Characterization of an atypical antigen from Sarcoptes scabiei containing an MADF domain.

Abstract:

:We have cloned a cDNA encoding a novel antigen from a Sarcoptes scabiei (Acari) cDNA library by immunoscreening with sera from S. scabiei-infected dogs. The antigen is encoded by a 2,157 bp mRNA with a predicted open reading frame of 719 amino acids (molecular weight 79 kDa). Our sequence analysis identified the presence of a MADF domain in the N-terminus, and downstream of this domain there was a region of low sequence complexity. This latter region contained several blocks of triplets and quadruplets of polar amino acids (Asn, Gln and Ser), and these 3 amino acids represented 39.7% of all amino acids. The antigen was named Atypical Sarcoptes Antigen 1 (ASA1) since the MADF domain normally is found in proteins involved in transcriptional regulation. In addition, 15 out of 62 S. scabiei-infected dogs reacted with a purified recombinant version of ASA1 in Western blot analysis. With immunohistochemistry we could show that ASA1 is expressed throughout the parasite, and that IgG specific for ASA1 binds to the inside wall of the mite's burrow. To our knowledge, this is the first description of an antigen containing an MADF domain.

journal_name

Parasitology

journal_title

Parasitology

authors

Ljunggren EL,Bergström K,Morrison DA,Mattsson JG

doi

10.1017/S0031182005008747

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

117-26

issue

Pt 1

eissn

0031-1820

issn

1469-8161

pii

S0031182005008747

journal_volume

132

pub_type

杂志文章
  • Plagiorchis elegans: emergence, longevity and infectivity of cercariae, and host behavioural modifications during cercarial emergence.

    abstract::We investigated changes in the behaviour of Stagnicola elodes associated with the emergence of Plagiorchis elegans cercariae. Within 15 min of the reduction in light intensity, which triggered the onset of cercarial emergence, infected snails moved to the top of the water column and remained there for 2-3 h. Seventy-n...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000077775

    authors: Lowenberger CA,Rau ME

    更新日期:1994-07-01 00:00:00

  • Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences.

    abstract::Phylogenetic relationships among Trypanosoma rangeli isolates from man, wild mammals and triatomine bugs from widespread geographical origin were inferred by comparison of the small subunit of ribosomal gene sequences. The phylogenetic trees indicated that the subgenus Herpetosoma is polyphyletic and strongly supporte...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004005931

    authors: Da Silva FM,Noyes H,Campaner M,Junqueira AC,Coura JR,Añez N,Shaw JJ,Stevens JR,Teixeira MM

    更新日期:2004-11-01 00:00:00

  • Parasites in space and time: a novel method to assess and illustrate host-searching behaviour of trematode cercariae.

    abstract::The transmission from one host to another constitutes a challenging obstacle for parasites and is a key determinant of their fitness. Due to their complex life histories involving several different hosts, the free-living dispersal stages (cercariae) of digenean trematodes show a huge diversity in morphology and behavi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182018000288

    authors: Selbach C,Poulin R

    更新日期:2018-09-01 00:00:00

  • Cystic echinococcosis: analysis of the serological profile related to the risk factors in individuals without ultrasound liver changes living in an endemic area of Tacuarembó, Uruguay.

    abstract::Cystic echinococcosis (CE) is a high prevalence zoonosis among the rural population of Tacuarembó (Uruguay). The correlation between serological data and the incidence of risk factors was studied in a survey carried out in 1998 among rural communities where 480 individuals were examined by means of abdominal sonograph...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004006717

    authors: Hernández A,Cardozo G,Dematteis S,Baz A,Trias N,Nuñez H,Barragué A,López L,Fuentes J,López O,Ferreira C

    更新日期:2005-04-01 00:00:00

  • Babesia parasites develop and are transmitted by the non-vector soft tick Ornithodoros moubata (Acari: Argasidae).

    abstract::Ornithodoros moubata ticks were fed on blood infected with Babesia equi. However, the parasites were quickly cleared as evidenced by the disappearance of B. equi-specific ribosomal RNA from the ticks. We hypothesized that if the Babesia parasite can escape midgut-associated barriers a non-vector tick can become infect...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006000916

    authors: Battsetseg B,Matsuo T,Xuan X,Boldbaatar D,Chee SH,Umemiya R,Sakaguchi T,Hatta T,Zhou J,Verdida AR,Taylor D,Fujisaki K

    更新日期:2007-01-01 00:00:00

  • Origin of a major infectious disease in vertebrates: The timing of Cryptosporidium evolution and its hosts.

    abstract::Protozoan parasites of the genus Cryptosporidium infect all vertebrate groups and display some host specificity in their infections. It is therefore possible to assume that Cryptosporidium parasites evolved intimately aside with vertebrate lineages. Here we propose a scenario of Cryptosporidium-Vertebrata coevolution ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001323

    authors: Garcia-R JC,Hayman DT

    更新日期:2016-11-01 00:00:00

  • Decay of similarity with host phylogenetic distance in parasite faunas.

    abstract::Exponential decay in community similarity as a function of distance is a ubiquitous phenomenon in biogeography. Thus, for parasite communities, pairwise similarity decreases with increasing geographical distance between host populations. This biogeographical rule should also apply along other dimensions characterizing...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009991491

    authors: Poulin R

    更新日期:2010-04-01 00:00:00

  • Hymenolepis nana: the fine structure of the embryonic envelopes.

    abstract::The fine structure of the envelopes surrounding hatched and unhatched oncospheres of Hymenolepis nana has been investigated by transmission and scanning electron microscopy (SEM), together with light microscope histochemical observations of JB-4 embedded material. The oncosphere is surrounded by 3 layers--the capsule,...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000066968

    authors: Fairweather I,Threadgold LT

    更新日期:1981-06-01 00:00:00

  • Insights on adaptive and innate immunity in canine leishmaniosis.

    abstract::Canine leishmaniosis (CanL) is caused by the parasite Leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. Clinical manifestations can range from subclinical infection to very severe systemic disease. Leishmaniosis is categorized as a neg...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201600055X

    authors: Hosein S,Blake DP,Solano-Gallego L

    更新日期:2017-01-01 00:00:00

  • Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism.

    abstract::Isolates of Trypanosoma cruzi from human patients, domestic and sylvatic animals and vector insects were obtained in different areas of Argentina. Electrophoretic patterns of enzymes from extracts of 95 isolates were analysed. On the basis of zymograms providing information on 10 loci, 12 zymodemes are described accor...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000067755

    authors: de Luca D'oro GM,Gardenal CN,Perret B,Crisci JV,Montamat EE

    更新日期:1993-11-01 00:00:00

  • Relative host body condition and food availability influence epidemic dynamics: a Poecilia reticulata-Gyrodactylus turnbulli host-parasite model.

    abstract::Understanding disease transmission is important to species management and human health. Host body condition, nutrition and disease susceptibility interact in a complex manner, and while the individual effects of these variables are well known, our understanding of how they interact and translate to population dynamics...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182012001667

    authors: Tadiri CP,Dargent F,Scott ME

    更新日期:2013-03-01 00:00:00

  • Characterization of the mitochondrial genome of Diphyllobothrium latum (Cestoda: Pseudophyllidea) - implications for the phylogeny of eucestodes.

    abstract::The complete nucleotide sequence of the mitochondrial genome was determined for the fish tapeworm Diphyllobothrium latum. This genome is 13,608 bp in length and encodes 12 protein-coding genes (but lacks the atp8), 22 transfer RNA (tRNA) and 2 ribosomal RNA (rRNA) genes, corresponding to the gene complement found thus...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118200600206X

    authors: Park JK,Kim KH,Kang S,Jeon HK,Kim JH,Littlewood DT,Eom KS

    更新日期:2007-05-01 00:00:00

  • The degradation rates of cytoplasmic tRNA, rRNA and mRNA in rats are elevated after infection with Nippostrongylus brasiliensis.

    abstract::The effects of a parasitic infection with the nematode Nippostrongylus brasiliensis on the degradation rates of cytoplasmic tRNA, rRNA and mRNA in rats have been investigated by measuring the renal excretion rates of the modified RNA catabolites N6-threoninocarbonyladenosine, pseudouridine and 7-methylguanine. Between...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182097002102

    authors: Topp H,Duden R,Stephan U,Schöch G

    更新日期:1998-02-01 00:00:00

  • Down-regulation of murine susceptibility to cerebral malaria by inoculation with third-stage larvae of the filarial nematode Brugia pahangi.

    abstract::In areas where malaria is endemic, helminthic infections, caused by intestinal or filarial parasites, commonly coexist with malaria in the same individual. This study investigates the course of Plasmodium berghei malaria infection in CBA/J mice inoculated with irradiated attenuated 3rd-stage larvae (L3) of Brugia paha...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182096008566

    authors: Yan Y,Inuo G,Akao N,Tsukidate S,Fujita K

    更新日期:1997-04-01 00:00:00

  • Detection of persistent Plasmodium spp. infections in Ugandan children after artemether-lumefantrine treatment.

    abstract::During a longitudinal study investigating the dynamics of malaria in Ugandan lakeshore communities, a consistently high malaria prevalence was observed in young children despite regular treatment. To explore the short-term performance of artemether-lumefantrine (AL), a pilot investigation into parasite carriage after ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201400033X

    authors: Betson M,Sousa-Figueiredo JC,Atuhaire A,Arinaitwe M,Adriko M,Mwesigwa G,Nabonge J,Kabatereine NB,Sutherland CJ,Stothard JR

    更新日期:2014-12-01 00:00:00

  • Host-parasite relationship between congenital Toxoplasma infection and mouse brain: role of small vessels.

    abstract::Small vessels showing inflammatory cell infiltrates were invariably observed in the vicinity of intact Toxoplasma tissue cysts within the brains of mice congenitally infected with the protozoan. Lymphocytes were observed in intimate contact with the luminal aspect of the endothelium, penetrating into the thickened bas...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063873

    authors: Sims TA,Hay J

    更新日期:1995-02-01 00:00:00

  • First report of Trypanosoma dionisii (Trypanosomatidae) identified in Australia.

    abstract::Trypanosomes are blood-borne parasites that can infect a variety of different vertebrates, including animals and humans. This study aims to broaden scientific knowledge about the presence and biodiversity of trypanosomes in Australian bats. Molecular and morphological analysis was performed on 86 blood samples collect...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020001845

    authors: Austen JM,Van Kampen E,Egan SL,O'Dea MA,Jackson B,Ryan UM,Irwin PJ,Prada D

    更新日期:2020-12-01 00:00:00

  • Biological approaches to characterize the mode of action of two 5-nitroindazolinone prototypes on Trypanosoma cruzi bloodstream trypomastigotes.

    abstract::The phenotypic activity of two 5-nitroindazolinones, i.e. 2-benzyl-1-propyl (22) and 2-benzyl-1-butyl (24) derivatives, previously proposed as anti-Trypanosoma cruzi prototypes, was presently assayed on bloodstream trypomastigotes (BT) of the moderately drug-resistant Y strain. Further exploration of putative targets ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001098

    authors: Fonseca-Berzal C,DA Silva CF,Menna-Barreto RF,Batista MM,Escario JA,Arán VJ,Gómez-Barrio A,Soeiro Mde N

    更新日期:2016-09-01 00:00:00

  • Multi-scale spatial analysis of human alveolar echinococcosis risk in China.

    abstract::Risk factors for the transmission of Echinococcus multilocularis to humans operate at a range of spatial scales. Over a large area, such as China, regional scale risk is correlated with variation in climatic conditions because of its effect on the spatial distribution of landscapes that can support E. multilocularis t...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:

    authors: Danson FM,Graham AJ,Pleydell DR,Campos-Ponce M,Giraudoux P,Craig PS

    更新日期:2003-01-01 00:00:00

  • Parasites as biological tags of fish stocks: a meta-analysis of their discriminatory power.

    abstract::The use of parasites as biological tags to discriminate among marine fish stocks has become a widely accepted method in fisheries management. Here, we first link this approach to its unstated ecological foundation, the decay in the similarity of the species composition of assemblages as a function of increasing distan...

    journal_title:Parasitology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1017/S0031182013001534

    authors: Poulin R,Kamiya T

    更新日期:2015-01-01 00:00:00

  • Repeated infection of cats with Brugia pahangi: parasitological observations.

    abstract::Cats were repeatedly inoculated with infective larvae of Brugia pahangi. On parasitological grounds they could be divided into 5 groups. Group I--most cats (some 70%) became microfilaraemic (mf+) and retained high levels of microfilariae (mf) in their blood for over 2 years. In some Group I cats mf counts stabilized a...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063666

    authors: Denham DA,Medeiros F,Baldwin C,Kumar H,Midwinter IC,Birch DW,Smail A

    更新日期:1992-06-01 00:00:00

  • Capture and penetration processes of the free-living juveniles of Trichostrongylus colubriformis (Nematoda) by the nematophagous fungus, Arthrobotrys oligospora.

    abstract::The nematode-trapping fungus Arthrobotrys oligospora traps and invades all the free-living juvenile stages of the trichostrongyle nematode Trichostrongylus colubriformis. The processes of capture and invasion of the 1st-stage juveniles are described using differential interference contrast optics and 3-D reconstructio...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000079798

    authors: Murray DS,Wharton DA

    更新日期:1990-08-01 00:00:00

  • Observations on the surface morphology of the ovarian balls of Moniliformis (Acanthocephala).

    abstract::The surface morphology of fixed ovarian balls from female Moniliformis dubius, agd 12, 18, 26, 28, 42, 77, 105 and 118 days, has been examined by means of scanning electron microscopy. The appearance of the surface has been found to be variable between ovarian balls from worms of the same and different ages. Some part...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000056146

    authors: Parshad VR,Crompton DW,Martin J

    更新日期:1980-10-01 00:00:00

  • Cryptostatin, a chagasin-family cysteine protease inhibitor of Cryptosporidium parvum.

    abstract::Cysteine proteases of pathogenic protozoan parasites play pivotal roles in the life cycle of parasites, but strict regulation of their activities is also essential for maintenance of parasite physiology and interaction with hosts. In this study, we identified and characterized cryptostatin, a novel inhibitor of cystei...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182012000297

    authors: Kang JM,Ju HL,Yu JR,Sohn WM,Na BK

    更新日期:2012-07-01 00:00:00

  • Trypanosoma congolense: appearance and distribution of variable antigen types during metacyclic differentiation in vitro.

    abstract::Differentiation of epimastigotes and production of infective metacyclic forms of Trypanosoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:

    authors: Prain CJ,Ross CA

    更新日期:1990-02-01 00:00:00

  • Neospora caninum cytoplasmic dynein LC8 light chain 2 (NcDYNLL2) is differentially produced by pathogenically distinct isolates and regulates the host immune response.

    abstract::Neospora caninum is the causative agent of bovine neosporosis. A N. caninum cytoplasmic dynein LC8 light chain (NcDYNLL) protein was characterized in this study. Cytoplasmic dyneins, including DYNLLs, belong to the microtubule minus-end-directed motor proteins and are involved in many cellular processes. Previous micr...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201800207X

    authors: Cao L,Fetterer R,Qu G,Zhang X,Tuo W

    更新日期:2018-12-18 00:00:00

  • Immunogenicity of a hybrid Plasmodium falciparum malaria antigen.

    abstract::A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000076733

    authors: Lockyer MJ,Cooper H,Tite J,Rowan W,Crowe JS

    更新日期:1993-06-01 00:00:00

  • The effects of age, temperature, light quantity and wavelength on the swimming behaviour of the cercariae of Cryptocotyle lingua (Digenea: Heterophyidae).

    abstract::Laboratory experiments indicated that the active life-span of the cercariae of Cryptocotyle lingua was temperature-dependent. An increase in water temperature and population age both correlated with a tendency for the organisms to become decaudate. The larvae were strongly photoresponsive to lateral light but with tim...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000073789

    authors: Rea JG,Irwin SW

    更新日期:1992-08-01 00:00:00

  • Ribonucleic acid synthesis in Plasmodium knowlesi maintained both in vivo and in vitro.

    abstract::RNA extracted from purified parasites of Plasmodium knowlesi was fractionated using agarose gel electrophoresis. Preparations from para sites grown both in vivo and in vitro contained species of RNA with sedimentation coefficients of 4-0S, 5-0S, 16-6S, 24-2S, 31-4S, 38-0S and 48-3S. There was less RNA present in paras...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000046655

    authors: Trigg PI,Shakespeare PG,Burt SJ,Kyd SI

    更新日期:1975-10-01 00:00:00

  • Characterization of surface proteins and glycoproteins on red blood cells from mice infected with haemosporidia: Plasmodium berghei infections of BALB/c mice.

    abstract::The surface proteins and glycoproteins of red cells from Plasmodium berghei-infected blood have been radio-isotope labelled and compared with those of normal mouse erythrocytes using the following protein labelling probes: lactoperoxidase-catalysed radio-iodination of tyrosyl residues, periodate oxidation and NaB3H4 r...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000056031

    authors: Howard RJ,Smith PM,Mitchell GF

    更新日期:1980-10-01 00:00:00