Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system.

Abstract:

:Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed over time using electron microscopy. Additional data are presented on the morphology, development and behavioural characteristics of the different life-cycle stages as well as determining their time of occurrence after inoculation. Numerous stages of C. parvum and their behaviour have been visualized and morphologically characterized for the first time using scanning electron microscopy. Further, parasite-host interactions and the effect of C. parvum on host cells were also visualized. An improved understanding of the parasite's biology, proliferation and interactions with host cells will aid in the development of treatments for the disease.

journal_name

Parasitology

journal_title

Parasitology

authors

Borowski H,Thompson RC,Armstrong T,Clode PL

doi

10.1017/S0031182009990837

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

13-26

issue

1

eissn

0031-1820

issn

1469-8161

pii

S0031182009990837

journal_volume

137

pub_type

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