Echinococcus granulosus strain typing in North Africa: comparison of eight nuclear and mitochondrial DNA fragments.

Abstract:

:Recent studies of Echinococcus granulosus molecular strain typing have enabled a better understanding of the transmission cycle of cystic echinococcosis. There have been many publications in this area but there is a need for the evaluation of these tools. We have attempted to respond to this need in our study, which assessed 8 DNA fragments of 40 E. granulosus cysts from North Africa. Parasitological material was collected from 5 types of intermediate hosts, in 5 different countries. The primers chosen to amplify DNA targets were defined either in nuclear DNA, or in mitochondrial DNA. After amplification, PCR products were sequenced. The sequences obtained were aligned and comparisons were made within the group and with GenBank sequences. Whether the target was nuclear or mitochondrial, the same 2 main groups of genotypes were found. The first one, the 'sheep' strain, was found in the human, sheep and cattle samples collected in North Africa. The second one, the 'camel' strain, was found in the camel cysts and cattle and human cysts from Mauritania. These findings further confirm the congruence of the data given by the nuclear and the mitochondrial genome.

journal_name

Parasitology

journal_title

Parasitology

authors

Bart JM,Bardonnet K,Elfegoun MC,Dumon H,Dia L,Vuitton DA,Piarroux R

doi

10.1017/s0031182003004359

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

229-34

issue

Pt 2

eissn

0031-1820

issn

1469-8161

journal_volume

128

pub_type

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