The intestinal milieu influences the immunoproteome of male and female Heligmosomoides polygyrus bakeri L4 stage.

Abstract:

:The gastrointestinal nematode Heligmosomoides polygyrus bakeri shows enhanced survival in mice with colitis. As the antibody response plays an important role in antiparasitic immunity, antibodies against male and female L4 H. polygyrus were examined in mice with and without colitis. Levels of specific antibodies in the mucosa and serum were determined by enzyme-linked immunosorbent assay and immunogenic proteins of male and female parasites were identified using 2D electrophoresis and mass spectrometry. The function of identified proteins was explored with Blast2Go. Nematodes in mice with colitis induced higher levels of specific immunoglobulin G (IgG1) and IgA, a lower level of IgE in the small intestine and a higher level of IgE in serum against female L4. Infected mice with colitis recognized 12 proteins in male L4 and 10 in female L4. Most of the recognized proteins from male L4 were intermediate filament proteins, whereas the proteins from female L4 were primarily actins and galectins. Nematodes from mice with colitis were immunogenically different from nematodes from control mice. This phenomenon gives new insights into helminth therapy as well as host-parasite interactions.

journal_name

Parasitology

journal_title

Parasitology

authors

Maruszewska-Cheruiyot M,Donskow-Łysoniewska K,Krawczak K,Szewczak L,Joachimiak E,Doligalska M

doi

10.1017/S0031182020001201

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

1480-1487

issue

13

eissn

0031-1820

issn

1469-8161

pii

S0031182020001201

journal_volume

147

pub_type

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