Abstract:
:Toxoplasma gondii is an obligate intracellular protozoan parasite. The objective of the present study was to examine the behaviour of Toxoplasma gondii RH Ankara strain tachyzoites in a cell culture environment. The study represents the first step in determining whether T. gondii RH Ankara strain tachyzoites, grown in cell culture, are of sufficient quality to allow cessation of in vivo tachyzoite production for diagnostic assays. In the present study, T. gondii RH Ankara strain tachyzoites were continuously produced in myeloma X63.Ag8.653, HeLa, Hep-2, and Vero cell cultures for 2 months. The average size of the tachyzoites was 3 x 5.7 microm prior to the first inoculation but after continuous production, a marked decrease was noted in average tachyzoite size. The smallest tachyzoite size, was 1 x 2.1 microm after 2 months, in myeloma cell cultures even though the yield of tachyzoites increased. With other cell cultures, tachyzoite yields were not as high as myeloma cell culture although decrease in size was less. The smallest decrease in tachyzoite size, averaging 2 x 3.8 microm after 2 months, was observed in tachyzoites produced in HeLa cell cultures. A virulence assay in small groups of BALB/c mice, using tachyzoites derived from cell cultures, was also conducted. The preliminary results of the virulence assay suggest that as the size of the tachyzoites decreased, the virulence in mice decreased. Future research will focus on the effect of the size of cell culture-derived T. gondii RH Ankara strain tachyzoites on the virulence, protein expression, and the reliability of diagnostic assays. Ultimately, the behaviour of tachyzoites from various T. gondii strains will be observed in cell culture to determine if size is altered.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Döskaya M,Degirmenci A,Ciçek C,Ak M,Korkmaz M,Gürüz Y,Uner Adoi
10.1017/S0031182005009078subject
Has Abstractpub_date
2006-03-01 00:00:00pages
315-9issue
Pt 3eissn
0031-1820issn
1469-8161pii
S0031182005009078journal_volume
132pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::The ultrastructure of the seminal vesicle, ejaculatory duct, cirrus sac and cirrus is described. The epithelium of the seminal vesicle consists of a single layer of squamous to cuboidal cells. The apical ends of the cells have thin polymorphic lamellae and long narrow pits, both of which enclose normal spermatozoa. Th...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200004720x
更新日期:1975-12-01 00:00:00
abstract::Diseases caused by viruses are a constant and major problem for livestock production world wide. The diseases range from highly contagious acute forms with high mortality, to chronic disabling diseases with an insidious effect on production. Such diseases cannot be regarded as static in nature due to the highly mutabl...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182099004199
更新日期:1998-01-01 00:00:00
abstract::A double antibody sandwich ELISA technique has been developed for detection of antigens of African trypanosomes present in the sera of infected mammals. The assay uses a high titre, high affinity rabbit antiserum made to purified membranes of procyclic trypanosomes as "capture" reagent and a mixture of three biotin-la...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000057735
更新日期:1987-10-01 00:00:00
abstract::Australian tea tree oil (TTO) and its extract terpinen-4-ol (T4O) are found to be effective in moderating demodex-related diseases. Their possible effects are lowering the mite counts, relieving the demodex-related symptoms and modulating the immune system especially the inflammatory response. This review summarizes t...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182018000495
更新日期:2018-10-01 00:00:00
abstract::A guinea-pig immunoglobulin-G linked agarose column was used to detect the immunoglobulin-G binding proteins (IGBP) in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus. IGBPs were found in salivary gland extract (SGE) of the unfed ticks of all 3 species. The sizes of the IGBPs,...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000064908
更新日期:1995-08-01 00:00:00
abstract::The epidemiological and ecological processes which govern the success of multiple-species co-infections are as yet unresolved. Here we investigated prior versus late residency within hosts, meaning which parasite contacts the host first, to determine if the outcomes of intra-host competition are altered. We infected a...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182010000296
更新日期:2010-09-01 00:00:00
abstract::Cercariae of the bird schistosome Trichobilharzia regenti and of the human schistosome Schistosoma mansoni employ proteases to invade the skin of their definitive hosts. To investigate whether a similar proteolytic mechanism is used by both species, cercarial extracts of T. regenti and S. mansoni were biochemically ch...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182007002910
更新日期:2007-10-01 00:00:00
abstract::Infectious agents have considerable potential to regulate or constrain the population growth of vertebrate hosts in natural habitats. A broad theoretical framework provides many insights into how the biology of the parasite and the demography of the host interact to determine this impact. It may manifest itself as a s...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s003118200007579x
更新日期:1995-01-01 00:00:00
abstract::In this study, the proteome of axenically grown Entamoeba histolytica parasites was explored by two-dimensional gel electrophoresis (2-DE), employing a practical and effective procedure for the solubilization of E. histolytica proteins. Approximately 900 protein species in the pH range between 4 and 7 were detected by...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006001442
更新日期:2007-02-01 00:00:00
abstract::West-central China is an important endemic focus of both alveolar and cystic echinococcosis where several species of intermediate host are commonly infected with Echinococcus granulosus and E. multilocularis . Isolates of E. granulosus were collected from humans and other animals from different geographical areas of Q...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005007973
更新日期:2005-10-01 00:00:00
abstract::Susceptibility to oxidative stress is a well-established feature of the malarial parasite. Pharmacologists have taken advantage of this property to design highly effective pro-oxidant antimalarial drugs. Less well appreciated is the fact that nutritional manipulation of host oxidative stress status by dietary means ca...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000075533
更新日期:1993-01-01 00:00:00
abstract::Accurately identifying resistance to gastrointestinal nematode infections requires the ability to identify animals with low and high intensities of infection. The pathogenic effects of nematodes depend upon both the length and number of worms, neither of which can be measured in live animals. Indices that predict thes...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182014001905
更新日期:2015-05-01 00:00:00
abstract::Genome assemblies can form the basis of comparative analyses fostering insight into the evolutionary genetics of a parasite's pathogenicity, host-pathogen interactions, environmental constraints and invasion biology; however, the length and complexity of many parasite genomes has hampered the development of well-resol...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017000348
更新日期:2017-09-01 00:00:00
abstract::Nitric oxide (NO) produced by cytokine-activated macrophages is reported to be cytotoxic against the helminth Schistosoma mansoni, although this is a controversial issue. Previous work in our laboratory identified a fraction of S. mansoni soluble adult worm antigenic preparation (SWAP), named PIII, able to elicit sign...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099005636
更新日期:2000-04-01 00:00:00
abstract::Here, the validity of the assumption of concerted evolution of ribosomal regions in larval and adult Cooperia oncophora was assessed. In each of 4 individuals of this parasitic nematode, at least 78% of the sequences comprised different ITS variants. This implies that concerted evolution is not acting, which is corrob...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004005499
更新日期:2004-08-01 00:00:00
abstract::Blastocystis SSU-rDNA sequence data from 317 captive and free-living non-human primates (NHPs) representing 30 genera of apes, Old and New World (OW and NW) monkeys and prosimians were analysed to investigate subtype (ST) and allele distribution among hosts. Excluding 20 mixed ST infections, 27% of the sequences belon...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013000255
更新日期:2013-07-01 00:00:00
abstract::Recent studies of the forces behind the diversification of parasite assemblages have shed light on many aspects of parasite biodiversity. By using only parasite species richness as their measure of diversity, however, previous investigations have ignored the relatedness among parasite species and the taxonomic structu...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004005050
更新日期:2004-06-01 00:00:00
abstract::Hymenolepis microstoma from secondary infections in mice were found to grow initially more slowly than H. microstoma from primary infections. Eventually a similar rate of growth was attained by both kinds of worm. After transplantation, young worms (less than or equal to 4 days old) grew more slowly in previously infe...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000062387
更新日期:1977-10-01 00:00:00
abstract::The diversity and prevalence of malaria parasites of the genera Plasmodium and Haemoproteus were determined in the globally-threatened Aquatic Warbler Acrocephalus paludicola. Birds were sampled during migration in Portugal and at the wintering quarters in Senegal and parasites were detected using molecular methods. O...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015000414
更新日期:2015-08-01 00:00:00
abstract::Risk factors for the transmission of Echinococcus multilocularis to humans operate at a range of spatial scales. Over a large area, such as China, regional scale risk is correlated with variation in climatic conditions because of its effect on the spatial distribution of landscapes that can support E. multilocularis t...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00
abstract::A key issue relating to developing multi-component anti-malarial vaccines, lies in studying Plasmodium vivax surface proteins' genetic variation. The present work was aimed at amplifying, cloning and sequencing the gene encoding P. vivax merozoite surface protein 5 (PvMSP5) in samples obtained from infected patients f...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006001168
更新日期:2006-12-01 00:00:00
abstract::The protein composition of Schistosoma mansoni was analysed by electrophoresis in polyacrylamide slab gel in the presence of sodium dodecyl sulphate. A high degree of identity existed between protein patterns obtain from immature and adult parasites of the two sexes. Some stage-and sex-specific components were indenti...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200005188x
更新日期:1977-12-01 00:00:00
abstract::A metabolic pathway known as the mannitol cycle has been identified in Eimerian parasites. The pathway is a shunt off of the glycolytic pathway at fructose-6-phosphate (F6P). Two enzymes convert F6P to mannitol and two other enzymes are responsible for converting mannitol back to F6P when it is utilized. Although the ...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
abstract::Susceptability of Ascaridia galli to benzimidazole (BZ) was investigated using faecal egg count reduction test (FECRT), in ovo larval development test (LDT) and genetic markers (mutations at codons 167, 198 and 200 of β-tubulin gene). Six flocks (F1-F6) of a commercial laying hen farm with different number of exposure...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017000531
更新日期:2017-09-01 00:00:00
abstract::Cystic echinococcosis (CE) is a high prevalence zoonosis among the rural population of Tacuarembó (Uruguay). The correlation between serological data and the incidence of risk factors was studied in a survey carried out in 1998 among rural communities where 480 individuals were examined by means of abdominal sonograph...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006717
更新日期:2005-04-01 00:00:00
abstract::Stability of trichostrogylid populations indicates that some form of density-dependent regulation occurs which could act through fecundity. We present evidence for intraspecific density-dependent effects in 1 of 2, dominant, abomasal nematodes species (Ostertagia gruehneri) of Svalbard reindeer (Rangifer tarandus plat...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001007818
更新日期:2001-06-01 00:00:00
abstract::Most sylvatic species of Trichinella are known to have poor infectivity to rats, but in the present study oral administration of bile from other hosts appeared to modify this infectivity. A total of 75 rats were inoculated in groups of 25 with 3 species of Trichinella (T. spiralis, T. nativa, and T. nelsoni) and each ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200300307x
更新日期:2003-05-01 00:00:00
abstract::Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet ge...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000061679
更新日期:1992-04-01 00:00:00
abstract::Ivermectin treatment may induce severe adverse reactions in some individuals heavily infected with Loa loa. This hampers the implementation of mass ivermectin treatment against onchocerciasis in areas where Onchocerca volvulus and L. loa are co-endemic. In order to identify factors, including co-infections, which may ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118200600984X
更新日期:2006-06-01 00:00:00
abstract::The host recognition and invasion process of Myxobolus cerebralis actinospores (triactinomyxon, TAM) was studied on a genetic level. A small-scale in vitro assay was developed to activate a large number of TAMs simultaneously, and to monitor the host invasion in the absence of live fish. The transcriptomes of non-acti...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008005398
更新日期:2009-04-01 00:00:00