Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome.

Abstract:

:Cystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli.

journal_name

Parasitology

journal_title

Parasitology

authors

Velásquez JN,Osvaldo GA,Di Risio C,Etchart C,Chertcoff AV,Perissé GE,Carnevale S

doi

10.1017/S0031182010001253

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

279-86

issue

3

eissn

0031-1820

issn

1469-8161

pii

S0031182010001253

journal_volume

138

pub_type

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