Allosteric control of pyrimidine transport in Hymenolepis diminuta: an unusual kinetic isotope effect.

Abstract:

:A thymine-uracil transport system is present in the plasma membrane brush-border of the tapeworm, Hymenolepis diminuta. The relation between initial uptake and substrate concentration of either thymine or uracil was sigmoidal when 2-14C-labelled substrates were used. In contrast, absorption kinetics of methyl-14C- and 3H-labelled substrates were hyperbolic. Since there was no metabolism of the labelled substrates during the incubation period, these differences indicate that the sigmoidal kinetics of pyrimidine transport in this organism is an isotopic effect associated with the presence of the 14C label at the 2 position in the pyrimidine ring.

journal_name

Parasitology

journal_title

Parasitology

authors

Uglem GL,Dupre RK,Harley JP

doi

10.1017/s0031182000052641

subject

Has Abstract

pub_date

1983-10-01 00:00:00

pages

289-93

eissn

0031-1820

issn

1469-8161

journal_volume

87 (Pt 2)

pub_type

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