Abstract:
:Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94.4% sensitivity and 99.9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100% sensitivity and 98.9% specificity in a species-specific (S. mansoni) PCR.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Sandoval N,Siles-Lucas M,Pérez-Arellano JL,Carranza C,Puente S,López-Abán J,Muro Adoi
10.1017/S0031182006000898subject
Has Abstractpub_date
2006-11-01 00:00:00pages
581-7issue
Pt 5eissn
0031-1820issn
1469-8161pii
S0031182006000898journal_volume
133pub_type
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