Abstract:
:Cystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Velásquez JN,Osvaldo GA,Di Risio C,Etchart C,Chertcoff AV,Perissé GE,Carnevale Sdoi
10.1017/S0031182010001253subject
Has Abstractpub_date
2011-03-01 00:00:00pages
279-86issue
3eissn
0031-1820issn
1469-8161pii
S0031182010001253journal_volume
138pub_type
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