Abstract:
:A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of the antibody response to the CSP repeats was not overcome by the PMMSA component, the response to which showed no restriction. In an adjuvant trial the highest antibody titres in rabbits to both components of the hybrid were obtained using Freund's adjuvant. Lack of a boosting antibody response to the CSP repeats appeared to be linked to the conformation of the PMMSA component. Formulation of the hybrid protein into Iscoms gave antibody titres of only short duration to both components.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Lockyer MJ,Cooper H,Tite J,Rowan W,Crowe JSdoi
10.1017/s0031182000076733subject
Has Abstractpub_date
1993-06-01 00:00:00pages
451-7eissn
0031-1820issn
1469-8161journal_volume
106 ( Pt 5)pub_type
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