当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Expression and characterization of the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II using the baculovirus expression system.

Expression and characterization of the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II using the baculovirus expression system.

Abstract：

:Sf9 cells infected with the recombinant mouse CaMKII-alpha (Ca2+/calmodulin dependent kinase II) baculovirus expressed 12-15 mg of MCaMKII-alpha per liter of cells. Approximately 50% of the MCaMKII-alpha activity could be purified using a CaM-Sepharose affinity column. The purified MCaMKII-alpha had a M(rapp) of 50 kDa by SDS-PAGE and a native Mr of 600 kDa. MCaMKII-alpha, like rat brain CaMKII, had an A0.5 for CaM of 100 nM, a Km for syntide-2 of 8 microM, and was able to generate Ca2(+)-independent activity by autophosphorylation. The baculovirus system expressed large quantities of MCaMKII-alpha with characteristics similar to the rat brain CaMKII, thus providing an expression system for the detailed biochemical analysis of MCaMKII-alpha.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Brickey DA,Colbran RJ,Fong YL,Soderling TR

doi

10.1016/s0006-291x(05)80074-9

subject

Has Abstract

pub_date

1990-12-14 00:00:00

pages

578-84

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)80074-9

journal_volume

173

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • An Australian halobacterium contains a novel proton pump retinal protein: archaerhodopsin.

    abstract：:A bacterial strain collected from Western Australia carries all the specific features of Halobacteria, an extremely halophilic archaebacteria. This strain, Halobacterium sp. aus-1, contains a retinal protein which does differ from bacteriorhodopsin but still pumps out protons in the light. This novel proton pump is na...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80509-6

    authors： Mukohata Y,Sugiyama Y,Ihara K,Yoshida M

    更新日期：1988-03-30 00:00:00

  • The induction of cyclo-oxygenase-2 in human pulmonary epithelial cell culture (A549) activated by IL-1beta is inhibited by tyrosine kinase inhibitors.

    abstract：:Cyclo-oxygenase (COX) exists as two isoforms. In endothelial cells, the induction of COX (COX-2) elicited by endotoxin or inflammatory cytokines is mediated by tyrosine kinase. Here we have investigated whether the induction of COX-2 elicited by IL-1beta in human pulmonary epithelial cells (A549) is mediated by tyrosi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0377

    authors： Akarasereenont P,Thiemermann C

    更新日期：1996-03-07 00:00:00

  • Phosphorylation of the band 4.1-like proteins of the bovine lens.

    abstract：:Crude membrane fractions prepared from the superficial cortical fiber cells of bovine lenses were incubated with [gamma-32P]-ATP. Membrane-associated 4.1-like proteins were then immunoprecipitated and analyzed for 32P-incorporation. Band 4.1-like proteins of 150 kd, 80 kd, and 78 kd were found to be labeled in a time-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90308-0

    authors： Aster JC,Brinn K,Maisel H,Brewer GJ

    更新日期：1985-02-15 00:00:00

  • Activation of nit-1 nitrate reductase by W-formate dehydrogenase.

    abstract：:Formate dehydrogenase ( FDH ) from Clostridium thermoaceticum is a known tungsten enzyme. FDH was tested for the presence of nitrogenase-type cofactor and nitrate reductase-type cofactor by the Azotobacter vinelandii UW-45 and Neurospora crassa nit-1 reconstitution assays, respectively. Tungsten formate dehydrogenase ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90782-4

    authors： Deaton JC,Solomon EI,Durfor CN,Wetherbee PJ,Burgess BK,Jacobs DB

    更新日期：1984-06-29 00:00:00

  • alpha-Crystallin facilitates the reactivation of hydrogen peroxide-inactivated rhodanese.

    abstract：:It was previously shown that rhodanese, inactivated with hydrogen peroxide, could only be reactivated in the presence of a reductant or the substrate thiosulfate if these reagents were added soon after inactivation and if the oxidant was removed. Here, we report on the facilitated reactivation (75%) of hydrogen peroxi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3165

    authors： Del Fierro D,Zardeneta G,Mendoza JA

    更新日期：2000-08-02 00:00:00

  • Isolation of plasma membrane fragments from cultured murine melanoma cells.

    abstract：:The various manipulations involved in the isolation of membrane fragments from culture fluids of murine melanoma cells were examined to discern their effect on membrane fragment structure. Ultracentrifugation and gel chromatography were compared using the presence of marker enzymes and the sensitivity to a non-ionic d...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91749-7

    authors： Taylor DD,Chou IN,Black PH

    更新日期：1983-06-15 00:00:00

  • Leptin enhances CC-chemokine ligand expression in cultured murine macrophage.

    abstract：:Despite accumulating evidence, the role of leptin in chemokine expression is poorly understood. In this study, we evaluated the effects of leptin on CC-chemokine ligands (CCLs), CCL3, CCL4, and CCL5 gene expression in cultured murine macrophage, J774A.1 cells. Expression of all these CCLs mRNA was gradually increased ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.121

    authors： Kiguchi N,Maeda T,Kobayashi Y,Fukazawa Y,Kishioka S

    更新日期：2009-07-03 00:00:00

  • A cancer/testis antigen, NY-SAR-35, induces EpCAM, CD44, and CD133, and activates ERK in HEK293 cells.

    abstract：:The cancer/testis (CT) antigen NY-SAR-35 gene is located on the X chromosome and is aberrantly expressed in various cancers but not in normal tissues, other than testes. Previously, we reported the expression of NY-SAR-35 enhanced cell growth, proliferation, and invasion in HEK293 and cancer cells. To extend understan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.105

    authors： Song MH,Kim YR,Bae JH,Shin DH,Lee SY

    更新日期：2017-03-04 00:00:00

  • Characterization of an alveolyn-like protein associated with mouse type 2 pneumocytes using immunochemical methods.

    abstract：:Delipidated proteins from a fraction of mouse lung homogenate enriched for lamellar bodies of type 2 pneumocytes were characterized by a combination of gel filtration and enzyme immunoassay for the presence of a surfactant-associated antigen. Immunoreactivity was associated with a protein of Mr 250-270,000 as well as ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：

    authors： Kumar RK,Smith GJ,Truscott JY,Hristoforidis CP,Lykke AW

    更新日期：1986-09-30 00:00:00

  • Heart rhythm genomic fabric in hypoxia.

    abstract：:The molecular mechanisms by which chronic hypoxia, whether constant (CCH) or intermittent (CIH), alters the heart rhythm are still under debate. Expression level, control, maturational profile and intercoordination of 54 genes encoding heart rhythm determinants (HRDs) were analyzed in 36 mice subjected for 1, 2 or 4 w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.12.151

    authors： Iacobas DA,Iacobas S,Haddad GG

    更新日期：2010-01-22 00:00:00

  • D-galactosyl-beta1-1'-sphingosine and D-glucosyl-beta1-1'-sphingosine induce human natural killer cell apoptosis.

    abstract：:Natural killer (NK) cells perform multiple biological functions including tumor cell lysis and eradicating virally infected cells. Here, we report for the first time that D-galactosyl-beta1-1' sphingosine and D-glucosyl-beta1- 1' sphingosine damage human NK cells. We show that these cells express T-cell-associated gen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.027

    authors： Maghazachi AA,Knudsen E,Jin Y,Jenstad M,Chaudhry FA

    更新日期：2004-07-30 00:00:00

  • Morphine-mimetic anti-paratypic antibodies: cross-reactive properties.

    abstract：:Polyclonal, anti-idiotypic, rabbit antibodies have been raised against four murine monoclonal anti-morphine Fab fragments. The antibody preparations, after affinity purification, have been shown to contain an anti-paratypic fraction which reversibly inhibits morphine binding to the anti-morphine antibodies and to cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90458-4

    authors： Glasel JA,Pelosi LA

    更新日期：1986-05-14 00:00:00

  • Polymorphisms of the D4 dopamine receptor alleles in chronic alcoholism.

    abstract：:We have screened genomic DNA for the identification of D4 dopamine receptor polymorphisms. We show that the D4 dopamine receptor genotype in 72 severely affected chronic alcoholics is heterogeneous, with individuals homozygous and heterozygous for the various D4 receptor alleles. Alcoholics demonstrated a greater prev...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2222

    authors： George SR,Cheng R,Nguyen T,Israel Y,O'Dowd BF

    更新日期：1993-10-15 00:00:00

  • Expression and RNA-binding of human zinc-finger antiviral protein.

    abstract：:Zinc-finger antiviral protein (ZAP) is a recently isolated host antiviral factor that inhibits the replication of many viruses such as Moloney murine leukemia virus (MLV) and Sindbis virus (SIN) by preventing the accumulation of viral mRNA in the cytoplasm. ZAP comprises four CCCH zinc-finger motifs, the second and fo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.164

    authors： Jeong MS,Kim EJ,Jang SB

    更新日期：2010-06-04 00:00:00

  • Vascular endothelial growth factor-like and its receptor in a crustacean optic ganglia: a role in neuronal differentiation?

    abstract：:The neural system appears before the vascular system in the phylogenetic tree. During evolution, vascular system generation takes advantage of the pre-existing vascular endothelial growth factor (VEGF) in order to form its networks. Nevertheless, the role of VEGF in neuronal and glial cells is not yet completely under...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.03.137

    authors： Fusco MA,Wajsenzon IJ,de Carvalho SL,da Silva RT,Einicker-Lamas M,Cavalcante LA,Allodi S

    更新日期：2014-05-02 00:00:00

  • Cathepsin Q, a novel lysosomal cysteine protease highly expressed in placenta.

    abstract：:The complete nucleotide sequence of a novel cathepsin cDNA derived from rat placenta was determined and is termed cathepsin Q. The predicted protein of 343 amino acid is a member of the family C1A protease related to cathepsin L. Rat cathepsin Q and its mouse counterpart were found highly expressed in placenta, wherea...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.2051

    authors： Sol-Church K,Frenck J,Mason RW

    更新日期：2000-01-27 00:00:00

  • Human fetal-to-adult globin gene switching in transgenic mice: persistent expression of the G gamma-globin gene in the Japanese HPFH.

    abstract：:We linked a 3.3 kb fragment containing the human gamma-globin gene together with a 5.5 kb fragment containing the human beta-globin gene. This construct was introduced into the germ line of mice. Analysis of the resulting transgenic mice showed that expression of the human gamma- and beta-globin genes were regulated a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1811

    authors： Katsube T,Tojo H,Sakaguchi T,Fukumaki Y

    更新日期：1993-07-15 00:00:00

  • VEGFR-2-specific ligand VEGF-E induces non-edematous hyper-vascularization in mice.

    abstract：:VEGF family members play important roles in angiogenesis and vascular permeability. VEGF-A-transgenic mice showed an increased vascularization with edema due to hyper-vascular permeability and subcutaneous hemorrhage as side effects. VEGF-A binds and activates two receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). To...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)03033-4

    authors： Kiba A,Sagara H,Hara T,Shibuya M

    更新日期：2003-02-07 00:00:00

  • Inhibition of the catalytic subunit of ram sperm adenylate cyclase by adenosine.

    abstract：:Adenosine inhibits ram sperm adenylate cyclase activity which is membrane-bound and comprises only the catalytic subunit. The inhibition parameters of adenylate cyclase by adenosine were not modified when the enzyme was purified 3 to 5,000 fold. Optimal inhibition by adenosine was found to require a high concentration...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90320-7

    authors： Henry D,Ferino F,Tomova S,Ferry N,Stengel D,Hanoune J

    更新日期：1986-06-30 00:00:00

  • The action of erythropoietin is mediated by lipoxygenase metabolites in murine fetal liver cells.

    abstract：:Erythroid progenitor cells synthesize 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-hydroxyeicosatetraenoic acid (15-HETE) when stimulated by erythropoietin (Ep). Maximal stimulation of 12-HETE production occurred at one hour, whereas 15-HETE activity remained constant in response to Ep for 24 hours. Lipoxygenase-s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80134-1

    authors： Beckman BS,Mason-Garcia M,Nystuen L,King L,Fisher JW

    更新日期：1987-08-31 00:00:00

  • miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN.

    abstract：:MicroRNAs play important roles in the carcinogenesis of many types of cancers by inhibiting gene expression at posttranscriptional level. However, the roles of microRNAs in hepatocellular carcinoma, are still unclear. Here, we identified that miR-367 promotes hepatocellular carcinoma (HCC) cell proliferation by negati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.bbrc.2016.01.025

    authors： Meng X,Lu P,Fan Q

    更新日期：2016-01-29 00:00:00

  • A highly conserved tyrosine residue at codon 845 within the kinase domain is not required for the transforming activity of human epidermal growth factor receptor.

    abstract：:Epidermal growth factor receptor (EGF-R) is a widely expressed ligand-dependent tyrosine kinase. The tyrosine residue at 845 in EGF-R corresponds to Y416 of v/c-src kinase, which is highly conserved and functionally important in many tyrosine kinases. To clarify the functional role of Y845, we constructed a mutant hum...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90812-y

    authors： Gotoh N,Tojo A,Hino M,Yazaki Y,Shibuya M

    更新日期：1992-07-31 00:00:00

  • Moderate hearing loss associated with a novel KCNQ4 non-truncating mutation located near the N-terminus of the pore helix.

    abstract：:Genetic mutation is one of the causative factors for idiopathic progressive hearing loss. A patient with late-onset, moderate, and high-frequency hearing loss was found to have a novel, heterozygous KCNQ4 mutation, c.806_808delCCT, which led to a p.Ser260del located between S5 and the pore helix (PH). Molecular modeli...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.01.118

    authors： Watabe T,Matsunaga T,Namba K,Mutai H,Inoue Y,Ogawa K

    更新日期：2013-03-15 00:00:00

  • Conformational dynamics of loops L11 and L12 of kinesin as revealed by spin-labeling EPR.

    abstract：:The EPR spectra of the spin labels attached to loops L11 and L12 of kinesin were resolved into slow (rotational correlation time, tau=10-45 ns) and fast (tau=2 ns) components. The fraction of the slow component increased considerably when kinesin was complexed with a microtubule (MT). On MT binding and in the presence...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.043

    authors： Yamada MD,Maruta S,Yasuda S,Kondo K,Maeda H,Arata T

    更新日期：2007-12-21 00:00:00

  • Biochemical deficiencies of coenzyme Q10 in HIV-infection and exploratory treatment.

    abstract：:AIDS patients (2 groups) had a blood deficiency (p less than 0.001) of coenzyme Q10 vs. 2 control groups. AIDS patients had a greater deficiency (p less than 0.01) than ARC patients. ARC patients had a deficiency (p less than 0.05) vs. control. HIV-infected patients had a deficiency (p less than 0.05) vs. control. The...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)81179-3

    authors： Folkers K,Langsjoen P,Nara Y,Muratsu K,Komorowski J,Richardson PC,Smith TH

    更新日期：1988-06-16 00:00:00

  • A predominant form of C-terminally end-cleaved AQP0 functions as an open water channel and an adhesion protein in AQP0ΔC/ΔC mouse lens.

    abstract：:The purpose of this investigation was to find out whether C-terminally end-cleaved aquaporin 0 (AQP0), that is present predominantly in the lens mature fiber cells of the WT, functions as a water channel and a cell-to-cell adhesion (CTCA) protein in a knockin (KI) mouse model (AQP0ΔC/ΔC) that does not express intact A...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.02.098

    authors： Kumari SS,Varadaraj K

    更新日期：2019-04-09 00:00:00

  • Eupatilin, an activator of PPARα, inhibits the development of oxazolone-induced atopic dermatitis symptoms in Balb/c mice.

    abstract：:Eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone) is the main lipophilic flavonoid obtained from the Artemisia species. Eupatilin has been reported to have anti-apoptotic, anti-oxidative and anti-inflammatory activities. Previously, we found that eupatilin increases transcriptional activity and expression of peroxis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.01.098

    authors： Jung Y,Kim JC,Park NJ,Bong SK,Lee S,Jegal H,Jin LT,Kim SM,Kim YK,Kim SN

    更新日期：2018-02-05 00:00:00

  • A new comprehensive method for detection of livestock-related pathogenic viruses using a target enrichment system.

    abstract：:We tested usefulness of a target enrichment system SureSelect, a comprehensive viral nucleic acid detection method, for rapid identification of viral pathogens in feces samples of cattle, pigs and goats. This system enriches nucleic acids of target viruses in clinical/field samples by using a library of biotinylated R...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.12.017

    authors： Oba M,Tsuchiaka S,Omatsu T,Katayama Y,Otomaru K,Hirata T,Aoki H,Murata Y,Makino S,Nagai M,Mizutani T

    更新日期：2018-01-08 00:00:00

  • Structural basis of functional mimicry between carbohydrate and peptide ligands of con A.

    abstract：:Crystallographic studies have shown independent binding sites for sugar and peptide ligands of concanavalin A, although they were considered functional mimics based on biochemical experiments. The topological correlation of 12-residue peptide with different carbohydrate ligands of concanavalin A showed similarity betw...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2871

    authors： Jain D,Kaur KJ,Goel M,Salunke DM

    更新日期：2000-06-16 00:00:00

  • Catabolite inactivation of fructose 1,6-bisphosphatase and cytoplasmic malate dehydrogenase in yeast.

    abstract：:Catabolite inactivation of fructose 1,6-bisphosphatase and cytoplasmic malate dehydrogenase was studied using the protease-deficient and vacuole-defective yeast strain pep4-3. The catabolite inactivation of fructose 1,6-bisphosphatase in pep4-3 was found to have a normal first inactivation step but with a defective se...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90440-1

    authors： Funaguma T,Toyoda Y,Sy J

    更新日期：1985-07-16 00:00:00