma delta T cells, this study investigated larval infection in delta-chain knock-out C57BL/6 (deltaKO) and w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099004771
更新日期:1999-10-01 00:00:00
abstract::In areas where malaria is endemic, helminthic infections, caused by intestinal or filarial parasites, commonly coexist with malaria in the same individual. This study investigates the course of Plasmodium berghei malaria infection in CBA/J mice inoculated with irradiated attenuated 3rd-stage larvae (L3) of Brugia paha...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182096008566
更新日期:1997-04-01 00:00:00
abstract::The phenotypic activity of two 5-nitroindazolinones, i.e. 2-benzyl-1-propyl (22) and 2-benzyl-1-butyl (24) derivatives, previously proposed as anti-Trypanosoma cruzi prototypes, was presently assayed on bloodstream trypomastigotes (BT) of the moderately drug-resistant Y strain. Further exploration of putative targets ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001098
更新日期:2016-09-01 00:00:00
abstract::Here, the validity of the assumption of concerted evolution of ribosomal regions in larval and adult Cooperia oncophora was assessed. In each of 4 individuals of this parasitic nematode, at least 78% of the sequences comprised different ITS variants. This implies that concerted evolution is not acting, which is corrob...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004005499
更新日期:2004-08-01 00:00:00
abstract::Serology is an important tool for the diagnosis of alveolar echinococcosis (AE) in humans. In order to improve serodiagnostic performance, we have developed an in vitro-produced Echinococcus mulilocularis metacestode vesicle fluid (EmVF) antigen for application in an immunoblot assay. Immunoblot analysis of EmVF revea...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006002083
更新日期:2007-06-01 00:00:00
abstract::We have cloned a cDNA encoding a novel antigen from a Sarcoptes scabiei (Acari) cDNA library by immunoscreening with sera from S. scabiei-infected dogs. The antigen is encoded by a 2,157 bp mRNA with a predicted open reading frame of 719 amino acids (molecular weight 79 kDa). Our sequence analysis identified the prese...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008747
更新日期:2006-01-01 00:00:00
abstract::An egg-adapted line of Eimeria necatrix has now been passaged 40 times in the chorio-allantoic membranes of embryonated eggs. Between the 21st and 40th passage in eggs the parasite was subjected to a selection for precocious development, and its pre-patent period in this host is now 123-125 h. The parasite continued t...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000044784
更新日期:1982-04-01 00:00:00
abstract::Avian malaria is a mosquito-borne disease caused by Plasmodium spp. protozoa. Although these parasites have been extensively studied in North America and Eurasia, knowledge on the diversity of Plasmodium, its vectors and avian hosts in Africa is scarce. In this study, we report on natural malarial infections in free-r...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182020000566
更新日期:2020-08-01 00:00:00
abstract::Sporulated oocysts or oocyst hulls of Eimeria acervulina given via the crop, and oocyst juice or sporozoites inoculated directly into the intestinal lumen, were tested for their effects on the hypersensitivity of the intestines of immunized and susceptible birds. Only viable sporozoites consistently caused an increase...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000047132
更新日期:1975-12-01 00:00:00
abstract::Angiostrongylus cantonensis is a metastrongyloid nematode found widely in the Asia-Pacific region, and the aetiological agent of angiostrongyliasis; a disease characterized by eosinophilic meningitis. Rattus rats are definitive hosts of A. cantonensis, while intermediate hosts include terrestrial and aquatic molluscs....
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182016000652
更新日期:2016-08-01 00:00:00
abstract::Recent studies of Echinococcus granulosus molecular strain typing have enabled a better understanding of the transmission cycle of cystic echinococcosis. There have been many publications in this area but there is a need for the evaluation of these tools. We have attempted to respond to this need in our study, which a...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182003004359
更新日期:2004-02-01 00:00:00
abstract::Insecticide-treated nets (ITNs) are a major tool to control malaria. Over recent years increased ITN coverage has been associated with decreased malaria transmission. However, ITN 'misuse' has been increasingly reported and whether this emergent behaviour poses a threat to successful malaria control and elimination is...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182012002077
更新日期:2013-04-01 00:00:00