Abstract：
:Short cell penetrating peptides (CPP) are widely used in vitro to transduce agents into cells. But their systemic effect has not been yet studied in detail. We studied the systemic effect of the cell penetrating peptides, penetratin, transportan and pro-rich, on rat hemodynamic functions. Intra-arterial monitoring of blood pressure showed that injection of the positively charged penetratin and transportan in a wide range of concentrations (2.5-320 μg/kg) caused highly significant transient decrease in the systolic and diastolic blood pressure in a dose dependent manner (p<0.01). Pretreatment with histamine receptors blockers or with cromolyn, a mast cell stabilizing agent, significantly attenuated this effect. Furthermore, in vitro incubation of these both peptides with mast cells line, LAD2, caused a massive mast cell degranulation. In vitro studies showed that these CPP in a wide range of concentrations were not cytotoxic without any effect on the survival of LAD2 mast cell line. In contrast, the less positively charged and proline-rich CPP, pro-rich, had no systemic effects with no effect on mast cell degranulation. Our results indicate that intravenously administrated positively charged CPP may have deleterious consequences due to their induced BP drop, mediated by mast cell activation. Therefore, the major effect of mast cell activation on BP should be considered in developing possible future drug therapies based on the injection of membrane-permeable and positively charged CPP. Nevertheless, lower levels of such CPP may be considered as a treatment of systemic high BP through moderate systemic mast cell activation.
journal_name
Peptidesjournal_title
Peptidesauthors
Basheer M,Schwalb H,Shefler I,Levdansky L,Mekori YA,Gorodetsky Rdoi
10.1016/j.peptides.2011.09.017subject
Has Abstractpub_date
2011-12-01 00:00:00pages
2444-51issue
12eissn
0196-9781issn
1873-5169pii
S0196-9781(11)00383-4journal_volume
32pub_type
杂志文章相关文献
PEPTIDES文献大全abstract：:Milk is one of the main source of biologically-active peptides that may function as regulatory substances called food hormones. After passing the gut-blood barrier, the μ-opioid receptor agonist and antagonist peptides may become the new factors influencing various functions of the human organism. The aim of the condu...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2010.12.003
更新日期：2011-04-01 00:00:00
abstract：:Neuromedin B (NMB), which was originally isolated from porcine spinal cord, is a mammalian bombesin-related peptide that exerts various physiological effects. Previously, we observed expression of NMB in rib cartilage from chicken. Here, we report the initial attempt to elucidate the role of NMB in cartilage. We used ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.05.010
更新日期：2012-08-01 00:00:00
abstract：:In this study we examined the effects of 2% saline loading (S), partial restriction of water consumption (R) or a combination of S or R with dexamethasone (DEX) treatment for 14 days on corticotropin releasing factor (CRF)-41 content of the neurointermediate lobe (NIL) and supraoptic nuclei (SON) of male Wistar rats. ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(90)90109-i
更新日期：1990-01-01 00:00:00
abstract：:Leptin, a hormone secreted by the adipocytes and involved in feeding and energy balance control, has been proposed to modulate alcohol craving in mice and humans. This study evaluated whether leptin modulates alcohol intake in Marchigian Sardinian alcohol-preferring (msP) rats. Rats were offered 10% ethanol either 2h ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2003.09.008
更新日期：2003-09-01 00:00:00
abstract：:Intracerebroventricular (ICV) and subcutaneous (SC) injections of sauvagine powerfully inhibited gastric acid secretion stimulated by gastric distension and by 2-deoxy-D-glucose, but not by histamine in pylorus-ligated rats. Naloxone failed to antagonize the antisecretory effects of SC and ICV sauvagine. Intravenous i...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90131-3
更新日期：1988-07-01 00:00:00
abstract：:Spasmolytic polypeptide (SP) and intestinal trefoil factor (ITF) are trefoil peptides expressed by gut mucus cells. Using specific antisera we have quantified and characterized the molecular forms and distribution of these peptides in the rat gut. SP predominates in the gastric antrum as a 12 kDa form. ITF (7 kDa) is ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(95)00070-z
更新日期：1995-01-01 00:00:00
abstract：:Besides its well known endocrinological effects, thyrotropin-releasing hormone (TRH) has potential clinical value in the treatment of neurotrauma and various neurologic and psychiatric disorders. The aim of this study was to assess if transdermal delivery of TRH and its analogue, M-TRH, in the presence of enhancers, i...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(00)00358-2
更新日期：2001-01-01 00:00:00
abstract：:Two peptides, with a molecular mass of about 11 kDa and an N-terminal sequence abundant in arginine and glutamine residues, were isolated from wax gourd seeds. The isolation protocol included affinity chromatography on Affi-gel blue gel, ion-exchange chromatography on Mono-S and gel filtration on Superdex 75. The pept...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(02)00271-1
更新日期：2003-01-01 00:00:00
abstract：:We screened nearly 1000 synthetic peptides and found that JAL-AK22 (KYEGHWYPEKPYKGSGFRCIHI), which is derived from the BoxA domain in the Tob1 protein, activates both unfolded and folded proMMP-7. Interestingly, the smaller derivative of JAL-AK22, termed JAL-TA9 (YKGSGFRMI) possessed auto-proteolytic activity and clea...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2019.03.005
更新日期：2019-06-01 00:00:00
abstract：:Neurotensin exerts its actions in the central nervous system and the periphery through three identified receptors. Two of them, the NTS2 and NTS3, display unusual properties either because of their complex signal transduction mechanisms (NTS2) or because of their structural composition as a non-G-protein-coupled recep...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2006.04.026
更新日期：2006-10-01 00:00:00
abstract：:Peptides are an important class of molecules with diverse biological activities. Many endogenous peptides, especially neuropeptides and peptide hormones, play critical roles in development and regulating homeostasis. Furthermore, as drug candidates their high receptor selectivity and potent binding leads to reduced of...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2020.170369
更新日期：2020-09-01 00:00:00
abstract：:Suppression subtractive hybridization comparing brains from diapausing and nondiapausing pupae of the flesh fly, Sarcophaga crassipalpis, suggested that the gene encoding neuropeptide-like precursor 4 (Nplp4) was uniquely expressed during diapause. We have sequenced the full-length cDNA encoding Nplp4 and used Norther...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2008.10.008
更新日期：2009-03-01 00:00:00
abstract：:CR 1409, a glutaramic acid derivative with competitive cholecystokinin-antagonistic activity, was administered IP and evaluated in comparison with proglumide (the model CCK-receptor antagonist), gabexate (protease inhibitor) and PGE2 (cytoprotective) on two different models of experimental pancreatitis. Acute pancreat...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(86)90147-6
更新日期：1986-11-01 00:00:00
abstract：:We investigated the effect of synthetic antimicrobial decapeptide KSL-W (KKVVFWVKFK) on normal human gingival fibroblast growth, migration, collagen gel contraction, and α-smooth muscle actin protein expression. Results show that in addition to promoting fibroblast adhesion by increasing F-actin production, peptide KS...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2017.05.003
更新日期：2017-07-01 00:00:00
abstract：:Receptors for neuropeptide Y (NPY) and peptide YY (PYY) have been extensively characterized in the brain. Less is known about NPY receptor subtypes in the spleen, though it is well established that NPY produces vascular contraction in this tissue. In the present study, we found an unusually high density of Y1 receptor...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(96)00228-8
更新日期：1996-01-01 00:00:00
abstract：:The cell adhesion molecule, N-cadherin plays a pivotal role in many biological and disease processes. Drugs that modulate N-cadherin function should therefore be useful therapeutic agents. We have used phage display technology to identify amino acid sequences capable of binding to N-cadherin. All of these sequences ha...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2008.06.025
更新日期：2008-11-01 00:00:00
abstract：:Enzyme-resistant receptor agonists of the incretin hormone glucagon-like peptide-1 (GLP-1) have shown positive therapeutic effects in people with type 2 diabetes mellitus (T2DM). T2DM has detrimental effects on brain function and impairment of cognition and memory formation has been described. One of the underlying me...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2017.11.017
更新日期：2018-02-01 00:00:00
abstract：:Higher plasma copeptin level has been associated with poor outcomes of critical illness. The present study was undertaken to investigate the plasma copeptin concentrations in children with traumatic brain injury (TBI) and to analyze the correlation of copeptin with disease outcome. Plasma copeptin concentrations of 12...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2013.01.015
更新日期：2013-04-01 00:00:00
abstract：:The biosynthetic activity of rat intermediate lobe melanotropes in vivo is inhibited by stimulation of dopamine D2 receptors. Individual melanotropes are innervated differentially by dopaminergic axons and vary in their levels of pro-opiomelanocortin (POMC) mRNA. We tested the hypothesis that placement of the lobe in ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(98)00008-4
更新日期：1998-01-01 00:00:00
abstract：:We have developed a specific and sensitive radioimmunoassay for the hydra head activator neuropeptide (HHAP). The antibody recognizes the C-terminal portion of HHAP and shows no cross-reactivity with other neuropeptides. The assay allows measurement of HHAP-like material in tissue extracts with a minimum detectable co...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(90)90081-f
更新日期：1990-03-01 00:00:00
abstract：:The incretin hormones, glucose dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP-1), potentiate insulin secretion and are responsible for the majority of insulin secretion that occurs after a meal. They may also, however, have a fundamental role in pancreatic beta cell development and functio...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2016.03.012
更新日期：2016-05-01 00:00:00
abstract：:An antifungal peptide with a molecular mass of 9 kDa was isolated from fresh fruiting bodies of the mushroom Agrocybe cylindracea. The isolation procedure comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatogr...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2004.09.011
更新日期：2005-02-01 00:00:00
abstract：:The influence of peripheral somatostatin immunoneutralization on intravenous (i.v.) and intracerebroventricular (ICV) bombesin-induced inhibition of gastric acid secretion (GAS) was investigated with the somatostatin monoclonal antibody, CURE.S6, in rats. The somatostatin antibody, injected i.v. in conscious rats with...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(94)00152-v
更新日期：1995-01-01 00:00:00
abstract：:alpha-MSH stimulates steroid secretion by rat adrenal zona glomerulosa cells and tissues but not fasciculata/reticularis cells when added to in vitro incubations. With glomerulosa cells from control animals on a normal sodium intake, production of corticosterone was significantly stimulated at an alpha-MSH concentrati...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(81)80026-5
更新日期：1981-07-01 00:00:00
abstract：:Neuropeptide Y (NPY) is one of the most potent stimulants of food intake in mammals, but very little is known about NPY actions in fish. The present study investigated the role of NPY in food intake in the rainbow trout (Oncorhynchus mykiss). Food intake was monitored at different times after intracerebroventricular a...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2005.09.014
更新日期：2006-04-01 00:00:00
abstract：:A pancreatic ductal carcinoma was established as a transplantable tumor line in an inbred strain of Syrian golden hamsters. Intracellular recordings of membrane potentials and input resistance were made from cultured cells obtained from the transplanted tumors using indwelling glass microelectrode. The mean value of t...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(84)90247-x
更新日期：1984-03-01 00:00:00
abstract：:By using specific antibodies and radioimmunological and immunohistochemical methods, we here show that neuropeptide FF (NPFF) occurs in cervical and lumbar dorsal root ganglia cells. Levels in the ganglia were low because they were detectable only after colchicine treatment or after unilateral dorsal rhizotomy. Simila...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(99)00039-x
更新日期：1999-01-01 00:00:00
abstract：:Our previous findings that the degree of endotoxin-induced hypotension in the dog is inversely related to ambient temperature (19 degrees through 30 degrees C) and that only increased doses of naloxone are effective at 19 degrees C suggested that opioid activity is also influenced by ambient temperature, increasing in...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(85)90007-5
更新日期：1985-01-01 00:00:00
abstract：:The binding of monoiodo 125I-Trp11-neurotensin to purified rat gastric fundus smooth muscle plasma membranes was characterized. Specific binding of ligand in subcellular fractions from rat fundus smooth muscle showed a distribution that paralleled that of several plasma membrane marker enzymes. 125I-Trp11-neurotensin ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(84)90117-7
更新日期：1984-09-01 00:00:00
abstract：:The in vivo effects of JMV-320 (a highly selective CCKB receptor ligand) and of CCK-4 on exploratory activity and memory in rats were compared. JMV-320 and CCK-4 did not modulate exploratory activity in an open field test but decreased it in an elevated plus-maze. CCK-4 (50 micrograms/kg) impaired passive avoidance re...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(94)00214-q
更新日期：1995-01-01 00:00:00
