Trypanosoma cruzi heparin-binding proteins present a flagellar membrane localization and serine proteinase activity.

Abstract:

:Heparin-binding proteins (HBPs) play a key role in Trypanosoma cruzi-host cell interactions. HBPs recognize heparan sulfate (HS) at the host cell surface and are able to induce the cytoadherence and invasion of this parasite. Herein, we analysed the biochemical properties of the HBPs and also evaluated the expression and subcellular localization of HBPs in T. cruzi trypomastigotes. A flow cytometry analysis revealed that HBPs are highly expressed at the surface of trypomastigotes, and their peculiar localization mainly at the flagellar membrane, which is known as an important signalling domain, may enhance their binding to HS and elicit the parasite invasion. The plasmon surface resonance results demonstrated the stability of HBPs and their affinity to HS and heparin. Additionally, gelatinolytic activities of 70 kDa, 65·8 kDa and 59 kDa HBPs over a broad pH range (5·5-8·0) were revealed using a zymography assay. These proteolytic activities were sensitive to serine proteinase inhibitors, such as aprotinin and phenylmethylsulfonyl fluoride, suggesting that HBPs have the properties of trypsin-like proteinases.

journal_name

Parasitology

journal_title

Parasitology

authors

Oliveira FO Jr,Alves CR,Silva FS,Côrtes LM,Toma L,Bouças RI,Aguilar T,Nader HB,Pereira MC

doi

10.1017/S0031182012001448

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

171-80

issue

2

eissn

0031-1820

issn

1469-8161

pii

S0031182012001448

journal_volume

140

pub_type

杂志文章
  • The specificity of purine base and nucleoside uptake in promastigotes of Leishmania braziliensis panamensis.

    abstract::Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported a...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055256

    authors: Hansen BD,Perez-Arbelo J,Walkony JF,Hendricks LD

    更新日期:1982-10-01 00:00:00

  • Immunologically mediated, non-specific interactions between the intestinal phases of Trichinella spiralis and Nippostrongylus brasiliensis in the mouse.

    abstract::Interactions between infections of Trichinella spiralis and Nippostrongylus brasiliensis were studied in the NIH strain of mouse which is known to react strongly to T. spiralis. The course of N. brasiliensis infection in this strain of mouse is described and expulsion is shown to be accelerated in immunized mice and i...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000000512

    authors: Kennedy MW

    更新日期:1980-02-01 00:00:00

  • Conventional PCR for molecular diagnosis of human strongyloidiasis.

    abstract::Strongyloidiasis is frequently asymptomatic and diagnosis of latent infection is difficult due to limitations of current parasitological and serological methods. This study aimed to verify the use of conventional polymerase chain reaction (PCR) assay for molecular diagnosis of Strongyloides stercoralis infection. Fres...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182013002035

    authors: Sitta RB,Malta FM,Pinho JR,Chieffi PP,Gryschek RC,Paula FM

    更新日期:2014-04-01 00:00:00

  • Aggravation of pathogenesis mediated by ochratoxin A in mice infected with Trypanosoma brucei rhodesiense.

    abstract::Mice fed 1.5 mg ochratoxin A (OTA) per kg body weight and infected with Trypanosoma brucei rhodesiense were compared with trypanosome-infected placebo-fed and uninfected OTA-fed controls. Uninfected OTA-fed mice showed fever, lethargy, facial and eyelid oedemas, mild hepatitis and nephritis, and high survival. Infecte...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008005386

    authors: Kibugu JK,Ngeranwa JJ,Makumi JN,Gathumbi JK,Kagira JM,Mwangi JN,Muchiri MW,Mdachi RE

    更新日期:2009-03-01 00:00:00

  • Characterization of the mitochondrial genome of Diphyllobothrium latum (Cestoda: Pseudophyllidea) - implications for the phylogeny of eucestodes.

    abstract::The complete nucleotide sequence of the mitochondrial genome was determined for the fish tapeworm Diphyllobothrium latum. This genome is 13,608 bp in length and encodes 12 protein-coding genes (but lacks the atp8), 22 transfer RNA (tRNA) and 2 ribosomal RNA (rRNA) genes, corresponding to the gene complement found thus...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118200600206X

    authors: Park JK,Kim KH,Kang S,Jeon HK,Kim JH,Littlewood DT,Eom KS

    更新日期:2007-05-01 00:00:00

  • Neoglycosylated liposomes as efficient ligands for the evaluation of specific sugar receptors on macrophages in health and in experimental leishmaniasis.

    abstract::Receptors interacting with terminal sugars as ligands are involved in the binding of Leishmania donovani promastigotes to the macrophage surface and their subsequent internalization. Mannose and glucose are specifically involved in the binding process. Decreased binding occurs to macrophages already infected with L. d...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000076253

    authors: Dutta M,Bandyopadhyay R,Basu MK

    更新日期:1994-08-01 00:00:00

  • Pine chemical volatiles promote dauer recovery of a pine parasitic nematode, Bursaphelenchus xylophilus.

    abstract::Pinewood nematode, Bursaphelenchus xylophilus, a pine parasitic nematode, poses a serious threat to its host pine forests globally. When dispersal-stage larvae 4 (dauer, DL4) of B. xylophilus enters the new pine, it moults into propagative adult (dauer recovery) and reproduces quickly to kill the host pine. Here, we f...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182019001264

    authors: Zhang W,Li Y,Pan L,Wang X,Feng Y,Zhang X

    更新日期:2020-01-01 00:00:00

  • Towards a mechanistic understanding of competence: a missing link in diversity-disease research.

    abstract::Biodiversity loss may increase the risk of infectious disease in a phenomenon known as the dilution effect. Circumstances that increase the likelihood of disease dilution are: (i) when hosts vary in their competence, and (ii) when communities disassemble predictably, such that the least competent hosts are the most li...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020000943

    authors: Stewart Merrill TE,Johnson PTJ

    更新日期:2020-09-01 00:00:00

  • RNA-interference methods for gene-knockdown in the sea louse, Lepeophtheirus salmonis: studies on a putative prostaglandin E synthase.

    abstract::Harnessing the full utility of extensive gene sequences recently available for the economically important sea louse, Lepeophtheirus salmonis, requires the adaptation of modern molecular biology approaches to this non-model organism. Using a putative microsomal prostaglandin E synthase type-2 (PGES2) as a candidate gen...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009990357

    authors: Campbell EM,Pert CC,Bowman AS

    更新日期:2009-07-01 00:00:00

  • Inside Doctor Livingstone: a Scottish icon's encounter with tropical disease.

    abstract::Dr David Livingstone died on May 1st 1873. He was 60 years old and had spent much of the previous 30 years walking across large stretches of Southern Africa, exploring the terrain he hoped could provide new environments in which Europeans and Africans could cohabit on equal terms and bring prosperity to a part of the ...

    journal_title:Parasitology

    pub_type: 传,历史文章,杂志文章

    doi:10.1017/S003118201600202X

    authors: Barrett MP,Giordani F

    更新日期:2017-10-01 00:00:00

  • Activity of quinfamide against natural infections of Entamoeba criceti in hamsters: a new potent agent for intestinal amoebiasis.

    abstract::A novel tetrahydroquinolinyl ester, quinfamide, administered orally in multiple doses for 3 days had an ED50 of 0.25 mg/kg/day (total dose 0.75 mg/kg) for eradicating Entamoeba criceti in hamsters in several tests. It was significantly more active by direct comparison than 3 commercially available amoebicides and at l...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055128

    authors: Slighter RG,Yarinsky A,Drobeck HP,Bailey DM

    更新日期:1980-08-01 00:00:00

  • Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out-bred, in-bred) on pathogenesis and mortality.

    abstract::Humans and other hosts acquire Toxoplasma gondii infection by ingesting tissue cysts in undercooked meat, or by food or drink contaminated with oocysts. Currently, there is no vaccine to prevent clinical disease due this parasite in humans, although, various T. gondii vaccine candidates are being developed. Mice are g...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011001673

    authors: Dubey JP,Ferreira LR,Martins J,McLeod R

    更新日期:2012-01-01 00:00:00

  • The larvicidal activity of cyclosporin A against Schistosoma mansoni in mice.

    abstract::Treatment of BALB/c or MF1 mice with cyclosporin A (CsA) around the time of infection with Schistosoma mansoni conferred almost complete protection. The migration kinetics of L-[75Se]selenomethionine-labelled infective cercariae were investigated by compressed tissue autoradiography. Similar levels of skin penetration...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000060340

    authors: Munro GH,Brannan LR,Chappell LH,Thomson AW,McLaren DJ

    更新日期:1991-02-01 00:00:00

  • Comparison of the transmissibility of Trypanosoma congolense strains, isolated in a trypanosomiasis endemic area of eastern Zambia, by Glossina morsitans morsitans.

    abstract::Transmission experiments were conducted to compare the transmissibility of genetically different Trypanosoma congolense (Savannah subgroup) strains isolated from cattle in a trypanosomiasis endemic area of eastern Zambia. A total of 17 strains were compared. Three strains were extremely virulent with a short pre-paten...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006000369

    authors: Masumu J,Marcotty T,Ndeledje N,Kubi C,Geerts S,Vercruysse J,Dorny P,van den Bossche P

    更新日期:2006-09-01 00:00:00

  • The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst.

    abstract::Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular laye...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006000400

    authors: Bahia D,Avelar LG,Vigorosi F,Cioli D,Oliveira GC,Mortara RA

    更新日期:2006-09-01 00:00:00

  • Fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae of Ascaris suum.

    abstract::Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process of Ascaris suum larvae. This study describes moulting and development-specific protein expression profiles of A. suum lung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepa...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006000576

    authors: Islam MK,Miyoshi T,Yamada M,Alim MA,Huang X,Motobu M,Tsuji N

    更新日期:2006-10-01 00:00:00

  • Mimotope-based antigens as potential vaccine candidates in experimental murine cysticercosis.

    abstract::Human cysticercosis is a public health problem caused by Taenia solium metacestodes; thus, eradication of T. solium transmission by vaccination is an urgent requirement. The Cc48 mimotope from T. solium cysticerci was tested expressed in phage particles (mCc48) and chemically synthesized (sCc48) as a vaccine candidate...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020001080

    authors: Manhani MN,Tilelli CQ,Ribeiro VDS,Goulart LR,Costa-Cruz JM

    更新日期:2020-10-01 00:00:00

  • Capture and penetration processes of the free-living juveniles of Trichostrongylus colubriformis (Nematoda) by the nematophagous fungus, Arthrobotrys oligospora.

    abstract::The nematode-trapping fungus Arthrobotrys oligospora traps and invades all the free-living juvenile stages of the trichostrongyle nematode Trichostrongylus colubriformis. The processes of capture and invasion of the 1st-stage juveniles are described using differential interference contrast optics and 3-D reconstructio...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000079798

    authors: Murray DS,Wharton DA

    更新日期:1990-08-01 00:00:00

  • Physiological cost induced by the maternally-transmitted endosymbiont Wolbachia in the Drosophila parasitoid Leptopilina heterotoma.

    abstract::Endosymbiotic bacteria of the genus Wolbachia infect a number of invertebrate species in which they induce various alterations in host reproduction, mainly cytoplasmic incompatibility (CI). In contrast to most other maternally transmitted parasites, manipulation of host reproduction makes the spread of Wolbachia possi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099006599

    authors: Fleury F,Vavre F,Ris N,Fouillet P,Boulétreau M

    更新日期:2000-11-01 00:00:00

  • Comparative efficacy of immunization with inactivated whole tachyzoites versus a tachyzoite-bradyzoite mixture against neosporosis in mice.

    abstract::The worldwide economic impact of Neospora caninum infection has caused the development of effective vaccines to become one of the main goals in the field of neosporosis research. In this study, the protection conferred by antigens from inactivated whole tachyzoites (TZ) and a tachyzoite-bradyzoite mixture (TZ-BZ) of N...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182011001156

    authors: Rojo-Montejo S,Collantes-Fernández E,Rodríguez-Marcos S,Pérez-Zaballos F,López-Pérez I,Prenafeta A,Ortega-Mora LM

    更新日期:2011-09-01 00:00:00

  • Similarity and structure of the ectoparasite communities of rockfish species from the southern Chilean coast in a temporal scale.

    abstract::The understanding of the patterns and processes underlying the structure of parasite communities has progressed significantly in the last years; however, much of the evidence available to date on parasite community comes from unconfirmed studies. In this study the ectoparasite communities of 2 related rockfishes, Seba...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006000370

    authors: González MT,Oliva ME

    更新日期:2006-09-01 00:00:00

  • A panel of antigens of muscle larvae of Trichinella spiralis and T. pseudospiralis as revealed by two-dimensional western blot and immunoelectron microscopy.

    abstract::This study characterized antigens of Trichinella spiralis and T. pseudospiralis muscle larvae recognized by mice infected with the worms. Two-dimensional (2-D) Western blot analysis revealed some profile of antigenic peptides including: (1) molecular weight (MW); (2) isoelectric points (pI), (3) reactivity to well-def...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099004187

    authors: Wu Z,Nagano I,Takahashi Y

    更新日期:1999-06-01 00:00:00

  • A novel duplex ddPCR assay for the diagnosis of schistosomiasis japonica: proof of concept in an experimental mouse model.

    abstract::The current World Health Organization strategic plan targets the elimination of schistosomiasis as a public health problem by 2025 and accurate diagnostics will play a pivotal role in achieving this goal. DNA-based detection methods provide a viable alternative to some of the commonly used tests, notably microscopy an...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201700021X

    authors: Weerakoon KG,Gordon CA,Cai P,Gobert GN,Duke M,Williams GM,McManus DP

    更新日期:2017-07-01 00:00:00

  • gamma delta T cells do not play a major role in controlling infection in experimental cysticercosis.

    abstract::Protective immunity against larval Taenia crassiceps has been shown to rely on T cells; however, the roles of the specific subsets of T cells during infection are not known. To investigate a possible role for gamma delta T cells, this study investigated larval infection in delta-chain knock-out C57BL/6 (deltaKO) and w...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099004771

    authors: Toenjes SA,Spolski RJ,Mooney KA,Kuhn RE

    更新日期:1999-10-01 00:00:00

  • Label-free enrichment of avian Leucocytozoon using flow cytometric sorting.

    abstract::The group of haemosporidian parasites is of general interest to basic and applied science, since several species infect mammals, leading to malaria and associated disease symptoms. Although the great majority of haemosporidian parasites appear in bird hosts, as in the case of Leucocytozoon buteonis, there is little ge...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182012000972

    authors: Chakarov N,Greiner JF,Hauser S,Schuetzmann D,Krüger O,Boerner M,Wıdera D

    更新日期:2012-10-01 00:00:00

  • Experimental mixed infection of Leishmania (Leishmania) amazonensis and Leishmania (L.) infantum in hamsters (Mesocricetus auratus).

    abstract::In South America, visceral leishmaniasis is frequently caused by Leishmania infantum and, at an unknown frequency, by Leishmania amazonensis. Therefore, mixed infections with these organisms are possible. Mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the d...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017000464

    authors: DE Lima Celeste JL,Venuto Moura AP,França-Silva JC,Matos DE Sousa G,Oliveira Silva S,Norma Melo M,Luiz Tafuri W,Carvalho Souza C,Monteiro DE Andrade H

    更新日期:2017-08-01 00:00:00

  • The virus infecting the parasitoid Leptopilina boulardi exerts a specific action on superparasitism behaviour.

    abstract::Parasites often induce behavioural changes in their host. However, it is not necessarily easy to determine whether these changes are representative of an adaptation of the parasite (parasite manipulation), an adaptive response of the host or a side-effect of infection. In a solitary parasitoid of Drosophila larvae (Le...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006009930

    authors: Varaldi J,Petit S,Boulétreau M,Fleury F

    更新日期:2006-06-01 00:00:00

  • Trypanosoma congolense: appearance and distribution of variable antigen types during metacyclic differentiation in vitro.

    abstract::Differentiation of epimastigotes and production of infective metacyclic forms of Trypanosoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:

    authors: Prain CJ,Ross CA

    更新日期:1990-02-01 00:00:00

  • The formation of the first-stage cuticle within the egg of Trichostrongylus colubriformis.

    abstract::The ultrastructure of the formation of the first-stage cuticle within the egg of Trichostrongylus colubriformis is described. The first indication of cuticle formation was the formation of the epicuticle external to the epidermal plasma membrane. This becomes thickened to form the epicuticle of the fully formed cuticl...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000058868

    authors: Wharton DA,Bone LW

    更新日期:1988-12-01 00:00:00

  • Molecular cloning and characterization of Echinostoma caproni heat shock protein-70 and differential expression in the parasite derived from low- and high-compatible hosts.

    abstract::We cloned and expressed Echinostoma caproni HSP70 in Escherichia coli. This molecule presents an open reading frame (ORF) of 655 amino acids, and a theoretical molecular weight of 71 kDa. E. caproni HSP70 protein showed a high homology to other helminth molecules, major differences being located in the C-terminal regi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008004927

    authors: Higón M,Monteagudo C,Fried B,Esteban JG,Toledo R,Marcilla A

    更新日期:2008-10-01 00:00:00