Abstract:
:A novel conotoxin, pc16a, was isolated from the venom of Conus pictus. This is the first peptide characterized from this South-African cone snail and it has only 11 amino acid residues, SCSCKRNFLCC*, with the rare cysteine framework XVI and a monoisotopic mass of 1257.6Da. Two peptides were synthesized with two possible conformations: globular (pc16a_1) and ribbon (pc16a_2). pc16a_1 co-eluted with the native peptide, which indicates a disulfide connectivity I-III, II-IV. The structure of pc16a_1 was determined by NMR. Both synthetic peptides were used to elucidate the biological activity. Bioassays were performed on crickets, ghost shrimps, larvae of the mealworm beetle and mice, but no effect was seen. Using two-electrode voltage clamp, a range of voltage-gated ion channels (Na(v) and K(v)) and nicotinic acetylcholine receptors were screened, but again no activity was found. Hence, the specific target of pc16a still remains to be discovered.
journal_name
Peptidesjournal_title
Peptidesauthors
Van Der Haegen A,Peigneur S,Dyubankova N,Möller C,Marí F,Diego-García E,Naudé R,Lescrinier E,Herdewijn P,Tytgat Jdoi
10.1016/j.peptides.2011.10.026subject
Has Abstractpub_date
2012-03-01 00:00:00pages
106-13issue
1eissn
0196-9781issn
1873-5169pii
S0196-9781(11)00466-9journal_volume
34pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::High levels of neuropeptide Y (NPY) are found in the hypothalamus, median eminence, pituitary portal blood, and the pituitary in a number of species. Neuropeptide Y may influence the synthesis and secretion of a variety of hormones by interacting with specific receptors in the hypothalamus and/or the pituitary. To fur...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90090-6
更新日期:1994-01-01 00:00:00
abstract::Methods have been developed for the microsurgical interruption of nerve pathways in the wall of the intestine and for the immunohistochemical localization of antigens in whole mounts. These methods have made it possible to determine accurately the distributions and projections of neurons containing different putative ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(81)90022-x
更新日期:1981-01-01 00:00:00
abstract::Endocrine regulation in vertebrates is critical for the adaptation and regulation of homeostasis. The G protein-coupled receptor (GPCR) signaling transduction system represents one of the most ancient forms of cell surface signaling. Recently, comparative sequence analysis has aided in the identification and pairing o...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2004.05.021
更新日期:2004-10-01 00:00:00
abstract::Opiate alkaloids and opioid peptides have been shown to suppress plasma LH and FSH levels via a naloxone sensitive mechanism in several species including man. Three subtypes of opiate receptors have been characterized: mu, delta and kappa. The present study was designed to investigate their role in gonadotropin releas...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(83)90012-8
更新日期:1983-09-01 00:00:00
abstract::VIP was given intravenously over 1 min at the doses 0.1 and 0.2 micrograms X kg X min-1 to twenty-one anesthetized patients undergoing abdominal surgery. Intra-arterial blood pressure was monitored and various blood flows were measured simultaneously by electromagnetic technique. Following VIP, intra-arterial blood pr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(84)90227-4
更新日期:1984-03-01 00:00:00
abstract::The secretin receptor is a member of a large family of G-protein-coupled receptors that recognize polypeptide hormone and/or neuropeptides. Charged, conserved residues might play a key role in their function, either by interacting with the ligand or by stabilizing the receptor structure. Of the four charged amino acid...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00122-9
更新日期:1999-01-01 00:00:00
abstract::C-type natriuretic peptide (CNP) is expressed in the vascular endothelium. It is not known whether CNP is specifically increased in patients with idiopathic left ventricular systolic dysfunction (ILVDys) with or without overt heart failure, and whether in these patients it is related with indicators of myocardial and/...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.03.002
更新日期:2007-05-01 00:00:00
abstract::We examined the mechanism through which leptin increases Na(+), K(+)-ATPase activity in the rat kidney. Leptin was infused under anaesthesia into the abdominal aorta proximally to the renal arteries and then Na(+), K(+)-ATPase activity was measured in the renal cortex and medulla. Leptin (1mug/kgmin) increased Na(+), ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.08.010
更新日期:2006-12-01 00:00:00
abstract::A putative genome duplication event within the Silurana lineage has given rise to the tetraploid Cameroon clawed frog Silurana epitropicalis (Fischberg, Colombelli, and Picard, 1982). Peptidomic analysis of norepinephrine-stimulated skin secretions of S. epitropicalis led to identification of 10 peptides with varying ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.07.005
更新日期:2012-09-01 00:00:00
abstract::Prothymosin alpha (ProTα), a nuclear protein, plays multiple functions including cell survival. Most recently, we demonstrated that the active 30-amino acid peptide sequence/P30 (amino acids 49-78) in ProTα retains its substantial activity in neuroprotection in vitro and in vivo as well as in the inhibition of cerebra...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.02.022
更新日期:2013-05-01 00:00:00
abstract::We have previously described a 25mer anti-hypertensive peptide, previously named TsHpt-I (Tityus serrulatus Hypotensin-I), now Ts14, as an agonist of B2 kinin receptor. Bradykinin is known to play physiological roles in angiogenic, inflammatory, and fibrogenic processes, mostly mediated by B2 receptor. Therefore, we i...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2016.10.002
更新日期:2017-12-01 00:00:00
abstract::The experiments reported here investigate the conditionability, using taste aversion conditioning, of the antagonistic effects of alpha-MSH on the thermoregulatory changes associated with injection of bacterial endotoxin lipopolysaccharide (LPS) in rats. Animals were administered LPS and then given alpha-MSH, as an un...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90182-1
更新日期:1994-01-01 00:00:00
abstract::Injection of capsaicin into the hindpaw has been employed as a model of chemogenic nociception in mice. Intraplantar injection of nociceptin (30-240 pmol) produced a significant and dose-dependent antinociceptive activity in the capsaicin test. The nociceptin N-terminal fragments, (1-11) and (1-13), were also active w...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.05.022
更新日期:2005-12-01 00:00:00
abstract::Malaria lactate dehydrogenase, a glycolytic enzyme, is a malaria diagnostic target in lateral flow immunochromatographic rapid diagnostic tests. Recombinant Plasmodium yoelii LDH was cloned into the pET-28a vector, expressed and the expressed protein purified from a Ni-NTA affinity matrix. A pan-malarial LDH antibody ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.01.002
更新日期:2010-04-01 00:00:00
abstract::Motilin-immunopositive cells (Mo cells) are known to be present in the upper small intestine of various species, including man. However, whether Mo cells are present in the rabbit gastrointestinal tract remained to be elucidated. Therefore, this study was designed to investigate the distribution of Mo cells in the rab...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(95)00046-m
更新日期:1995-01-01 00:00:00
abstract::Obestatin regulates fluid and electrolyte homeostasis mainly by opposing the action of vasopressin (AVP). We measured plasma concentration of obestatin and AVP in patients with cardiorenal syndrome (CRS). Plasma AVP and obestatin concentration were measured in 34 patients with type II CRS. The data were compared to th...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.08.022
更新日期:2012-12-01 00:00:00
abstract::Enzyme-resistant receptor agonists of the incretin hormone glucagon-like peptide-1 (GLP-1) have shown positive therapeutic effects in people with type 2 diabetes mellitus (T2DM). T2DM has detrimental effects on brain function and impairment of cognition and memory formation has been described. One of the underlying me...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2017.11.017
更新日期:2018-02-01 00:00:00
abstract::The evolution, function and interaction of ligand-receptor pairs are of major pharmaceutical interest. Comparative sequence analysis approaches using data from phylogenetically distant organisms can provide insights into their origin and possible physiological roles. The present review focuses on the pituitary adenyla...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2007.05.016
更新日期:2007-09-01 00:00:00
abstract::Female Zucker lean and obese rats were treated for 14 days with 3.5 micromol/kg oleoyl-estrone (OE) in liposomes (Merlin-2). After 0, 3, 6, 10, and 14 days of treatment, the rats were killed and hypothalamic nuclei (lateral preoptic, median preoptic, paraventricular, ventromedial and arcuate) were used for neuropeptid...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(98)00104-1
更新日期:1998-01-01 00:00:00
abstract::The effect of synthetic human calcitonin gene-related peptide (hCGRP) on the isolated and electrically driven left atria of rats were investigated. The peptide at concentrations of 3 X 10(-9)-3 X 10(-7) M produced positive inotropic effects on the left atria in a dose-dependent manner. Verapamil (10(-5) M) and adenosi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90038-0
更新日期:1986-07-01 00:00:00
abstract::Peptide YY (PYY)(3-36), a neuropeptide Y (NPY) Y2 receptor agonist, is a powerful inhibitor of intestinal secretion. Based on this anti-secretory effect, NPY Y2 receptor agonists may be useful as novel anti-diarrheal agents, but anti-diarrheal efficacy has yet to be determined. We therefore examined the anti-diarrheal...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.11.005
更新日期:2010-04-01 00:00:00
abstract::Ceratotoxin A (CtxA), a 36-residue alpha-helical cationic peptide isolated from the medfly Ceratitis capitata, exhibits strong antibacterial activity. To determine its mode of action against bacteria, we investigated the behavior of ceratotoxin A by incorporating it into planar lipid bilayers. Macroscopic and single c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.09.015
更新日期:2003-11-01 00:00:00
abstract::Antimicrobial peptides (AMPs) are believed to be a promising class of antimicrobial agents against bacteria and fungi. To promote the clinical use of AMPs, their antimicrobial activity and susceptibility to protease degradation should be further improved. The antimicrobial peptide Jelleine-I was originally isolated fr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2018.11.006
更新日期:2019-02-01 00:00:00
abstract::Insulin has been detected by ELISA in the vitreous of the normal and streptozotocin-diabetic rat at levels for both about 1% of those in serum. 131I-labeled insulin, administered to conscious rats via an indwelling cannula in the right atrium, was found to cross the blood-ocular barrier into the vitreous. Autoradiogra...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90171-x
更新日期:1992-07-01 00:00:00
abstract::The vasoactive peptide urotensin-II (U-II) is best known for its ability to regulate peripheral vascular and cardiac contractile function in vivo, and recent in vitro studies have suggested a role for the peptide in the control of vascular remodeling by inducing smooth muscle proliferation and fibroblast-mediated coll...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.05.028
更新日期:2005-12-01 00:00:00
abstract::Neurotensin-like immunoreactive neuronal perikarya, fibers and terminals in in the rat hypothalamus were investigated by light and electron microscopic immunocytochemistry. Distributional density and pattern of these elements were clarified. Fine structure of immunoreactive neuronal perikarya with respect to developme...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(84)90270-5
更新日期:1984-01-01 00:00:00
abstract::Numerous works associate the MCH peptide, and the hypothalamic neurons that produce it, to the feeding behavior and energy homeostasis. It is commonly admitted that MCH is an orexigenic peptide, and MCH neurons could be under the control of arcuate NPY and POMC neurons. However, the literature data is not always conco...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2009.07.008
更新日期:2009-11-01 00:00:00
abstract::Intracerebroventricular (ICV) and subcutaneous (SC) injections of sauvagine powerfully inhibited gastric acid secretion stimulated by gastric distension and by 2-deoxy-D-glucose, but not by histamine in pylorus-ligated rats. Naloxone failed to antagonize the antisecretory effects of SC and ICV sauvagine. Intravenous i...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90131-3
更新日期:1988-07-01 00:00:00
abstract::Corticotropin releasing hormone (CRH) stimulates pituitary ACTH secretion through type-1 CRH (CRH1) receptors. Stimulation of the hypothalamic pituitary adrenal (HPA) axis as well as increased corticotroph responsiveness during stress and adrenalectomy are associated with marked pituitary CRH binding downregulation. T...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(01)00390-4
更新日期:2001-05-01 00:00:00
abstract::Recent studies have provided evidence for the dense localization of atrial natriuretic factor (ANF) in the anteroventral third ventricle (AV3V) region of the rat brain. This area is currently thought to be involved in the regulation of blood pressure and fluid and electrolyte balance. To investigate whether ANF may pl...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90425-5
更新日期:1985-11-01 00:00:00