Abstract:
:The 5(and 6)-carboxyfluorescein diacetate (C-FDA) is used for a rapid and continuous counting of living cells. Nonfluorescent C-FDA is converted into fluorescent 5(and 6)-carboxyfluorescein (C-F) by the reaction with esterase, which is an enzyme of living cells. The conversion makes it possible to count the number of living cells by detecting fluorescence. Experimental results show that the living cells of beer's yeast, E. coli, and Lactobacillus bulgaricus can be detected by fluorescence. The flow cell system was used to experiment a continuous detection of living cells, so that the number of living cells of beer's yeast can be detected continuously. The proposed method can be applied to the on-line counting in the food plants.
journal_name
Biomed Mater Engjournal_title
Bio-medical materials and engineeringauthors
Sugata K,Ohnishi T,Matsumoto Ksubject
Has Abstractpub_date
1991-01-01 00:00:00pages
115-25issue
2eissn
0959-2989issn
1878-3619journal_volume
1pub_type
杂志文章abstract::A layer of L-glycine-molecule-imprinted polyaniline (LMIP-PANI) polymer film has been modified on a carbon fiber electrode for the determination of L-glycine standard samples and L-glycine in cerebrospinal fluid of wistar mice. It has been found that a linear relationship exists between current and concentration for t...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130907
更新日期:2014-01-01 00:00:00
abstract::Two surface analytical spectroscopic techniques (namely, angular-dependent X-ray photoelectron spectroscopy and static secondary ion mass spectrometry) were used to characterize the nature and composition of the surface and near-surface layers formed on three biomedical polymers (namely, low-density polyethylene, expa...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::In this study, biocompatibility of 17-4 PH stainless steel foam for biomedical implant applications was investigated. 17-4 PH stainless steel foams having porosities in the range of 40-82% with an average pore size of around 600 μm were produced by space holder-sintering technique. Sintered foams were precipitation ha...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-2011-0671
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Retearing mechanism after transosseous equivalent (TOE) rotator cuff repair has not been fully clarified yet. OBJECTIVE:The purposes of this study were to compare the stress distribution pattern in the tendon stump between knotted and knotless TOE repair and to investigate the role of suture tension applied...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-171673
更新日期:2017-01-01 00:00:00
abstract::Based upon previous research on the relation between hip prosthesis designs and strain distributions in a proximal femur model, a prototype of a "physiological" hip prosthesis was designed and manufactured. Strain gauge measurements on a dry femur before and after implantation of this prosthesis were made in different...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract:BACKGROUND:Cuff electrodes have been widely used chronically in different clinical applications. Advancements have been made in selective stimulation by using multi-contact cuff electrodes. Steering anodic current is a strategy to increase selectivity by reshaping and localizing electric fields. There are two configura...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-151281
更新日期:2015-01-01 00:00:00
abstract::The purpose of this study is to compare the differences in flexural strength and compressive strength between four resin-modified luting glass ionomer cements that are commonly used in clinics. Furthermore, this study investigates the influence of curing mode on the flexural strength and compressive strength of dual-c...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-151284
更新日期:2015-01-01 00:00:00
abstract::The aim of the present study was to determine the degradability of aliphatic polyurethanes, based on a different amount of synthetic, atactic poly[(R,S)-3-hydroxybutyrate] (a-PHB), in hydrolytic (phosphate buffer) and oxidative (H2O2/CoCl2) solutions. The soft segments were built with atactic poly[(R,S)-3-hydroxybutyr...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-151262
更新日期:2015-01-01 00:00:00
abstract::Modeling of thermal-induced mechanical behaviors of soft tissues is of great importance for thermal ablation. This paper presents a method by integrating the heating process with thermal-induced mechanical deformations of soft tissues for simulation and analysis of the thermal ablation process. This method combines bi...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141043
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:Chondrocytes hypertrophy is a physiological process observed in endochondral ossification during development until adolescence in human. It can also be observed during pathophysiological conditions such as osteoarthritis. Hypertrophic chondrocytes synthesise collagen X and express matrix metalloproteinase 13...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141252
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Adipose tissue engineering has been studied as an alternative to current options for breast reconstruction, such as lipofilling, flap reconstruction, and silicone implants. Previously, we demonstrated that a poly(L-lactic acid) mesh containing a collagen sponge, containing neither cells nor growth factors, c...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-201103
更新日期:2020-01-01 00:00:00
abstract::Since adhesive technology was introduced into dental field, metal-based restoration has been gradually replaced by metal-free restoration. Using the adhesive technology, minimum invasive technique has been possible in daily clinical practice as well as esthetic tooth-colored restorations have become very popular all o...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章,评审
doi:10.3233/BME-2009-0587
更新日期:2009-01-01 00:00:00
abstract::The objective of the present study was to find a standard substance for use as a reference in the cytotoxicity assay of biomaterials, as an alternative to animal experiments in recent years. Eight kinds of rubber were made in a plate shape to keep their surface area at 1 cm2 against 10 ml of extract volume. They were ...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::The values of the propagation coefficient measured on a silicon rubber tube in the frequency range 1-15 Hz were compared, using four different equations. The first formula is based on three simultaneous pressure measurements performed at equidistant points; the remaining three equations are original, and make use of o...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Skilled behavior is difficult or impossible to articulate explicitly by the performers. Likewise biomechanical models of skilled motor actions are often limited by the lack of knowledge of the underlying mechanisms. A 'behavioral cloning' technique is described, based on a trained artificial neural network (ANN), that...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::To evaluate the influence of Zn on the formation of hydroxyapatite (HA), zinc-containing glass 58S4Z was prepared by substitution of CaO with 4 wt% ZnO in 58S bioactive glass, and Zn-containing simulated body fluid (ZnSBF) was prepared as soaking fluid. During the soaking, all samples could induce the deposition of HA...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Scallop shell powder is called bioshell calcium oxide (BiSCaO), which is known to possess deodorizing properties and broad antimicrobial activity against various pathogenic microbes, including viruses, bacteria, spores, and fungi. OBJECTIVE:This study aims to investigate the applications of BiSCaO suspensio...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-201082
更新日期:2020-01-01 00:00:00
abstract::The fretting corrosion of five materials for implant suprastructures (cast-titanium, machined-titanium, gold alloy, silver-palladium alloy and chromium-nickel alloy), was investigated in vitro, the materials being galvanically coupled to a titanium ball-and-socket-joint with tetrafluoroethylene under mechanical load. ...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::To evaluate the ability of assessing the refractive change induced by myopic laser surgery using the intrinsic data, obtained from a reference intrinsic to the cornea. Twenty-nine eyes with laser in situ keratomileusis for myopia were included in the study. Statistical analyses were performed to determine the relation...
journal_title:Bio-medical materials and engineering
pub_type: 临床试验,杂志文章
doi:10.3233/BME-151374
更新日期:2015-01-01 00:00:00
abstract::In order to investigate the significance of hydroxyapatite based microporous composite (HA/mica composite) surfaces and a macroporous synthetic hydroxyapatite, rat marrow cell culture, which shows osteogenic differentiation, was carried out on six different culture substrata (two control culture dishes, two identical ...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The balance between extracellular inorganic phosphate (ePi) and extracellular inorganic pyrophosphate (ePPi) is controlled by four membrane proteins: the transporters ANK (exporting PPi outside the cells) and PiT-1 (importing ePi into the cells), and the enzymes PC-1 (generating ePPi from nucleotides) and Tissue Non-s...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-2010-0634
更新日期:2010-01-01 00:00:00
abstract::To address the lack of 3D space information in the digital radiography of a patient femur, a pose estimation method based on 2D-3D rigid registration is proposed in this study. The method uses two digital radiography images to realize the preoperative 3D visualization of a fractured femur. Compared with the pure Digit...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141103
更新日期:2014-01-01 00:00:00
abstract::Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of ...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-140978
更新日期:2014-01-01 00:00:00
abstract::A double-layered tube consisting of a porous inner tube and a solid outer tube was used to perfuse isotonic saline solution into blood to prevent blood cell adhesion. Polystyrene/poly(styrene-co-butadiene) (PS-SBR) porous tubes were made using a dipping method. Citrated canine blood was circulated for 30 min with the ...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Chitosan oligosaccharides graft polycaprolactone copolymer (PHCSO-g-PCL) has been synthesized via initiating the polymerization of e-caprolactone (CL) monomer through an amino group protection route using phthaloyl chitosan oligosaccharide (PHCSO) as intermediate. The grafting reaction was carried out in Pyridine at 1...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141013
更新日期:2014-01-01 00:00:00
abstract::Significant progress has been achieved in the field of pH-sensitive drug delivery system in recent years. For layer-by-layer self-assembly nanocarriers, various pH values can induce the change of permeability and structural stability of the outer polyelectrolyte multi-layers, resulting in the release of the loaded dru...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141033
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:In order to manufacture pharmaceutical products, real-time monitoring in the manufacturing process is necessary, but large equipment cost is required to achieve it. OBJECTIVE:The aim of this research is to use ultra-violet-visible spectroscopy along with chemometrics procedure to simultaneously carry out qu...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-181034
更新日期:2019-01-01 00:00:00
abstract::Sino-atrial node cells (SANCs) play a significant role in rhythmic firing. To investigate their role in arrhythmia and interactions with the atrium, computer simulations based on cellular dynamic mathematical models are generally used. However, the large-scale computation usually makes research difficult, given the li...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-151365
更新日期:2015-01-01 00:00:00
abstract::We previously reported a simple method for the preparation of size-controlled spherical silver nanoparticles (Ag NPs) generated by autoclaving a mixture of silver-containing glass powder and glucose. The particle size is regulated by the glucose concentration, with concentrations of 0.25, 1.0 and 4.0 wt% glucose provi...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130772
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Introduction of a new generation of artificial ligaments for ACL reconstruction, the Ligament Augmentation and Reconstruction System (LARS), gives promising clinical results [1]. The current literature supports the use of LARS from short to medium term. To go even further to improve the biocompatibility of t...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130753
更新日期:2013-01-01 00:00:00