Abstract:
:By using immunofluorostaining and confocal laser microscopy, acetylcholine-induced translocation of RhoA was visualized in freshly isolated bronchial smooth muscle cells of the rat. The cellular distribution of RhoA at rest was observed uniformly in the cytosolic space with no staining in the nucleus, whereas acetylcholine stimulation induced a relocalization of RhoA to the cell periphery. From the results of line scans and surface plots, the peripheral to cytosolic ratio of RhoA was significantly increased by acetylcholine stimulation. Thus, the present study clearly demonstrated an acetylcholine-induced translocation of RhoA to the plasma membrane in single bronchial smooth muscle cells of the rat.
journal_name
J Pharmacol Scijournal_title
Journal of pharmacological sciencesauthors
Chiba Y,Uchida T,Sakai H,Oku T,Itoh S,Tsuji T,Misawa Mdoi
10.1254/jphs.sc0040031subject
Has Abstractpub_date
2004-08-01 00:00:00pages
479-82issue
4eissn
1347-8613issn
1347-8648pii
JST.JSTAGE/jphs/SC0040031journal_volume
95pub_type
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