Usage of plasmid vector carrying vegf and fgf2 genes after spinal cord injury in rats.

Abstract:

:Using rat model of spinal cord contusion injury at TVIII, we compared the effectiveness of immediate single transplantation of human mononuclear umbilical cord blood cells transfected with pBud-VEGF-FGF2 plasmid and immediate direct injection of the same plasmid into the lesion area. The results suggest that the delivery of therapeutic genes vegf and fgf2 in cells is more effective than direct injection of plasmid DNA with the same genes (judging from the number of myelinated fibers). Better tissue preservation and motor function recovery in experiments with direct injection of plasmid pBud-VEGF-FGF2 suggest that direct gene therapy seems to be an effective additional procedure to the method of gene delivery with transfected stem and progenitor cells.

journal_name

Bull Exp Biol Med

authors

Shaymardanova GF,Mukhamedshina YO,Salafutdinov II,Rizvanov AA,Chelyshev YA

doi

10.1007/s10517-013-1996-5

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

544-7

issue

4

eissn

0007-4888

issn

1573-8221

journal_volume

154

pub_type

杂志文章
  • Tartrate-resistant acid phosphatase as a marker of bone metastases in patients with breast cancer and prostate cancer.

    abstract::Serum activity of tartrate-resistant acid phosphatase 5b (TRAP 5b) in patients with breast cancer and prostate cancer having bone metastases was much higher than in healthy donors and patients without skeletal injuries. TRAP 5b activity in patients with breast cancer and multiple bone metastases surpassed that in pati...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1023/b:bebm.0000046945.95479.d6

    authors: Lyubimova NV,Pashkov MV,Tyulyandin SA,Gol'dberg VE,Kushlinskii NE

    更新日期:2004-07-01 00:00:00

  • Lipid spectrum in fractions of liver cell chromatin from mice after partial hepatectomy.

    abstract::The qualitative and quantitative differences in lipids composition were found in chromatin fractions differing by attachment to the nuclear matrix and transcriptional activity. During liver regeneration after partial hepatectomy Changes in the lipid spectrum of chromatin fractions in G1- and S-phases are associated wi...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-006-0098-z

    authors: Trofimov VA,Dudko AA

    更新日期:2006-01-01 00:00:00

  • Spontaneous aneuploidy and clone formation in adipose tissue stem cells during different periods of culturing.

    abstract::Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fluorescent in situ hybridization. During the early passages, monosomal cells were ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-009-0647-3

    authors: Buyanovskaya OA,Kuleshov NP,Nikitina VA,Voronina ES,Katosova LD,Bochkov NP

    更新日期:2009-07-01 00:00:00

  • Key enzymes of the extracellular matrix in colorectal cancer.

    abstract::The role of extracellular matrix enzymes in the development of distant metastases of colorectal cancer was studied. The expression of types 2 and 9 matrix metalloproteinases was evaluated in primary colorectal adenocarcinomas (264 patients) and their metastases in the lymph nodes (127 patients) and liver (109 patients...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-009-0507-1

    authors: Golovkov DA

    更新日期:2009-03-01 00:00:00

  • Immunohistochemical Phenotypes of Stable and Unstable Occlusive Atherosclerotic Plaques in Coronary Arteries.

    abstract::We performed a complex morphological analysis of atherosclerotic plaques obtained from 68 men with coronary atherosclerosis during coronary bypass surgery with endarterectomy. The expression of MMP-2 and MMP-9, collagen IV, CD31, CD34, factor VIII, and of smooth muscle cell actin was measured in the samples by morphom...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-018-4268-6

    authors: Murashov IS,Volkov AM,Kazanskaya GM,Kliver EE,Savchenko SV,Klochkova SV,Lushnikova EL

    更新日期:2018-10-01 00:00:00

  • Dominant and Non-Dominant Structure of Ventricular Fibrillation in Canine Heart.

    abstract::Organized activity of the myocardium manifested in dominant frequency structure of EСG was typical of the first 10 min of ventricular fibrillation in canine heart. The first minute of fibrillation was characterized by the most pronounced changes in the structure of oscillation frequency and transition from domination ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-016-3153-4

    authors: Guryanov MI

    更新日期:2016-01-01 00:00:00

  • Antimetastatic effect of thymus-dependent antigen (sheep erythrocytes) in C57BL/6 mice with Lewis carcinoma.

    abstract::We revealed an antimetastatic effect of thymus-dependent corpuscular antigen (sheep erythrocytes) injected intravenously or intraperitoneally in sensitizing or high doses alone or in a complex with a course dose of cyclophosphamide to C57Bl/6 mice with Lewis carcinoma. Injection of the antigen appreciably reduced the ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1023/b:bebm.0000042717.00428.e9

    authors: Mosienko MD,Mosienko VS,Solyanik GI,Kovalenko NG

    更新日期:2004-06-01 00:00:00

  • Experimental model of syringomyelia in rabbits.

    abstract::We studied the possibility of reproducing syringomyelia in rabbits by injection of serum from patients with syringomyelia. Clinical signs of syringomyelia and morphological changes in the central nervous system (cavities, dilatation of the cerebrospinal channel, neurodegeneration, gliosis) developed in laboratory anim...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-007-0166-z

    authors: Mirsayev TR,Borisova NA

    更新日期:2007-04-01 00:00:00

  • Evaluation of genotoxicity and reproductive toxicity of silicon nanocrystals.

    abstract::Silicon crystal 2-5 nm nanoparticles in the form of 1-5-μ granules in water suspension were injected intraperitoneally in a single dose to male F(1)(CBA×C57Bl/6) mice or to outbred albino rats on days 1, 7, and 14 of gestation. Silicon crystal nanoparticles in doses of 5, 25, and 50 mg/kg exhibited no cytogenetic acti...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-010-0967-3

    authors: Durnev AD,Solomina AS,Daugel-Dauge NO,Zhanataev AK,Shreder ED,Nemova EP,Shreder OV,Veligura VA,Osminkina LA,Timoshenko VY,Seredenin SB

    更新日期:2010-10-01 00:00:00

  • Effect of interleukin-1beta on the behavior of rats during mild stress in the open-field test.

    abstract::We studied the effect of interleukin-1beta on the behavior of rats with different individual typological characteristics during mild stress in the open-field test. Intraperitoneal injection of interleukin-1beta (5 microg/kg, 108 U/mg) was followed by a decrease in orientation and exploratory activity of passive and, p...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-010-0804-8

    authors: Pertsov SS,Koplik EV,Simbirtsev AS,Kalinichenko LS

    更新日期:2009-11-01 00:00:00

  • Antibacterial Activity of L-Lysine-α-Oxidase from the Trichoderma.

    abstract::We studied the effects of a concentrate of metabolites of Trichoderma harzianum Rifai F-180, an active producer of L-lysine-α-oxidase, and homogenous enzyme on a highly virulent bacteria Erwinia amylovora. The producer of antitumor and antiviral Trichoderma enzyme L-lysine-α-oxidase was cultured on a processing system...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-017-3901-0

    authors: Smirnova IP,Karimova EV,Shneider YA

    更新日期:2017-10-01 00:00:00

  • The Effect of Azithromycin on Biofilms Formation by Pathogens of Implant-Associated Infection in Large Joints.

    abstract::We studied the effect of subbacteriostatic azithromycin concentrations on the formation of microbial biofilms by Pseudomonas aeruginosa strains that caused implant-associated infection of large joints. Azithromycin in subinhibitory for planktonic cells concentrations 0.01-0.02 μg/ml stimulated biofilm formation by bot...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-020-04982-8

    authors: Babushkina IV,Ulyanov VY,Mamonova IA,Shpinyak SP

    更新日期:2020-10-01 00:00:00

  • Bioactive 5-reduced 16 alpha,17 alpha-cyclohexanoprogesterone derivatives weakly interact with proteins of the soluble uterine fraction.

    abstract::We studied competitive activities of 16 alpha,17 alpha-cyclohexano-5 alpha- and 5 beta-dihydroprogesterone in replacing(3)H-progesterone and(3)H-16 alpha,17 alpha-cyclohexano-6 alpha-methylprogesterone from protein complexes. Direct binding of(3)H-5-reduced derivatives with proteins of soluble fractions from rat and r...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1023/a:1017647331285

    authors: Smirnov AN,Pokrovskaya EV,Levina IS,Kulikova LE,Kamernitskii AV,Shevchenko VP

    更新日期:2001-03-01 00:00:00

  • Lymphoprotective effect of perftoran.

    abstract::Experiments on rats showed that the lymphoprotective effect of Perftoran manifests in stimulation of contractile activity of myocytes in the wall and valve cusps of lymphatic microvessels, which in turn activates the pump and capacitive functions of the lymphatic system and improves central lymph outflow. These change...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-010-1036-7

    authors: Mukhutdinova FI,Khafiz'janova RKh,Triandafilov KA,Mukhutdinov DA

    更新日期:2010-11-01 00:00:00

  • Poly(ADP-ribose)-polymerase-1 and aging: experimental study of possible relationship on stationary cell cultures.

    abstract::Experiments on "stationary aging" cultures of B11dii-FAF28 Chinese hamster cells showed that contact inhibition of cell growth and further culturing of cells in the stationary phase led to continuous inhibition of enzymes realizing poly-ADP-ribosylation of chromatin proteins. Cell density in the monolayer and percenta...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-006-0238-5

    authors: Shram SI,Shilovskii GA,Khokhlov AN

    更新日期:2006-05-01 00:00:00

  • Formation of morphine tolerance in offspring of morphine-tolerant animals: neurochemical and neuroimmune correlates.

    abstract::We carried out a complex physiological, neurochemical, and neuroimmunologic study of the formation of tolerance to analgetic effect of morphine and analyzed enkephalinase A activity in different brain structures and serotonin antibodies in the serum. More early development of morphine tolerance and a sharp increase in...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1023/a:1017578528866

    authors: Litvinova SV,Aristova VV,Shul'govskii VV,Basharova LA,Terebilina NN,Panchenko LF

    更新日期:2001-01-01 00:00:00

  • Evaluation of serum antioxidant activity in patients with liver pathology by the chemiluminescent method.

    abstract::Total antioxidant activity of the serum in patients with liver pathology was assessed by two chemiluminescent methods based on different models of free radical oxidation: Hb-H2O2-luminol and ABAP-luminol. Comparative analysis showed a significant, but not high correlation of the results (r=0.798), which can be explain...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-014-2742-3

    authors: Titov VN,Sazhina NN

    更新日期:2014-12-01 00:00:00

  • Effect of benz(a)pyrene and constant light exposure on rat liver lysosomes and biliary excretion of lysosomal enzymes.

    abstract::Threefold administration of benz(a)pyrene in a dose of 20 mg/kg markedly stimulated biliary excretion of lysosomal enzymes from rat liver without signs of lysosomal damage. Constant light exposure induced changes attesting to functional activation of the lysosomal apparatus in liver cells and inhibited constitutive bi...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-005-0205-6

    authors: Pupyshev AB,Gutina EM,Fedina RG,Michurina SV,Shurlygina AV,Verbitskaya LV

    更新日期:2005-01-01 00:00:00

  • Psychoimmune interactions in women of reproductive age with endometriosis.

    abstract::Psychoimmune interactions were studied in women of reproductive age with endometriosis. Pronounced immunological shifts manifested in a shift of the T-cellular immunity, resulting in imbalanced production of pro- (IL-1β, IL-2, IFN-γ) and anti-inflammatory (IL-4) cytokines. Significant correlations between the severity...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-011-1463-0

    authors: Nasyrova RF,Sotnikova LS,Baystrukova NV,Krivoschchekova GV,Novitsky VV,Kupriyanova IE,Semke VY,Naslednikova IO,Baykov AN

    更新日期:2011-11-01 00:00:00

  • Analysis of the Expression of Regulator Genes in Kupffer Cells and Monocytes.

    abstract::Differences in the gene expression profiles in resident macrophages (in particular, Kupffer cells) and monocytes were revealed. However, these differences in gene expression profiles do not allow considering resident liver macrophages as purely M2 macrophages and monocytes as purely M1 macrophages. At the same time, a...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-020-04752-6

    authors: Nikitina MP,Elchaninov AV,Lokhonina AV,Makarov AV,Tagirova MK,Grinberg MV,Bolshakova GB,Glinkina VV,Goldshtein DV,Fatkhudinov TK

    更新日期:2020-02-01 00:00:00

  • Relationship between oxidized fibrinogen and hemostasis disturbances and endothelial dysfunction in myocardial infarction.

    abstract::Oxidative modification of fibrinogen in acute myocardial infarction increased 1.3-fold compared to that in CHD and 1.5-fold surpassed that in the control without CHD. Elevated content of oxidized fibrinogen correlated with increased levels of LPO products, von Willebrand factor, and fibrin degradation products, with a...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-008-0104-8

    authors: Ragino YI,Baum VA,Polonskaya YV,Sadovski EV,Baum SR,Nikitin YP

    更新日期:2008-04-01 00:00:00

  • Effects of Thermoextracts of Brucella S and L Forms on Lipid Peroxidation and Antioxidant Defense in Organs of Laboratory Animals.

    abstract::The dynamics of LPO marker malondialdehyde formation and peroxidase-destroying activity was studied in homogenized organs of guinea pigs, immunized with thermoextracts from S and L forms Brucella abortus I-206. The L form brucella thermoextract exhibited a lower reactogenicity and adequately activated the antioxidant ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-018-4138-2

    authors: Dubrovina VI,Balakhonov SV,Yurieva OV,Starovoitova TP,Barannikova NL,Tokareva LE,Pyatidesyatnikova AB,Yastremskaya KY,Shkaruba TT

    更新日期:2018-06-01 00:00:00

  • Role of cyclic nucleotides and NO synthase in mechanisms of cardioprotective effects of cannabinoid HU-210.

    abstract::Isolated perfused rat heart was subjected to global ischemia (45 min) followed by reperfusion (30 min). Under these conditions, the level of creatine phosphokinase in the perfusate increased by 4.5 times. Perfusion (10 min) of the isolated heart with a solution containing cannabinoid HU-210 (0.1 or 1.0 μmol/liter) was...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-014-2622-x

    authors: Maslov LN,Krylatov AV,Lishmanov YB

    更新日期:2014-09-01 00:00:00

  • Effect of iron ions on functional activity of thrombin.

    abstract::The kinetics of thrombin inhibition by irons ions was studied in the thrombin time test with normal plasma. The kinetic and concentration characteristics for recovery of thrombin activity by desferal were evaluated at various periods of thrombin incubation with iron ions. The thrombin time test showed that incubation ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-010-0814-6

    authors: Azizova OA,Shvachko AG,Aseichev AV

    更新日期:2009-11-01 00:00:00

  • Activation of Microglyocytes in the Anterior Horns of Rat Spinal Cord after Administration of Bacterial Lipopolysaccharide.

    abstract::We studied the reaction of the microglia of the anterior horns of the rat spinal cord to intraperitoneal administration of bacterial LPS. Immunohistochemical analysis showed that acute systemic inflammation leads to activation of more than half of microglial cells as soon as in 24 h after LPS injection, while the tota...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-017-3841-8

    authors: Kolos EA,Korzhevskii DE

    更新日期:2017-08-01 00:00:00

  • Differentiation of dissociated rat embryonic brain after allotransplantation into damaged nerve.

    abstract::Fragments of the dorsolateral wall of the anterior brain vesicle from rat embryos on embryonic day 15 were dissociated and the resultant suspension containing single cells and cell aggregates was injected into the proximal segment of crushed sciatic nerve of adult animals for evaluation of their engrafting and differe...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-013-2296-9

    authors: Petrova ES,Isaeva EN,Korzhevskii DE

    更新日期:2013-11-01 00:00:00

  • Effect of physical training on blood level of endogenous modulators of beta-adreno- and m-cholinoreactivity in patients with a history of myocardial infarction.

    abstract::The relative content of myocyte-active factors and endogenous muscarinic receptor blocker in the blood increased, while the concentration of endogenous beta-adrenoceptor sensitizer decreased in coronary patients with a history of acute myocardial infarction. Physical training produced a therapeutic effect, normalized ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1023/a:1026068208764

    authors: Tsirkin VI,Nozdrachev AD,Sizova EN,Mal'chikova SV,Gulyaeva SF

    更新日期:2003-07-01 00:00:00

  • Immunostimulatory effect of gold nanoparticles conjugated with transmissible gastroenteritis virus.

    abstract::Animals were immunized with transmissible gastroenteritis virus conjugated with gold nanoparticles. The resultant antibodies had a higher titer than antibodies produced in response to native virus. Immunization with the antigen-colloidal gold complex led to a significant increase of the peritoneal macrophages respirat...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-011-1350-8

    authors: Staroverov SA,Vidyasheva IV,Gabalov KP,Vasilenko OA,Laskavyi VN,Dykman LA

    更新日期:2011-08-01 00:00:00

  • Cell Composition of Central and Peripheral Lymphoid Organs of Wistar Rats Treated with a Biomedical Cell Product.

    abstract::We studied the effect of a biomedical cell product (bone marrow multipotent mesenchymal stromal cells and products secreted by these cells in conditioned medium) on subpopulation composition of lymphoid cells of central and peripheral lymphoid organs of Wistar rats under normal conditions. Changes in the subpopulation...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-016-3366-6

    authors: Konenknov VI,Borodin YI,Dergacheva TI,Shurlygina AV,Mel'nikova EV,Tenditnik MV,Starkova EV,Lykov AP,Poveshchenko OV,Bondarenko NA,Kim II

    更新日期:2016-05-01 00:00:00

  • Heterogeneity of mitochondrial potential as a marker for isolation of pure cardiomyoblast population.

    abstract::Typical signs of cardiomyoblasts were determined: high mitochondrial membrane potential and high number of mitochondria in these cells compared to fibroblasts. These signs can be used for identification of these cells. Energy-dependent accumulation of highly specific mitochondrial fluorescent probes applied for visual...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-009-0327-3

    authors: Khryapenkova TG,Plotnikov EY,Korotetskaya MV,Sukhikh GT,Zorov DB

    更新日期:2008-10-01 00:00:00