Abstract:
:Mitochondrial proton leak can account for almost 20% of oxygen consumption and it is generally accepted that this process contributes to basal metabolism. In order to clarify the role of basal proton leak in testicular mitochondria, we performed a comparative study with kidney and liver mitochondrial fractions. Proton leak stimulated by linoleic acid and inhibited by guanosine diphosphate (GDP) was detected, in a manner that was correlated with protein levels for uncoupling protein 2 (UCP2) in the three fractions. Modulation of proton leak had an effect on reactive oxygen species production as well as on lipid peroxidation, and this effect was also tissue-dependent. However, a possible role for the adenine nucleotide transporter (ANT) in testicular mitochondria proton leak could not be excluded. The modulation of proton leak appears as a possible and attractive target to control oxidative stress with implications for male gametogenesis.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Rodrigues AS,Lacerda B,Moreno AJ,Ramalho-Santos Jdoi
10.1002/cbf.1644subject
Has Abstractpub_date
2010-04-01 00:00:00pages
224-31issue
3eissn
0263-6484issn
1099-0844journal_volume
28pub_type
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