Abstract:
:RhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5'-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interleukin-13 (IL-13)-stimulated cells revealed a significant promoter activity at 238- to 166-bp upstream of the transcription-initiation site, which contains a signal transducer and activation of transcription (STAT) 6-binding region. The IL-13-induced increase in luciferase activity was inhibited by a STAT6 inhibitor, AS1517499, or a Janus kinases (JAKs) inhibitor, JAK Inhibitor-I, but not by tyrphostin-AG490, WHI-P131, or tyrphostin-AG9 (selective JAK2, JAK3, and Tyk2 inhibitors, respectively). Thus, rat BSM rhoA expression may have causal relation to the IL-13-JAK1-STAT6 signaling.
journal_name
J Pharmacol Scijournal_title
Journal of pharmacological sciencesauthors
Chiba Y,Goto K,Matsusue K,Kimura S,Misawa Mdoi
10.1254/jphs.09346scsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
467-72issue
4eissn
1347-8613issn
1347-8648pii
JST.JSTAGE/jphs/09346SCjournal_volume
112pub_type
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