Differentiation of Schistosoma mansoni from S. rodhaini using cloned DNA probes.

Abstract:

:The ribosomal RNA (rRNA) gene units of Schistosoma mansoni and S. rodhaini, of the lateral spined egg group, have been studied. The schistosome rRNA gene unit consists of a regular interspersion of the two genes encoding the large and small rRNA units with two spacers. The large spacer is not transcribed while the small spacer is part of the transcription unit. Variation in the rRNA gene unit between the two species is demonstrated to take the form of loss or gain of restriction sites within the non-transcribed and transcribed spacers. This variation has been demonstrated to enable the differentiation of S. mansoni from S. rodhaini by Southern hybridization analysis. In addition, a DNA clone representing a female specific, tandemly repeated sequence, is demonstrated to enable differentiation of S. mansoni and S. rodhaini using dot blot hybridization analysis.

journal_name

Parasitology

journal_title

Parasitology

authors

Walker TK,Simpson AJ,Rollinson D

doi

10.1017/s0031182000059709

subject

Has Abstract

pub_date

1989-02-01 00:00:00

pages

75-80

eissn

0031-1820

issn

1469-8161

journal_volume

98 ( Pt 1)

pub_type

杂志文章
  • Field evidence for density-dependent facilitation amongst Ixodes ricinus ticks feeding on sheep.

    abstract::Engorged Ixodes ricinus nymphs collected from sheep resident in an upland UK field site were significantly lighter than nymphs that engorged on previously tick-naïve sheep, indicating that site-resident sheep continually exposed to ticks acquired anti-tick resistance. The weights of engorged nymphs that fed on natural...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001001081

    authors: Ogden NH,Casey AN,French NP,Adams JD,Woldehiwet Z

    更新日期:2002-02-01 00:00:00

  • Kinetic modelling of isometamidium chloride (Samorin) uptake by Trypanosoma congolense.

    abstract::Clones of Trypanosoma congolense which express resistance to the widely used trypanocide isometamidium chloride accumulate less of the drug than clones which are sensitive to drug treatment. A mathematical model has been developed which was able to predict theoretical lines representing the uptake kinetics in trypanos...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000073728

    authors: Sutherland IA,Mounsey A,Eisler M,Holmes PH

    更新日期:1992-08-01 00:00:00

  • Decay of similarity with host phylogenetic distance in parasite faunas.

    abstract::Exponential decay in community similarity as a function of distance is a ubiquitous phenomenon in biogeography. Thus, for parasite communities, pairwise similarity decreases with increasing geographical distance between host populations. This biogeographical rule should also apply along other dimensions characterizing...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009991491

    authors: Poulin R

    更新日期:2010-04-01 00:00:00

  • Peromyscus populations and their Cuterebra parasites display congruent phylogeographical structure.

    abstract::The relationships between populations of the Deer Mouse (Peromyscus maniculatus) and the White-footed Mouse (P. leucopus) and their respective Cuterebra parasites were examined. Population genetic structure of hosts and parasites was inferred using cytochrome oxidase mitochondrial sequences of specimens from 7 populat...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182005007584

    authors: Noel S,Angers B,Lapointe FJ

    更新日期:2005-08-01 00:00:00

  • Towards a comprehensive simulation model of malaria epidemiology and control.

    abstract::Planning of the control of Plasmodium falciparum malaria leads to a need for models of malaria epidemiology that provide realistic quantitative prediction of likely epidemiological outcomes of a wide range of control strategies. Predictions of the effects of control often ignore medium- and long-term dynamics. The com...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008000371

    authors: Smith T,Maire N,Ross A,Penny M,Chitnis N,Schapira A,Studer A,Genton B,Lengeler C,Tediosi F,de Savigny D,Tanner M

    更新日期:2008-11-01 00:00:00

  • Allosteric control of pyrimidine transport in Hymenolepis diminuta: an unusual kinetic isotope effect.

    abstract::A thymine-uracil transport system is present in the plasma membrane brush-border of the tapeworm, Hymenolepis diminuta. The relation between initial uptake and substrate concentration of either thymine or uracil was sigmoidal when 2-14C-labelled substrates were used. In contrast, absorption kinetics of methyl-14C- and...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000052641

    authors: Uglem GL,Dupre RK,Harley JP

    更新日期:1983-10-01 00:00:00

  • Mechanisms underlying reduced expulsion of a murine nematode infection during protein deficiency.

    abstract::Balb/c mice infected with the gastrointestinal nematode Heligmosomoides bakeri were fed protein sufficient (PS, 24%) or deficient (PD, 3%) diets to investigate whether diet, infection or dose of larval challenge (0, 100 or 200 larvae) influenced gut pathophysiology and inflammation. Among the PS mice, worms were more ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182007003617

    authors: Tu T,Koski KG,Scott ME

    更新日期:2008-01-01 00:00:00

  • Determinants of life-history traits in a fish ectoparasite: a hierarchical analysis.

    abstract:OBJECTIVE:Unravelling the determinants of parasite life-history traits in natural settings is complex. Here, we deciphered the relationships between biotic, abiotic factors and the variation in 4 life-history traits (body size, egg presence, egg number and egg size) in the fish ectoparasite Tracheliastes polycolpus. We...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201100014X

    authors: Loot G,Poulet N,Brosse S,Tudesque L,Thomas F,Blanchet S

    更新日期:2011-06-01 00:00:00

  • Differentially expressed parasite genes involved in host recognition and invasion of the triactinomyxon stage of Myxobolus cerebralis (Myxozoa).

    abstract::The host recognition and invasion process of Myxobolus cerebralis actinospores (triactinomyxon, TAM) was studied on a genetic level. A small-scale in vitro assay was developed to activate a large number of TAMs simultaneously, and to monitor the host invasion in the absence of live fish. The transcriptomes of non-acti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008005398

    authors: Eszterbauer E,Kallert DM,Grabner D,El-Matbouli M

    更新日期:2009-04-01 00:00:00

  • Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematoda).

    abstract::The tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and within 3 h host plas...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000065513

    authors: Bayne CJ,Loker ES,Yui MA

    更新日期:1986-06-01 00:00:00

  • Exposure of small mammals to ticks and rickettsiae in Atlantic Forest patches in the metropolitan area of Recife, North-eastern Brazil.

    abstract::Between December 2007 and March 2009, small mammals were captured in 6 Atlantic Forest patches in Brazil. We assessed tick-host associations and whether they differ among forest strata, sites, seasons, and host age classes or between sexes. Moreover, we assessed the exposure of animals to Rickettsia spp. In total, 432...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011001740

    authors: Dantas-Torres F,Aléssio FM,Siqueira DB,Mauffrey JF,Marvulo MF,Martins TF,Moraes-Filho J,Camargo MC,D'Auria SR,Labruna MB,Silva JC

    更新日期:2012-01-01 00:00:00

  • Immunologically mediated, non-specific interactions between the intestinal phases of Trichinella spiralis and Nippostrongylus brasiliensis in the mouse.

    abstract::Interactions between infections of Trichinella spiralis and Nippostrongylus brasiliensis were studied in the NIH strain of mouse which is known to react strongly to T. spiralis. The course of N. brasiliensis infection in this strain of mouse is described and expulsion is shown to be accelerated in immunized mice and i...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000000512

    authors: Kennedy MW

    更新日期:1980-02-01 00:00:00

  • Maternal transfer of antibodies induced by infection with Eimeria maxima partially protects chickens against challenge with Eimeria tenella.

    abstract::Infection of breeding hens with Eimeria maxima induces production of Eimeria-specific IgG antibodies which are transferred to hatchlings via the egg yolk and confer a high degree of maternal immunity against homologous challenge and partial immunity to infection with another important species, Eimeria tenella. As an e...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000076423

    authors: Smith NC,Wallach M,Petracca M,Braun R,Eckert J

    更新日期:1994-12-01 00:00:00

  • Fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae of Ascaris suum.

    abstract::Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process of Ascaris suum larvae. This study describes moulting and development-specific protein expression profiles of A. suum lung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepa...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006000576

    authors: Islam MK,Miyoshi T,Yamada M,Alim MA,Huang X,Motobu M,Tsuji N

    更新日期:2006-10-01 00:00:00

  • When they don't bite, we smell money: understanding malaria bednet misuse.

    abstract::Insecticide-treated nets (ITNs) are a major tool to control malaria. Over recent years increased ITN coverage has been associated with decreased malaria transmission. However, ITN 'misuse' has been increasingly reported and whether this emergent behaviour poses a threat to successful malaria control and elimination is...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182012002077

    authors: Honjo K,Chaves LF,Satake A,Kaneko A,Minakawa N

    更新日期:2013-04-01 00:00:00

  • Infection success of different trematode genotypes in two alternative intermediate hosts: evidence for intraspecific specialization?

    abstract::The evolution of host specificity and the potential trade-off between being a generalist and a specialist are central issues in the evolutionary ecology of parasites. Different species of parasites or even different populations of the same species often show different degrees of host specificity. However, less is know...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009991107

    authors: Leung TL,Poulin R

    更新日期:2010-02-01 00:00:00

  • Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences.

    abstract::The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000067263

    authors: Allsopp BA,Baylis HA,Allsopp MT,Cavalier-Smith T,Bishop RP,Carrington DM,Sohanpal B,Spooner P

    更新日期:1993-08-01 00:00:00

  • Philasterides dicentrarchi (Ciliophora:Scuticociliatida) expresses surface immobilization antigens that probably induce protective immune responses in turbot.

    abstract::Philasterides dicentrarchi is a histophagous ciliate causing systemic scuticociliatosis in cultured turbot. This study demonstrates that turbot which survive this disease have serum antibodies that recognize ciliary antigens of this ciliate in ELISA and immobilize/agglutinate the ciliate in vitro. Mouse sera raised ag...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182002002688

    authors: Iglesias R,Paramá A,Alvarez MF,Leiro J,Ubeira FM,Sanmartín ML

    更新日期:2003-02-01 00:00:00

  • Theileria parva live vaccination: parasite transmission, persistence and heterologous challenge in the field.

    abstract::The 'Muguga cocktail' live vaccine, delivered by an infection and treatment protocol, has been widely deployed in Eastern, Central and Southern Africa to protect cattle against East Coast fever, caused by Theileria parva. The vaccine contains 3 component stocks (Muguga, Serengeti-transformed and Kiambu 5). In a previo...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182007002557

    authors: Oura CA,Bishop R,Asiimwe BB,Spooner P,Lubega GW,Tait A

    更新日期:2007-08-01 00:00:00

  • Lung-worms (Nematoda: Pseudaliidae) of harbour porpoises (Phocoena phocoena) in Norwegian waters: patterns of colonization.

    abstract::We studied the component community of lung-worms of the harbour porpoise, attempting to establish the relative importance of ecological and evolutionary factors on its development. The lungs of 64 porpoises by-caught in Norwegian waters were examined for helminths. Three pseudaliid species were detected. The porpoises...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000076186

    authors: Balbuena JA,Aspholm PE,Andersen KI,Bjørge A

    更新日期:1994-04-01 00:00:00

  • Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica.

    abstract::Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet ge...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000061679

    authors: Cruz-Reyes JA,Spice WM,Rehman T,Gisborne E,Ackers JP

    更新日期:1992-04-01 00:00:00

  • Carbohydrate inhibition of Biomphalaria glabrata embryonic (Bge) cell adhesion to primary sporocysts of Schistosoma mansoni.

    abstract::Due to shared characteristics with snail haemocytes, the Biomphalaria glabrata embryonic (Bge) cell line has been used as a model in vitro system for the study of snail-trematode interactions. In this study, Bge cells were used to characterize the adherence of snail host cells to schistosome primary sporocysts, and to...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:

    authors: Castillo MG,Yoshino TP

    更新日期:2002-12-01 00:00:00

  • Time of treatment influences the appearance of drug-resistant parasites in Plasmodium falciparum infections.

    abstract::A deterministic mathematical model which predicts the probability of developing a new drug-resistant parasite population within the human host is reported. The model incorporates the host's specific antibody response to PfEMP1, and also investigates the influence of chemotherapy on the probability of developing a viab...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001008824

    authors: Gatton ML,Hogarth W,Saul A

    更新日期:2001-12-01 00:00:00

  • In vitro influence of Theileria annulata on the functions of bovine dendritic cells for stimulation of T lymphocyte proliferation.

    abstract::The present study was performed on antigen-presenting cells (APCs) of Theileria annulata transformed dendritic cells (TaDCs) and monocyte-derived dendritic cells (MoDCs) to compare differences in antigen presentation and stimulation of T lymphocyte proliferation. Antigen presentation for T lymphocyte proliferation was...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182019001227

    authors: Rashid M,Liu J,Guan G,Wang J,Li Z,Hassan MA,Rashid MI,Mukhtar MU,Luo J,Yin H

    更新日期:2020-01-01 00:00:00

  • An egg-adapted (attenuated) line of Eimeria necatrix: further studies on its reproduction, pathogenicity and immunogenicity.

    abstract::An egg-adapted line of Eimeria necatrix has now been passaged 40 times in the chorio-allantoic membranes of embryonated eggs. Between the 21st and 40th passage in eggs the parasite was subjected to a selection for precocious development, and its pre-patent period in this host is now 123-125 h. The parasite continued t...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000044784

    authors: Shirley MW,Bellatti MA,Millard BJ

    更新日期:1982-04-01 00:00:00

  • Behaviour and development of Elasmosoma sp. (Neoneurinae: Braconidae: Hymenoptera), an endoparasite of Formica ants (Formicidae: Hymenoptera).

    abstract::The behaviour and development of Elasmosoma sp., near pergandei Ashm. (Neoneurinae: Braconidae: Hymenoptera), a parasite of the ant, Formica obscuriventris clivia Creighton, was studied in a coastal sand dune habitat in central Oregon over a 2-year period. Female wasps oviposited into the abdomen of major worker ants ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004004809

    authors: Poinar G Jr

    更新日期:2004-05-01 00:00:00

  • Infections with Eimeria maxima and Eimeria acervulina in the fowl: effect of previous infection with the heterologous organism on oocyst production.

    abstract::Judged by oocyst production, infections with Eimeria acervulina in birds immunized with E. maxima were consistently higher than in birds which had not been immunized. Oocyst production of E. maxima in birds previously infected with E. acervulina was greater, in three out of four experiments, than in control chickens,...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000049726

    authors: Rose ME

    更新日期:1975-04-01 00:00:00

  • Dynamics of Sept4 expression in fibrotic livers of mice infected with Schistosoma japonicum.

    abstract::n order to investigate the dynamics of Septin4 (Sept4) expression and its function in the formation of fibrotic livers in mice infected with Schistosoma japonicum, we constructed the mouse model of S. japonicum egg-induced liver fibrosis for 24 weeks. Immunohistochemical staining, qRT-PCR and Western blot were used to...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011000667

    authors: Duan YN,Qian HY,Qin YW,Zhu DD,He XX,Zhou Q,Yang YN,Bao J,Feng JR,Sun W,Chen JL

    更新日期:2011-07-01 00:00:00

  • Host-parasite relationship between congenital Toxoplasma infection and mouse brain: role of small vessels.

    abstract::Small vessels showing inflammatory cell infiltrates were invariably observed in the vicinity of intact Toxoplasma tissue cysts within the brains of mice congenitally infected with the protozoan. Lymphocytes were observed in intimate contact with the luminal aspect of the endothelium, penetrating into the thickened bas...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063873

    authors: Sims TA,Hay J

    更新日期:1995-02-01 00:00:00

  • Immunocytochemical demonstration of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica (Trematoda, Digenea).

    abstract::The localization and distribution of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica at different stages in the development of the adult fluke have been determined by an indirect immunofluorescence technique, using antisera to 19 vertebrate peptides and the invertebrate neuropeptide, FMRFamid...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000062132

    authors: Magee RM,Fairweather I,Johnston CF,Halton DW,Shaw C

    更新日期:1989-04-01 00:00:00