Abstract:
:Heparin-binding proteins (HBPs) play a key role in Trypanosoma cruzi-host cell interactions. HBPs recognize heparan sulfate (HS) at the host cell surface and are able to induce the cytoadherence and invasion of this parasite. Herein, we analysed the biochemical properties of the HBPs and also evaluated the expression and subcellular localization of HBPs in T. cruzi trypomastigotes. A flow cytometry analysis revealed that HBPs are highly expressed at the surface of trypomastigotes, and their peculiar localization mainly at the flagellar membrane, which is known as an important signalling domain, may enhance their binding to HS and elicit the parasite invasion. The plasmon surface resonance results demonstrated the stability of HBPs and their affinity to HS and heparin. Additionally, gelatinolytic activities of 70 kDa, 65·8 kDa and 59 kDa HBPs over a broad pH range (5·5-8·0) were revealed using a zymography assay. These proteolytic activities were sensitive to serine proteinase inhibitors, such as aprotinin and phenylmethylsulfonyl fluoride, suggesting that HBPs have the properties of trypsin-like proteinases.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Oliveira FO Jr,Alves CR,Silva FS,Côrtes LM,Toma L,Bouças RI,Aguilar T,Nader HB,Pereira MCdoi
10.1017/S0031182012001448subject
Has Abstractpub_date
2013-02-01 00:00:00pages
171-80issue
2eissn
0031-1820issn
1469-8161pii
S0031182012001448journal_volume
140pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported a...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000055256
更新日期:1982-10-01 00:00:00
abstract::Interactions between infections of Trichinella spiralis and Nippostrongylus brasiliensis were studied in the NIH strain of mouse which is known to react strongly to T. spiralis. The course of N. brasiliensis infection in this strain of mouse is described and expulsion is shown to be accelerated in immunized mice and i...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000000512
更新日期:1980-02-01 00:00:00
abstract::Strongyloidiasis is frequently asymptomatic and diagnosis of latent infection is difficult due to limitations of current parasitological and serological methods. This study aimed to verify the use of conventional polymerase chain reaction (PCR) assay for molecular diagnosis of Strongyloides stercoralis infection. Fres...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013002035
更新日期:2014-04-01 00:00:00
abstract::Mice fed 1.5 mg ochratoxin A (OTA) per kg body weight and infected with Trypanosoma brucei rhodesiense were compared with trypanosome-infected placebo-fed and uninfected OTA-fed controls. Uninfected OTA-fed mice showed fever, lethargy, facial and eyelid oedemas, mild hepatitis and nephritis, and high survival. Infecte...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008005386
更新日期:2009-03-01 00:00:00
abstract::The complete nucleotide sequence of the mitochondrial genome was determined for the fish tapeworm Diphyllobothrium latum. This genome is 13,608 bp in length and encodes 12 protein-coding genes (but lacks the atp8), 22 transfer RNA (tRNA) and 2 ribosomal RNA (rRNA) genes, corresponding to the gene complement found thus...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118200600206X
更新日期:2007-05-01 00:00:00
abstract::Receptors interacting with terminal sugars as ligands are involved in the binding of Leishmania donovani promastigotes to the macrophage surface and their subsequent internalization. Mannose and glucose are specifically involved in the binding process. Decreased binding occurs to macrophages already infected with L. d...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076253
更新日期:1994-08-01 00:00:00
abstract::Pinewood nematode, Bursaphelenchus xylophilus, a pine parasitic nematode, poses a serious threat to its host pine forests globally. When dispersal-stage larvae 4 (dauer, DL4) of B. xylophilus enters the new pine, it moults into propagative adult (dauer recovery) and reproduces quickly to kill the host pine. Here, we f...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019001264
更新日期:2020-01-01 00:00:00
abstract::Biodiversity loss may increase the risk of infectious disease in a phenomenon known as the dilution effect. Circumstances that increase the likelihood of disease dilution are: (i) when hosts vary in their competence, and (ii) when communities disassemble predictably, such that the least competent hosts are the most li...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182020000943
更新日期:2020-09-01 00:00:00
abstract::Harnessing the full utility of extensive gene sequences recently available for the economically important sea louse, Lepeophtheirus salmonis, requires the adaptation of modern molecular biology approaches to this non-model organism. Using a putative microsomal prostaglandin E synthase type-2 (PGES2) as a candidate gen...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009990357
更新日期:2009-07-01 00:00:00
abstract::Dr David Livingstone died on May 1st 1873. He was 60 years old and had spent much of the previous 30 years walking across large stretches of Southern Africa, exploring the terrain he hoped could provide new environments in which Europeans and Africans could cohabit on equal terms and bring prosperity to a part of the ...
journal_title:Parasitology
pub_type: 传,历史文章,杂志文章
doi:10.1017/S003118201600202X
更新日期:2017-10-01 00:00:00
abstract::A novel tetrahydroquinolinyl ester, quinfamide, administered orally in multiple doses for 3 days had an ED50 of 0.25 mg/kg/day (total dose 0.75 mg/kg) for eradicating Entamoeba criceti in hamsters in several tests. It was significantly more active by direct comparison than 3 commercially available amoebicides and at l...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000055128
更新日期:1980-08-01 00:00:00
abstract::Humans and other hosts acquire Toxoplasma gondii infection by ingesting tissue cysts in undercooked meat, or by food or drink contaminated with oocysts. Currently, there is no vaccine to prevent clinical disease due this parasite in humans, although, various T. gondii vaccine candidates are being developed. Mice are g...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001673
更新日期:2012-01-01 00:00:00
abstract::Treatment of BALB/c or MF1 mice with cyclosporin A (CsA) around the time of infection with Schistosoma mansoni conferred almost complete protection. The migration kinetics of L-[75Se]selenomethionine-labelled infective cercariae were investigated by compressed tissue autoradiography. Similar levels of skin penetration...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060340
更新日期:1991-02-01 00:00:00
abstract::Transmission experiments were conducted to compare the transmissibility of genetically different Trypanosoma congolense (Savannah subgroup) strains isolated from cattle in a trypanosomiasis endemic area of eastern Zambia. A total of 17 strains were compared. Three strains were extremely virulent with a short pre-paten...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006000369
更新日期:2006-09-01 00:00:00
abstract::Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular laye...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006000400
更新日期:2006-09-01 00:00:00
abstract::Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process of Ascaris suum larvae. This study describes moulting and development-specific protein expression profiles of A. suum lung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepa...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006000576
更新日期:2006-10-01 00:00:00
abstract::Human cysticercosis is a public health problem caused by Taenia solium metacestodes; thus, eradication of T. solium transmission by vaccination is an urgent requirement. The Cc48 mimotope from T. solium cysticerci was tested expressed in phage particles (mCc48) and chemically synthesized (sCc48) as a vaccine candidate...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182020001080
更新日期:2020-10-01 00:00:00
abstract::The nematode-trapping fungus Arthrobotrys oligospora traps and invades all the free-living juvenile stages of the trichostrongyle nematode Trichostrongylus colubriformis. The processes of capture and invasion of the 1st-stage juveniles are described using differential interference contrast optics and 3-D reconstructio...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000079798
更新日期:1990-08-01 00:00:00
abstract::Endosymbiotic bacteria of the genus Wolbachia infect a number of invertebrate species in which they induce various alterations in host reproduction, mainly cytoplasmic incompatibility (CI). In contrast to most other maternally transmitted parasites, manipulation of host reproduction makes the spread of Wolbachia possi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099006599
更新日期:2000-11-01 00:00:00
abstract::The worldwide economic impact of Neospora caninum infection has caused the development of effective vaccines to become one of the main goals in the field of neosporosis research. In this study, the protection conferred by antigens from inactivated whole tachyzoites (TZ) and a tachyzoite-bradyzoite mixture (TZ-BZ) of N...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001156
更新日期:2011-09-01 00:00:00
abstract::The understanding of the patterns and processes underlying the structure of parasite communities has progressed significantly in the last years; however, much of the evidence available to date on parasite community comes from unconfirmed studies. In this study the ectoparasite communities of 2 related rockfishes, Seba...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006000370
更新日期:2006-09-01 00:00:00
abstract::This study characterized antigens of Trichinella spiralis and T. pseudospiralis muscle larvae recognized by mice infected with the worms. Two-dimensional (2-D) Western blot analysis revealed some profile of antigenic peptides including: (1) molecular weight (MW); (2) isoelectric points (pI), (3) reactivity to well-def...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099004187
更新日期:1999-06-01 00:00:00
abstract::The current World Health Organization strategic plan targets the elimination of schistosomiasis as a public health problem by 2025 and accurate diagnostics will play a pivotal role in achieving this goal. DNA-based detection methods provide a viable alternative to some of the commonly used tests, notably microscopy an...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118201700021X
更新日期:2017-07-01 00:00:00
abstract::Protective immunity against larval Taenia crassiceps has been shown to rely on T cells; however, the roles of the specific subsets of T cells during infection are not known. To investigate a possible role for gamma delta T cells, this study investigated larval infection in delta-chain knock-out C57BL/6 (deltaKO) and w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099004771
更新日期:1999-10-01 00:00:00
abstract::The group of haemosporidian parasites is of general interest to basic and applied science, since several species infect mammals, leading to malaria and associated disease symptoms. Although the great majority of haemosporidian parasites appear in bird hosts, as in the case of Leucocytozoon buteonis, there is little ge...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182012000972
更新日期:2012-10-01 00:00:00
abstract::In South America, visceral leishmaniasis is frequently caused by Leishmania infantum and, at an unknown frequency, by Leishmania amazonensis. Therefore, mixed infections with these organisms are possible. Mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the d...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017000464
更新日期:2017-08-01 00:00:00
abstract::Parasites often induce behavioural changes in their host. However, it is not necessarily easy to determine whether these changes are representative of an adaptation of the parasite (parasite manipulation), an adaptive response of the host or a side-effect of infection. In a solitary parasitoid of Drosophila larvae (Le...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006009930
更新日期:2006-06-01 00:00:00
abstract::Differentiation of epimastigotes and production of infective metacyclic forms of Trypanosoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them...
journal_title:Parasitology
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::The ultrastructure of the formation of the first-stage cuticle within the egg of Trichostrongylus colubriformis is described. The first indication of cuticle formation was the formation of the epicuticle external to the epidermal plasma membrane. This becomes thickened to form the epicuticle of the fully formed cuticl...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000058868
更新日期:1988-12-01 00:00:00
abstract::We cloned and expressed Echinostoma caproni HSP70 in Escherichia coli. This molecule presents an open reading frame (ORF) of 655 amino acids, and a theoretical molecular weight of 71 kDa. E. caproni HSP70 protein showed a high homology to other helminth molecules, major differences being located in the C-terminal regi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008004927
更新日期:2008-10-01 00:00:00