Abstract:
:The free radical nitric oxide (NO) is a neuronal messenger which is synthesized from L-arginine and O2 by nitric oxide synthase (NOS). In the synthesis NO and L-citrulline are produced in a stoichiometric 1:1 relation. The activity of NOS was analysed in homogenates of the rat tapeworm Hymenolepis diminuta by measuring the formation of L-[3H]citrulline after incubation with L-[3H]arginine. The nature of NOS in H. diminuta was determined by studying the effect of 3 types of NOS inhibitors: (1) L-NAME, (2) EGTA, (3) 7-nitro-indazole. All inhibitors caused a significant but not complete reduction in the formation of L-[3H]citrulline. The results are discussed against the background of nerve cells and fibres positive for NADPH-diaphorase staining in H. diminuta.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Terenina NB,Onufriev MV,Gulyaeva NV,Lindholm AM,Gustafsson MKdoi
10.1017/s0031182099005284subject
Has Abstractpub_date
2000-01-01 00:00:00pages
91-5eissn
0031-1820issn
1469-8161journal_volume
120 ( Pt 1)pub_type
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