Abstract:
OBJECTIVE:The aim of this study was to evaluate the effect of artemisinin on the proliferation and apoptosis of rat vascular smooth muscle cells (VSMCs). METHOD:Primary rat VSMCs were treated with various doses of artemisinin. Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the messenger RNA and protein expressions of proliferating cell nuclear antigen were determined by reverse-transcription polymerase chain reaction and immunohistochemistry. Apoptosis was measured using annexin V and propidium iodide double staining evaluated by flow cytometry. Protein expression of Bax, Bcl2, and cyclin-dependent kinase 4 was determined by Western blot. RESULTS:After 72 h of treatment, artemisinin significantly inhibited VSMC proliferation in a dose-dependent manner. Treatment with 1 mM artemisinin for 72 h significantly reduced the expression of proliferating cell nuclear antigen messenger RNA. On the other hand, the same treatment increased the apoptosis of VSMCs, the activation of caspase-3, the Bax protein expression, and the Bax/Bcl2 ratio. CONCLUSION:The results suggest that artemisinin can effectively inhibit VSMC proliferation and induce VSMC apoptosis.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Wang HY,Huang RP,Han P,Xue DB,Li HB,Liu B,Shan P,Wang QS,Li KS,Li HLdoi
10.1002/cbf.2995subject
Has Abstractpub_date
2014-03-01 00:00:00pages
201-8issue
2eissn
0263-6484issn
1099-0844journal_volume
32pub_type
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