Methotrexate: studies on cellular metabolism. II--Effects on mitochondrial oxidative metabolism and ion transport.

Abstract:

:Effects of methotrexate (MTX) on mitochondrial oxidative metabolism and ion transport were studied. MTX decreases the membrane potential (delta psi) upon energization of the mitochondrial membrane by NAD+-linked substrates and decreases the amplitude and velocity of swelling induced by glutamate and alpha-ketoglutarate. MTX also has an inhibitory effect on the activities of the oxidation enzymes of NAD+-linked substrates without interfering with the oxidation systems of FAD-linked substrates. The effects of MTX could be interpreted as a consequence of a decrease in the ionic conductivity of the mitochondrial inner membrane.

journal_name

Cell Biochem Funct

authors

Yamamoto N,Lopes LC,Campello AP,Klüppel ML

doi

10.1002/cbf.290070208

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

129-34

issue

2

eissn

0263-6484

issn

1099-0844

journal_volume

7

pub_type

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