Proliferation- and migration-enhancing effects of ginseng and ginsenoside Rg1 through IGF-I- and FGF-2-signaling pathways on RSC96 Schwann cells.

Abstract:

:The aim of the present study is to evaluate the proliferation- and migration-enhancing effects of ginseng and its component, ginsenoside (Rg1) on RSC96 Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and mitogen-activated protein kinase (MAPK) pathways, (2) migrating and anti-scar signaling, FGF-2-uPA-MMPs.We treated RSC96 cells with different concentrations (100, 200, 300, 400, 500 microg ml(-1)) of ginseng and its constituent, Rg1 (5, 10, 15, 20, 25 microg ml(-1)). We observed a proliferative effect in a dose-dependent manner by PCNA western blotting assay, MTT assay, and wound healing test. Furthermore, we also found in the results of western blotting assay, ginseng and Rg1 enhance protein expression of IGF-I pathway regulators, cell cycle controlling proteins, and MAPK signaling pathways to promote the cell proliferation. In addition, ginseng and Rg1 also stimulated the FGF-2-uPA-MMP 9 migrating pathway to enhance the migration of RSC96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of ginseng and Rg1 on RSC96 cells were identified to be MAPK signaling-dependent. On the basis of the results, applying appropriate doses of ginseng and Rg1 with biomedical materials would be a potential approach for enhancing neuron regeneration.

journal_name

Cell Biochem Funct

authors

Lu MC,Lai TY,Hwang JM,Chen HT,Chang SH,Tsai FJ,Wang HL,Lin CC,Kuo WW,Huang CY

doi

10.1002/cbf.1554

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

186-92

issue

4

eissn

0263-6484

issn

1099-0844

journal_volume

27

pub_type

杂志文章
  • Triiodothyronine receptor sites in serum-free cultured hepatocytes from adult rat liver.

    abstract::Nuclear T3 specific binding sites were characterized by Scatchard analyses of L-125I-T3 binding to nuclei extracted from freshly isolated and 1, 2 and 6 day-cultured hepatocytes. The results demonstrate a marked decrease in T3 binding capacity of nuclei extracted from 1 day-cultured cells followed by an almost complet...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.290030203

    authors: Goglia F,Gallo G,Palmero S,Voci A,Fugassa E

    更新日期:1985-04-01 00:00:00

  • Mitochondria as the target for mildronate's protective effects in azidothymidine (AZT)-induced toxicity of isolated rat liver mitochondria.

    abstract::Previously mildronate, an aza-butyrobetaine derivative, was shown to be a cytoprotective drug, through its mechanism of action of inhibition of carnitine palmitoyltransferase-1, thus protecting mitochondria from long-chain fatty acid accumulation and subsequent damage. Recently in an azidothymidine (AZT)-induced cardi...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1486

    authors: Pupure J,Fernandes MA,Santos MS,Moreno AJ,Kalvinsh I,Klusa V,Oliveira CR

    更新日期:2008-09-01 00:00:00

  • In non-small cell lung cancer mitogenic signaling leaves Sprouty1 protein levels unaffected.

    abstract::Sprouty1 protein belongs to a family of receptor tyrosine kinase-mediated signaling inhibitors, whose members are usually regulated by growth factors to form a negative feedback loop. Correspondingly fluctuations of Sprouty1 mRNA in response to single growth factors have been observed. In this report, we investigate S...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.2976

    authors: Kral RM,Mayer CE,Vanas V,Gsur A,Sutterlüty-Fall H

    更新日期:2014-01-01 00:00:00

  • Localization of biotransformational enzymes along the crypt-villus axis of the rat intestine. Evaluation of two cell isolation procedures.

    abstract::Rat intestinal epithelial cells were isolated by EDTA-chelation, combined with gentle shaking (modified Weiser procedure) or with strong longitudinal vibration (Harrison/Webster procedure). Both methods yield large numbers of viable cells and are relatively easy to use. Electronmicroscopical and biochemical data indic...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.290020208

    authors: Koster AS,Borm PJ,Dohmen MR,Noordhoek J

    更新日期:1984-04-01 00:00:00

  • Effects of L-carnitine on neutrophil-mediated ischemia-reperfusion injury in rat stomach.

    abstract::Reactive oxygen metabolites play an important role in ischemia-reperfusion related gastric injury. Primary sources of reactive oxygen metabolites seem to be the xanthine/xanthine oxidase system and neutrophils accumulating within the reperfused tissue. Tissue myeloperoxidase activity is an important index of neutrophi...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1251

    authors: Derin N,Agac A,Bayram Z,Asar M,Izgut-Uysal VN

    更新日期:2006-09-01 00:00:00

  • The neutrophil elastase mutant affects viability and differentiation of the human monocytic THP-1 cell.

    abstract::Deficiency in neutrophils (neutropenia) caused by mutations in neutrophil elastase (NE, ELA2) has been extensively investigated. Monocytes and neutrophils are derived from a common myeloid progenitor; therefore, ELA2 mutations can also influence monocyte development. These effects have not been well described. In this...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.2900

    authors: Lee HH,Sung MH

    更新日期:2013-06-01 00:00:00

  • Antimicrobial and biological effects of ipemphos and amphos on bacterial and yeast strains.

    abstract::In this study, the antimicrobial effects of monophosphazenes such as SM ipemphos and amphos were examined on bacterial and yeast strains. In addition, the biological effects of these compounds were tested on the lipid level of Saccharomyces cerevisiae and Candida albicans cells. The SM has an antimicrobial effect on t...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/(SICI)1099-0844(200006)18:2<117::AID-CBF86

    authors: Oztürk AI,Yilmaz O,Kirbağ S,Arslan M

    更新日期:2000-06-01 00:00:00

  • Effects of thymoquinone on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase in different tissues of mice: a possible mechanism of action.

    abstract::The present investigation focused, firstly, on the effects of oral administration of thymoquinone (TQ) on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase activity in hepatic, cardiac and kidney tissues of normal mice. Superoxide dismutase (SOD; E.C:1.15.1.1), catalase (CAT; E.C:1.11.1.6), glutathio...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.968

    authors: Mansour MA,Nagi MN,El-Khatib AS,Al-Bekairi AM

    更新日期:2002-06-01 00:00:00

  • The CTIP-mediated repair of TNF-α-induced DNA double-strand break was impaired by miR-130b in cervical cancer cell.

    abstract::Chemotherapeutic drugs that induce DNA damage have the potential to kill cancer cells, but DNA repair protects cells from damage-induced cell death. Thus, eliminating DNA repair is a potential approach to overcome cell drug resistance. In this study, we observed that the gene expression of C-terminal binding protein i...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.3430

    authors: Yang L,Yang B,Wang Y,Liu T,He Z,Zhao H,Xie L,Mu H

    更新日期:2019-10-01 00:00:00

  • Human adipose-derived stromal/stem cells are distinct from dermal fibroblasts as evaluated by biological characterization and RNA sequencing.

    abstract::Human adipose-derived stromal/stem cells (ASC) have immunomodulatory properties and the potential to differentiate into several cell lines, important for application in regenerative medicine. However, the contamination with dermal fibroblasts (FIB) can impair the beneficial effects of ASC in cell therapy. It is then e...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.3610

    authors: Abreu de Melo MI,da Silva Cunha P,Coutinho de Miranda M,Faraco CCF,Barbosa JL,da Fonseca Ferreira A,Kunrath Lima M,Faria JAQA,Rodrigues MÂ,de Goes AM,Gomes DA

    更新日期:2021-01-03 00:00:00

  • The effects of garlic-derived sulfur compounds on cell proliferation, caspase 3 activity, thiol levels and anaerobic sulfur metabolism in human hepatoblastoma HepG2 cells.

    abstract::The aim of the present studies was to determine whether the mechanism of biological action of garlic-derived sulfur compounds in human hepatoma (HepG2) cells can be dependent on the presence of labile sulfane sulfur in their molecules. We investigated the effect of allyl sulfides from garlic: monosulfide, disulfide an...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1835

    authors: Iciek M,Kwiecień I,Chwatko G,Sokołowska-Jeżewicz M,Kowalczyk-Pachel D,Rokita H

    更新日期:2012-04-01 00:00:00

  • Role of protein kinases in mediating diabetes-induced augmented vasoconstriction to endothelin-1 in the renal arteries of STZ-diabetic rats.

    abstract::Diabetes is associated with increased reactivity of the renal vascular bed to endothelin-1 (ET-1). It has been observed that diabetes is associated with over-expression of ET(A)- and ET(B)-receptors in the rat renal cortex. However it is not known if these receptors are over-expressed in the renal artery. The objectiv...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1253

    authors: Yousif MH

    更新日期:2006-09-01 00:00:00

  • LncRNA TDRG1/miR-214-5p axis affects preeclampsia by modulating trophoblast cells.

    abstract::Because of limited treatment options, preeclampsia (PE) is the leading cause of perinatal morbidity and mortality worldwide. Recently, lncRNA TDRG1 is reported to be aberrantly down-regulated in PE placenta, and the abnormal expression of TDRG1 might play a key or partial role in PE development. In this study, we foun...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.3480

    authors: Gong F,Cheng H,Shi Y,Cui L,Jia G

    更新日期:2020-06-01 00:00:00

  • Quercetin modulates keratoconus metabolism in vitro.

    abstract::Corneal scarring is the result of a disease, infection or injury. The resulting scars cause significant loss of vision or even blindness. To-date, the most successful treatment is corneal transplantation, but it does not come without side effects. One of the corneal dystrophies that are correlated with corneal scarrin...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.3122

    authors: McKay TB,Sarker-Nag A,Lyon D,Asara JM,Karamichos D

    更新日期:2015-07-01 00:00:00

  • The protection by heme oxygenase-1 induction against thioacetamide-induced liver toxicity is associated with changes in arginine and asymmetric dimethylarginine.

    abstract::This study was designed to investigate the role of HO-1 induction in prevention of thioacetamide (TAA)-induced oxidative stress, inflammation and liver damage. The changes in hepatic dimethylarginine dimethylaminohydrolase (DDAH) activity as well as plasma arginine and asymmetric dimethylarginine (ADMA) levels were al...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.2866

    authors: Develi-Is S,Bekpinar S,Kalaz EB,Evran B,Unlucerci Y,Gulluoglu M,Uysal M

    更新日期:2013-03-01 00:00:00

  • Effect of bafilomycin and NAADP on membrane-associated ATPases and respiration of isolated mitochondria of the murine Nemeth-Kellner lymphoma.

    abstract::The goal of the study was to estimate the effect of a selective V-type H+ -ATPase inhibitor bafilomycin A1 and nicotinic acid adenine dinucleotide phosphate (NAADP) on energetic processes in NK/Ly cell by directly measuring the respiration of isolated mitochondria and ATPase activities. NAADP (7 μM) increased the acti...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.3231

    authors: Hreniukh V,Bychkova S,Kulachkovsky O,Babsky A

    更新日期:2016-12-01 00:00:00

  • A review of myocardial ischaemia/reperfusion injury: Pathophysiology, experimental models, biomarkers, genetics and pharmacological treatment.

    abstract::Cardiovascular diseases are known to be the most fatal diseases worldwide. Ischaemia/reperfusion (I/R) injury is at the centre of the pathology of the most common cardiovascular diseases. According to the World Health Organization estimates, ischaemic heart disease is the leading global cause of death, causing more th...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章,评审

    doi:10.1002/cbf.3587

    authors: Gunata M,Parlakpinar H

    更新日期:2020-09-06 00:00:00

  • Trikatu, a herbal compound that suppresses monosodium urate crystal-induced inflammation in rats, an experimental model for acute gouty arthritis.

    abstract::Gout is an inflammatory joint disorder characterized by hyperuricaemia and precipitation of monosodium urate crystals in the joints. In the present study, we aimed to investigate the anti-inflammatory effect of trikatu, a herbal compound in monosodium urate crystal-induced inflammation in rats, an experimental model f...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.2979

    authors: Murunikkara V,Rasool M

    更新日期:2014-01-01 00:00:00

  • Phospholipids in chromatin: incorporation of [32P]O4(2-) in different subcellular fractions of hepatocytes.

    abstract::Nuclear, chromatin and microsomal fractions were isolated from hepatocytes prepared from rats injected with [32P]O4(2-) and killed subsequently at times between 1 and 48 h. Specific activities of the total phospholipids (PL) were determined for each subcellular fraction. The major points noted were the initial specifi...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.290040409

    authors: Viola-Magni MP,Gahan PB,Albi E,Iapoce R,Gentilucci PF

    更新日期:1986-10-01 00:00:00

  • Antioxidant enzymatic defense in salivary glands of streptozotocin-induced diabetic rats: a temporal study.

    abstract::Hyperglycemia induces overproduction of superoxide and it is related to diabetic complications. In this study, we analyzed the antioxidant enzymatic defense and the lipid peroxidation of rat salivary glands in six different periods of diabetic condition. Ninety-six rats were divided into 12 groups: C7/14/21/28/45/60 (...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1683

    authors: Ibuki FK,Simões A,Nogueira FN

    更新日期:2010-08-01 00:00:00

  • Analysis of transforming growth factor beta 1 (TGF-beta1) gene polymorphisms in Turkish patients with scleroderma.

    abstract::Systemic sclerosis (SSc) is an autoimmune disease characterized by inflammation and fibrosis of the skin and visceral organs. Fibrosis associated with SSc is characterized by an increased synthesis of a wide range of extracellular matrix (ECM). TGF-beta is a pluripotent cytokine in a wide range of cell types. In parti...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1649

    authors: Büyük U,Ates O,Dalyan L,Müsellim B,Ongen G,Topal-Sarikaya A

    更新日期:2010-06-01 00:00:00

  • NBD-cholesterol incorporation by rat macrophages and lymphocytes: a process dependent on the activation state of the cells.

    abstract::The time-course of incorporation of NBD-cholesterol by macrophages (Ma) and lymphocytes (LY) obtained from untreated and thioglycollate-injected (thio) rats was investigated. NBD-cholesterol incorporation was also examined in Ma obtained from untreated rats and stimulated in vitro by lipopolysaccharide (LPS) and phorb...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1048

    authors: Portioli Silva EP,Peres CM,Roberto Mendonça J,Curi R

    更新日期:2004-01-01 00:00:00

  • JAR human placental choriocarcinoma cells actively synthesize, take up and release polyamines.

    abstract::Uptake of polyamines has been investigated extensively in many cells, but not in placenta, where the polyamine-polyamine oxidase system is supposed to have an immunoregulatory function in pregnancy. Due to the importance of the transfer in this tissue, we have started this study. JAR human placental choriocarcinoma ce...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.664

    authors: Fontana L,Cravanzola C,Colombatto S,Grillo MA

    更新日期:1996-09-01 00:00:00

  • Prostate cancer is known by the companionship with ATM and miRNA it keeps: craftsmen of translation have dual behaviour with tailors of life thread.

    abstract::Research on prostate cancer progression has focused extensively on the concept of miRNA, which can operate either as promoters or as suppressors of carcinogenesis. Moreover, recent genetic studies and emerging functional work show that strikingly similar and overlapping pathways are involved in prostate carcinogenesis...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章,评审

    doi:10.1002/cbf.2847

    authors: Farooqi AA,Naqi A,Qureshi MZ,Rana A,Khan A,Riaz AM,Afzal SM,Rasheed N,Bhatti S

    更新日期:2012-10-01 00:00:00

  • Chemoprotective effects of carnosine against genotoxicity induced by cyclophosphamide in mice bone marrow cells.

    abstract::The protective effects of carnosine as a natural dipeptide were investigated in mouse bone marrow cells against genotoxicity induced by cyclophosphamide. Mice were injected with solutions of carnosine at three different doses (10, 50 and 100 mg kg(-1) bw) for five consecutive days. On the fifth day of treatment, mice ...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.2834

    authors: Naghshvar F,Abianeh SM,Ahmadashrafi S,Hosseinimehr SJ

    更新日期:2012-10-01 00:00:00

  • Genetic instability of RBM5/LUCA-15/H37 in MCF-7 breast carcinoma sublines may affect susceptibility to apoptosis.

    abstract::The MCF-7 human breast carcinoma cell line is widely used as a model system by breast cancer researchers and cell biologists investigating apoptosis. Since its establishment 30 years ago, from a patient with metastatic breast cancer, the original MCF-7 cell population has undergone genetic drift to such an extent that...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.1106

    authors: Rintala-Maki ND,Abrasonis V,Burd M,Sutherland LC

    更新日期:2004-09-01 00:00:00

  • Fibronectin is not a serum spreading factor for HeLa cells.

    abstract::The spreading of HeLa cells on plastic substratum is mediated by fibronectin-depleted foetal calf serum but not by fibronectin isolated by gelatin-Sepharose affinity chromatography. The same is true for freshly explanted chick embryonic chondrocytes. In contrast, BHK cell spreading exceeds 67% after 120 min at 37 degr...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.290010208

    authors: Michl J,Baudysová M,Sochorová L,Deyl Z

    更新日期:1983-07-01 00:00:00

  • Circ_LAS1L regulates cardiac fibroblast activation, growth, and migration through miR-125b/SFRP5 pathway.

    abstract::Current studies have shown that circular RNAs (circRNAs) and microRNAs (miRNAs) are closely related in acute myocardial infarction (AMI). Previous studies have shown that miR-125b promotes fibrosis and up-regulates in cardiac fibroblasts (CFs), and further experiments showed that circ_LAS1L had multiple binding sites ...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.3486

    authors: Sun LY,Zhao JC,Ge XM,Zhang H,Wang CM,Bie ZD

    更新日期:2020-06-01 00:00:00

  • Leucine improves protein nutritional status and regulates hepatic lipid metabolism in calorie-restricted rats.

    abstract::Several studies have highlighted the potential of leucine supplementation for the treatment of metabolic diseases including type 2 diabetes and obesity. Caloric restriction is a common approach to improve the health in diabetic and obese subjects. However, very few studies assessed the effects of leucine supplementati...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.3017

    authors: Pedroso JA,Nishimura LS,de Matos-Neto EM,Donato J Jr,Tirapegui J

    更新日期:2014-06-01 00:00:00

  • Methotrexate: studies on cellular metabolism. II--Effects on mitochondrial oxidative metabolism and ion transport.

    abstract::Effects of methotrexate (MTX) on mitochondrial oxidative metabolism and ion transport were studied. MTX decreases the membrane potential (delta psi) upon energization of the mitochondrial membrane by NAD+-linked substrates and decreases the amplitude and velocity of swelling induced by glutamate and alpha-ketoglutarat...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章

    doi:10.1002/cbf.290070208

    authors: Yamamoto N,Lopes LC,Campello AP,Klüppel ML

    更新日期:1989-04-01 00:00:00