Abstract:
:A previous study showing that ethanol (ETOH) blocked [3H]choline incorporation into phosphatidylcholine (PC) suggested an inhibition of PC biosynthesis in human leukemic monocyte-like U937 cells. The mechanism of the inhibitory action of ETOH was investigated. Cells were pulsed with [3H]choline for 30 min and chased in the presence or absence of ETOH for up to 6 h. PC biosynthesis was inhibited drastically within 1 h after exposure to ETOH which increased intracellular cAMP appreciably. After a 3-h treatment, ETOH significantly inhibited both choline kinase (CK) and the cytosolic CTP: cholinephosphate cytidylyltransferase (CT). The inactivated CT was no longer stimulated by exogenous phosphatidylglycerol (PG). There was no evidence for redistribution of CT activity between cytosol and microsomes. When cells were exposed to 8-Bromo-cAMP ranging from 100 to 300 microM, PC biosynthesis remained unaffected despite the drastically elevated cAMP. These results seem to suggest that the raised cAMP is not a prerequisite for the inhibition of PC biosynthesis in U937 cells. Following pretreatment with protein kinase inhibitors (H-89 and K-252a), PC biosynthesis was decreased significantly and the inhibitory effect of ETOH was potentiated. Taken together, our results suggest that the inhibition of PC biosynthesis and the inhibitory effect of ETOH are independent of the activation of cAMP-dependent protein kinase. Unlike protein kinase inhibitors, pretreatment with tyrosine kinase inhibitors (erbstatin, genistein and tyrphostin 25) resulted in differential effects on PC biosynthesis and on the inhibitory action of ETOH. Genistein stimulated PC biosynthesis by 30 per cent as well as partially preventing/reversing the ETOH action, while tyrphostin 25 produced a synergistic inhibition. The relevance of tyrosine phosphorylation/dephosphorylation to the regulation of PC biosynthesis and ETOH action remains to be established.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Chu AJdoi
10.1002/cbf.290120107subject
Has Abstractpub_date
1994-01-01 00:00:00pages
45-55issue
1eissn
0263-6484issn
1099-0844journal_volume
12pub_type
杂志文章abstract::Differences in metabolism of drugs can lead to severe toxicity or therapeutic failure. In addition to cytochrome P450 2D6, which plays a critical role in drug metabolism, ABCB1 encoded P-glycoprotein (PGP) is also an important determinant in drug bioavailability. The genes encoding these molecules are highly variable ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1561
更新日期:2009-06-01 00:00:00
abstract::Liver-directed gene therapy has become a promising treatment for many liver diseases. In this study, we constructed a multi-functional targeting molecule, which maintains targeting, endosome-escaping, and DNA-binding abilities for gene delivery. Two single oligonucleotide chains of Melittin (M) were synthesized. The f...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2958
更新日期:2013-12-01 00:00:00
abstract::The aim of this work was to determine the role of intraperitoneally-administered vitamin E and selenium on the biochemical and haematological parameters in the blood of rats. Thirty-two adult male Wistar rats were used in this study. All rats were randomly divided into four groups. The first group was used as the cont...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199906)17:2<143::AID-CBF80
更新日期:1999-06-01 00:00:00
abstract::Regeneration remains a major challenge in skeletal muscle repair after injury. Recently, transforming growth factor-β (TGF-β)/Smad pathway was found to play an important role in inhibiting myogenesis, a crucial stage in skeletal muscle regeneration. In our previous study, microRNA-122-5p (miR-122) was proved to have t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3460
更新日期:2020-03-01 00:00:00
abstract::The microsomes from dehydroepiandrosterone (DHEA)-supplemented animals are good hydroxyl radical scavengers, as demonstrated through electron spin resonance and deoxyribose degradation. The ability of DHEA-supplemented microsomes to react with superoxide radical was also demonstrated through the inhibition of nitroblu...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199803)16:1<57::AID-CBF771
更新日期:1998-03-01 00:00:00
abstract::Epidermal growth factor plays a major role in breast cancer cell proliferation, survival, and metastasis. Quercetin, a bioactive flavonoid, is shown to exhibit anticarcinogenic effects against various cancers including breast cancer. Hence, the present study was designed to evaluate the effects of gold nanoparticles-c...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3266
更新日期:2017-06-01 00:00:00
abstract::In this study, the antimicrobial effects of monophosphazenes such as SM ipemphos and amphos were examined on bacterial and yeast strains. In addition, the biological effects of these compounds were tested on the lipid level of Saccharomyces cerevisiae and Candida albicans cells. The SM has an antimicrobial effect on t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(200006)18:2<117::AID-CBF86
更新日期:2000-06-01 00:00:00
abstract::Several studies have highlighted the potential of leucine supplementation for the treatment of metabolic diseases including type 2 diabetes and obesity. Caloric restriction is a common approach to improve the health in diabetic and obese subjects. However, very few studies assessed the effects of leucine supplementati...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3017
更新日期:2014-06-01 00:00:00
abstract::Incidence and mortality rates of cancer continue to increase greatly despite the improved diagnostic and therapeutic methods. Based on GLOBOCAN estimates, the numbers of new cancer cases reported in 2018 were ~18.1 million, while the numbers of cancer mortalities were ~9.6 million. It remains difficult to diagnose mos...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3601
更新日期:2020-11-06 00:00:00
abstract::Hyperglycemia induces overproduction of superoxide and it is related to diabetic complications. In this study, we analyzed the antioxidant enzymatic defense and the lipid peroxidation of rat salivary glands in six different periods of diabetic condition. Ninety-six rats were divided into 12 groups: C7/14/21/28/45/60 (...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1683
更新日期:2010-08-01 00:00:00
abstract::The phospholipid profiles of the myocardium (from 10- and 18-day old chick embryos and 13-day old chick) and their in vitro response to the endogenous lipolytic enzymes (mainly of the phospholipase group) at pH 7.4 and 38 degrees C for 60 min were analyzed by TLC technology and densitometry. Cardiolipin (CL) was shown...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1422
更新日期:2007-09-01 00:00:00
abstract::Rat liver nonparenchymal cells (NPC) were prepared by pronase digestion and purified on discontinuous gradients on Nycodenz. Morphological and biochemical characterization of cell suspensions showed that they were free of contamination by hepatocytes. We have confirmed the usefulness of pyruvate kinase activity in mon...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290070406
更新日期:1989-10-01 00:00:00
abstract::Gout is an inflammatory joint disorder characterized by hyperuricaemia and precipitation of monosodium urate crystals in the joints. In the present study, we aimed to investigate the anti-inflammatory effect of trikatu, a herbal compound in monosodium urate crystal-induced inflammation in rats, an experimental model f...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2979
更新日期:2014-01-01 00:00:00
abstract::The protective effects of betaine in ethanol hepatotoxicity were investigated in 24 female wistar albino rats. Animals were divided into three groups: control, ethanol and ethanol + betaine group. Animals were fed liquid diets and consumed approximately 60 diet per day. Rats were fed ethanol 8 kg(- 1) day(- 1). The et...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.926
更新日期:2001-12-01 00:00:00
abstract::The aim of the present study was to measure plasma uric acid (UA) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and xanthine oxidase (XO) activities and to evaluate the relationship between these parameters and the severity of pre-eclampsia. Twenty-five pre-eclamptic, 15 healthy pregnant and 1...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1090
更新日期:2004-07-01 00:00:00
abstract::Regulation of extracellular matrix (ECM) components is essential for tissue homeostasis and function. We screened a small peptide that induces ECM protein synthesis for its usefulness in protecting keratinocytes. In this report, we demonstrate that myristoyl tetrapeptide Ala-Ala-Pro-Val (mAAPV) stimulates the expressi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3070
更新日期:2014-12-01 00:00:00
abstract::The levels of polyamines and their synthesizing enzymes in squamous cell carcinoma of prostate implanted in intact as well as castrated male rats were determined after certain hormonal manipulations. The tumour was found to grow with an identical rate in non-castrated and castrated rats. Polyamine content and activiti...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030305
更新日期:1985-07-01 00:00:00
abstract::Silibinin, an effective anti-cancer and chemopreventive agent in various epithelial cancer models, has been reported to inhibit cancer cell growth through mitogenic signalling pathways including cervical cancer. However, the underlying mechanisms are still not well elucidated. Here, we assessed the effect of silibinin...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1842
更新日期:2012-04-01 00:00:00
abstract::The use of Nigerian bonny light crude oil (BLCO) in the treatment of gastrointestinal disorders, burns, foot ulcers and reproductive capacity is a common practice in the southern part of Nigeria. Towards understanding the mechanism and the reversibility of hepatotoxicity induced by BLCO, adult male Wistar rats were or...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2823
更新日期:2012-08-01 00:00:00
abstract::Although techniques for purifying epidermal stem cells (ESCs) have been established, enriching a pure population of viable ESCs is still a challenging task. One approach toward enhancing the purity and viability of ESCs involves cell markers. While evidence suggests that p63 plays a role in maintaining the population ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1612
更新日期:2009-12-01 00:00:00
abstract::Lipopolysaccharide (LPS)-induced inflammatory factors production by the cerebral cortical glial cells in two sides of the murine brain are different. To determine if microglial cells, a subset of glial cells, are involved in asymmetric production, interleukin-6 (IL-6), interleukin-1β (IL-1β) and nitric oxide (NO) resp...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1718
更新日期:2011-01-01 00:00:00
abstract::Cyclophilin A (Cyp A), a member of the peptidyl-prolyl isomerase (PPI) family, may function as a molecular signalling switch. Comparative proteomic studies have identified Cyp A as a potential downstream target of protein kinase B (Akt). This study confirmed that Cyp A is a downstream effector of the phosphatidylinosi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3156
更新日期:2015-12-01 00:00:00
abstract::We report a functional characterisation of the novel protein MGC13096. MGC13096 over-expression restrained proliferation of HEK293T cells. DNA/flow cytometry analysis showed that the over-expression of MGC13096 severely delays cell cycle progression at S phase. Transcription factors such as activator protein-1 (AP-1),...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1410
更新日期:2008-03-01 00:00:00
abstract::The flavanone hesperetin is known to decrease basal glucose uptake, although the inhibitory mechanism is largely unknown. Here, we used MDA-MB-231 breast cancer cells to investigate the molecular pathways affected by hesperetin. The results indicate that the suppression of glucose uptake is caused by the down-regulati...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2905
更新日期:2013-07-01 00:00:00
abstract::The effect of influenza strains A (H3N2) and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(19970601)15:2<87::AID-CBF7
更新日期:1997-06-01 00:00:00
abstract::The effects of several novel monosaccharides upon thymidine incorporation into both normal and tumour cells were investigated. The monosaccharide 2-deoxy-3-[1-(R)-(ethoxycarbonyl)ethyl]- alpha-D-allo-pyranose had the most inhibitory effect on proliferation, with the (S)-enantiomer having less inhibitory effects. The c...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199712)15:4<243::AID-CBF74
更新日期:1997-12-01 00:00:00
abstract::Glucocorticoids are important for skeletal muscle energy metabolism, regulating glucose utilization, insulin sensitivity, and muscle mass. Nicotinamide adenine dinucleotide phosphate-dependent 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1)-mediated glucocorticoid activation in the sarcoplasmic reticulum (SR) is in...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3272
更新日期:2017-07-01 00:00:00
abstract::Bronchopulmonary dysplasia (BPD) is a common complication of premature birth that seriously affects the survival rate and quality of life among preterm neonates. Long non-coding RNAs (lncRNAs) have been implicated in many human diseases. However, the role of lncRNAs in the pathogenesis of BPD remains poorly understood...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3190
更新日期:2016-07-01 00:00:00
abstract::Familial Mediterranean Fever (FMF) is an autosomal recessive hereditary disease leading mostly to renal failure and nephrotic syndrome. The ultrastructure of kidney has not been fully investigated in FMF associated renal disease. The aim of this study is to provide further evidence on the ultrastructure of kidney in p...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1286
更新日期:2007-07-01 00:00:00
abstract::Functional irregularities due to damage after ischaemia-reperfusion vary depending upon the organs affected. High energy phosphates such as ATP and ADP are destroyed after ischaemia-reperfusion damage. Subsequently, protons and inorganic phosphates accumulate within the cells and the proton pumps such as adenosine tri...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.912
更新日期:2001-09-01 00:00:00