A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples.

Abstract:

:Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94.4% sensitivity and 99.9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100% sensitivity and 98.9% specificity in a species-specific (S. mansoni) PCR.

journal_name

Parasitology

journal_title

Parasitology

authors

Sandoval N,Siles-Lucas M,Pérez-Arellano JL,Carranza C,Puente S,López-Abán J,Muro A

doi

10.1017/S0031182006000898

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

581-7

issue

Pt 5

eissn

0031-1820

issn

1469-8161

pii

S0031182006000898

journal_volume

133

pub_type

杂志文章
  • Substrate specificity of the neutral sphingomyelinase from Trypanosoma brucei.

    abstract::The kinetoplastid parasite Trypanosoma brucei causes African trypanosomiasis in both humans and animals. Infections place a significant health and economic burden on developing nations in sub-Saharan Africa, but few effective anti-parasitic treatments are currently available. Hence, there is an urgent need to identify...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182018001853

    authors: Dickie EA,Young SA,Smith TK

    更新日期:2019-04-01 00:00:00

  • Immunolocalization and characterization of the low molecular weight antigen (4-5 kDa) of Toxoplasma gondii that elicits an early IgM response upon primary infection.

    abstract::A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonst...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000068220

    authors: Tomavo S,Couvreur G,Leriche MA,Sadak A,Achbarou A,Fortier B,Dubremetz JF

    更新日期:1994-02-01 00:00:00

  • What has proteomics taught us about Leishmania development?

    abstract::Leishmania are obligatory intracellular parasitic protozoa that cycle between sand fly mid-gut and phagolysosomes of mammalian macrophages. They have developed genetically programmed changes in gene and protein expression that enable rapid optimization of cell function according to vector and host environments. During...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182012000157

    authors: Tsigankov P,Gherardini PF,Helmer-Citterich M,Zilberstein D

    更新日期:2012-08-01 00:00:00

  • Superoxide dismutase (SOD) activity of Dictyocaulus viviparus and its inhibition by antibody from infected and vaccinated bovine hosts.

    abstract::The presence of superoxide dismutase (SOD) activity in the bovine lungworm Dictyocaulus viviparus was examined using the xanthine-xanthine oxidase assay system and by non-denaturing PAGE followed by specific enzyme staining. High levels of activity were detected in excretory-secretory (ES) products of adult worms and ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000076381

    authors: Britton C,Knox DP,Kennedy MW

    更新日期:1994-08-01 00:00:00

  • Pathophysiology of parasitic infections.

    abstract::Parasites can have a wide range of pathophysiological effects on the host. This review describes those associated with some parasites of major importance in man and animals. Haemoprotozoan diseases such as trypanosomiasis and malaria are primarily associated with anaemia. Such anaemias have a complex aetiology involvi...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182000085814

    authors: Holmes PH

    更新日期:1987-01-01 00:00:00

  • Strongyloides ratti: studies of 75Se-labelled larvae of the homogonic strain in female hosts.

    abstract::A prediction of the hypothesis of Wilson (1977, 1980 a, b) to account for larval migration of homogonic Strongyloides ratti in the host is that the pattern of invasion of the mammary gland of a lactating rat will be quantitatively similar on both sides and independent of the point of entry into the body. Twenty-one su...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000052744

    authors: Wilson PA,Simpson NE

    更新日期:1982-06-01 00:00:00

  • Enzymatic amplification of mini-exon-derived RNA gene spacers of Leishmania donovani: primers and probes for DNA diagnosis.

    abstract::The multicopy mini-exon-derived RNA (med RNA) locus of Leishmania donovani was enzymatically amplified by the polymerase chain reaction (PCR). The major 180 bp PCR product contained conserved med RNA gene sequences flanking the variable intergenic spacer from the med RNA gene tandem repeat. The oligonucleotide primers...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000068086

    authors: Hassan MQ,Ghosh A,Ghosh SS,Gupta M,Basu D,Mallik KK,Adhya S

    更新日期:1993-12-01 00:00:00

  • Insights on adaptive and innate immunity in canine leishmaniosis.

    abstract::Canine leishmaniosis (CanL) is caused by the parasite Leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. Clinical manifestations can range from subclinical infection to very severe systemic disease. Leishmaniosis is categorized as a neg...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201600055X

    authors: Hosein S,Blake DP,Solano-Gallego L

    更新日期:2017-01-01 00:00:00

  • Protein synthesis and release by cultured schistosomula of Schistosoma mansoni.

    abstract::The lung schistosomulum of Schistosoma mansoni is the target of protective immunity in mice singly vaccinated with irradiated cercariae. Since the effector responses are T cell-mediated, their initiation requires the release of antigens from the intact parasite. We have used the technique of biosynthetic labelling wit...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000079245

    authors: Harrop R,Wilson RA

    更新日期:1993-09-01 00:00:00

  • A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts.

    abstract::Infection with the endosymbiotic bacteria Wolbachia is widespread in filarial nematodes. Previous studies have suggested concordance between the phylogeny of Wolbachia with that of their nematode hosts. However, there is only one published molecular phylogenetic study of filarial species, based on the 5S rRNA gene spa...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000007149

    authors: Casiraghi M,Anderson TJ,Bandi C,Bazzocchi C,Genchi C

    更新日期:2001-01-01 00:00:00

  • Depletion of the ozone layer: consequences for non-infectious human diseases.

    abstract::Stratospheric ozone depletion threatens to increase exposure to ultraviolet (UV) radiation which is known to be a factor in a number of diseases. There is little doubt that cumulative exposure to UV radiation is important in the aetiology of non-melanoma skin cancers. Evidence is also strong for a link with cutaneous ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182000086108

    authors: Bentham G

    更新日期:1993-01-01 00:00:00

  • Biological control of ticks.

    abstract::Ticks have numerous natural enemies, but only a few species have been evaluated as tick biocontrol agents (BCAs). Some laboratory results suggest that several bacteria are pathogenic to ticks, but their mode of action and their potential value as biocontrol agents remain to be determined. The most promising entomopath...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182004005219

    authors: Samish M,Ginsberg H,Glazer I

    更新日期:2004-01-01 00:00:00

  • A new thrombospondin-related anonymous protein homologue in Neospora caninum (NcMIC2-like1).

    abstract::Neospora caninum is an Apicomplexan protozoan that has the dog as a definitive host and cattle (among other animals) as intermediate hosts. It causes encephalopathy in dogs and abortion in cows, with significant loss in worldwide livestock. As any Apicomplexan, the parasite invades the cells using proteins contained i...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010001290

    authors: Pereira LM,Candido-Silva JA,De Vries E,Yatsuda AP

    更新日期:2011-03-01 00:00:00

  • Identification and diagnosis of Leishmania mexicana complex isolates by polymerase chain reaction.

    abstract::Following cloning of Leishmania (L.) amazonensis kinetoplast DNA two recombinant clones were identified: one specific for L. (L.) amazonensis and the other specific for L. (L.) amazonensis and closely related isolates. DNA sequences from these clones were compared with those of other kinetoplastids and oligonucleotide...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000080677

    authors: Eresh S,McCallum SM,Barker DC

    更新日期:1994-11-01 00:00:00

  • Mesocestoides corti intracranial infection as a murine model for neurocysticercosis.

    abstract::Neurocysticercosis (NCC) is the most common parasitic disease of the central nervous system (CNS) caused by the larval form of the tapeworm Taenia solium. NCC has a long asymptomatic period with little or no inflammation, and the sequential progression to symptomatic NCC depends upon the intense inflammation associate...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182009991971

    authors: Alvarez JI,Mishra BB,Gundra UM,Mishra PK,Teale JM

    更新日期:2010-03-01 00:00:00

  • Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies.

    abstract::Antigenic differences between intra-lymphocytic theilerial parasites isolated from the blood of 18 African buffalo and grown in vitro were assessed with anti-schizont monoclonal antibodies (mAbs). There was marked antigenic diversity both between isolates from different buffalo and between isolates taken at different ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055761

    authors: Conrad PA,Stagg DA,Grootenhuis JG,Irvin AD,Newson J,Njamunggeh RE,Rossiter PB,Young AS

    更新日期:1987-06-01 00:00:00

  • Anthelmintic activity of plants against gastrointestinal nematodes of goats: a review.

    abstract::The gastrointestinal nematodes (GIN) stand out as an important cause of disease in small ruminant, especially on goat farm. Widespread resistance to synthetic anthelminthics has stimulated the research for alternative strategies of parasite control, including the use of medicinal plants. The present work summarizes th...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182019000672

    authors: Oliveira Santos F,Ponce Morais Cerqueira A,Branco A,José Moreira Batatinha M,Borges Botura M

    更新日期:2019-09-01 00:00:00

  • Characterization of an atypical antigen from Sarcoptes scabiei containing an MADF domain.

    abstract::We have cloned a cDNA encoding a novel antigen from a Sarcoptes scabiei (Acari) cDNA library by immunoscreening with sera from S. scabiei-infected dogs. The antigen is encoded by a 2,157 bp mRNA with a predicted open reading frame of 719 amino acids (molecular weight 79 kDa). Our sequence analysis identified the prese...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005008747

    authors: Ljunggren EL,Bergström K,Morrison DA,Mattsson JG

    更新日期:2006-01-01 00:00:00

  • Loss of behavioural dominance in male mice infected with Trichinella spiralis.

    abstract::Trichinella spiralis infections may lead to the loss of behavioural dominance among male mice. Reversals of dominance order first appear at the time when newborn larvae are released into the circulation of the host. The duration of dominance reversals bears no relationship to the number of muscle larvae harboured. ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:

    authors: Rau ME

    更新日期:1984-04-01 00:00:00

  • Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study.

    abstract::The recent development of genetic methods allows the delineation of species boundaries, especially in organisms where morphological characters are not reliable to differentiate species. However, few empirical studies have used these tools to delineate species among parasitic metazoans. Here we investigate the species ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001517

    authors: Pérez-Ponce DE León G,García-Varela M,Pinacho-Pinacho CD,Sereno-Uribe AL,Poulin R

    更新日期:2016-11-01 00:00:00

  • Characterization of Theileria parva which infects waterbuck (Kobus defassa).

    abstract::Theileria-free waterbuck (Kobus defassa) born in captivity were successfully infected with Theileria parva sporozoites derived from ticks infected by feeding on African buffalo (Syncerus caffer). All waterbuck underwent mild infections with the development of sporadic schizont and piroplasm parasitosis when inoculated...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000077416

    authors: Stagg DA,Bishop RP,Morzaria SP,Shaw MK,Wesonga D,Orinda GO,Grootenhuis JG,Molyneux DH,Young AS

    更新日期:1994-06-01 00:00:00

  • Mutual dilution of infection by an introduced parasite in native and invasive stream fishes across Hawaii.

    abstract::The presence of introduced hosts can increase or decrease infections of co-introduced parasites in native species of conservation concern. In this study, we compared parasite abundance, intensity, and prevalence between native Awaous stamineus and introduced poeciliid fishes by a co-introduced nematode parasite (Camal...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001001

    authors: Gagne RB,Heins DC,McIntyre PB,Gilliam JF,Blum MJ

    更新日期:2016-10-01 00:00:00

  • Host associations and turnover of haemosporidian parasites in manakins (Aves: Pipridae).

    abstract::Parasites of the genera Plasmodium and Haemoproteus (Apicomplexa: Haemosporida) are a diverse group of pathogens that infect birds nearly worldwide. Despite their ubiquity, the ecological and evolutionary factors that shape the diversity and distribution of these protozoan parasites among avian communities and geograp...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017000208

    authors: Fecchio A,Svensson-Coelho M,Bell J,Ellis VA,Medeiros MC,Trisos CH,Blake JG,Loiselle BA,Tobias JA,Fanti R,Coffey ED,DE Faria IP,Pinho JB,Felix G,Braga EM,Anciães M,Tkach V,Bates J,Witt C,Weckstein JD,Ricklefs RE,

    更新日期:2017-06-01 00:00:00

  • Changes in the zymogenic cell populations of the abomasa of sheep infected with Haemonchus contortus.

    abstract::The effects of dietary urea supplementation and of a 10-week trickle infection regime, simulating chronic exposure to Haemonchus contortus, on the zymogenic population of the abomasa of Hampshire Down lambs was examined. At necropsy a variety of parameters including plasma pepsinogen concentration, the wet weights of ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182098002704

    authors: Scott I,Stear MJ,Irvine J,Dick A,Wallace DS,McKellar QA

    更新日期:1998-06-01 00:00:00

  • Development of larval Echinococcus granulosus confronted with chicken heart tissue in vitro.

    abstract::Vesiculated protoscoleces (VP) were produced by culturing freshly collected protoscoleces from Echinococcus granulosus horse liver hydatids in RPMI 1640 monophasic medium at 37 degrees C for 18 days. Half of the VP were used as such, the other half used after killing them by freeze-thawing. Nine-day-old chicken heart ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000078756

    authors: Janssen D,De Rycke PH,De Ridder L

    更新日期:1990-06-01 00:00:00

  • Nitric oxide interaction with IL-10, MIP-1alpha, MCP-1 and RANTES over the in vitro granuloma formation against different Schistosoma mansoni antigenic preparations on human schistosomiasis.

    abstract::Nitric oxide (NO) produced by cytokine-activated macrophages is reported to be cytotoxic against the helminth Schistosoma mansoni, although this is a controversial issue. Previous work in our laboratory identified a fraction of S. mansoni soluble adult worm antigenic preparation (SWAP), named PIII, able to elicit sign...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005636

    authors: Oliveira DM,Silva-Teixeira DN,Gustavson S,Oliveira SM,Goes AM

    更新日期:2000-04-01 00:00:00

  • Analysis of aggregation, a worked example: numbers of ticks on red grouse chicks.

    abstract::The statistical aggregation of parasites among hosts is often described empirically by the negative binomial (Poisson-gamma) distribution. Alternatively, the Poisson-lognormal model can be used. This has the advantage that it can be fitted as a generalized linear mixed model, thereby quantifying the sources of aggrega...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001007740

    authors: Elston DA,Moss R,Boulinier T,Arrowsmith C,Lambin X

    更新日期:2001-05-01 00:00:00

  • Epidemiology and optimal foraging: modelling the ideal free distribution of insect vectors.

    abstract::Existing models of the basic case reproduction number (R0) for vector-borne diseases assume (i) that the distribution of vectors over the susceptible host species is homogeneous and (ii) that the biting preference for the susceptible host species rather than other potential hosts is a constant. Empirical evidence cont...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005442

    authors: Kelly DW,Thompson CE

    更新日期:2000-03-01 00:00:00

  • Characterization of Meloidogyne javanica surface coat with antibodies and their effect on nematode behaviour.

    abstract::The surface coat of the 2nd-stage juveniles (J2) of plant-parasitic nematodes is considered to be involved in interactions with microorganisms in the soil and rhizosphere, as well as with the host plant. Characterization of surface antigens might be important in the development of new nematode control strategies. In t...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182002001907

    authors: Sharon E,Spiegel Y,Salomon R,Curtis RH

    更新日期:2002-08-01 00:00:00

  • Non-specific induction of increased resistance in mice to Trypanosoma congolense and Trypanosoma brucei by immunostimulants.

    abstract::Administration of the immunostimulants Corynebacterium parvum, Bacillus Calmette-Guérin (BCG) or Bordetella pertussis prior to, or at the same time as, challenge with Trypanosoma congolense significantly increased survival times in mice, both of trypano-susceptible (A/J) and trypano-resistant (C57Bl) strains. The incr...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000053750

    authors: Murray M,Morrison WI

    更新日期:1979-12-01 00:00:00