A panel of antigens of muscle larvae of Trichinella spiralis and T. pseudospiralis as revealed by two-dimensional western blot and immunoelectron microscopy.

Abstract:

:This study characterized antigens of Trichinella spiralis and T. pseudospiralis muscle larvae recognized by mice infected with the worms. Two-dimensional (2-D) Western blot analysis revealed some profile of antigenic peptides including: (1) molecular weight (MW); (2) isoelectric points (pI), (3) reactivity to well-defined monoclonal antibodies (mAb) and (4) cross-reactivity between the 2 species. Antigenic peptides of T. spiralis consisted of about 100 spots. The MW ranged from 22 to 80 kDa, and pI ranged from 4 to 7. The mAb against TSL-1 stained most of the T. spiralis excretory-secretory (E-S) peptides migrating at 40, 45 and 50 kDa, and the mAb against TSL-4 stained non-E-S peptides. Antigenic peptides of T. pseudospiralis consisted of about 20 to 30 peptide spots. The MW ranged from 25 to 80 kDa, and pI ranged from 4 to 7. The mAb against TSL-1 stained most of the T. pseudospiralis E-S peptides migrating at 35 and 45 kDa, and the mAb against TSL-4 stained non-E-S peptides. Two-dimensional Western blots showed that the E-S products of T. spiralis and T. pseudospiralis were highly cross-reactive with each other. The non-E-S peptides were, however, not recognized by T. pseudospiralis-infected sera but were recognized by T. spiralis-infected sera. An immunoelectron microscopical study showed the similar result that stichocyte granules and cuticle surface (known to contain E-S antigen) had cross-reactive antigens between the two species. T. pseudospiralis-infected sera stained very weakly the cuticle inner layers and haemolymph (known to contain non-E-S antigen). This evidence implies that mice infected with T. pseudospiralis do not evoke antibodies against non-E-S antigen at the detectable level.

journal_name

Parasitology

journal_title

Parasitology

authors

Wu Z,Nagano I,Takahashi Y

doi

10.1017/s0031182099004187

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

615-22

eissn

0031-1820

issn

1469-8161

journal_volume

118 ( Pt 6)

pub_type

杂志文章
  • Improved serodiagnosis of alveolar echinococcosis of humans using an in vitro-produced Echinococcus multilocularis antigen.

    abstract::Serology is an important tool for the diagnosis of alveolar echinococcosis (AE) in humans. In order to improve serodiagnostic performance, we have developed an in vitro-produced Echinococcus mulilocularis metacestode vesicle fluid (EmVF) antigen for application in an immunoblot assay. Immunoblot analysis of EmVF revea...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006002083

    authors: Müller N,Frei E,Nuñez S,Gottstein B

    更新日期:2007-06-01 00:00:00

  • Biological approaches to characterize the mode of action of two 5-nitroindazolinone prototypes on Trypanosoma cruzi bloodstream trypomastigotes.

    abstract::The phenotypic activity of two 5-nitroindazolinones, i.e. 2-benzyl-1-propyl (22) and 2-benzyl-1-butyl (24) derivatives, previously proposed as anti-Trypanosoma cruzi prototypes, was presently assayed on bloodstream trypomastigotes (BT) of the moderately drug-resistant Y strain. Further exploration of putative targets ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001098

    authors: Fonseca-Berzal C,DA Silva CF,Menna-Barreto RF,Batista MM,Escario JA,Arán VJ,Gómez-Barrio A,Soeiro Mde N

    更新日期:2016-09-01 00:00:00

  • Detection of Neospora caninum in aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin, China.

    abstract::Neospora caninum infection is a significant cause of abortion in cattle. We investigated the tissue distribution of N. caninum in aborted bovine fetuses and dam blood samples by a nested PCR assay, and compared the nested PCR with ELISA in the diagnosis of N. caninum infection. In total, 26 aborted fetuses and 813 blo...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009990813

    authors: Yao L,Yang N,Liu Q,Wang M,Zhang W,Qian WF,Hu YF,Ding J

    更新日期:2009-09-01 00:00:00

  • Resistance towards monensin is proposed to be acquired in a Toxoplasma gondii model by reduced invasion and egress activities, in addition to increased intracellular replication.

    abstract::Monensin (Mon) is an anticoccidial polyether ionophore widely used to control coccidiosis. The extensive use of polyether ionophores on poultry farms resulted in widespread resistance, but the underlying resistance mechanisms are unknown in detail. For analysing the mode of action by which resistance against polyether...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017001512

    authors: Thabet A,Schmidt J,Baumann S,Honscha W,VON Bergen M,Daugschies A,Bangoura B

    更新日期:2018-03-01 00:00:00

  • Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia malayi from Northeast Borneo and Thailand.

    abstract::Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed space...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182012001242

    authors: Fong MY,Noordin R,Lau YL,Cheong FW,Yunus MH,Idris ZM

    更新日期:2013-01-01 00:00:00

  • Comparison of the efficacy of tea tree (Melaleuca alternifolia) oil with other current pharmacological management in human demodicosis: A Systematic Review.

    abstract::Various treatments are found to be moderately effective in managing Demodex-related diseases except tea tree oil (TTO) and terpinen-4-ol (T4O), which showed superior miticidal and anti-inflammatory effects in numerous clinical studies. Their possible effects include lowering mite counts, relieving Demodex-related symp...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118202000150X

    authors: Lam NSK,Long XX,Li X,Yang L,Griffin RC,Doery JC

    更新日期:2020-12-01 00:00:00

  • The effects of age, temperature, light quantity and wavelength on the swimming behaviour of the cercariae of Cryptocotyle lingua (Digenea: Heterophyidae).

    abstract::Laboratory experiments indicated that the active life-span of the cercariae of Cryptocotyle lingua was temperature-dependent. An increase in water temperature and population age both correlated with a tendency for the organisms to become decaudate. The larvae were strongly photoresponsive to lateral light but with tim...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000073789

    authors: Rea JG,Irwin SW

    更新日期:1992-08-01 00:00:00

  • Rodent malaria parasites suffer from the presence of conspecific clones in three-clone Plasmodium chabaudi infections.

    abstract::We studied infection dynamics of Plasmodium chabaudi in mice infected with 3 genetically distinct clones--1 less virulent than the other 2--either on their own or in mixtures. During the acute phase of infection, total numbers of asexual parasites in mixed-clone infections were equal to those produced by the 3 clones ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182003004001

    authors: De Roode JC,Read AF,Chan BH,Mackinnon MJ

    更新日期:2003-11-01 00:00:00

  • The ultrastructure and immunogold labelling of pancreatic polypeptide-immunoreactive cells associated with the egg-forming apparatus of a monogenean parasite, Diclidophora merlangi.

    abstract::An electron microscopical examination has been made of the fine structure and disposition of pancreatic polypeptide immunoreactive cells associated with the egg-forming apparatus in Diclidophora merlangi. The cell bodies are positioned in the parenchyma surrounding the ootype and taper to axon-like processes that exte...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000064404

    authors: Halton DW,Brennan GP,Maule AG,Shaw C,Johnston CF,Fairweather I

    更新日期:1991-06-01 00:00:00

  • Apparent tolerance of Plasmodium falciparum in infants in a highly endemic area.

    abstract::The incidence of fever among infants in the village of Idete in the Morogoro region of Tanzania was analyzed in relation to densities of Plasmodium falciparum parasites in the peripheral blood. Parasite densities in both fever cases and in asymptomatic infants, were compared and a Bayesian non-parametric mixture decom...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005211

    authors: Vounatsou P,Smith T,Kitua AY,Alonso PL,Tanner M

    更新日期:2000-01-01 00:00:00

  • Eimeria species which infect the chicken contain virus-like RNA molecules.

    abstract::There is increasing support for the presence of viruses and virus-like particles inside protozoan cells. This study describes viral-like RNA molecules that have been detected in two species of Eimeria that infect the chicken. The RNA molecule identified in E. maxima has been characterized: subcellular fractionation st...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063198

    authors: Ellis J,Revets H

    更新日期:1990-10-01 00:00:00

  • Tegumental disruption with Triton X-100 and its effects on longitudinal muscle function in male Schistosoma mansoni.

    abstract::Longitudinal muscles in adult male Schistosoma mansoni appear to remain in almost all respects functional after tegumental disruption by Triton X-100. Responses to high K+, ouabain, low temperature, praziquantel and neurotransmitters are still present. Muscle membrane potentials remain near those of control animals an...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000052422

    authors: Depenbusch JW,Thompson DP,Pax RA,Bennett JL

    更新日期:1983-08-01 00:00:00

  • Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism.

    abstract::Isolates of Trypanosoma cruzi from human patients, domestic and sylvatic animals and vector insects were obtained in different areas of Argentina. Electrophoretic patterns of enzymes from extracts of 95 isolates were analysed. On the basis of zymograms providing information on 10 loci, 12 zymodemes are described accor...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000067755

    authors: de Luca D'oro GM,Gardenal CN,Perret B,Crisci JV,Montamat EE

    更新日期:1993-11-01 00:00:00

  • Identification of a developmentally regulated Schistosoma mansoni serine protease homologous to mouse plasma kallikrein and human factor I.

    abstract::The isolation of 2 genomic clones has allowed us to further characterize a Schistosoma mansoni serine protease designated SmSP1. The deduced amino acid sequence (248aa) considered as a 'light chain' encoding the active site, presents significant homologies with mouse plasma kallikrein and human factor I light chain. T...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182098003874

    authors: Cocude C,Pierrot C,Cêtre C,Fontaine J,Godin C,Capron A,Khalife J

    更新日期:1999-04-01 00:00:00

  • Hatching in the monogenean parasite Dictyocotyle coeliaca from the body cavity of Raja naevus.

    abstract::When eggs of the monogenean Dictyocotyle coeliaca, a parasite from the body cavity of Raja naevus, are incubated in alternating 12 h periods of light and darkness at 12 degrees C the marginal hooklets appear between 75 and 90 days after collecting the eggs from the host's body cavity and hatching begins after about 10...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000048897

    authors: Kearn GC

    更新日期:1975-02-01 00:00:00

  • Machine learning-based analyses support the existence of species complexes for Strongyloides fuelleborni and Strongyloides stercoralis.

    abstract::Human strongyloidiasis is a serious disease mostly attributable to Strongyloides stercoralis and to a lesser extent Strongyloides fuelleborni, a parasite mainly of non-human primates. The role of animals as reservoirs of human-infecting Strongyloides is ill-defined, and whether dogs are a source of human infection is ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020000979

    authors: Barratt JLN,Sapp SGH

    更新日期:2020-09-01 00:00:00

  • Expression of LPG and GP63 by different developmental stages of Leishmania major in the sandfly Phlebotomus papatasi.

    abstract::Development and forward migration of Leishmania parasites in the sandfly gut is accompanied by morphological transformation to highly motile, non-dividing 'metacyclic' forms. Previous studies in vitro have demonstrated that this metacyclogenesis is associated with developmentally regulated changes in expression of two...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000060522

    authors: Davies CR,Cooper AM,Peacock C,Lane RP,Blackwell JM

    更新日期:1990-12-01 00:00:00

  • Pentastomids and the tetrapod lung.

    abstract::Pentastomids comprise a highly specialized taxon of arthropod-like parasites that probably became adapted to the lungs of amphibians and reptiles early in their long evolutionary history. Few other macroparasites exploit this particular niche. Pentastomids are often large, long-lived and yet they cause little observab...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:

    authors: Riley J,Henderson RJ

    更新日期:1999-01-01 00:00:00

  • Host-parasite interactions and global climate oscillations.

    abstract::It is suspected that host-parasite interactions are influenced by climatic oscillations such as the North Atlantic Oscillation (NAO). However, the effects of climatic oscillations on host-parasite interactions have never been investigated. A long-term (1982-1999) dataset of the host snail Lymnaea stagnalis and tremato...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011000655

    authors: Doi H,Yurlova NI

    更新日期:2011-07-01 00:00:00

  • Alternative NADH dehydrogenase (NDH2): intermembrane-space-facing counterpart of mitochondrial complex I in the procyclic Trypanosoma brucei.

    abstract::The respiratory chain of the procyclic stage of Trypanosoma brucei contains the standard complexes I through IV, as well as several alternative enzymes contributing to electron flow. In this work, we studied the function of an alternative NADH : ubiquinone oxidoreductase (NDH2). Depletion of target mRNA was achieved u...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201200162X

    authors: Verner Z,Skodová I,Poláková S,Durišová-Benkovičová V,Horváth A,Lukeš J

    更新日期:2013-03-01 00:00:00

  • What helminth genomes have taught us about parasite evolution.

    abstract::The genomes of more than 20 helminths have now been sequenced. Here we perform a meta-analysis of all sequenced genomes of nematodes and Platyhelminthes, and attempt to address the question of what are the defining characteristics of helminth genomes. We find that parasitic worms lack systems for surface antigenic var...

    journal_title:Parasitology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1017/S0031182014001449

    authors: Zarowiecki M,Berriman M

    更新日期:2015-02-01 00:00:00

  • Dynamics of Sept4 expression in fibrotic livers of mice infected with Schistosoma japonicum.

    abstract::n order to investigate the dynamics of Septin4 (Sept4) expression and its function in the formation of fibrotic livers in mice infected with Schistosoma japonicum, we constructed the mouse model of S. japonicum egg-induced liver fibrosis for 24 weeks. Immunohistochemical staining, qRT-PCR and Western blot were used to...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011000667

    authors: Duan YN,Qian HY,Qin YW,Zhu DD,He XX,Zhou Q,Yang YN,Bao J,Feng JR,Sun W,Chen JL

    更新日期:2011-07-01 00:00:00

  • Parasites as biological tags of fish stocks: a meta-analysis of their discriminatory power.

    abstract::The use of parasites as biological tags to discriminate among marine fish stocks has become a widely accepted method in fisheries management. Here, we first link this approach to its unstated ecological foundation, the decay in the similarity of the species composition of assemblages as a function of increasing distan...

    journal_title:Parasitology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1017/S0031182013001534

    authors: Poulin R,Kamiya T

    更新日期:2015-01-01 00:00:00

  • Subtype analysis of Cryptosporidium parvum isolates from calves on farms around Belgrade, Serbia and Montenegro, using the 60 kDa glycoprotein gene sequences.

    abstract::Cryptosporidium parvum is composed of multiple subtypes, which appear to be zoonotic or anthroponotic. Therefore, in order to understand the molecular epidemiology of this species and to control the zoonotic transmission, it is essential to identify the isolates at the subtype level. In the present study, 60.2% of 103...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006001508

    authors: Misic Z,Abe N

    更新日期:2007-03-01 00:00:00

  • Echinococcus granulosus: intraspecific genetic variation assessed by a DNA repetitive element.

    abstract::A 186 bp Echinococcus granulosus-specific repetitive element, TREg, was used to assess genetic variation between strains. In G7 genotype (pig strain) it has the characteristics of a satellite DNA element with a copy number of 23000 per haploid genome. Analysis, by sequencing of TREg monomers, showed a great degree of ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001008575

    authors: Rosenzvit MC,Canova SG,Kamenetzky L,Guarnera EA

    更新日期:2001-10-01 00:00:00

  • Blood schizontocidal activity of azithromycin and its combination with alpha/beta arteether against multi-drug resistant Plasmodium yoelii nigeriensis, a novel MDR parasite model for antimalarial screening.

    abstract::Many different drug-resistant lines of rodent malaria are available as screening models. It is obligatory to screen new compounds for antimalarial activity against a series of resistant lines in order to identify a compound with potential for the treatment of multi-drug resistant (MDR) malaria infections. Instead of u...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118200500778x

    authors: Tripathi R,Dhawan S,Dutta GP

    更新日期:2005-09-01 00:00:00

  • Are we ready for Taenia solium cysticercosis elimination in sub-Saharan Africa?

    abstract::The World Health Organization announced in November 2014 at the fourth international meeting on 'the control of neglected zoonotic diseases - from advocacy to action', that intervention tools for eliminating Taenia solium taeniosis/cysticercosis (TSTC) are in place. The aim of this work was to elucidate theoretical ou...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016000500

    authors: Johansen MV,Trevisan C,Gabriël S,Magnussen P,Braae UC

    更新日期:2017-01-01 00:00:00

  • Taenia crassiceps: in vivo and in vitro models.

    abstract::Taenia crassiceps is a cestode parasite of wild and domestic animals that rarely affects humans; it has been widely used as an experimental model. The asexual proliferation by budding is a useful attribute of T. crassiceps cysticerci, which allows the various strains to be maintained indefinitely in the peritoneal cav...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182009991442

    authors: Willms K,Zurabian R

    更新日期:2010-03-01 00:00:00

  • Antigenic variation during Trypanosoma vivax infections of different host species.

    abstract::The sequence of appearance of specific lytic activity against more than 20 variable antigen types (VATs) of Trypanosoma vivax in the serum of 27 animals belonging to 5 species has been examined. For each host species there was a characteristic course of infection, with differences in height and duration of parasitaemi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063447

    authors: Barry JD

    更新日期:1986-02-01 00:00:00

  • Vector survival and parasite infection: the effect of Wuchereria bancrofti on its vector Culex quinquefasciatus.

    abstract::This paper investigates a cohort of 2187 laboratory reared Culex quinquefasciatus fed on 69 human volunteers, including 59 persons with different levels of Wuchereria bancrofti microfilariae and 10 without microfilaria. Mosquitoes were followed until death. Mosquito survival was analysed in relation to the level of mi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004005153

    authors: Krishnamoorthy K,Subramanian S,Van Oortmarssen GJ,Habbema JD,Das PK

    更新日期:2004-07-01 00:00:00