Abstract:
:The phospholipid profiles of the myocardium (from 10- and 18-day old chick embryos and 13-day old chick) and their in vitro response to the endogenous lipolytic enzymes (mainly of the phospholipase group) at pH 7.4 and 38 degrees C for 60 min were analyzed by TLC technology and densitometry. Cardiolipin (CL) was shown to be one of the major phospholipids of the chick embryo myocardium and its concentration increased as the chick embryo advanced in development. Monolysocardiolipin (MLCL) was produced subsequent to in vitro incubation of whole tissue homogenates in all myocardia studied as well as a concurrent reduction in CL. This deacylation of CL increased in magnitude as the chick embryo advanced in development indicating its age relatedness. The level of phosphatidyl ethanolamine (PE) plasmalogen was also high in all myocardia studied. Lyso alkenyl PE (LPE) was produced subsequent to in vitro incubation and its level increased as the chick embryo advanced in development, indicating PLA(2) action on the sn-2 fatty acid of PE. Phosphatidyl choline (PC) plasmalogen was also present in the chick embryo myocardium and its level increased gradually as the chick embryo advanced in development. In contrast, yolk-sac membrane contains very minute amounts of CL and PE. No PC was detected and no LPE was formed following in vitro incubation. The yolk of the unfertilized chicken egg has no CL and has very minute amounts of PE, no PC and no lysophospholipids were detected following in vitro incubation in all samples analyzed.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Helmy FM,Aikins A,Hughes J,Belfield C,Juracka Adoi
10.1002/cbf.1422subject
Has Abstractpub_date
2007-09-01 00:00:00pages
571-9issue
5eissn
0263-6484issn
1099-0844journal_volume
25pub_type
杂志文章abstract::In response to concanavalin A, cytoplasmic calcium movement was observed in human platelets, both in the presence of 1 mM Ca2+ or 1 mM EGTA in the medium. Concanavalin A also caused the activation of inositide turnover and the production of inositol phosphates, suggesting that activation of phospholipase C occurs. The...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100109
更新日期:1992-03-01 00:00:00
abstract::This study evaluated the effect of possible synergic interaction between high fat diet (HF) and hydrochlorothiazide (HCTZ) on biochemical parameters of oxidative stress in brain. Rats were fed for 16 weeks with a control diet or with an HF, both supplemented with different doses of HCTZ (0.4, 1.0, and 4.0 g kg(-1) of ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1599
更新日期:2009-10-01 00:00:00
abstract::Betulin is a principal component of birch bark and is known to possess a broad range of biological activities, including antiinflammatory, antiviral and anticancer actions. The present study was carried out in vitro to clarify the influence of betulin on melanocortin (MC) receptor-ergic signalling by using COS-7 cells...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1427
更新日期:2007-09-01 00:00:00
abstract::Reactive oxygen species (ROS) have been implicated in the aetiology of several pathological and degenerative diseases. The protective effect of natural products possessing antioxidant properties has played a crucial role in ameliorating these deleterious effects. This study investigated the chemoprotective properties ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2933
更新日期:2013-10-01 00:00:00
abstract::The modification of myeloperoxidase and lactoperoxidase with 2-(O-methoxypolethylene glycol)-4, 6-dichloro-s-triazine, an activated polyethylene glycol (PEG1), was investigated. The modification caused a shift of the Soret band in the light absorption spectrum, from 430 nm to 418 nm in the case of myeloperoxidase (nat...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199806)16:2<123::AID-CBF77
更新日期:1998-06-01 00:00:00
abstract::The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Te...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020112
更新日期:1984-01-01 00:00:00
abstract::Previously mildronate, an aza-butyrobetaine derivative, was shown to be a cytoprotective drug, through its mechanism of action of inhibition of carnitine palmitoyltransferase-1, thus protecting mitochondria from long-chain fatty acid accumulation and subsequent damage. Recently in an azidothymidine (AZT)-induced cardi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1486
更新日期:2008-09-01 00:00:00
abstract::The possibility that inositol lipid metabolism is related to nuclear events accompanying steroid hormone action has been investigated by comparing lipid phosphorylation and breakdown in normal rat liver nuclei and in hypo- and hypercortisolemic conditions. Lipid phosphorylation in vitro showed the presence of diacylgl...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120308
更新日期:1994-09-01 00:00:00
abstract::In this study, the antimicrobial effects of monophosphazenes such as SM ipemphos and amphos were examined on bacterial and yeast strains. In addition, the biological effects of these compounds were tested on the lipid level of Saccharomyces cerevisiae and Candida albicans cells. The SM has an antimicrobial effect on t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(200006)18:2<117::AID-CBF86
更新日期:2000-06-01 00:00:00
abstract::The consumption of protein supplements containing amino acids is increasing around the world. Aspartate (Asp) and asparagine (Asn) are amino acids metabolized by skeletal muscle. This metabolism involves biochemical pathways that are involved in increasing Krebs cycle activity via anaplerotic reactions, resulting in h...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1606
更新日期:2009-12-01 00:00:00
abstract::Ovarian cancer is one of the most common female malignancies, and cisplatin-based chemotherapy is routinely used in locally advanced ovarian cancer patients. Acquired or de novo cisplatin resistance remains the barrier to patient survival, and the mechanisms of cisplatin resistance are still not well understood. In th...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3319
更新日期:2018-03-01 00:00:00
abstract::Cyclophilin A (Cyp A), a member of the peptidyl-prolyl isomerase (PPI) family, may function as a molecular signalling switch. Comparative proteomic studies have identified Cyp A as a potential downstream target of protein kinase B (Akt). This study confirmed that Cyp A is a downstream effector of the phosphatidylinosi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3156
更新日期:2015-12-01 00:00:00
abstract::Sprouty1 protein belongs to a family of receptor tyrosine kinase-mediated signaling inhibitors, whose members are usually regulated by growth factors to form a negative feedback loop. Correspondingly fluctuations of Sprouty1 mRNA in response to single growth factors have been observed. In this report, we investigate S...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2976
更新日期:2014-01-01 00:00:00
abstract::Silibinin, an effective anti-cancer and chemopreventive agent in various epithelial cancer models, has been reported to inhibit cancer cell growth through mitogenic signalling pathways including cervical cancer. However, the underlying mechanisms are still not well elucidated. Here, we assessed the effect of silibinin...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1842
更新日期:2012-04-01 00:00:00
abstract::Chemotherapeutic drugs that induce DNA damage have the potential to kill cancer cells, but DNA repair protects cells from damage-induced cell death. Thus, eliminating DNA repair is a potential approach to overcome cell drug resistance. In this study, we observed that the gene expression of C-terminal binding protein i...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3430
更新日期:2019-10-01 00:00:00
abstract::Effects of methotrexate (MTX) on mitochondrial oxidative metabolism and ion transport were studied. MTX decreases the membrane potential (delta psi) upon energization of the mitochondrial membrane by NAD+-linked substrates and decreases the amplitude and velocity of swelling induced by glutamate and alpha-ketoglutarat...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290070208
更新日期:1989-04-01 00:00:00
abstract::Mitochondrial proton leak can account for almost 20% of oxygen consumption and it is generally accepted that this process contributes to basal metabolism. In order to clarify the role of basal proton leak in testicular mitochondria, we performed a comparative study with kidney and liver mitochondrial fractions. Proton...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1644
更新日期:2010-04-01 00:00:00
abstract::The MCF-7 human breast carcinoma cell line is widely used as a model system by breast cancer researchers and cell biologists investigating apoptosis. Since its establishment 30 years ago, from a patient with metastatic breast cancer, the original MCF-7 cell population has undergone genetic drift to such an extent that...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1106
更新日期:2004-09-01 00:00:00
abstract::The spreading of HeLa cells on plastic substratum is mediated by fibronectin-depleted foetal calf serum but not by fibronectin isolated by gelatin-Sepharose affinity chromatography. The same is true for freshly explanted chick embryonic chondrocytes. In contrast, BHK cell spreading exceeds 67% after 120 min at 37 degr...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290010208
更新日期:1983-07-01 00:00:00
abstract::The effect of citrinin on Ca2+ transport was studied in isolated kidney cortex and liver mitochondria, and baby hamster kidney cultured cells. The mycotoxin significantly inhibited the activity of 2-oxoglutarate and pyruvate dehydrogenases in both kidney cortex and liver mitochondria. Citrinin promoted a decrease in t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290130110
更新日期:1995-03-01 00:00:00
abstract::A method for the quantification of energy consuming processes described by Siems et al. for reticulocytes and by Müller et al. for ascites tumour cells was applied to balance the ATP-consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080407
更新日期:1990-10-01 00:00:00
abstract::Thyroid cells fall into the type of cells functioning during continuous production of high H(2)O(2) concentrations. We studied the effect of H(2)O(2)-induced oxidative stress (0.1, 1.0 and 10.0 mM) on the activities of the key steps of iodide metabolism (uptake, oxidation and organification) in thyrocytes cultivated i...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1452
更新日期:2008-04-01 00:00:00
abstract::Bone tumor incidence in women peaks at age 50-60, coinciding with the menopause. That estrogen (E2) and triiodothyronine (T3) interact in bone metabolism has been well established. However, few data on the action of these hormones are available. Our purpose was to determine the role of E2 and T3 in the expression of b...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1408
更新日期:2008-01-01 00:00:00
abstract::The aim of the present study is to evaluate the proliferation- and migration-enhancing effects of ginseng and its component, ginsenoside (Rg1) on RSC96 Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle fac...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1554
更新日期:2009-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of artemisinin on the proliferation and apoptosis of rat vascular smooth muscle cells (VSMCs). METHOD:Primary rat VSMCs were treated with various doses of artemisinin. Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2995
更新日期:2014-03-01 00:00:00
abstract::Aberrant innate and adaptive immune responsed to allergens and environmental pollutants lead to respiratory allergic disease such as asthma. In this study, we focused on toll-like receptor-4 (TLR4) expressed on airway epithelium to identify house dust mite (HDM)-regulated allergic inflammation via TLR4 signaling pathw...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1697
更新日期:2010-10-01 00:00:00
abstract::Using several physical techniques the surface of articular cartilage has been reported to be structurally different from the deeper layers. In this paper using immunohistochemical methods, the surface has been shown to contain a characteristically different collagen, Type I in contrast to Type II which is the major co...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290010304
更新日期:1983-10-01 00:00:00
abstract::The effect of influenza strains A (H3N2) and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(19970601)15:2<87::AID-CBF7
更新日期:1997-06-01 00:00:00
abstract::The vascular endothelium plays a key role in arterial wall homeostasis by preventing atherosclerotic plaque formation. A primary causal factor of endothelial dysfunction is the reactive oxygen species. Aerobic exercise is ascribed as an important adjuvant therapy in endothelium-dependent cardiovascular disease. Howeve...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1781
更新日期:2011-10-01 00:00:00
abstract::Organochalcogens are extensively produced and employed by industry and agriculture, and the risk of occupational and environmental toxicity to them has been poorly understood. Here, we investigated the acute effect of a new organochalcogen 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one on biochemical and hematologic...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2806
更新日期:2012-06-01 00:00:00