Abstract：
:Alginate hydrogels have been used widely in tissue engineering for cell encapsulation for several reasons: low toxicity, the ability to gel under gentle condition and compatibility with cells. In this study, we determined the effect of different concentrations of alginate on encapsulation of 3T3 fibroblast cells at two different cell seeding densities. Live/dead staining and MTT assay were performed at regular intervals up to 4 weeks. A Hoechst 33258 assay was done to validate the MTT results. There were more dead cells on day 1 for the higher concentrations of alginate while at, the lower concentration of alginate, cell proliferation and spheroid formation occurred more quickly. Furthermore, at low cell seeding density, cell proliferation was prolonged compared to the intermediate seeding density. In conclusion, by altering both alginate concentration and cell seeding density, proliferation and spheroid formation can be controlled.
journal_name
Biomed Mater Engjournal_title
Bio-medical materials and engineeringauthors
Bohari SP,Hukins DW,Grover LMdoi
10.3233/BME-2011-0665subject
Has Abstractpub_date
2011-01-01 00:00:00pages
159-70issue
3eissn
0959-2989issn
1878-3619pii
3835113702140L35journal_volume
21pub_type
杂志文章abstract：:Sunscreens that absorb UV light without photodegradation could reduce skin cancer. Polyvinyl silsesquioxanes are known to have greater thermal and photochemical stability than organic compounds, such as those in sunscreens. This paper evaluates the UV transparency of vinyl silsesquioxanes (VS) and its hybrids with SiO...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-130853
更新日期：2014-01-01 00:00:00
abstract：:Longitudinal stent compression (LSC) is a new failure mode not previously observed in coronary stents. This phenomenon occurs when the physician tries to cross the deployed stent with other devices. While this phenomenon has been observed with a number of stent designs, it seems more common with the Element stent. A c...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-130781
更新日期：2014-01-01 00:00:00
abstract：:A novel technique and product for carpal tunnel microscopic surgical procedures using a modified medical device containing a head as a surgical scalpel under light guidance were designed and studied. The novel design of the medical device was developed and applied in a new carpal tunnel microscopic surgical procedure ...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-151303
更新日期：2015-01-01 00:00:00
abstract：:This study investigates the radiation safety of a newly designed magnetic induction sensor. This novel magnetic induction sensor uses a two-arm Archimedean spiral coil (TAASC) as the exciter. A human head model with a real anatomical structure was used to calculate the specific absorption rate (SAR) and temperature ch...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-151329
更新日期：2015-01-01 00:00:00
abstract：:Shape memory alloys (SMA) are being increasingly used in various industrial applications as actuators, connectors, or damping materials. In the medical field, superelastic devices such as eyeglass frames, stents or guide catheters have come to market in the recent years. The design of SMA devices has usually been base...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Poly(propylene fumarate-co-ethylene glycol) random (PPF-1) and block (PPF-2) copolymer oligomers were prepared. Comparing the setting characteristics of PPF-1 and PPF-2 with comonomer n-vinyl pyrrolidone (n-VP) and swelling characteristics of cured PPF-1 and PPF-2, lower setting temperature and setting time was observ...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The aim of the present study was to determine the degradability of aliphatic polyurethanes, based on a different amount of synthetic, atactic poly[(R,S)-3-hydroxybutyrate] (a-PHB), in hydrolytic (phosphate buffer) and oxidative (H2O2/CoCl2) solutions. The soft segments were built with atactic poly[(R,S)-3-hydroxybutyr...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-151262
更新日期：2015-01-01 00:00:00
abstract：:In this work, we present an evolutive trabecular model for bone remodeling based on a boundary detection algorithm accounting for both biology and applied mechanical forces, known to be an important factor in bone evolution. A finite element (FE) numerical model using the Abaqus/Standard® software was used with a UMAT...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-171636
更新日期：2017-01-01 00:00:00
abstract：:The effect of carbonate in reducing the crystal size of precipitated hydroxyapatite by approximately an order of magnitude has not been used previously in the preparation of gel monoliths for the fabrication of carbonate hydroxyapatite ceramics. The aim of this study was to devise a method whereby gel monoliths of car...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:In this investigation, ultrasonic parameters of kidney stones are measured, in vitro, using a double probe ultrasonic-through-transmission technique. Due to the complex chemical structure of these stones, a large variation in the values of the ultrasonic parameters is found. A correlation between ultrasonic properties...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:The cilia, presenting a rotational movement in the embryonic nodes, play a crucial role in the left-right specification during embryogenesis. The characteristic architecture of these cilia is based on a cylindrical arrangement of 9 doublet microtubules and the motion of the cilia is triggered by the dynein motors loca...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-141063
更新日期：2014-01-01 00:00:00
abstract：:Hydroxyapatite has been rubbed against ultra-high-molecular-weight-polyethylene (UHMWPE) under calcium-containg aqueous solutions. Further, hardness tests were carried out in air and in calcium-containing solutions whose pH ranged from pH 5 to pH 9. Hardness was found to vary with pH with a peak at around pH 7, i.e. -...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Accelerated hydrothermal aging has long been one of the most widely accepted quality control tests for simulating low-temperature degradation (LTD) in zirconia-containing implants used in total hip arthroplasty (THA). However, it is still unclear how much consistency there is between the experimental predict...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-201083
更新日期：2020-01-01 00:00:00
abstract：:Food systems are complex and inhomogeneous in nature. Ultrasonic propagation velocity in the food systems, in this case processed milk cream and vegetable oils, is studied. Ultrasonic properties of the food systems are similar to those of complex biological tissues. The presence of vegetable oil as an adulterant in th...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:The ion selective transport plays an important role in the function of cell membranes, and promotes the application of artificial permeable membranes. This phenomenon has been studied in case for different diameters and functional groups of nanopores. In this work, we focus on the effect of anions on cation selectivit...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-130822
更新日期：2014-01-01 00:00:00
abstract：INTRODUCTION:Repairing contour defects is a challenge in plastic surgery. Different filling materials have been used with inadequate results and complications. The autologous fat transfer is the standard technique at the moment, but adipose tissue reserves are limited. The aim of our study was to compare in vivo on an ...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:To evaluate the ability of assessing the refractive change induced by myopic laser surgery using the intrinsic data, obtained from a reference intrinsic to the cornea. Twenty-nine eyes with laser in situ keratomileusis for myopia were included in the study. Statistical analyses were performed to determine the relation...
journal_title：Bio-medical materials and engineering
pub_type： 临床试验,杂志文章
doi：10.3233/BME-151374
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:In order to manufacture pharmaceutical products, real-time monitoring in the manufacturing process is necessary, but large equipment cost is required to achieve it. OBJECTIVE:The aim of this research is to use ultra-violet-visible spectroscopy along with chemometrics procedure to simultaneously carry out qu...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-181034
更新日期：2019-01-01 00:00:00
abstract：:Based upon previous research on the relation between hip prosthesis designs and strain distributions in a proximal femur model, a prototype of a "physiological" hip prosthesis was designed and manufactured. Strain gauge measurements on a dry femur before and after implantation of this prosthesis were made in different...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:The 5(and 6)-carboxyfluorescein diacetate (C-FDA) is used for a rapid and continuous counting of living cells. Nonfluorescent C-FDA is converted into fluorescent 5(and 6)-carboxyfluorescein (C-F) by the reaction with esterase, which is an enzyme of living cells. The conversion makes it possible to count the number of ...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:We previously reported a simple method for the preparation of size-controlled spherical silver nanoparticles (Ag NPs) generated by autoclaving a mixture of silver-containing glass powder and glucose. The particle size is regulated by the glucose concentration, with concentrations of 0.25, 1.0 and 4.0 wt% glucose provi...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-130772
更新日期：2013-01-01 00:00:00
abstract：:The values of the propagation coefficient measured on a silicon rubber tube in the frequency range 1-15 Hz were compared, using four different equations. The first formula is based on three simultaneous pressure measurements performed at equidistant points; the remaining three equations are original, and make use of o...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:The interaction between implant materials and bone cells or oral epithelial (OE) cells contributes to the clinical success of dental implants. The functional activity of cells in contact with an implant is determined by its surface properties. Before cells attach, extracellular matrix (ECM) in the serum deposits on th...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：:To address the lack of 3D space information in the digital radiography of a patient femur, a pose estimation method based on 2D-3D rigid registration is proposed in this study. The method uses two digital radiography images to realize the preoperative 3D visualization of a fractured femur. Compared with the pure Digit...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-141103
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Great potential of cellular therapies has generated extensive research in the field of cells harvesting and culturing. Transplantation of cell cultures has been used in a variety of therapeutic programs but in many cases it appeared that biomaterial scaffold or sheath would enhance cells regenerative potenti...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-140966
更新日期：2014-01-01 00:00:00
abstract：:The present review surveys studies on physical-chemical properties and biological response of living tissues to NiTi (Nitinol) carried out recently, aiming at an understanding of the place of this material among the implant alloys in use. Advantages of shape memory and superelasticity are analyzed in respect to functi...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:The balance between extracellular inorganic phosphate (ePi) and extracellular inorganic pyrophosphate (ePPi) is controlled by four membrane proteins: the transporters ANK (exporting PPi outside the cells) and PiT-1 (importing ePi into the cells), and the enzymes PC-1 (generating ePPi from nucleotides) and Tissue Non-s...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-2010-0634
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:To study the comprehensive biomechanical properties of the cancellous bone of distal femur through a series of mechanical tests, and provide relevant subjects with the basic technical data. BACKGROUND:The study on bone mechanics is a commonly used approach to evaluate the biomechanical competency of bone. Th...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:The selection of best material pair in the hip prosthetics design for improved performance and life relies on the estimation of hip joint contact stresses and contact pressure distribution during various dynamic loading cycles: Climbing Upstairs, Climbing downstairs and Knee bending. The maximum Von Mises stress, cont...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-181019
更新日期：2018-01-01 00:00:00
abstract：:In this study, design optimization of a tooth implant-supported fixed prosthesis was investigated theoretically. A three-dimensional finite element analysis was utilized to simulate the stress distribution and deformation, with an emphasis on the material selection for various parts of the prosthesis. This mandibular ...
journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
