Methotrexate: studies on cellular metabolism. II--Effects on mitochondrial oxidative metabolism and ion transport.


:Effects of methotrexate (MTX) on mitochondrial oxidative metabolism and ion transport were studied. MTX decreases the membrane potential (delta psi) upon energization of the mitochondrial membrane by NAD+-linked substrates and decreases the amplitude and velocity of swelling induced by glutamate and alpha-ketoglutarate. MTX also has an inhibitory effect on the activities of the oxidation enzymes of NAD+-linked substrates without interfering with the oxidation systems of FAD-linked substrates. The effects of MTX could be interpreted as a consequence of a decrease in the ionic conductivity of the mitochondrial inner membrane.


Cell Biochem Funct


Yamamoto N,Lopes LC,Campello AP,Klüppel ML




Has Abstract


1989-04-01 00:00:00












  • Chemoprotective effects of carnosine against genotoxicity induced by cyclophosphamide in mice bone marrow cells.

    abstract::The protective effects of carnosine as a natural dipeptide were investigated in mouse bone marrow cells against genotoxicity induced by cyclophosphamide. Mice were injected with solutions of carnosine at three different doses (10, 50 and 100 mg kg(-1) bw) for five consecutive days. On the fifth day of treatment, mice ...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Naghshvar F,Abianeh SM,Ahmadashrafi S,Hosseinimehr SJ

    更新日期:2012-10-01 00:00:00

  • Cervico-vaginal dysplasia--papillomavirus-induced and HIV-1 infection: role of correlated markers for prognostic evaluation.

    abstract::Sexually-transmitted diseases (STD) can facilitate the progression of HIV-1 infection. Among them, as we have previously demonstrated, cervico-vaginal dysplasia-papillomavirus (HPV)-induced, together with HSV-2 co-infection, seems to be correlated with a more evident immunodepression in HIV-positive women, compared wi...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Pugliese A,Andronico L,Gennero L,Pagliano G,Gallo G,Torre D

    更新日期:2002-09-01 00:00:00

  • Role of Spm-Cer-S1P signalling pathway in MMP-2 mediated U46619-induced proliferation of pulmonary artery smooth muscle cells: protective role of epigallocatechin-3-gallate.

    abstract::During remodelling of pulmonary artery, marked proliferation of pulmonary artery smooth muscle cells (PASMCs) occurs, which contributes to pulmonary hypertension. Thromboxane A2 (TxA2) has been shown to produce pulmonary hypertension. The present study investigates the inhibitory effect of epigallocatechin-3-gallate (...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Chowdhury A,Sarkar J,Chakraborti T,Chakraborti S

    更新日期:2015-10-01 00:00:00

  • Proliferating cell nuclear antigen (PCNA/cyclin) in plant proliferating cells: immunohistochemical and quantitative analysis using autoantibody and murine monoclonal antibodies to PCNA.

    abstract::Proliferating-cell nuclear antigen (PCNA), also known as cyclin, is synthesized in proliferative cells and recently was identified as DNA polymerase-delta auxiliary protein. In this paper, the association of PCNA to the proliferative cells of plants was analysed using both autoantibodies to PCNA obtained from a patien...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Daidoji H,Takasaki Y,Nakane PK

    更新日期:1992-06-01 00:00:00

  • The assay of uridine diphosphoglucose dehydrogenase activity: discrimination from xanthine dehydrogenase activity.

    abstract::The biochemical and quantitative cytochemical assays of the activity of uridine diphosphoglucose dehydrogenase (UDPG-D) have produced perplexing results. It is now shown that the perplexity may be due to the possibility that the coenzyme (NAD) required for UDPG-D activity, may be acting as a substrate for a second deh...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Mehdizadeh S,Bitensky L,Chayen J

    更新日期:1991-04-01 00:00:00

  • The use of erythrocyte fragility to assess xenobiotic cytotoxicity.

    abstract::The erythrocytes of mammals represent a good model to evaluate the cytotoxicity of molecules, organic and inorganic, natural or synthetic, by cellular damage measure. Indeed, before any investigation on the mechanism of action of different molecules, it is important to perform a cytotoxicity assay. Among the different...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章,评审


    authors: Pagano M,Faggio C

    更新日期:2015-08-01 00:00:00

  • Lipases operative at the fat cell surface: attempt at an integrated approach.

    abstract::Extracellular acylglycerols are hydrolysed by lipases active at the surface of intact fat cells isolated from rat or human adipose tissue. During short-term incubation, rat fat cells hydrolyse di-[3H]oleyl-[14C]glycerol at a rate of 70 +/- 7.7 mU/10(6) cells (mean +/- S.E.) versus 440 +/- 62 mU/10(6) cells for the hyd...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Verine A,Boyer J

    更新日期:1987-07-01 00:00:00

  • Cyclophilin A as a downstream effector of PI3K/Akt signalling pathway in multiple myeloma cells.

    abstract::Cyclophilin A (Cyp A), a member of the peptidyl-prolyl isomerase (PPI) family, may function as a molecular signalling switch. Comparative proteomic studies have identified Cyp A as a potential downstream target of protein kinase B (Akt). This study confirmed that Cyp A is a downstream effector of the phosphatidylinosi...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Lin ZL,Wu HJ,Chen JA,Lin KC,Hsu JH

    更新日期:2015-12-01 00:00:00

  • Oxidative stress and cannabinoid receptor expression in type-2 diabetic rat pancreas following treatment with Δ⁹-THC.

    abstract::The objectives of study were (a) to determine alteration of feeding, glucose level and oxidative stress and (b) to investigate expression and localization of cannabinoid receptors in type-2 diabetic rat pancreas treated with Δ(9)-tetrahydrocannabinol (Δ(9)-THC). Rats were randomly divided into four groups: control, Δ(...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Coskun ZM,Bolkent S

    更新日期:2014-10-01 00:00:00

  • Localization of putative gonadotrophin releasing hormone receptor protein in the anterior pituitary.

    abstract::Specific binding of a fully biologically active 125I-gonadotrophin releasing hormone (GnRH) to isolated anterior pituitary cells is time dependent, saturable and the concentration dependent binding curves exhibit positive cooperativity. Binding to intact or solubilized plasma membranes and an affinity purified GnRH re...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Zolman JC,Theodoropoulos TJ

    更新日期:1984-10-01 00:00:00

  • The protection by heme oxygenase-1 induction against thioacetamide-induced liver toxicity is associated with changes in arginine and asymmetric dimethylarginine.

    abstract::This study was designed to investigate the role of HO-1 induction in prevention of thioacetamide (TAA)-induced oxidative stress, inflammation and liver damage. The changes in hepatic dimethylarginine dimethylaminohydrolase (DDAH) activity as well as plasma arginine and asymmetric dimethylarginine (ADMA) levels were al...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Develi-Is S,Bekpinar S,Kalaz EB,Evran B,Unlucerci Y,Gulluoglu M,Uysal M

    更新日期:2013-03-01 00:00:00

  • Isocitrate dehydrogenase activity and its regulation by estradiol in tissues of rats of various ages.

    abstract::The activity and hormonal regulation of NAD- and NADP-linked isocitrate dehydrogenase (EC. and EC., respectively) in the brain, liver and kidney cortex of female rats of various ages was investigated. The activity of NAD-ICDH of brain was greater than extramitochondrial (-c) or intramitochondrial (-m) ...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Yadav RN

    更新日期:1988-07-01 00:00:00

  • Modulation of gastrin-releasing peptide (GRP) receptors in insulin secreting cells.

    abstract::Gastrin-releasing peptide (GRP) receptors are present in pancreatic islets, though their regulation is unknown except for homologous desensitization. The modulation of binding of GRP to mouse pancreatic islets and INS-1 cells was studied. At 60 min (steady-state), total binding of [(125)I-Tyr(15)] GRP was 1.62 per cen...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Kloss H,Wahl MA,Neye H,Verspohl EJ

    更新日期:1999-12-01 00:00:00

  • Soy lecithin supplementation alters macrophage phagocytosis and lymphocyte response to concanavalin A: a study in alloxan-induced diabetic rats.

    abstract::Dietary soy lecithin supplementation decreases hyperlipidemia and influences lipid metabolism. Although this product is used by diabetic patients, there are no data about the effect of soy lecithin supplementation on the immune system. The addition of phosphatidylcholine, the main component of lecithin, to a culture o...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Miranda DT,Batista VG,Grando FC,Paula FM,Felício CA,Rubbo GF,Fernandes LC,Curi R,Nishiyama A

    更新日期:2008-12-01 00:00:00

  • Leucine improves protein nutritional status and regulates hepatic lipid metabolism in calorie-restricted rats.

    abstract::Several studies have highlighted the potential of leucine supplementation for the treatment of metabolic diseases including type 2 diabetes and obesity. Caloric restriction is a common approach to improve the health in diabetic and obese subjects. However, very few studies assessed the effects of leucine supplementati...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Pedroso JA,Nishimura LS,de Matos-Neto EM,Donato J Jr,Tirapegui J

    更新日期:2014-06-01 00:00:00

  • Balancing of energy-consuming processes of rat hepatocytes.

    abstract::A method for the quantification of energy consuming processes described by Siems et al. for reticulocytes and by Müller et al. for ascites tumour cells was applied to balance the ATP-consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Schneider W,Siems W,Grune T

    更新日期:1990-10-01 00:00:00

  • Dietary vitamin C and E modulates oxidative stress induced-kidney and lens injury in diabetic aged male rats through modulating glucose homeostasis and antioxidant systems.

    abstract::Diabetes induces oxidative stress in aged human and rat, although daily supplementation of vitamins C and E (VCE) can be beneficial to aged diabetic rats by reducing free radical production. The aim of the present study was to evaluate whether dietary VCE supplementation relieves oxidative stress in streptozotocin (ST...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Özkaya D,Naziroğlu M,Armağan A,Demirel A,Köroglu BK,Çolakoğlu N,Kükner A,Sönmez TT

    更新日期:2011-06-01 00:00:00

  • Studies on the endogenous phospholipids of chick embryo myocardium and their in vitro hydrolysis by endogenous phospholipases during embryogenesis.

    abstract::The phospholipid profiles of the myocardium (from 10- and 18-day old chick embryos and 13-day old chick) and their in vitro response to the endogenous lipolytic enzymes (mainly of the phospholipase group) at pH 7.4 and 38 degrees C for 60 min were analyzed by TLC technology and densitometry. Cardiolipin (CL) was shown...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Helmy FM,Aikins A,Hughes J,Belfield C,Juracka A

    更新日期:2007-09-01 00:00:00

  • The neutrophil elastase mutant affects viability and differentiation of the human monocytic THP-1 cell.

    abstract::Deficiency in neutrophils (neutropenia) caused by mutations in neutrophil elastase (NE, ELA2) has been extensively investigated. Monocytes and neutrophils are derived from a common myeloid progenitor; therefore, ELA2 mutations can also influence monocyte development. These effects have not been well described. In this...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Lee HH,Sung MH

    更新日期:2013-06-01 00:00:00

  • Mitochondria as the target for mildronate's protective effects in azidothymidine (AZT)-induced toxicity of isolated rat liver mitochondria.

    abstract::Previously mildronate, an aza-butyrobetaine derivative, was shown to be a cytoprotective drug, through its mechanism of action of inhibition of carnitine palmitoyltransferase-1, thus protecting mitochondria from long-chain fatty acid accumulation and subsequent damage. Recently in an azidothymidine (AZT)-induced cardi...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Pupure J,Fernandes MA,Santos MS,Moreno AJ,Kalvinsh I,Klusa V,Oliveira CR

    更新日期:2008-09-01 00:00:00

  • N-3 but not N-6 fatty acids reduce the expression of the combined adhesion and scavenger receptor CD36 in human monocytic cells.

    abstract::CD36, a multifunctional adhesion receptor e.g. for thrombospondin and collagen, as well as a scavenger receptor for oxidized low density lipoprotein, is expressed e.g. on platelets and monocytes. By this dual role it might be involved in early steps of atherosclerosis like the recruitment of monocytes and formation of...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Pietsch A,Weber C,Goretzki M,Weber PC,Lorenz RL

    更新日期:1995-09-01 00:00:00

  • Pharmacoresistant Cav 2·3 (E-type/R-type) voltage-gated calcium channels influence heart rate dynamics and may contribute to cardiac impulse conduction.

    abstract::Voltage-gated Ca(2+) channels regulate cardiac automaticity, rhythmicity and excitation-contraction coupling. Whereas L-type (Cav 1·2, Cav 1·3) and T-type (Cav 3·1, Cav 3·2) channels are widely accepted for their functional relevance in the heart, the role of Cav 2·3 Ca(2+) channels expressing R-type currents remains ...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Galetin T,Tevoufouet EE,Sandmeyer J,Matthes J,Nguemo F,Hescheler J,Weiergräber M,Schneider T

    更新日期:2013-07-01 00:00:00

  • Effect of serotonin-acting agents on the serotonin content of immune cells. A peculiar observation.

    abstract::The effect of the tryptophan hydroxylase inhibitor, PCPA methylester, the serotonin reuptake inhibitor fluoxetine and MAO-A inhibitor clorgyline on the serotonin content of rat immune cells was studied, using labelled antibodies and flow cytometry. Each molecule significantly increased in males the serotonin concentra...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Pállinger E,Csaba G

    更新日期:2007-09-01 00:00:00

  • Differential expression of long non-coding RNAs in hyperoxia-induced bronchopulmonary dysplasia.

    abstract::Bronchopulmonary dysplasia (BPD) is a common complication of premature birth that seriously affects the survival rate and quality of life among preterm neonates. Long non-coding RNAs (lncRNAs) have been implicated in many human diseases. However, the role of lncRNAs in the pathogenesis of BPD remains poorly understood...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Bao TP,Wu R,Cheng HP,Cui XW,Tian ZF

    更新日期:2016-07-01 00:00:00

  • Spermidine promotes melanin production through an MITF signalling pathway.

    abstract::Melanin plays an important role in determining skin colour. Apoptosis of melanocytes and defect in melanin production cause vitiligo. Various studies have been conducted to treat the disease, but its treatment is still difficult. Therefore, this study aimed to investigate the effect of spermidine, which is known as an...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Kang SH,Kim MM

    更新日期:2021-01-25 00:00:00

  • Effect of oxidative stress on rat thyrocyte iodide metabolism.

    abstract::Thyroid cells fall into the type of cells functioning during continuous production of high H(2)O(2) concentrations. We studied the effect of H(2)O(2)-induced oxidative stress (0.1, 1.0 and 10.0 mM) on the activities of the key steps of iodide metabolism (uptake, oxidation and organification) in thyrocytes cultivated i...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Nadolnik LI,Niatsetskaya ZV,Lupachyk SV

    更新日期:2008-04-01 00:00:00

  • Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies.

    abstract::UFD1L is the human homologue of the yeast ubiquitin fusion degradation 1 (Ufd1) gene and maps on chromosome 22q11.2 in the typically deleted region (TDR) for DiGeorge/velocardiofacial syndromes (DGS/VCFS). In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD pathway). Several s...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Amati F,Condò I,Conti E,Sangiuolo F,Dallapiccola B,Testi R,Novelli G

    更新日期:2003-09-01 00:00:00

  • Antimicrobial and biological effects of ipemphos and amphos on bacterial and yeast strains.

    abstract::In this study, the antimicrobial effects of monophosphazenes such as SM ipemphos and amphos were examined on bacterial and yeast strains. In addition, the biological effects of these compounds were tested on the lipid level of Saccharomyces cerevisiae and Candida albicans cells. The SM has an antimicrobial effect on t...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Oztürk AI,Yilmaz O,Kirbağ S,Arslan M

    更新日期:2000-06-01 00:00:00

  • Cloturin: effect on energy-producing processes in Ehrlich ascites and P388 murine leukaemia cells.

    abstract::The main purpose of the present investigation was to study the effect of cloturin on aerobic glycolysis, endogenous and exogenous respiration and the level of ATP in both Ehrlich ascites carcinoma (EAC) and P388 murine leukaemia cells incubated in vitro. Also its effect on the level of total (T-SH) and non-protein (NP...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Miko M,Krepelka J,Melka M

    更新日期:1992-06-01 00:00:00

  • Up-regulation of semaphorin 4A expression in human retinal pigment epithelial cells by PACAP released from cocultured neural cells.

    abstract::Development and homeostasis of multicellular organisms require interactions between neighbouring cells. We recently established an in vitro model of cell-cell interaction based on a collagen vitrigel membrane. We have now examined the role of neural cells in retinal homeostasis by coculture of human retinal pigment ep...

    journal_title:Cell biochemistry and function

    pub_type: 杂志文章


    authors: Ko JA,Hirata J,Yamane K,Sonoda KH,Kiuchi Y

    更新日期:2015-01-01 00:00:00