Abstract:
:Recent studies of Echinococcus granulosus molecular strain typing have enabled a better understanding of the transmission cycle of cystic echinococcosis. There have been many publications in this area but there is a need for the evaluation of these tools. We have attempted to respond to this need in our study, which assessed 8 DNA fragments of 40 E. granulosus cysts from North Africa. Parasitological material was collected from 5 types of intermediate hosts, in 5 different countries. The primers chosen to amplify DNA targets were defined either in nuclear DNA, or in mitochondrial DNA. After amplification, PCR products were sequenced. The sequences obtained were aligned and comparisons were made within the group and with GenBank sequences. Whether the target was nuclear or mitochondrial, the same 2 main groups of genotypes were found. The first one, the 'sheep' strain, was found in the human, sheep and cattle samples collected in North Africa. The second one, the 'camel' strain, was found in the camel cysts and cattle and human cysts from Mauritania. These findings further confirm the congruence of the data given by the nuclear and the mitochondrial genome.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Bart JM,Bardonnet K,Elfegoun MC,Dumon H,Dia L,Vuitton DA,Piarroux Rdoi
10.1017/s0031182003004359subject
Has Abstractpub_date
2004-02-01 00:00:00pages
229-34issue
Pt 2eissn
0031-1820issn
1469-8161journal_volume
128pub_type
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