Abstract：
:Using rat model of spinal cord contusion injury at TVIII, we compared the effectiveness of immediate single transplantation of human mononuclear umbilical cord blood cells transfected with pBud-VEGF-FGF2 plasmid and immediate direct injection of the same plasmid into the lesion area. The results suggest that the delivery of therapeutic genes vegf and fgf2 in cells is more effective than direct injection of plasmid DNA with the same genes (judging from the number of myelinated fibers). Better tissue preservation and motor function recovery in experiments with direct injection of plasmid pBud-VEGF-FGF2 suggest that direct gene therapy seems to be an effective additional procedure to the method of gene delivery with transfected stem and progenitor cells.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Shaymardanova GF,Mukhamedshina YO,Salafutdinov II,Rizvanov AA,Chelyshev YAdoi
10.1007/s10517-013-1996-5subject
Has Abstractpub_date
2013-02-01 00:00:00pages
544-7issue
4eissn
0007-4888issn
1573-8221journal_volume
154pub_type
杂志文章abstract：:The role of extracellular matrix enzymes in the development of distant metastases of colorectal cancer was studied. The expression of types 2 and 9 matrix metalloproteinases was evaluated in primary colorectal adenocarcinomas (264 patients) and their metastases in the lymph nodes (127 patients) and liver (109 patients...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-009-0507-1
更新日期：2009-03-01 00:00:00
abstract：:Kinetics of DNA synthesis in mitotic cycle of in mouse corneal epithelial cells after γ-irradiation in different S phase points was studied by the method of autoradiography. Normally, S phase of corneal epithelial cells consists of two phases (S1 and S2) separated by an interval without DNA synthesis. Each phase, in t...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2715-6
更新日期：2014-11-01 00:00:00
abstract：:Hemopoietic islets, associations of stromal (macrophages, fibroblasts) and blood (including stem) cells, are structural and functional units of the bone marrow. We studied cellular and molecular processes developing following short-term (1 h) contact of hemopoietic islets with ferromagnetic nanoparticles in a multicel...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-011-1360-6
更新日期：2011-08-01 00:00:00
abstract：:Transplantation of erythroid and bone marrow cells to irradiated mice stimulated exogenous colony formation. Pretreatment of erythroid cells with specific rabbit antiserum to erythroblasts abolished this effect. The reverse transcriptase polymerase chain reaction revealed the presence of mRNA for interleukin-1alpha, i...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:One-month therapy with trimetazidine sharply decreased the content of free radical oxidation products, lipid peroxides and malonic dialdehyde, in atherogenic low-density lipoproteins in patients with coronary heart disease. Activity of glutathione peroxidase utilizing lipid peroxides in the plasma markedly increased d...
journal_title：Bulletin of experimental biology and medicine
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Experiments on stationary culture of posterior eye and roll-bottle culture of the whole eye from adult water lizards Pleurodeles waltl showed that sclera bioregulator produces a stabilizing effects on adhesion interactions between the sclera, choroid, and pigment epithelium and on the maintenance of viability of scler...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-010-0957-5
更新日期：2010-10-01 00:00:00
abstract：:Age-associated changes in the concentrations of glucose, insulin, and DHEAS in peripheral blood plasma of female rhesus macaques were studied in intact animals and in response to a standard dose of glucose and insulin. Basal levels of insulin and glucose increased, insulin sensitivity and glucose tolerance decreased, ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/b:bebm.0000031570.81043.f9
更新日期：2004-03-01 00:00:00
abstract：:Castration had no effect on baseline BP and vascular sensitivity to acetylcholine and deficiency of nitric oxide and prostacyclin in normotensive specimens. Castration of hypertensive specimens decreased BP and potentiated the hypotensive effects of acetylcholine, but did not modulate vascular sensitivity to the block...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-012-1673-0
更新日期：2012-06-01 00:00:00
abstract：:We studied the effect of electroconvulsive therapy on cognitive functions in rats with depression-like disorder caused by exposure to ultrasound of varying frequency (20-45 kHz). Object recognition and Morris water-maze tests revealed no negative effects of the therapy on memory. Moreover, positive effect of therapy w...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-017-3857-0
更新日期：2017-09-01 00:00:00
abstract：:Treadmill training of spinalized rats creates conditions for early appearance of rhythmic locomotor movements of the hind limbs. Recovery of the movements was paralleled by an appropriate structural organization of neurons in the anterior horns of the distal compartment of the spinal cord. ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/b:bebm.0000048388.76324.9c
更新日期：2004-08-01 00:00:00
abstract：:Antisense oligonucleotide to alpha2A-adrenoceptors increased the levels of mRNA (reverse polymerase chain reaction) and protein for a key executioner protease of apoptosis caspase-3 (immunoblotting assay) in the cerebral cortex of newborn rats. The relationship between the observed effect and low expression of alpha2-...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-007-0088-9
更新日期：2007-03-01 00:00:00
abstract：:We proposed new method for measuring dynamic changes in hunger motivation in rabbits in the course of satisfaction of nutritional need by weight, time, and rate parameters of effective food-procuring behavior. Transformation of the amount of food eaten into the electric signals was performed using electronic weighing ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-012-1629-4
更新日期：2012-05-01 00:00:00
abstract：:Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-017-3785-z
更新日期：2017-06-01 00:00:00
abstract：:We studied the relationship between the length and tension in isolated strips of the capsules of bovine mesenteric lymph nodes. The deformation-strain and radius-pressure relationships were established in the lymph node. The capsule possesses high distensibility, modulus of elasticity at optimal tension was 0.09×10(5)...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-011-1246-7
更新日期：2011-05-01 00:00:00
abstract：:Apoptosis of blood neutrophils from healthy donors was studied under conditions of cell culturing with different concentrations of H(2)O(2), selective NO synthase inhibitor, and inductor of NO synthesis (L-arginine). In vitro incubation of neutrophilic leukocytes with 5 mM H(2)O(2) led to activation of the apoptotic p...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-009-0386-5
更新日期：2008-12-01 00:00:00
abstract：:We studied the effect of four conjugated synthetic derivatives of estrone and ethynylestradiol and bis-beta-chloroethylamine-containing substance on activity of plasma membrane enzymes 5'-nucleotidase and N+-K+-ATPase. As differentiated from precursors, estrogen cytostatics decreased activity of plasma membrane enzyme...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/b:bebm.0000020202.89379.f3
更新日期：2003-12-01 00:00:00
abstract：:Dysregulation of neuroimmune connections is a primary or secondary pathogenic factor of some CNS diseases. Autoimmune aggression is typical of multiple sclerosis, Alzheimer's disease, and epilepsy, while dysregulation characterized by enhanced production of autoantibodies to neurotransmitters and activation of cell fa...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章,评审
doi：10.1023/a:1017964229295
更新日期：2001-04-01 00:00:00
abstract：:Newborn rats received intraperitoneal injections of endothelin-1 in a daily dose of 5 x 10(-8)mol/kg 30 min after administration of NG-nitro-L-arginine methyl ester (9.3 x 10(-5)mol/kg intraperitoneally) from the 2nd to 6th day of life. NADPH diaphorase activity in epithelial and smooth muscle cells of the tracheobron...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1015826415361
更新日期：2002-03-01 00:00:00
abstract：:Plasma concentration of immune complexes and antibodies to serotonin and dopamine were higher in patients with Alzheimer's disease compared to mentally healthy volunteers of the same age. Plasma concentrations of antibodies to serotonin and dopamine decreased with the increase in the duration of this disease. ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1020692218416
更新日期：2002-07-01 00:00:00
abstract：:Carnosine significantly increased the number of spermatogonia and Sertoli cells in mice prone (SAMP1) and resistant (SAMR1) to accelerated aging and appreciably reduced cell yield in meiosis and spermiogenesis in SAMP1 mice. In experimental SAMP1 mice catastrophic changes in the number of gametes were paralleled by in...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-005-0454-4
更新日期：2005-08-01 00:00:00
abstract：:Loading of neutrophils with immune complexes changed their functional activity and intracellular pH. These changes depended on the size of immune complexes. UV irradiation of neutrophils loaded with immune complexes led to a molecular weight-dependent decrease in intracellular pH. After irradiation the chemiluminescen...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1013174802845
更新日期：2001-09-01 00:00:00
abstract：:We studied some structural and functional parameters of erythrocyte membranes in mice at the late presymptomatic and early symptomatic stages of experimental Parkinson's disease induced by administration of MPTP (hemolysis, microviscosity of different regions of the lipid bilayer, LPO intensity, activity of antioxidan...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-017-3666-5
更新日期：2017-03-01 00:00:00
abstract：:Immunocytochemical study has shown that Ki-67 antigen is detected in adenomyosis in both endometrial and myometrial cell populations (in the eutopic and ectopic endometrial glandular epithelium, stromal cells, smooth muscle cells, and vascular endotheliocytes of the endometrium and myometrium), the label index differi...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-2190-5
更新日期：2013-08-01 00:00:00
abstract：:Nadcin in a dose of 90 mg/kg administered to dogs with subacute stage of ischemia-reperfusion myocardial injury immediately after blood flow resumption normalized redox potential of cardiomyocytes and mitochondria and restored the total content of adenyl and pyridine nucleotides. The decrease in the synthesis of ATP a...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-008-0268-2
更新日期：2008-09-01 00:00:00
abstract：:The rats with neurodegenerative brain disorder induced by administration of a toxic fragment of beta-amyloid demonstrate weakened endothelium-dependent dilation of cerebral vessels, which attested to impaired production of endothelial NO. At the same time, toxic beta-amyloid fragment induced the formation of NO depots...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-006-0318-6
更新日期：2006-08-01 00:00:00
abstract：:We studied a biomaterial for a new domestic product, a biological envelope for implantation of cardiac electronic devices. The product is designed to prevent complications after pacemaker implantation and to facilitate the reimplantation procedure. By chemical and biological processing of raw materials (submucosa of p...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-020-04712-0
更新日期：2020-01-01 00:00:00
abstract：:Human umbilical cord represents a source of multipotent stromal cells of a supreme therapeutic potential. The cells can be isolated from either fresh or cryopreserved umbilical cord tissues. DMSO is a cryoprotectant most commonly used for preservation of umbilical cord tissues; however, cyto- and genotoxicity of this ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-018-4305-5
更新日期：2018-11-01 00:00:00
abstract：:The area, thickness, and volume of erythrocytes of different types (discocytes, stomatocytes, and echinocytes) from normal subjects and coronary patients were studied by laser interference microscopy. Increase of pH value leading to the stomatocyte-discocyte-echinocyte transformations resulted in a slight decrease of ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-008-0097-3
更新日期：2008-03-01 00:00:00
abstract：:We studied the effect of melatonin on the relationship between LPO intensity and proteolytic activity in basal nuclei (caudate nucleus, globus pallidus, amygdaloid complex, and nucleus accumbens septi) of rat brain during acute hypobaric hypoxia. Acute hypoxia was accompanied by LPO activation and increase in proteoly...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-006-0298-6
更新日期：2006-07-01 00:00:00
abstract：:Signal pathways of realization of growth potential of mesenchymal progenitor cells related to transcription factor NF-κB were studied in vitro. NF-κB was found to participate in the proliferation and differentiation of progenitor elements that can be blocked by its specific inhibitor oridonin. NF-κB inhibitor aurothio...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-2236-8
更新日期：2013-10-01 00:00:00
