Abstract:
:Nuclear, chromatin and microsomal fractions were isolated from hepatocytes prepared from rats injected with [32P]O4(2-) and killed subsequently at times between 1 and 48 h. Specific activities of the total phospholipids (PL) were determined for each subcellular fraction. The major points noted were the initial specific activity of the chromatin PL was half that of both nuclear and microsomal PL at 1 h; the first peak of labelling occurred at 6 h in both nuclear and microsomal PL, but was 3 h later (9h) in the chromatin PL; and a second peak of labelling occurred in the chromatin and microsomal PL, but not in those of the nuclei. On fractionation of the PL, the major and most metabolically active components were phosphatidylcholine + phosphatidylethanolamine, whilst sphingomyelin accounted for only about 8 per cent of the total PL. The chromatin and microsomal fractions were somewhat similar in their labelling patterns though with a delayed peaking of activity in the chromatin. This is indicative of a synthesis and transport of PL from the microsomes to the chromatin.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Viola-Magni MP,Gahan PB,Albi E,Iapoce R,Gentilucci PFdoi
10.1002/cbf.290040409subject
Has Abstractpub_date
1986-10-01 00:00:00pages
283-8issue
4eissn
0263-6484issn
1099-0844journal_volume
4pub_type
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