Abstract:
:The time-course of incorporation of NBD-cholesterol by macrophages (Ma) and lymphocytes (LY) obtained from untreated and thioglycollate-injected (thio) rats was investigated. NBD-cholesterol incorporation was also examined in Ma obtained from untreated rats and stimulated in vitro by lipopolysaccharide (LPS) and phorbol-myristate acetate (PMA). The same measurement was performed in LY from untreated rats stimulated by addition of LPS and concanavalin A (Con A) into the culture medium. Thio-treated Ma showed high fluorescence intensity after 1 h of incubation with NBD-cholesterol. Ma submitted concomitant to LPS and NBD-cholesterol showed low fluorescence intensity, as well as Ma stimulated with PMA. Ma from untreated and LPS pre-treated rats showed a similar time-course of incorporation. LY from thio-treated rats showed lower incorporation of NBD-cholesterol in comparison to LY from untreated rats. Incorporation was reduced when LPS was added concomitantly with NBD-cholesterol. On the other hand, LY pre-treated with LPS for 48 h showed a very high incorporation of NBD-cholesterol. Con A treatment did not cause a significant effect on NBD-cholesterol incorporation. The findings presented herein led us to conclude that the uptake of NBD-cholesterol by Ma and LY is markedly affected by the activation state of the cells.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Portioli Silva EP,Peres CM,Roberto Mendonça J,Curi Rdoi
10.1002/cbf.1048subject
Has Abstractpub_date
2004-01-01 00:00:00pages
23-8issue
1eissn
0263-6484issn
1099-0844journal_volume
22pub_type
杂志文章abstract::Although techniques for purifying epidermal stem cells (ESCs) have been established, enriching a pure population of viable ESCs is still a challenging task. One approach toward enhancing the purity and viability of ESCs involves cell markers. While evidence suggests that p63 plays a role in maintaining the population ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1612
更新日期:2009-12-01 00:00:00
abstract::Psoriasis is a common chronic and recurrent inflammatory skin disease with unknown etiology that has been associated with abnormal plasma lipid metabolism and oxidative stress. There are controversial results in the previous studies investigating oxidant/antioxidant systems in psoriasis.The aim of this work was to eva...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1553
更新日期:2009-04-01 00:00:00
abstract::A previous study showing that ethanol (ETOH) blocked [3H]choline incorporation into phosphatidylcholine (PC) suggested an inhibition of PC biosynthesis in human leukemic monocyte-like U937 cells. The mechanism of the inhibitory action of ETOH was investigated. Cells were pulsed with [3H]choline for 30 min and chased i...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120107
更新日期:1994-01-01 00:00:00
abstract::Mast cells are widely distributed in the body and affect their surrounding environment through degranulation and secretion of cytokines. Conversely, mast cells are influenced by environmental stimuli such as cyclical mechanical stretch (CMS), such as that induced by heartbeat and respiration. Peripherally distributed ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2973
更新日期:2014-01-01 00:00:00
abstract::The aim of this experimental study was to investigate the possible role of adenosine deaminase (AD) and xanthine oxidase (XO) in the pathogenesis of cisplatin-induced nephrotoxicity and the effect of erdosteine in decreasing the toxicity. The intraperitoneal injection of cisplatin (7 mg kg(-1) body weight) induced a s...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1069
更新日期:2004-05-01 00:00:00
abstract::Human cytochrome P450 2J2 (CYP2J2) is abundant in cardiovascular tissue and active in the metabolism of arachidonic acid to eicosanoids that have potent vasodilatory properties. Variability of the CYP2J2 gene is highly constrained except for its proximal promoter: there is a relatively common and functionally relevant...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1512
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of artemisinin on the proliferation and apoptosis of rat vascular smooth muscle cells (VSMCs). METHOD:Primary rat VSMCs were treated with various doses of artemisinin. Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2995
更新日期:2014-03-01 00:00:00
abstract::In this study, the antimicrobial effects of monophosphazenes such as SM ipemphos and amphos were examined on bacterial and yeast strains. In addition, the biological effects of these compounds were tested on the lipid level of Saccharomyces cerevisiae and Candida albicans cells. The SM has an antimicrobial effect on t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(200006)18:2<117::AID-CBF86
更新日期:2000-06-01 00:00:00
abstract::Nuclear T3 specific binding sites were characterized by Scatchard analyses of L-125I-T3 binding to nuclei extracted from freshly isolated and 1, 2 and 6 day-cultured hepatocytes. The results demonstrate a marked decrease in T3 binding capacity of nuclei extracted from 1 day-cultured cells followed by an almost complet...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030203
更新日期:1985-04-01 00:00:00
abstract::The aim of the present study is to evaluate the proliferation- and migration-enhancing effects of ginseng and its component, ginsenoside (Rg1) on RSC96 Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle fac...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1554
更新日期:2009-06-01 00:00:00
abstract::Thyroid cells fall into the type of cells functioning during continuous production of high H(2)O(2) concentrations. We studied the effect of H(2)O(2)-induced oxidative stress (0.1, 1.0 and 10.0 mM) on the activities of the key steps of iodide metabolism (uptake, oxidation and organification) in thyrocytes cultivated i...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1452
更新日期:2008-04-01 00:00:00
abstract::During remodelling of pulmonary artery, marked proliferation of pulmonary artery smooth muscle cells (PASMCs) occurs, which contributes to pulmonary hypertension. Thromboxane A2 (TxA2) has been shown to produce pulmonary hypertension. The present study investigates the inhibitory effect of epigallocatechin-3-gallate (...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3136
更新日期:2015-10-01 00:00:00
abstract::The study aims to evaluate the effect of saxagliptin, a specific inhibitor of dipeptidyl peptidase-4 enzymes, on body weight gain, lipid profiles, and cell death through apoptosis in rats with insulin resistance (IR). Male adult Sprague-Dawley rats (n = 32) were divided into 4 groups: control (Ctrl), IR, saxagliptin c...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3333
更新日期:2018-06-01 00:00:00
abstract::The use of Nigerian bonny light crude oil (BLCO) in the treatment of gastrointestinal disorders, burns, foot ulcers and reproductive capacity is a common practice in the southern part of Nigeria. Towards understanding the mechanism and the reversibility of hepatotoxicity induced by BLCO, adult male Wistar rats were or...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2823
更新日期:2012-08-01 00:00:00
abstract::Systemic sclerosis (SSc) is an autoimmune disease characterized by inflammation and fibrosis of the skin and visceral organs. Fibrosis associated with SSc is characterized by an increased synthesis of a wide range of extracellular matrix (ECM). TGF-beta is a pluripotent cytokine in a wide range of cell types. In parti...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1649
更新日期:2010-06-01 00:00:00
abstract::Thyroid hormones regulate energy metabolism and act on mitochondria which are an important source of free radicals in the cell. The pineal gland activates antioxidant systems via melatonin secretion and thus has a protective function in body tissues. The present study was conducted to determine the oxidative damage ca...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1254
更新日期:2006-09-01 00:00:00
abstract::Sprouty1 protein belongs to a family of receptor tyrosine kinase-mediated signaling inhibitors, whose members are usually regulated by growth factors to form a negative feedback loop. Correspondingly fluctuations of Sprouty1 mRNA in response to single growth factors have been observed. In this report, we investigate S...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2976
更新日期:2014-01-01 00:00:00
abstract::The present investigation focused, firstly, on the effects of oral administration of thymoquinone (TQ) on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase activity in hepatic, cardiac and kidney tissues of normal mice. Superoxide dismutase (SOD; E.C:1.15.1.1), catalase (CAT; E.C:1.11.1.6), glutathio...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.968
更新日期:2002-06-01 00:00:00
abstract::Gastrin-releasing peptide (GRP) receptors are present in pancreatic islets, though their regulation is unknown except for homologous desensitization. The modulation of binding of GRP to mouse pancreatic islets and INS-1 cells was studied. At 60 min (steady-state), total binding of [(125)I-Tyr(15)] GRP was 1.62 per cen...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199912)17:4<229::AID-CBF83
更新日期:1999-12-01 00:00:00
abstract::Analysis of free nucleotide composition of both avian thrombocytes and pig platelets showed quantitative differences in the level of adenine nucleotides. 3H-adenine taken up by turkey thrombocytes was metabolized mainly to adenine nucleotides was not released after thrombin action. Thrombin liberated non-radioactive a...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020310
更新日期:1984-07-01 00:00:00
abstract::The effect of citrinin on Ca2+ transport was studied in isolated kidney cortex and liver mitochondria, and baby hamster kidney cultured cells. The mycotoxin significantly inhibited the activity of 2-oxoglutarate and pyruvate dehydrogenases in both kidney cortex and liver mitochondria. Citrinin promoted a decrease in t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290130110
更新日期:1995-03-01 00:00:00
abstract::Dietary soy lecithin supplementation decreases hyperlipidemia and influences lipid metabolism. Although this product is used by diabetic patients, there are no data about the effect of soy lecithin supplementation on the immune system. The addition of phosphatidylcholine, the main component of lecithin, to a culture o...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1517
更新日期:2008-12-01 00:00:00
abstract::The ataxia-telangiectasia mutated (ATM) protein kinase is best known for its critical nuclear roles in the DNA damage response (DDR), cell cycle checkpoints, and the maintenance of gene stability. In this review, we highlight the multifaceted cytoplasmic functions of ATM in autophagy. We focused on the functions of AT...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3385
更新日期:2019-04-01 00:00:00
abstract::The aim of this brief communication was to determine the correlation between pre-eclampsia (PE), Helicobacter pylori pathogenic strains seropositivity, and interleukin-18 (IL-18) levels. To this purpose 25 pre-eclamptic women and 25 healthy parturient women of similar age were evaluated for: IL-18 levels, by ELISAH. p...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1503
更新日期:2008-10-01 00:00:00
abstract::The aim of our study was to evaluate the levels of fibronectin (FN) and its classic receptor (FNR) in various transformed cells lines, especially of leukemic origin, and also the influence of HIV-1 replication on the expression of these proteins (in particular on H9-V cells, chronically infected with HIV-1, and acutel...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.665
更新日期:1996-06-01 00:00:00
abstract::The requirement of large amounts of the recombinant human bone morphogenetic protein-2 (BMP-2) produces a huge translational barrier for its routine clinical use due to high cost. This leads to an urgent need to develop alternative methods to lower costs and/or increase efficacies for using BMP-2. In this study, we de...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1615
更新日期:2009-12-01 00:00:00
abstract::Extracellular acylglycerols are hydrolysed by lipases active at the surface of intact fat cells isolated from rat or human adipose tissue. During short-term incubation, rat fat cells hydrolyse di-[3H]oleyl-[14C]glycerol at a rate of 70 +/- 7.7 mU/10(6) cells (mean +/- S.E.) versus 440 +/- 62 mU/10(6) cells for the hyd...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290050304
更新日期:1987-07-01 00:00:00
abstract::Glucocorticoids are important for skeletal muscle energy metabolism, regulating glucose utilization, insulin sensitivity, and muscle mass. Nicotinamide adenine dinucleotide phosphate-dependent 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1)-mediated glucocorticoid activation in the sarcoplasmic reticulum (SR) is in...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3272
更新日期:2017-07-01 00:00:00
abstract::Proliferating-cell nuclear antigen (PCNA), also known as cyclin, is synthesized in proliferative cells and recently was identified as DNA polymerase-delta auxiliary protein. In this paper, the association of PCNA to the proliferative cells of plants was analysed using both autoantibodies to PCNA obtained from a patien...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100209
更新日期:1992-06-01 00:00:00
abstract::The melastatin-related transient receptor potential channel TRPM2 is a plasma membrane Ca(2+)-permeable cation channel that is activated by hydrogen peroxide (H(2)O(2)) as a consequence of oxidative stress although the channel activation by H(2)O(2) appears to represent a cell-specific process in cells with endogenous...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1310
更新日期:2007-07-01 00:00:00