Abstract:
:The transmission-blocking monoclonal antibody 13.1, which recognizes the ookinete surface antigen Pbs21 of Plasmodium berghei, and an IgG2a isotype control antibody 26.37 were purified by caprylic acid and ammonium sulphate precipitation. Fab fragments were prepared by papain digestion. IgG but not Fab from antibody 13.1 reduced ookinete formation by P. berghei in culture by as much as 94% at a concentration of 100 micrograms/ml. There was little difference in antibody efficacy in the range 6.25-400 micrograms/ml in this assay. The parasite was most sensitive to antibody activity in the first 6-9 h of culture, i.e. the gamete/zygote and early retort stages. Peripheral blood leucocytes (PBL) were essential to achieve maximal inhibition by mAb 13.1 (activity was abrogated totally if PBL were removed). Together the data suggest that one of the mechanisms of action of this antibody is antibody-mediated PBL killing. Phagocytosis of parasites was noted in these experiments in all cultures. We have not attempted in this study to distinguish between Fc-mediated opsonization, as opposed to antibody-dependent cellular cytotoxicity.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Ranawaka GR,Alejo-Blanco AR,Sinden REdoi
10.1017/s0031182000077702subject
Has Abstractpub_date
1994-07-01 00:00:00pages
11-7eissn
0031-1820issn
1469-8161journal_volume
109 ( Pt 1)pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::SSU ribosomal sequences of trypanosomes from Brazilian cattle and water buffalo were used to infer phylogenetic relationships between non-pathogenic T. theileri and allied species parasitic in artiodactyls. T. theileri trypanosomes from distinct geographical regions in Brazil and from other countries were tightly clus...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008929
更新日期:2006-02-01 00:00:00
abstract::In this study, we describe the molecular analysis of zinc-metalloproteases from the abomasal nematode Ostertagia ostertagi which were exclusively recognized by local antibodies of immune cattle. Full-length or partial coding sequences of 4 different zinc-metalloprotease cDNAs of Ostertagia (met-1, -2, -3 and -4) were ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006274
更新日期:2005-01-01 00:00:00
abstract::Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009990837
更新日期:2010-01-01 00:00:00
abstract::Species composition of wild reservoir hosts can influence the transmission and maintenance of multi-host vector borne pathogens. The 'pace of life' hypothesis proposes that the life history strategy of reservoir hosts can influence pathogen transmission of vector borne generalist pathogens. We use empirical data to pa...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017001287
更新日期:2017-12-01 00:00:00
abstract::The dynamic interaction of cells of the immune system with other cells, antigens and secreted factors determines the nature of an immune response. The response of individual cells is governed by the sequence of intracellular signalling events triggered following the association of cell surface molecules during cell-ce...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182005008115
更新日期:2005-01-01 00:00:00
abstract::Cod (Gadus morhua), an important fish species in the Baltic Sea, is the paratenic host for many parasite species, including the zoonotic nematodes, Anisakis sp. and Contracaecum osculatum. We aimed to identify which invertebrate species (found in situ in the fish stomach) are responsible for infection of cod with zoon...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019001124
更新日期:2019-11-01 00:00:00
abstract::A specific enzyme-linked immunosorbent assay, based on a 48 kDa metallo-proteinase isolated from the frontal glands of a porocephalid pentastomid (Porocephalus crotali) in rat intermediate hosts, is described. SDS-PAGE analysis of whole nymphal homogenate, followed by Western blotting and probing with immune rat serum...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000059849
更新日期:1991-12-01 00:00:00
abstract::Why do parasites kill their hosts? During this past decade, research in three different areas; evolutionary ecology, medical microbiology, and population genetics has provided theory and data that address this and related questions of selection and the evolution and maintenance of parasite virulence. A general theory ...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000073054
更新日期:1990-01-01 00:00:00
abstract::Feulgen-pararosaniline (SO2) staining was performed on stages in the life-cycle of Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei. The fluorescence emission of the stained DNA in nuclei of these stages was examined and compared with absorption microscopy measurements at 560 nm ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000054305
更新日期:1984-02-01 00:00:00
abstract::A one-year study of gastro-intestinal parasitism in a free-ranging population of maras at Whipsnade Wild Animal Park, UK, revealed a strong relationship between membership of social units and both intensity and prevalence of infection. The mara, a hystricomorph rodent from southern Argentina, has a social organization...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182097002205
更新日期:1998-03-01 00:00:00
abstract::A distinct 8 kDa calcium-binding protein (CaBP) is preferentially expressed at the cercarial stage during the life-cycle of the schistosome. Available data indicate that this CaBP may be associated with tissue/organ remodelling (involving protein degradation and synthesis of new proteins) during transformation of the ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182003003858
更新日期:2003-10-01 00:00:00
abstract::A field trial was conducted to evaluate the potential of the nematode-destroying fungus Duddingtonia flagrans to control free-living stages of horse strongyles. In late Spring 2 groups of horses (yearlings) with mixed infections of strongyles were allowed to contaminate 2 equal-sized pastures. One of the groups (F) re...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200006621x
更新日期:1996-07-01 00:00:00
abstract::A prediction of the hypothesis of Wilson (1977, 1980 a, b) to account for larval migration of homogonic Strongyloides ratti in the host is that the pattern of invasion of the mammary gland of a lactating rat will be quantitatively similar on both sides and independent of the point of entry into the body. Twenty-one su...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000052744
更新日期:1982-06-01 00:00:00
abstract::Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of p...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008504
更新日期:2005-12-01 00:00:00
abstract::Factors which may be important in the large-scale extraction of coccidial oocysts from faeces ha.ve been investigated with Eimeria tenella. Age of bird, inoculum, feeding status at the time of inoculation, period of collection, feeding status during collection, collection medium, homogenization and sieving, flotation,...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000046990
更新日期:1976-12-01 00:00:00
abstract::The localization and distribution of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica at different stages in the development of the adult fluke have been determined by an indirect immunofluorescence technique, using antisera to 19 vertebrate peptides and the invertebrate neuropeptide, FMRFamid...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000062132
更新日期:1989-04-01 00:00:00
abstract::If the deleterious effects of non-specific parasites are greater on vulnerable host species than on reservoir host species then exclusion of the vulnerable host through apparent competition is more likely. Evidence suggests that such a mechanism occurs in interactions between the ring-necked pheasant (Phasianus colchi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001007247
更新日期:2001-02-01 00:00:00
abstract::Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for th...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006000898
更新日期:2006-11-01 00:00:00
abstract::Immunization against the protozoan Theileria parva by infection and treatment has proved to be very efficient for the control of East Coast fever, an acute and often-fatal lymphoproliferative tick-borne disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. parva sporozoites used in ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182003004323
更新日期:2004-02-01 00:00:00
abstract::Differentiation of epimastigotes and production of infective metacyclic forms of Trypanosoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them...
journal_title:Parasitology
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::The community structure of monogeneans on the gills of a catfish Pimelodus albicans, in the Río de la Plata, was studied and compared with previous findings on P. maulatus. Six Monogenea species belonging to 3 genera were found: Demidospermus majusculus Kritsky & Gutiérrez, 1998; Demidospermus armostus Kritsky & Gutié...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200100748x
更新日期:2001-04-01 00:00:00
abstract::Aiming to further characterize the haemocyte subsets in Biomphalaria snails, we have performed a detailed flow cytometric analysis of whole haemolymph cellular components using a multiparametric dual colour labelling procedure. Ethidium bromide/acridine orange fluorescence features were used to first select viable hae...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008005155
更新日期:2009-01-01 00:00:00
abstract::We have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangi and Dirofilaria imitis) and of the free-living soil nematodes Caenorhabditis elegans and have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal dena...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000066750
更新日期:1988-08-01 00:00:00
abstract::The glycolytic enzyme triosephosphate isomerase catalyses the isomerization between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Here we report that Trichomonas vaginalis contains 2 fully functional tpi genes. Both genes are located in separated chromosomal context with different promoter regulatory elem...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118201200114X
更新日期:2012-11-01 00:00:00
abstract::Schistosoma mansoni depends for its survival on glycolysis. Two glycolytic enzymes, glyceraldehyde-3P-dehydrogenase and triose-phosphate dehydrogenase, found in both the adult and schistosomular tegument, have been reported to confer partial protection against cercarial infection. This paper describes the immunogenic ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099005648
更新日期:2000-05-01 00:00:00
abstract::Malaria remains a major cause of human morbidity and mortality worldwide. Plasmodium falciparum, the most virulent of the 4 human Plasmodium species causing malaria, is potentially life threatening, is increasing in prevalence and is becoming even more resistant to in-use drugs. In light of the growing problem of mult...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008267
更新日期:2005-10-01 00:00:00
abstract::Blastocystis SSU-rDNA sequence data from 317 captive and free-living non-human primates (NHPs) representing 30 genera of apes, Old and New World (OW and NW) monkeys and prosimians were analysed to investigate subtype (ST) and allele distribution among hosts. Excluding 20 mixed ST infections, 27% of the sequences belon...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013000255
更新日期:2013-07-01 00:00:00
abstract::The Calliphoridae include some of the most economically significant myiasis-causing flies in the world - blowflies and screwworm flies - with many being notorious for their parasitism of livestock. However, despite more than 50 years of research, key taxonomic relationships within the family remain unresolved. This st...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001089
更新日期:2011-11-01 00:00:00
abstract::Bat flies are obligate ectoparasites of bats and it has been hypothesized that they may be involved in the transmission of Bartonella species between bats. A survey was conducted to identify whether Cyclopodia greefi greefi (Diptera: Nycteribiidae) collected from Ghana and 2 islands in the Gulf of Guinea harbour Barto...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011002113
更新日期:2012-03-01 00:00:00
abstract::The pre-feeding and feeding periods of larvae, nymphs and adults of Rhipicephalus appendiculatus on rabbits (Oryctolagus cuniculus) were investigated. Larvae and nymphs required at least 8-9 days after hatching and moulting respectively before they could attach and start feeding, while adults required at least 6-9 day...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000043195
更新日期:1976-02-01 00:00:00