Abstract:
:Using rat model of spinal cord contusion injury at TVIII, we compared the effectiveness of immediate single transplantation of human mononuclear umbilical cord blood cells transfected with pBud-VEGF-FGF2 plasmid and immediate direct injection of the same plasmid into the lesion area. The results suggest that the delivery of therapeutic genes vegf and fgf2 in cells is more effective than direct injection of plasmid DNA with the same genes (judging from the number of myelinated fibers). Better tissue preservation and motor function recovery in experiments with direct injection of plasmid pBud-VEGF-FGF2 suggest that direct gene therapy seems to be an effective additional procedure to the method of gene delivery with transfected stem and progenitor cells.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Shaymardanova GF,Mukhamedshina YO,Salafutdinov II,Rizvanov AA,Chelyshev YAdoi
10.1007/s10517-013-1996-5subject
Has Abstractpub_date
2013-02-01 00:00:00pages
544-7issue
4eissn
0007-4888issn
1573-8221journal_volume
154pub_type
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