The effect of drugs on the cell cycle of Giardia intestinalis.

Abstract:

:Flow cytometric analysis of the binucleated protozoan parasite Giardia intestinalis gave DNA histograms with a broad G1 peak and a definable G2 + M peak with twice the DNA content of G1. Twenty-four hour treatment with metronidazole arrested cell cycle progression of susceptible trophozoites in the G2 + M phase, but had no effect, even at toxic doses, on the DNA histogram of a line selected for resistance to metronidazole. Furazolidone was inhibitory to both stocks, causing an arrest in the S and G2 + M phases. Inhibitors of the mammalian cell cycle were also tested. Hydroxyurea, which blocks mammalian cells in G1/S, and razoxane, which blocks in G2 + M, arrested trophozoites in the G2 + M phase whereas colchicine and gamma-irradiation had little or no effect on the cell cycle of G. intestinalis. These results suggest that the cell cycle of G. intestinalis may be controlled in a different manner from mammalian cells.

journal_name

Parasitology

journal_title

Parasitology

authors

Hoyne GF,Boreham PF,Parsons PG,Ward C,Biggs B

doi

10.1017/s0031182000059047

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

333-9

eissn

0031-1820

issn

1469-8161

journal_volume

99 Pt 3

pub_type

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