New defined and semi-defined media for cultivation of the flagellate Trichomonas vaginalis.

Abstract:

:Studies on the nutrition and physiology of Trichomonas vaginalis have been hampered by the lack of a defined synthetic medium in which to grow the organism. New synthetic media free of ill-defined supplements and serum which support good growth of T. vaginalis have been developed in this study. The first successful approach to a defined medium resulted from the modification of the tissue-culture medium CMRL 1066. Essential modifications included (1) lowering the redox potential of the medium by adding high levels of ascorbate and (2) modifying the buffer system. Subsequently, CMRL 1066 was replaced by two new media DL7 and DL8 which have greater flexibility in their formulation and permit improved serum-free growth. Serum was replaced by bovine serum albumin and cholesterol, together with either a glyceryl fatty acid ester or a defined mixture of fatty acids. Preliminary nutritional studies using the new media have indicated a requirement for saturated and unsaturated fatty acids and a sterol.

journal_name

Parasitology

journal_title

Parasitology

authors

Linstead D

doi

10.1017/s0031182000050101

subject

Has Abstract

pub_date

1981-08-01 00:00:00

pages

125-37

issue

Pt 1

eissn

0031-1820

issn

1469-8161

journal_volume

83

pub_type

杂志文章
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  • Differentiation of Schistosoma mansoni from S. rodhaini using cloned DNA probes.

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  • Female mice mate preferentially with non-parasitized males.

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  • Mitochondrial DNA and Ascaris microepidemiology: the composition of parasite populations from individual hosts, families and villages.

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  • The virus infecting the parasitoid Leptopilina boulardi exerts a specific action on superparasitism behaviour.

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  • The effect of Giardia lamblia trophozoites on trypsin, chymotrypsin and amylase in vitro.

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  • Cytochemical studies on nuclear DNA of four eucoccidian parasites, Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei.

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    journal_title:Parasitology

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    authors: Cornelissen AW,Overdulve JP,Van der Ploeg M

    更新日期:1984-02-01 00:00:00

  • Relocation of Schistosoma mansoni in the lungs and resistance to reinfection in Rattus rattus.

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  • Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism.

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  • The emergence of macrocyclic lactone resistance in the canine heartworm, Dirofilaria immitis.

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  • Effect of acute phase proteins, especially alpha 2-macroglobulin, on granuloma formation around Schistosoma mansoni eggs in the rat.

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  • Parasite-host relationships during the development of Eimeria dispersa Tyzzer 1929, in the turkey (Meleagris gallopavo gallopavo) with a description of intestinal intra-epithelial leucocytes.

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  • Dexamethasone downregulates the expressions of MMP-9 and oxidative stress in mice with eosinophilic meningitis caused by Angiostrongylus cantonensis infection.

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    abstract::Seroprevalence data illustrate that human exposure to Toxocara is frequent. Environmental contamination with Toxocara spp. eggs is assumed to be the best indicator of human exposure, but increased risk of exposure has also been associated with many other factors. Reported associations are inconsistent, however, and th...

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