Enzyme variation in T. brucei ssp. II. Evidence for T. b. rhodesiense being a set of variants of T. b. brucei.

Abstract:

:A collection of stocks of Trypanosoma brucei rhodesiense isolated in Kenya have been examined for electrophoretic variation in 20 enzymes. The results obtained have been analysed in order to determine whether these trypanosomes are diploid and undergo mating and to determine the genetic distance between T. b. rhodesiense, T. b. brucei and T. b. gambiense. The enzyme electrophoretic markers were further used in experiments involving cyclically transmitted mixtures of stocks aimed at detecting genetic exchange in the laboratory. No genetic exchange was detected. Two novel features of the enzyme electrophoretic results were found. Firstly, the stocks of T. b. rhodesiense were considerably more homogeneous than equivalent collections of stocks of T. b. brucei and secondly, all the stocks examined were heterozygous for two alleles of alkaline phosphatase and showed an excess of heterozygotes at the phosphoglucomutase locus. The degree to which these features are typical of T. b. rhodesiense has been examined in relation to previously published data. The results obtained strongly support the view that T. b. rhodesiense is a set of variants of T. b. brucei rather than a subspecies and a working hypothesis as to the relationship between T. b. brucei and T. b. rhodesiense is proposed to explain the enzyme electrophoretic data obtained.

journal_name

Parasitology

journal_title

Parasitology

authors

Tait A,Barry JD,Wink R,Sanderson A,Crowe JS

doi

10.1017/s0031182000049040

subject

Has Abstract

pub_date

1985-02-01 00:00:00

pages

89-100

eissn

0031-1820

issn

1469-8161

journal_volume

90 ( Pt 1)

pub_type

杂志文章
  • Altered lysophospholipase B responsiveness in lactating mice infected with intestinal nematode parasites.

    abstract::Lactating and nulliparous outbred Swiss (CF-1 strain) mice were infected at 12-16 weeks of age with Nippostrongylus brasiliensis or Trichinella spiralis. Lysophospholipase B levels in the intestinal tissue and faecal pellets were greatly in infected lactating mice in contrast to infected nulliparous mice. Correlated w...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000054998

    authors: Ngwenya BZ

    更新日期:1980-08-01 00:00:00

  • Sub-cellular fractionation of Trypanosoma brucei. Isolation and characterization of plasma membranes.

    abstract::A procedure is described for the isolation of sub-cellular fractions from bloodstream forms of Trypanosoma brucei. The method leaves intact most of the nuclei, mitochondria and microbodies. All the fractions have been chemically characterized and tested for 10 enzymatic markers. About 5% of total cell protein was isol...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000000974

    authors: Rovis L,Baekkeskov S

    更新日期:1980-06-01 00:00:00

  • Four new species of KudoaMeglitsch, 1947 (Myxosporea: Multivalvulida) from Australia with recommendations for species descriptions in the Kudoidae.

    abstract::Kudoid parasites are significant pathogens of marine fish. In the past, specific identification has been difficult due to a paucity of detailed morphological and biological information provided in the original description of some species. However, the introduction of DNA analysis has dramatically improved diagnosis. F...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009991557

    authors: Burger MA,Adlard RD

    更新日期:2010-04-01 00:00:00

  • Five types of nuclei present in the capsule of Trichinella spiralis.

    abstract::This study describes morphological changes during capsule formation of Trichinella spiralis with emphasis on nuclei within the capsule. The nuclei of the pre-existing muscle cell may become hypertrophied or degrade soon after the entrance of newborn larvae. Cytoplasm associated with these hypertrophied nuclei was baso...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099006198

    authors: Matsuo A,Wu Z,Nagano I,Takahashi Y

    更新日期:2000-08-01 00:00:00

  • Evaluation of salinomycin isolated from Streptomyces albus JSY-2 against the ciliate, Ichthyophthirius multifiliis.

    abstract::The present study was undertaken to investigate the antiparasitic activity of extracellular products of Streptomyces albus. Bioactivity-guided isolation of chloroform extracts affording a compound showing potent activity. The structure of the compound was elucidated as salinomycin (SAL) by EI-MS, 1H NMR and 13C NMR. I...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182018001919

    authors: Yao JY,Gao MY,Jia YY,Wu YX,Yin WL,Cao Z,Yang GL,Huang HB,Wang CF,Shen JY,Gu ZM

    更新日期:2019-04-01 00:00:00

  • Cytochemical studies on nuclear DNA of four eucoccidian parasites, Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei.

    abstract::Feulgen-pararosaniline (SO2) staining was performed on stages in the life-cycle of Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei. The fluorescence emission of the stained DNA in nuclei of these stages was examined and compared with absorption microscopy measurements at 560 nm ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000054305

    authors: Cornelissen AW,Overdulve JP,Van der Ploeg M

    更新日期:1984-02-01 00:00:00

  • A novel duplex ddPCR assay for the diagnosis of schistosomiasis japonica: proof of concept in an experimental mouse model.

    abstract::The current World Health Organization strategic plan targets the elimination of schistosomiasis as a public health problem by 2025 and accurate diagnostics will play a pivotal role in achieving this goal. DNA-based detection methods provide a viable alternative to some of the commonly used tests, notably microscopy an...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201700021X

    authors: Weerakoon KG,Gordon CA,Cai P,Gobert GN,Duke M,Williams GM,McManus DP

    更新日期:2017-07-01 00:00:00

  • Mathematical models of parasite responses to host immune defences.

    abstract::We examine the evolution of microparasites in response to the immune system of vertebrate hosts. We first describe a simple model for an acute infection. This model suggests that the within-host dynamics of the microparasite will be a 'race' between parasite multiplication and a clonally expanding response by the host...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s003118209700200x

    authors: Antia R,Lipsitch M

    更新日期:1997-01-01 00:00:00

  • Schistosoma mansoni: changes in elemental composition in relation to the age and sexual status of the worms.

    abstract::Changes during development in the elemental contents (Ca, P, K, Mg and S) of male and female Schistosoma mansoni from both mixed and single sex infections have been measured for the first time. In mixed infections, there is a significant difference between the calcium content of male and female worms; female worms hav...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000050630

    authors: Shaw MK,Erasmus DA

    更新日期:1983-06-01 00:00:00

  • Asparaginyl endopeptidase activity in adult Schistosoma mansoni.

    abstract::Sequence comparisons have recently shown that the Schistosoma mansoni protein Sm32 is similar to asparaginyl endoproteinases, a novel family of cysteine proteinases, of which the legumains from legumes are the best characterized. By synthesizing and employing fluorogenic peptide substrates for the specific detection o...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000077052

    authors: Dalton JP,Hola-Jamriska L,Brindley PJ

    更新日期:1995-12-01 00:00:00

  • Density-dependent processes in the transmission of human onchocerciasis: intensity of microfilariae in the skin and their uptake by the simuliid host.

    abstract::The transmission success of Onchocerca volvulus is thought to be influenced by a variety of regulatory or density-dependent processes that act at various points in the two-host life-cycle. This paper examines one component of the life-cycle, namely, the ingestion of microfilariae by the simuliid vector, to assess the ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000078586

    authors: Basáñez MG,Boussinesq M,Prod'hon J,Frontado H,Villamizar NJ,Medley GF,Anderson RM

    更新日期:1994-01-01 00:00:00

  • The parasitism of Schistosoma mansoni (Digenea-Trematoda) in a naturally infected population of water rats, Nectomys squamipes (Rodentia-Sigmodontinae) in Brazil.

    abstract::Schistosomiasis is a health problem in Brazil and the role of rodents in maintaining the schistosome life-cycle requires further clarification. The influence of Schistosoma mansoni on a population of Nectomys squamipes was studied by capture-recapture (1st phase, from June 1991 to November 1995) and removal (2nd phase...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005892

    authors: D'Andrea PS,Maroja LS,Gentile R,Cerqueira R,Maldonado Júnior A,Rey L

    更新日期:2000-06-01 00:00:00

  • An investigation of fructose utilization in Acanthocheilonema viteae.

    abstract::The capacity of Acanthocheilonema viteae to metabolize fructose was investigated in vitro. In common with other filarial species A. viteae oxidized fructose to lactate but its rate of consumption was only 40% of the glucose-containing control value. Fructose was not incorporated into glycogen. Release of 14CO2 from [U...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000060649

    authors: Hayes DJ,Carter NS

    更新日期:1990-12-01 00:00:00

  • Widespread infection of the Eastern red-spotted newt (Notophthalmus viridescens) by a new species of Amphibiocystidium, a genus of fungus-like mesomycetozoan parasites not previously reported in North America.

    abstract::Given the worldwide decline of amphibian populations due to emerging infectious diseases, it is imperative that we identify and address the causative agents. Many of the pathogens recently implicated in amphibian mortality and morbidity have been fungal or members of a poorly understood group of fungus-like protists, ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182007003708

    authors: Raffel TR,Bommarito T,Barry DS,Witiak SM,Shackelton LA

    更新日期:2008-02-01 00:00:00

  • High polymorphism in Plasmodium vivax merozoite surface protein-5 (MSP5).

    abstract::A key issue relating to developing multi-component anti-malarial vaccines, lies in studying Plasmodium vivax surface proteins' genetic variation. The present work was aimed at amplifying, cloning and sequencing the gene encoding P. vivax merozoite surface protein 5 (PvMSP5) in samples obtained from infected patients f...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006001168

    authors: Gomez A,Suarez CF,Martinez P,Saravia C,Patarroyo MA

    更新日期:2006-12-01 00:00:00

  • Field evidence for density-dependent facilitation amongst Ixodes ricinus ticks feeding on sheep.

    abstract::Engorged Ixodes ricinus nymphs collected from sheep resident in an upland UK field site were significantly lighter than nymphs that engorged on previously tick-naïve sheep, indicating that site-resident sheep continually exposed to ticks acquired anti-tick resistance. The weights of engorged nymphs that fed on natural...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001001081

    authors: Ogden NH,Casey AN,French NP,Adams JD,Woldehiwet Z

    更新日期:2002-02-01 00:00:00

  • The epidemiology of canine leishmaniasis: transmission rates estimated from a cohort study in Amazonian Brazil.

    abstract::We estimate the incidence rate, serological conversion rate and basic case reproduction number (R0) of Leishmania infantum from a cohort study of 126 domestic dogs exposed to natural infection rates over 2 years on Marajó Island, Pará State, Brazil. The analysis includes new methods for (1) determining the number of s...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182097001200

    authors: Quinnell RJ,Courtenay O,Garcez L,Dye C

    更新日期:1997-08-01 00:00:00

  • What helminth genomes have taught us about parasite evolution.

    abstract::The genomes of more than 20 helminths have now been sequenced. Here we perform a meta-analysis of all sequenced genomes of nematodes and Platyhelminthes, and attempt to address the question of what are the defining characteristics of helminth genomes. We find that parasitic worms lack systems for surface antigenic var...

    journal_title:Parasitology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1017/S0031182014001449

    authors: Zarowiecki M,Berriman M

    更新日期:2015-02-01 00:00:00

  • Purification of an agglutinin from the haemolymph of the snail Bulinus nasutus and demonstration of related proteins in other Bulinus spp.

    abstract::The snail Bulinus nasutus 1214 possesses a potent haemagglutinin (end-point titre with human erythrocytes, 2(-18)) in its cell-free haemolymph which also binds to the miracidia (but not other larvae) of the incompatible parasite Schistosoma margrebowiei. We have purified a protein possessing this haemagglutinating pro...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000074928

    authors: Harris RA,Preston TM,Southgate VR

    更新日期:1993-02-01 00:00:00

  • Resistance to Onchocerca lienalis microfilariae in mice conferred by egg antigens of homologous and heterologous Onchocerca species.

    abstract::Embryonic stages of various Onchocerca species have been used to stimulate resistance in CBA mice to challenge injections with the microfilariae of Onchocerca lienalis. Comparable levels of resistance to challenge (29-37% reductions) were conferred by living, freeze-killed, or sonicated organisms administered with Fre...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055839

    authors: Carlow CK,Bianco AE

    更新日期:1987-06-01 00:00:00

  • The molecular systematics of blowflies and screwworm flies (Diptera: Calliphoridae) using 28S rRNA, COX1 and EF-1α: insights into the evolution of dipteran parasitism.

    abstract::The Calliphoridae include some of the most economically significant myiasis-causing flies in the world - blowflies and screwworm flies - with many being notorious for their parasitism of livestock. However, despite more than 50 years of research, key taxonomic relationships within the family remain unresolved. This st...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011001089

    authors: McDonagh LM,Stevens JR

    更新日期:2011-11-01 00:00:00

  • Tracking a heterosis effect in the field: tadpole resistance to parasites in the water frog hybridogenetic complex.

    abstract::Depending on the extent of evolutionary divergence among parent taxa, hybrids may suffer from a breakdown of co-adapted genes or may conversely exhibit vigour due to the heterosis effect, which confers advantages to increased genetic diversity. That last mechanism could explain the success of hybrids when hybridizatio...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009006489

    authors: Planade B,Lena JP,Li H,Plenet S,Guegan JF,Thomas F,Hurtrez-Bousses S,Renaud F,Joly P

    更新日期:2009-08-01 00:00:00

  • A panel of antigens of muscle larvae of Trichinella spiralis and T. pseudospiralis as revealed by two-dimensional western blot and immunoelectron microscopy.

    abstract::This study characterized antigens of Trichinella spiralis and T. pseudospiralis muscle larvae recognized by mice infected with the worms. Two-dimensional (2-D) Western blot analysis revealed some profile of antigenic peptides including: (1) molecular weight (MW); (2) isoelectric points (pI), (3) reactivity to well-def...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099004187

    authors: Wu Z,Nagano I,Takahashi Y

    更新日期:1999-06-01 00:00:00

  • Schistosoma mansoni from naturally infected Rattus rattus in Guadeloupe: identification, prevalence and enzyme polymorphism.

    abstract::An enzymatic comparison has been made between isolates of Schistosoma mansoni from rats and humans in Guadeloupe and a Burundi isolate of S. rodhaini. Analyses of LDH, MDH, AcP, PGM, GPI, G6PDH and HK by isoelectric focusing provided no evidence for the involvement of S. rodhaini in the recent evolution of the schisto...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000049817

    authors: Rollinson D,Imbert-Establet D,Ross GC

    更新日期:1986-08-01 00:00:00

  • Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system.

    abstract::Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009990837

    authors: Borowski H,Thompson RC,Armstrong T,Clode PL

    更新日期:2010-01-01 00:00:00

  • A comparative analysis of various developmental stages of Schistosoma mansoni with respect to their protein composition.

    abstract::The protein composition of Schistosoma mansoni was analysed by electrophoresis in polyacrylamide slab gel in the presence of sodium dodecyl sulphate. A high degree of identity existed between protein patterns obtain from immature and adult parasites of the two sexes. Some stage-and sex-specific components were indenti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118200005188x

    authors: Ruppel A,Cioli D

    更新日期:1977-12-01 00:00:00

  • Protein synthesis and release by cultured schistosomula of Schistosoma mansoni.

    abstract::The lung schistosomulum of Schistosoma mansoni is the target of protective immunity in mice singly vaccinated with irradiated cercariae. Since the effector responses are T cell-mediated, their initiation requires the release of antigens from the intact parasite. We have used the technique of biosynthetic labelling wit...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000079245

    authors: Harrop R,Wilson RA

    更新日期:1993-09-01 00:00:00

  • Dual congenital transmission of Toxoplasma gondii and Sarcocystis neurona in a late-term aborted pup from a chronically infected southern sea otter (Enhydra lutris nereis).

    abstract::Toxoplasma gondii and Sarcocystis neurona are protozoan parasites with terrestrial definitive hosts, and both pathogens can cause fatal disease in a wide range of marine animals. Close monitoring of threatened southern sea otters (Enhydra lutris nereis) in California allowed for the diagnosis of dual transplacental tr...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182015001377

    authors: Shapiro K,Miller MA,Packham AE,Aguilar B,Conrad PA,Vanwormer E,Murray MJ

    更新日期:2016-03-01 00:00:00

  • Guinea worm: from Robert Leiper to eradication.

    abstract::Guinea worm disease, dracunculiasis or dracontiasis, is an ancient disease with records going back over 4500 years, but until the beginning of the 20th century, little was known about its life cycle, particularly how humans became infected. In 1905, Robert Thomas Leiper was sent by the British colonial authorities to ...

    journal_title:Parasitology

    pub_type: 传,历史文章,杂志文章,评审

    doi:10.1017/S0031182017000683

    authors: Tayeh A,Cairncross S,Cox FEG

    更新日期:2017-10-01 00:00:00

  • RNA-interference methods for gene-knockdown in the sea louse, Lepeophtheirus salmonis: studies on a putative prostaglandin E synthase.

    abstract::Harnessing the full utility of extensive gene sequences recently available for the economically important sea louse, Lepeophtheirus salmonis, requires the adaptation of modern molecular biology approaches to this non-model organism. Using a putative microsomal prostaglandin E synthase type-2 (PGES2) as a candidate gen...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009990357

    authors: Campbell EM,Pert CC,Bowman AS

    更新日期:2009-07-01 00:00:00